Receptor-mediated endocytosis and trafficking between endosomal–lysosomal vacuoles in Giardia lamblia

Autores
Rivero, Maria Romina; Jausoro, Ignacio; Bisbal, Mariano; Feliziani, Constanza; Lanfredi Rangel, Adriana; Touz, Maria Carolina
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The early branching Giardia lamblia has highly polarized vacuoles, located underneath the plasma membrane, which have at least some of the characteristics of endosomes and of lysosomes. These peripheral vacuoles (PVs) are necessary for nutrient uptake and the maintenance of plasma membrane composition, but whether they carry out sorting and segregation of receptors and ligands is a matter of debate. Here, we showed that the internalization of low-density lipoprotein (LDL) to the PVs is highly dynamic in trophozoites with a rate similar to the internalization of the low-density lipoprotein receptor-related protein 1. Moreover, by analyzing receptor-mediated and fluid-phase endocytosis in living cells, we showed that after endocytosis LDL but not dextran moved laterally between the PVs. We speculate on PV functional heterogeneity and maturation in this parasite.
Fil: Rivero, Maria Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto de Investigaciones Médicas Mercedes y Martín Ferreyra; Argentina
Fil: Jausoro, Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto de Investigaciones Médicas Mercedes y Martín Ferreyra; Argentina
Fil: Bisbal, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto de Investigaciones Médicas Mercedes y Martín Ferreyra; Argentina
Fil: Feliziani, Constanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto de Investigaciones Médicas Mercedes y Martín Ferreyra; Argentina
Fil: Lanfredi Rangel, Adriana. Centro de Pesquisas Gonçalo Moniz, Serviço de Microscopia Eletrônica; Brasil
Fil: Touz, Maria Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto de Investigaciones Médicas Mercedes y Martín Ferreyra; Argentina
Materia
GIARDIA i
LAMBLIA
ENDOCYTOSIS
TIRF
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/8896
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/8896
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Receptor-mediated endocytosis and trafficking between endosomal–lysosomal vacuoles in Giardia lambliaRivero, Maria RominaJausoro, IgnacioBisbal, MarianoFeliziani, ConstanzaLanfredi Rangel, AdrianaTouz, Maria CarolinaGIARDIA iLAMBLIAENDOCYTOSISTIRFhttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3The early branching Giardia lamblia has highly polarized vacuoles, located underneath the plasma membrane, which have at least some of the characteristics of endosomes and of lysosomes. These peripheral vacuoles (PVs) are necessary for nutrient uptake and the maintenance of plasma membrane composition, but whether they carry out sorting and segregation of receptors and ligands is a matter of debate. Here, we showed that the internalization of low-density lipoprotein (LDL) to the PVs is highly dynamic in trophozoites with a rate similar to the internalization of the low-density lipoprotein receptor-related protein 1. Moreover, by analyzing receptor-mediated and fluid-phase endocytosis in living cells, we showed that after endocytosis LDL but not dextran moved laterally between the PVs. We speculate on PV functional heterogeneity and maturation in this parasite.Fil: Rivero, Maria Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto de Investigaciones Médicas Mercedes y Martín Ferreyra; ArgentinaFil: Jausoro, Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto de Investigaciones Médicas Mercedes y Martín Ferreyra; ArgentinaFil: Bisbal, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto de Investigaciones Médicas Mercedes y Martín Ferreyra; ArgentinaFil: Feliziani, Constanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto de Investigaciones Médicas Mercedes y Martín Ferreyra; ArgentinaFil: Lanfredi Rangel, Adriana. Centro de Pesquisas Gonçalo Moniz, Serviço de Microscopia Eletrônica; BrasilFil: Touz, Maria Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto de Investigaciones Médicas Mercedes y Martín Ferreyra; ArgentinaSpringer2013-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/8896Rivero, Maria Romina; Jausoro, Ignacio; Bisbal, Mariano; Feliziani, Constanza; Lanfredi Rangel, Adriana; et al.; Receptor-mediated endocytosis and trafficking between endosomal–lysosomal vacuoles in Giardia lamblia; Springer; Parasitology Research; 112; 4; 4-2013; 1813-18180932-0113enginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s00436-012-3253-7info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3600075/info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007%2Fs00436-012-3253-7info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:09:06Zoai:ri.conicet.gov.ar:11336/8896instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:09:06.397CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Receptor-mediated endocytosis and trafficking between endosomal–lysosomal vacuoles in Giardia lamblia
