The regulation of proteins14-3-3 and the hippo pathway affect the adipogenesis or 3T3-L1

Autores
del Veliz, Samanta; Uhart, Marina; Lim, Gareth; Bustos, Diego Martin
Año de publicación
2019
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Many transcription factors act sequentially to activate adipocyte differentiation. Multiple signal transduction pathways govern the adipocyte physiology. The Hippo kinase pathway is the main regulator of proliferation and differentiation of stem cells and adipocyte precursor cells. The 14-3-3 protein family, comprised of 7 paralogs in mammals, interacts with components of the Hippo kinase pathway regulating the adipogenic differentiation, although the specificity of these proteins in the process remains elusive. Deciphering the subcellular dynamics of 14-3-3 proteins will be instrumental in the discovery of new targets for the manipulation of stem cell fate decisions or treatment of chronic diseases, such as obesity and type 2 diabetes. In vitro adipocyte differentiation occurs by the addition of an Adipogenic Differentiation Medium (ADM) including Dulbecco's Modified Eagle Medium, 10% Fetal Bovine Serum, synthetic drugs (dexamethasone, IBMX, rosiglitazone) and peptide hormones (insulin). Since these mentioned chemical drugs are not present in physiological environments, it would be important to achieve a correct activated adipogenic program using fewer drugs and with natural origin. We have decided to evaluate the adipogenic potential of glucagon-like peptide 1 (GLP-1) analogs. In contrast to Native GLP-1 action, which is rapidly degraded by circulating Dipeptidyl Peptidase-4, GLP-1 analogs have shown positive effects on glycemic control and body weight due to their greater stability and longer half-life. In this project, experiments were performed on 3T3- L1 preadipocytes. First, the effect of different combinations of drugs for 7 days in adipogenesis was evaluated. Then, we carried out qPCR experiments to measure the gene expression of 14-3-3 and the most important proteins of the Hippo pathway, on days 3 and 7 of differentiation. We have determined that conditions resulting in greater adipogenic differentiation showed higher levels of Hippo pathway proteins and 14-3-3 gamma and beta isoforms on day 7. These effects were especially evident when IBMX was replaced by GLP-1 in the ADM. These results suggest that different inducers (glucocorticoids, thiazolidinediones, incretins), have different abilities for regulating the expression of 14-3-3 and hippo pathway proteins, thus affecting adipogenic differentiation. We are currently focused on elucidating the mechanisms of action of these drugs to have a greater understanding of the events that are necessary for adipogenesis to occur.
Fil: del Veliz, Samanta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Uhart, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Lim, Gareth.  University of Montreal Hospital Research Centre; Canadá
Fil: Bustos, Diego Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
LV Reunión Anual de la Sociedad Argentina de Investigaciones en Bioquímica; XIV Congreso de la Federación Panamericana de Bioquímica y Biología Molecular
Salta
Argentina
Sociedad Argentina de Investigaciones en Bioquímica
Federación Panamericana de Bioquímica y Biología Molecular
Materia
14-3-3
HIPPO PATHWAY
ADIPOGENESIS
GLP-1
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/235881
Acceder
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network_name_str CONICET Digital (CONICET)
spelling The regulation of proteins14-3-3 and the hippo pathway affect the adipogenesis or 3T3-L1del Veliz, SamantaUhart, MarinaLim, GarethBustos, Diego Martin14-3-3HIPPO PATHWAYADIPOGENESISGLP-1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Many transcription factors act sequentially to activate adipocyte differentiation. Multiple signal transduction pathways govern the adipocyte physiology. The Hippo kinase pathway is the main regulator of proliferation and differentiation of stem cells and adipocyte precursor cells. The 14-3-3 protein family, comprised of 7 paralogs in mammals, interacts with components of the Hippo kinase pathway regulating the adipogenic differentiation, although the specificity of these proteins in the process remains elusive. Deciphering the subcellular dynamics of 14-3-3 proteins will be instrumental in the discovery of new targets for the manipulation of stem cell fate decisions or treatment of chronic diseases, such as obesity and type 2 diabetes. In vitro adipocyte differentiation occurs by the addition of an Adipogenic Differentiation Medium (ADM) including Dulbecco's Modified Eagle Medium, 10% Fetal Bovine Serum, synthetic drugs (dexamethasone, IBMX, rosiglitazone) and peptide hormones (insulin). Since these mentioned chemical drugs are not present in physiological environments, it would be important to achieve a correct activated adipogenic program using fewer drugs and with natural origin. We have decided to evaluate the adipogenic potential of glucagon-like peptide 1 (GLP-1) analogs. In contrast to Native GLP-1 action, which is rapidly degraded by circulating Dipeptidyl Peptidase-4, GLP-1 analogs have shown positive effects on glycemic control and body weight due to their greater stability and longer half-life. In this project, experiments were performed on 3T3- L1 preadipocytes. First, the effect of different combinations of drugs for 7 days in adipogenesis was evaluated. Then, we carried out qPCR experiments to measure the gene expression of 14-3-3 and the most important proteins of the Hippo pathway, on days 3 and 7 of differentiation. We have determined that conditions resulting in greater adipogenic differentiation showed higher levels of Hippo pathway proteins and 14-3-3 gamma and beta isoforms on day 7. These effects were especially evident when IBMX was replaced by GLP-1 in the ADM. These results suggest that different inducers (glucocorticoids, thiazolidinediones, incretins), have different abilities for regulating the expression of 14-3-3 and hippo pathway proteins, thus affecting adipogenic differentiation. We are currently focused on elucidating the mechanisms of action of these drugs to have a greater understanding of the events that are necessary for adipogenesis to occur.Fil: del Veliz, Samanta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Uhart, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Lim, Gareth.  