Menadione modulates adipogenic differentiation by inhibition of PI3K/Akt pathway in 3T3-L1 cells
- Autores
- Iglesias González, Pablo Andrés; Conde, Melisa Ailén; Salvador, Gabriela Alejandra; Uranga, Romina Maria
- Año de publicación
- 2018
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- We have previously demonstrated that menadione-induced oxidative stress significantly diminishes adipogenesis and is able to dephosphorylate and inactivate phosphatidylinositol 3-kinase (PI3K)/Akt pathway in 3T3-L1 cells (SAIB 2017). To investigate this unexpected behavior of this adipogenic key pathway against oxidative stress, we mimicked menadione effect by studying adipogenic differentiation in the presence of LY294002, a well known PI3K inhibitor. In the absence of menadione, we found that PI3K inhibition drastically decreased adipogenesis. At a molecular level, the expression of peroxisome proliferator activated receptor gamma (PPAR gamma), the master regulator of adipogenesis, was also found to be decreased. To investigate if PI3K/Akt pathway was responsible of menadione-caused adipogenesis inhibition, we used insulin in the presence of menadione as a PI3K gain-of-function strategy, both being present during the whole differentiation process. These experiments showed that insulin was sufficient to rescue PPAR gamma expression, without altering cell viability. These results show that PI3K/Akt is a key pathway in the antiadipogenic effect of menadione on 3T3-L1 cells.
Fil: Iglesias González, Pablo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Conde, Melisa Ailén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Salvador, Gabriela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Uranga, Romina Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
LIV Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Paraná
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular - Materia
-
MENADIONE
OXIDATIVE STRESS
ADIPOGENESIS
3T3-L1 cells - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/231623
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Menadione modulates adipogenic differentiation by inhibition of PI3K/Akt pathway in 3T3-L1 cellsIglesias González, Pablo AndrésConde, Melisa AilénSalvador, Gabriela AlejandraUranga, Romina MariaMENADIONEOXIDATIVE STRESSADIPOGENESIS3T3-L1 cellshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1We have previously demonstrated that menadione-induced oxidative stress significantly diminishes adipogenesis and is able to dephosphorylate and inactivate phosphatidylinositol 3-kinase (PI3K)/Akt pathway in 3T3-L1 cells (SAIB 2017). To investigate this unexpected behavior of this adipogenic key pathway against oxidative stress, we mimicked menadione effect by studying adipogenic differentiation in the presence of LY294002, a well known PI3K inhibitor. In the absence of menadione, we found that PI3K inhibition drastically decreased adipogenesis. At a molecular level, the expression of peroxisome proliferator activated receptor gamma (PPAR gamma), the master regulator of adipogenesis, was also found to be decreased. To investigate if PI3K/Akt pathway was responsible of menadione-caused adipogenesis inhibition, we used insulin in the presence of menadione as a PI3K gain-of-function strategy, both being present during the whole differentiation process. These experiments showed that insulin was sufficient to rescue PPAR gamma expression, without altering cell viability. These results show that PI3K/Akt is a key pathway in the antiadipogenic effect of menadione on 3T3-L1 cells.Fil: Iglesias González, Pablo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Conde, Melisa Ailén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Salvador, Gabriela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Uranga, Romina Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaLIV Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología MolecularParanáArgentinaSociedad Argentina de Investigación en Bioquímica y Biología MolecularTech Science Press2018info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/231623Menadione modulates adipogenic differentiation by inhibition of PI3K/Akt pathway in 3T3-L1 cells; LIV Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Paraná; Argentina; 2018; 91-910327-95451667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.techscience.com/biocell/v42nSuppl.4/33869Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:11:54Zoai:ri.conicet.gov.ar:11336/231623instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:11:54.88CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Menadione modulates adipogenic differentiation by inhibition of PI3K/Akt pathway in 3T3-L1 cells |
title |
Menadione modulates adipogenic differentiation by inhibition of PI3K/Akt pathway in 3T3-L1 cells |
spellingShingle |
Menadione modulates adipogenic differentiation by inhibition of PI3K/Akt pathway in 3T3-L1 cells Iglesias González, Pablo Andrés MENADIONE OXIDATIVE STRESS ADIPOGENESIS 3T3-L1 cells |
title_short |
Menadione modulates adipogenic differentiation by inhibition of PI3K/Akt pathway in 3T3-L1 cells |