title Receptor-mediated endocytosis and trafficking between endosomal–lysosomal vacuoles in Giardia lamblia
spellingShingle Receptor-mediated endocytosis and trafficking between endosomal–lysosomal vacuoles in Giardia lamblia
Rivero, Maria Romina
GIARDIA i
LAMBLIA
ENDOCYTOSIS
TIRF
title_short Receptor-mediated endocytosis and trafficking between endosomal–lysosomal vacuoles in Giardia lamblia
title_full Receptor-mediated endocytosis and trafficking between endosomal–lysosomal vacuoles in Giardia lamblia
title_fullStr Receptor-mediated endocytosis and trafficking between endosomal–lysosomal vacuoles in Giardia lamblia
title_full_unstemmed Receptor-mediated endocytosis and trafficking between endosomal–lysosomal vacuoles in Giardia lamblia
title_sort Receptor-mediated endocytosis and trafficking between endosomal–lysosomal vacuoles in Giardia lamblia
dc.creator.none.fl_str_mv Rivero, Maria Romina
Jausoro, Ignacio
Bisbal, Mariano
Feliziani, Constanza
Lanfredi Rangel, Adriana
Touz, Maria Carolina
author Rivero, Maria Romina
author_facet Rivero, Maria Romina
Jausoro, Ignacio
Bisbal, Mariano
Feliziani, Constanza
Lanfredi Rangel, Adriana
Touz, Maria Carolina
author_role author
author2 Jausoro, Ignacio
Bisbal, Mariano
Feliziani, Constanza
Lanfredi Rangel, Adriana
Touz, Maria Carolina
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv GIARDIA i
LAMBLIA
ENDOCYTOSIS
TIRF
topic GIARDIA i
LAMBLIA
ENDOCYTOSIS
TIRF
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The early branching Giardia lamblia has highly polarized vacuoles, located underneath the plasma membrane, which have at least some of the characteristics of endosomes and of lysosomes. These peripheral vacuoles (PVs) are necessary for nutrient uptake and the maintenance of plasma membrane composition, but whether they carry out sorting and segregation of receptors and ligands is a matter of debate. Here, we showed that the internalization of low-density lipoprotein (LDL) to the PVs is highly dynamic in trophozoites with a rate similar to the internalization of the low-density lipoprotein receptor-related protein 1. Moreover, by analyzing receptor-mediated and fluid-phase endocytosis in living cells, we showed that after endocytosis LDL but not dextran moved laterally between the PVs. We speculate on PV functional heterogeneity and maturation in this parasite.
Fil: Rivero, Maria Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto de Investigaciones Médicas Mercedes y Martín Ferreyra; Argentina
Fil: Jausoro, Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto de Investigaciones Médicas Mercedes y Martín Ferreyra; Argentina
Fil: Bisbal, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto de Investigaciones Médicas Mercedes y Martín Ferreyra; Argentina
Fil: Feliziani, Constanza. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto de Investigaciones Médicas Mercedes y Martín Ferreyra; Argentina
Fil: Lanfredi Rangel, Adriana. Centro de Pesquisas Gonçalo Moniz, Serviço de Microscopia Eletrônica; Brasil
Fil: Touz, Maria Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto de Investigaciones Médicas Mercedes y Martín Ferreyra; Argentina
description The early branching Giardia lamblia has highly polarized vacuoles, located underneath the plasma membrane, which have at least some of the characteristics of endosomes and of lysosomes. These peripheral vacuoles (PVs) are necessary for nutrient uptake and the maintenance of plasma membrane composition, but whether they carry out sorting and segregation of receptors and ligands is a matter of debate. Here, we showed that the internalization of low-density lipoprotein (LDL) to the PVs is highly dynamic in trophozoites with a rate similar to the internalization of the low-density lipoprotein receptor-related protein 1. Moreover, by analyzing receptor-mediated and fluid-phase endocytosis in living cells, we showed that after endocytosis LDL but not dextran moved laterally between the PVs. We speculate on PV functional heterogeneity and maturation in this parasite.
publishDate 2013
dc.date.none.fl_str_mv 2013-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/8896
Rivero, Maria Romina; Jausoro, Ignacio; Bisbal, Mariano; Feliziani, Constanza; Lanfredi Rangel, Adriana; et al.; Receptor-mediated endocytosis and trafficking between endosomal–lysosomal vacuoles in Giardia lamblia; Springer; Parasitology Research; 112; 4; 4-2013; 1813-1818
0932-0113
url http://hdl.handle.net/11336/8896
identifier_str_mv Rivero, Maria Romina; Jausoro, Ignacio; Bisbal, Mariano; Feliziani, Constanza; Lanfredi Rangel, Adriana; et al.; Receptor-mediated endocytosis and trafficking between endosomal–lysosomal vacuoles in Giardia lamblia; Springer; Parasitology Research; 112; 4; 4-2013; 1813-1818
0932-0113
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s00436-012-3253-7
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3600075/
info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007%2Fs00436-012-3253-7
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842270069219917824
score 13.13397

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