University of Montreal Hospital Research Centre; CanadáFil: Bustos, Diego Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaLV Reunión Anual de la Sociedad Argentina de Investigaciones en Bioquímica; XIV Congreso de la Federación Panamericana de Bioquímica y Biología MolecularSaltaArgentinaSociedad Argentina de Investigaciones en BioquímicaFederación Panamericana de Bioquímica y Biología MolecularTech Science Press2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfhttp://hdl.handle.net/11336/235881The regulation of proteins14-3-3 and the hippo pathway affect the adipogenesis or 3T3-L1; LV Reunión Anual de la Sociedad Argentina de Investigaciones en Bioquímica; XIV Congreso de la Federación Panamericana de Bioquímica y Biología Molecular; Salta; Argentina; 2019; 52-520327-95451667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.techscience.com/biocell/v43nSuppl.5/33868/pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:52:46Zoai:ri.conicet.gov.ar:11336/235881instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:52:46.669CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The regulation of proteins14-3-3 and the hippo pathway affect the adipogenesis or 3T3-L1
title The regulation of proteins14-3-3 and the hippo pathway affect the adipogenesis or 3T3-L1
spellingShingle The regulation of proteins14-3-3 and the hippo pathway affect the adipogenesis or 3T3-L1
del Veliz, Samanta
14-3-3
HIPPO PATHWAY
ADIPOGENESIS
GLP-1
title_short The regulation of proteins14-3-3 and the hippo pathway affect the adipogenesis or 3T3-L1
title_full The regulation of proteins14-3-3 and the hippo pathway affect the adipogenesis or 3T3-L1
title_fullStr The regulation of proteins14-3-3 and the hippo pathway affect the adipogenesis or 3T3-L1
title_full_unstemmed The regulation of proteins14-3-3 and the hippo pathway affect the adipogenesis or 3T3-L1
title_sort The regulation of proteins14-3-3 and the hippo pathway affect the adipogenesis or 3T3-L1
dc.creator.none.fl_str_mv del Veliz, Samanta
Uhart, Marina
Lim, Gareth
Bustos, Diego Martin
author del Veliz, Samanta
author_facet del Veliz, Samanta
Uhart, Marina
Lim, Gareth
Bustos, Diego Martin
author_role author
author2 Uhart, Marina
Lim, Gareth
Bustos, Diego Martin
author2_role author
author
author
dc.subject.none.fl_str_mv 14-3-3
HIPPO PATHWAY
ADIPOGENESIS
GLP-1
topic 14-3-3
HIPPO PATHWAY
ADIPOGENESIS
GLP-1
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Many transcription factors act sequentially to activate adipocyte differentiation. Multiple signal transduction pathways govern the adipocyte physiology. The Hippo kinase pathway is the main regulator of proliferation and differentiation of stem cells and adipocyte precursor cells. The 14-3-3 protein family, comprised of 7 paralogs in mammals, interacts with components of the Hippo kinase pathway regulating the adipogenic differentiation, although the specificity of these proteins in the process remains elusive. Deciphering the subcellular dynamics of 14-3-3 proteins will be instrumental in the discovery of new targets for the manipulation of stem cell fate decisions or treatment of chronic diseases, such as obesity and type 2 diabetes. In vitro adipocyte differentiation occurs by the addition of an Adipogenic Differentiation Medium (ADM) including Dulbecco's Modified Eagle Medium, 10% Fetal Bovine Serum, synthetic drugs (dexamethasone, IBMX, rosiglitazone) and peptide hormones (insulin). Since these mentioned chemical drugs are not present in physiological environments, it would be important to achieve a correct activated adipogenic program using fewer drugs and with natural origin. We have decided to evaluate the adipogenic potential of glucagon-like peptide 1 (GLP-1) analogs. In contrast to Native GLP-1 action, which is rapidly degraded by circulating Dipeptidyl Peptidase-4, GLP-1 analogs have shown positive effects on glycemic control and body weight due to their greater stability and longer half-life. In this project, experiments were performed on 3T3- L1 preadipocytes. First, the effect of different combinations of drugs for 7 days in adipogenesis was evaluated. Then, we carried out qPCR experiments to measure the gene expression of 14-3-3 and the most important proteins of the Hippo pathway, on days 3 and 7 of differentiation. We have determined that conditions resulting in greater adipogenic differentiation showed higher levels of Hippo pathway proteins and 14-3-3 gamma and beta isoforms on day 7. These effects were especially evident when IBMX was replaced by GLP-1 in the ADM. These results suggest that different inducers (glucocorticoids, thiazolidinediones, incretins), have different abilities for regulating the expression of 14-3-3 and hippo pathway proteins, thus affecting adipogenic differentiation. We are currently focused on elucidating the mechanisms of action of these drugs to have a greater understanding of the events that are necessary for adipogenesis to occur.
