Automated method for the total creatinine determination in dehydrated broths

Autores
Acebal, Carolina Cecilia; Centurión, María Eugenia; Lista, Adriana Guillermina; Fernández Band, Beatriz Susana
Año de publicación
2006
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In order to improve the quality control of dehydrated broth, a new automated method was developed to determine total creatinine in dehydrated broths. The sample pretreatment was coupled on-line with the Flow Injection Analysis (FIA) system for analyte determination by the classical Jaffe reaction, stopped flow methodology, and spectrophotometric detection. The time consumed was reduced from 7h, which is necessary with the official method, to 25 min. The calibration graph is linear between 0.342-1.368 mg creatinine/ 100 mL. The relative standard deviation (RSD%) was 1.7%, the sample throughput was 7 h -1, and the detection limit was 0.185 mg creatinine/100 mL. The validation of the proposed method was carried out with real samples. The obtained results were compared with those obtained from the Association of Official Analytical Chemists (AOAC) reference method.
Fil: Acebal, Carolina Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Centurión, María Eugenia. Universidad Nacional del Sur. Departamento de Química; Argentina
Fil: Lista, Adriana Guillermina. Universidad Nacional del Sur. Departamento de Química; Argentina
Fil: Fernández Band, Beatriz Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Materia
AUTOMATED ANALYSIS
CREATININE
FIA
MEAT PRODUCT
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/95350
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/95350
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Automated method for the total creatinine determination in dehydrated brothsAcebal, Carolina CeciliaCenturión, María EugeniaLista, Adriana GuillerminaFernández Band, Beatriz SusanaAUTOMATED ANALYSISCREATININEFIAMEAT PRODUCThttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1In order to improve the quality control of dehydrated broth, a new automated method was developed to determine total creatinine in dehydrated broths. The sample pretreatment was coupled on-line with the Flow Injection Analysis (FIA) system for analyte determination by the classical Jaffe reaction, stopped flow methodology, and spectrophotometric detection. The time consumed was reduced from 7h, which is necessary with the official method, to 25 min. The calibration graph is linear between 0.342-1.368 mg creatinine/ 100 mL. The relative standard deviation (RSD%) was 1.7%, the sample throughput was 7 h -1, and the detection limit was 0.185 mg creatinine/100 mL. The validation of the proposed method was carried out with real samples. The obtained results were compared with those obtained from the Association of Official Analytical Chemists (AOAC) reference method.Fil: Acebal, Carolina Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; ArgentinaFil: Centurión, María Eugenia. Universidad Nacional del Sur. Departamento de Química; ArgentinaFil: Lista, Adriana Guillermina. Universidad Nacional del Sur. Departamento de Química; ArgentinaFil: Fernández Band, Beatriz Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; ArgentinaTaylor & Francis2006-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/95350Acebal, Carolina Cecilia; Centurión, María Eugenia; Lista, Adriana Guillermina; Fernández Band, Beatriz Susana; Automated method for the total creatinine determination in dehydrated broths; Taylor & Francis; Analytical Letters; 39; 2; 1-2006; 387-3940003-2719CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1080/00032710500477175info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/abs/10.1080/00032710500477175info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:53:06Zoai:ri.conicet.gov.ar:11336/95350instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:53:06.552CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Automated method for the total creatinine determination in dehydrated broths
title Automated method for the total creatinine determination in dehydrated broths
spellingShingle Automated method for the total creatinine determination in dehydrated broths
Acebal, Carolina Cecilia
AUTOMATED ANALYSIS
CREATININE
FIA
MEAT PRODUCT
title_short Automated method for the total creatinine determination in dehydrated broths
title_full Automated method for the total creatinine determination in dehydrated broths
title_fullStr Automated method for the total creatinine determination in dehydrated broths
title_full_unstemmed Automated method for the total creatinine determination in dehydrated broths
title_sort Automated method for the total creatinine determination in dehydrated broths
dc.creator.none.fl_str_mv Acebal, Carolina Cecilia
Centurión, María Eugenia
Lista, Adriana Guillermina
Fernández Band, Beatriz Susana
author Acebal, Carolina Cecilia
author_facet Acebal, Carolina Cecilia
Centurión, María Eugenia
Lista, Adriana Guillermina
Fernández Band, Beatriz Susana
author_role author
author2 Centurión, María Eugenia
Lista, Adriana Guillermina
Fernández Band, Beatriz Susana
author2_role author
author
author
dc.subject.none.fl_str_mv AUTOMATED ANALYSIS
CREATININE
FIA
MEAT PRODUCT
topic AUTOMATED ANALYSIS
CREATININE
FIA
MEAT PRODUCT
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In order to improve the quality control of dehydrated broth, a new automated method was developed to determine total creatinine in dehydrated broths. The sample pretreatment was coupled on-line with the Flow Injection Analysis (FIA) system for analyte determination by the classical Jaffe reaction, stopped flow methodology, and spectrophotometric detection. The time consumed was reduced from 7h, which is necessary with the official method, to 25 min. The calibration graph is linear between 0.342-1.368 mg creatinine/ 100 mL. The relative standard deviation (RSD%) was 1.7%, the sample throughput was 7 h -1, and the detection limit was 0.185 mg creatinine/100 mL. The validation of the proposed method was carried out with real samples. The obtained results were compared with those obtained from the Association of Official Analytical Chemists (AOAC) reference method.
Fil: Acebal, Carolina Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Centurión, María Eugenia. Universidad Nacional del Sur. Departamento de Química; Argentina
Fil: Lista, Adriana Guillermina. Universidad Nacional del Sur. Departamento de Química; Argentina
Fil: Fernández Band, Beatriz Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
description In order to improve the quality control of dehydrated broth, a new automated method was developed to determine total creatinine in dehydrated broths. The sample pretreatment was coupled on-line with the Flow Injection Analysis (FIA) system for analyte determination by the classical Jaffe reaction, stopped flow methodology, and spectrophotometric detection. The time consumed was reduced from 7h, which is necessary with the official method, to 25 min. The calibration graph is linear between 0.342-1.368 mg creatinine/ 100 mL. The relative standard deviation (RSD%) was 1.7%, the sample throughput was 7 h -1, and the detection limit was 0.185 mg creatinine/100 mL. The validation of the proposed method was carried out with real samples. The obtained results were compared with those obtained from the Association of Official Analytical Chemists (AOAC) reference method.
publishDate 2006
dc.date.none.fl_str_mv 2006-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/95350
Acebal, Carolina Cecilia; Centurión, María Eugenia; Lista, Adriana Guillermina; Fernández Band, Beatriz Susana; Automated method for the total creatinine determination in dehydrated broths; Taylor & Francis; Analytical Letters; 39; 2; 1-2006; 387-394
0003-2719
CONICET Digital
CONICET
url http://hdl.handle.net/11336/95350
identifier_str_mv Acebal, Carolina Cecilia; Centurión, María Eugenia; Lista, Adriana Guillermina; Fernández Band, Beatriz Susana; Automated method for the total creatinine determination in dehydrated broths; Taylor & Francis; Analytical Letters; 39; 2; 1-2006; 387-394
0003-2719
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1080/00032710500477175
info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/abs/10.1080/00032710500477175
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Taylor & Francis
publisher.none.fl_str_mv Taylor & Francis
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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