Menadione modulates adipogenic differentiation by inhibition of PI3K/Akt pathway in 3T3-L1 cells

Autores
Iglesias González, Pablo Andrés; Conde, Melisa Ailén; Salvador, Gabriela Alejandra; Uranga, Romina Maria
Año de publicación
2018
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
We have previously demonstrated that menadione-induced oxidative stress significantly diminishes adipogenesis and is able to dephosphorylate and inactivate phosphatidylinositol 3-kinase (PI3K)/Akt pathway in 3T3-L1 cells (SAIB 2017). To investigate this unexpected behavior of this adipogenic key pathway against oxidative stress, we mimicked menadione effect by studying adipogenic differentiation in the presence of LY294002, a well known PI3K inhibitor. In the absence of menadione, we found that PI3K inhibition drastically decreased adipogenesis. At a molecular level, the expression of peroxisome proliferator activated receptor gamma (PPAR gamma), the master regulator of adipogenesis, was also found to be decreased. To investigate if PI3K/Akt pathway was responsible of menadione-caused adipogenesis inhibition, we used insulin in the presence of menadione as a PI3K gain-of-function strategy, both being present during the whole differentiation process. These experiments showed that insulin was sufficient to rescue PPAR gamma expression, without altering cell viability. These results show that PI3K/Akt is a key pathway in the antiadipogenic effect of menadione on 3T3-L1 cells.
Fil: Iglesias González, Pablo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Conde, Melisa Ailén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Salvador, Gabriela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Uranga, Romina Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
LIV Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Paraná
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Materia
MENADIONE
OXIDATIVE STRESS
ADIPOGENESIS
3T3-L1 cells
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/231623
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Menadione modulates adipogenic differentiation by inhibition of PI3K/Akt pathway in 3T3-L1 cellsIglesias González, Pablo AndrésConde, Melisa AilénSalvador, Gabriela AlejandraUranga, Romina MariaMENADIONEOXIDATIVE STRESSADIPOGENESIS3T3-L1 cellshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1We have previously demonstrated that menadione-induced oxidative stress significantly diminishes adipogenesis and is able to dephosphorylate and inactivate phosphatidylinositol 3-kinase (PI3K)/Akt pathway in 3T3-L1 cells (SAIB 2017). To investigate this unexpected behavior of this adipogenic key pathway against oxidative stress, we mimicked menadione effect by studying adipogenic differentiation in the presence of LY294002, a well known PI3K inhibitor. In the absence of menadione, we found that PI3K inhibition drastically decreased adipogenesis. At a molecular level, the expression of peroxisome proliferator activated receptor gamma (PPAR gamma), the master regulator of adipogenesis, was also found to be decreased. To investigate if PI3K/Akt pathway was responsible of menadione-caused adipogenesis inhibition, we used insulin in the presence of menadione as a PI3K gain-of-function strategy, both being present during the whole differentiation process. These experiments showed that insulin was sufficient to rescue PPAR gamma expression, without altering cell viability. These results show that PI3K/Akt is a key pathway in the antiadipogenic effect of menadione on 3T3-L1 cells.Fil: Iglesias González, Pablo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Conde, Melisa Ailén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Salvador, Gabriela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Uranga, Romina Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaLIV Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología MolecularParanáArgentinaSociedad Argentina de Investigación en Bioquímica y Biología MolecularTech Science Press2018info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/231623Menadione modulates adipogenic differentiation by inhibition of PI3K/Akt pathway in 3T3-L1 cells; LIV Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Paraná; Argentina; 2018; 91-910327-95451667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.techscience.com/biocell/v42nSuppl.4/33869Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:47:11Zoai:ri.conicet.gov.ar:11336/231623instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:47:12.064CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Menadione modulates adipogenic differentiation by inhibition of PI3K/Akt pathway in 3T3-L1 cells
title Menadione modulates adipogenic differentiation by inhibition of PI3K/Akt pathway in 3T3-L1 cells
spellingShingle Menadione modulates adipogenic differentiation by inhibition of PI3K/Akt pathway in 3T3-L1 cells