Fil: del Veliz, Samanta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Uhart, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Lim, Gareth.  University of Montreal Hospital Research Centre; Canadá
Fil: Bustos, Diego Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
LV Reunión Anual de la Sociedad Argentina de Investigaciones en Bioquímica; XIV Congreso de la Federación Panamericana de Bioquímica y Biología Molecular
Salta
Argentina
Sociedad Argentina de Investigaciones en Bioquímica
Federación Panamericana de Bioquímica y Biología Molecular
description Many transcription factors act sequentially to activate adipocyte differentiation. Multiple signal transduction pathways govern the adipocyte physiology. The Hippo kinase pathway is the main regulator of proliferation and differentiation of stem cells and adipocyte precursor cells. The 14-3-3 protein family, comprised of 7 paralogs in mammals, interacts with components of the Hippo kinase pathway regulating the adipogenic differentiation, although the specificity of these proteins in the process remains elusive. Deciphering the subcellular dynamics of 14-3-3 proteins will be instrumental in the discovery of new targets for the manipulation of stem cell fate decisions or treatment of chronic diseases, such as obesity and type 2 diabetes. In vitro adipocyte differentiation occurs by the addition of an Adipogenic Differentiation Medium (ADM) including Dulbecco's Modified Eagle Medium, 10% Fetal Bovine Serum, synthetic drugs (dexamethasone, IBMX, rosiglitazone) and peptide hormones (insulin). Since these mentioned chemical drugs are not present in physiological environments, it would be important to achieve a correct activated adipogenic program using fewer drugs and with natural origin. We have decided to evaluate the adipogenic potential of glucagon-like peptide 1 (GLP-1) analogs. In contrast to Native GLP-1 action, which is rapidly degraded by circulating Dipeptidyl Peptidase-4, GLP-1 analogs have shown positive effects on glycemic control and body weight due to their greater stability and longer half-life. In this project, experiments were performed on 3T3- L1 preadipocytes. First, the effect of different combinations of drugs for 7 days in adipogenesis was evaluated. Then, we carried out qPCR experiments to measure the gene expression of 14-3-3 and the most important proteins of the Hippo pathway, on days 3 and 7 of differentiation. We have determined that conditions resulting in greater adipogenic differentiation showed higher levels of Hippo pathway proteins and 14-3-3 gamma and beta isoforms on day 7. These effects were especially evident when IBMX was replaced by GLP-1 in the ADM. These results suggest that different inducers (glucocorticoids, thiazolidinediones, incretins), have different abilities for regulating the expression of 14-3-3 and hippo pathway proteins, thus affecting adipogenic differentiation. We are currently focused on elucidating the mechanisms of action of these drugs to have a greater understanding of the events that are necessary for adipogenesis to occur.
publishDate 2019
dc.date.none.fl_str_mv 2019
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dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/235881
The regulation of proteins14-3-3 and the hippo pathway affect the adipogenesis or 3T3-L1; LV Reunión Anual de la Sociedad Argentina de Investigaciones en Bioquímica; XIV Congreso de la Federación Panamericana de Bioquímica y Biología Molecular; Salta; Argentina; 2019; 52-52
0327-9545
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/235881
identifier_str_mv The regulation of proteins14-3-3 and the hippo pathway affect the adipogenesis or 3T3-L1; LV Reunión Anual de la Sociedad Argentina de Investigaciones en Bioquímica; XIV Congreso de la Federación Panamericana de Bioquímica y Biología Molecular; Salta; Argentina; 2019; 52-52
0327-9545
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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