title_full |
Menadione modulates adipogenic differentiation by inhibition of PI3K/Akt pathway in 3T3-L1 cells |
title_fullStr |
Menadione modulates adipogenic differentiation by inhibition of PI3K/Akt pathway in 3T3-L1 cells |
title_full_unstemmed |
Menadione modulates adipogenic differentiation by inhibition of PI3K/Akt pathway in 3T3-L1 cells |
title_sort |
Menadione modulates adipogenic differentiation by inhibition of PI3K/Akt pathway in 3T3-L1 cells |
dc.creator.none.fl_str_mv |
Iglesias González, Pablo Andrés Conde, Melisa Ailén Salvador, Gabriela Alejandra Uranga, Romina Maria |
author |
Iglesias González, Pablo Andrés |
author_facet |
Iglesias González, Pablo Andrés Conde, Melisa Ailén Salvador, Gabriela Alejandra Uranga, Romina Maria |
author_role |
author |
author2 |
Conde, Melisa Ailén Salvador, Gabriela Alejandra Uranga, Romina Maria |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
MENADIONE OXIDATIVE STRESS ADIPOGENESIS 3T3-L1 cells |
topic |
MENADIONE OXIDATIVE STRESS ADIPOGENESIS 3T3-L1 cells |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
We have previously demonstrated that menadione-induced oxidative stress significantly diminishes adipogenesis and is able to dephosphorylate and inactivate phosphatidylinositol 3-kinase (PI3K)/Akt pathway in 3T3-L1 cells (SAIB 2017). To investigate this unexpected behavior of this adipogenic key pathway against oxidative stress, we mimicked menadione effect by studying adipogenic differentiation in the presence of LY294002, a well known PI3K inhibitor. In the absence of menadione, we found that PI3K inhibition drastically decreased adipogenesis. At a molecular level, the expression of peroxisome proliferator activated receptor gamma (PPAR gamma), the master regulator of adipogenesis, was also found to be decreased. To investigate if PI3K/Akt pathway was responsible of menadione-caused adipogenesis inhibition, we used insulin in the presence of menadione as a PI3K gain-of-function strategy, both being present during the whole differentiation process. These experiments showed that insulin was sufficient to rescue PPAR gamma expression, without altering cell viability. These results show that PI3K/Akt is a key pathway in the antiadipogenic effect of menadione on 3T3-L1 cells. Fil: Iglesias González, Pablo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina Fil: Conde, Melisa Ailén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina Fil: Salvador, Gabriela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina Fil: Uranga, Romina Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina LIV Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular Paraná Argentina Sociedad Argentina de Investigación en Bioquímica y Biología Molecular |
description |
We have previously demonstrated that menadione-induced oxidative stress significantly diminishes adipogenesis and is able to dephosphorylate and inactivate phosphatidylinositol 3-kinase (PI3K)/Akt pathway in 3T3-L1 cells (SAIB 2017). To investigate this unexpected behavior of this adipogenic key pathway against oxidative stress, we mimicked menadione effect by studying adipogenic differentiation in the presence of LY294002, a well known PI3K inhibitor. In the absence of menadione, we found that PI3K inhibition drastically decreased adipogenesis. At a molecular level, the expression of peroxisome proliferator activated receptor gamma (PPAR gamma), the master regulator of adipogenesis, was also found to be decreased. To investigate if PI3K/Akt pathway was responsible of menadione-caused adipogenesis inhibition, we used insulin in the presence of menadione as a PI3K gain-of-function strategy, both being present during the whole differentiation process. These experiments showed that insulin was sufficient to rescue PPAR gamma expression, without altering cell viability. These results show that PI3K/Akt is a key pathway in the antiadipogenic effect of menadione on 3T3-L1 cells. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/conferenceObject Reunión Journal http://purl.org/coar/resource_type/c_5794 info:ar-repo/semantics/documentoDeConferencia |
status_str |
publishedVersion |
format |
conferenceObject |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/231623 Menadione modulates adipogenic differentiation by inhibition of PI3K/Akt pathway in 3T3-L1 cells; LIV Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Paraná; Argentina; 2018; 91-91 0327-9545 1667-5746 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/231623 |
identifier_str_mv |
Menadione modulates adipogenic differentiation by inhibition of PI3K/Akt pathway in 3T3-L1 cells; LIV Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Paraná; Argentina; 2018; 91-91 0327-9545 1667-5746 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
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eng |
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Tech Science Press |
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Tech Science Press |
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