Iglesias González, Pablo Andrés
MENADIONE
OXIDATIVE STRESS
ADIPOGENESIS
3T3-L1 cells
title_short Menadione modulates adipogenic differentiation by inhibition of PI3K/Akt pathway in 3T3-L1 cells
title_full Menadione modulates adipogenic differentiation by inhibition of PI3K/Akt pathway in 3T3-L1 cells
title_fullStr Menadione modulates adipogenic differentiation by inhibition of PI3K/Akt pathway in 3T3-L1 cells
title_full_unstemmed Menadione modulates adipogenic differentiation by inhibition of PI3K/Akt pathway in 3T3-L1 cells
title_sort Menadione modulates adipogenic differentiation by inhibition of PI3K/Akt pathway in 3T3-L1 cells
dc.creator.none.fl_str_mv Iglesias González, Pablo Andrés
Conde, Melisa Ailén
Salvador, Gabriela Alejandra
Uranga, Romina Maria
author Iglesias González, Pablo Andrés
author_facet Iglesias González, Pablo Andrés
Conde, Melisa Ailén
Salvador, Gabriela Alejandra
Uranga, Romina Maria
author_role author
author2 Conde, Melisa Ailén
Salvador, Gabriela Alejandra
Uranga, Romina Maria
author2_role author
author
author
dc.subject.none.fl_str_mv MENADIONE
OXIDATIVE STRESS
ADIPOGENESIS
3T3-L1 cells
topic MENADIONE
OXIDATIVE STRESS
ADIPOGENESIS
3T3-L1 cells
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv We have previously demonstrated that menadione-induced oxidative stress significantly diminishes adipogenesis and is able to dephosphorylate and inactivate phosphatidylinositol 3-kinase (PI3K)/Akt pathway in 3T3-L1 cells (SAIB 2017). To investigate this unexpected behavior of this adipogenic key pathway against oxidative stress, we mimicked menadione effect by studying adipogenic differentiation in the presence of LY294002, a well known PI3K inhibitor. In the absence of menadione, we found that PI3K inhibition drastically decreased adipogenesis. At a molecular level, the expression of peroxisome proliferator activated receptor gamma (PPAR gamma), the master regulator of adipogenesis, was also found to be decreased. To investigate if PI3K/Akt pathway was responsible of menadione-caused adipogenesis inhibition, we used insulin in the presence of menadione as a PI3K gain-of-function strategy, both being present during the whole differentiation process. These experiments showed that insulin was sufficient to rescue PPAR gamma expression, without altering cell viability. These results show that PI3K/Akt is a key pathway in the antiadipogenic effect of menadione on 3T3-L1 cells.
Fil: Iglesias González, Pablo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Conde, Melisa Ailén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Salvador, Gabriela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Uranga, Romina Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
LIV Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Paraná
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
description We have previously demonstrated that menadione-induced oxidative stress significantly diminishes adipogenesis and is able to dephosphorylate and inactivate phosphatidylinositol 3-kinase (PI3K)/Akt pathway in 3T3-L1 cells (SAIB 2017). To investigate this unexpected behavior of this adipogenic key pathway against oxidative stress, we mimicked menadione effect by studying adipogenic differentiation in the presence of LY294002, a well known PI3K inhibitor. In the absence of menadione, we found that PI3K inhibition drastically decreased adipogenesis. At a molecular level, the expression of peroxisome proliferator activated receptor gamma (PPAR gamma), the master regulator of adipogenesis, was also found to be decreased. To investigate if PI3K/Akt pathway was responsible of menadione-caused adipogenesis inhibition, we used insulin in the presence of menadione as a PI3K gain-of-function strategy, both being present during the whole differentiation process. These experiments showed that insulin was sufficient to rescue PPAR gamma expression, without altering cell viability. These results show that PI3K/Akt is a key pathway in the antiadipogenic effect of menadione on 3T3-L1 cells.
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/231623
Menadione modulates adipogenic differentiation by inhibition of PI3K/Akt pathway in 3T3-L1 cells; LIV Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Paraná; Argentina; 2018; 91-91
0327-9545
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/231623
identifier_str_mv Menadione modulates adipogenic differentiation by inhibition of PI3K/Akt pathway in 3T3-L1 cells; LIV Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Paraná; Argentina; 2018; 91-91
0327-9545
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.techscience.com/biocell/v42nSuppl.4/33869
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
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dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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