New insights into Eragrostis curvula’s sexual and apomictic reproductive development

Autores
Pasten, Maria Cielo; Carballo, José; Diaz, Alejandra Raquel; Mizzotti, Chiara; Cucinotta, Mara; Colombo, Lucia; Echenique, Carmen Viviana; Mendes, Marta Adelina
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Apomixis, defined as asexual propagation by seeds, is considered of great importance for agriculture as it allows the fixation of desired traits and its propagation through generations. Eragrostis curvula (Schrad.) Ness, is a perennial grass that comprises a polymorphic complex including sexual and diplosporous apomictic cytotypes, where all apomicts are polyploids. In this study we present the first detailed description of female and male gametophyte development in E. curvula through confocal laser microscopy, contrasting three genotypes: the fully apomictic Tanganyika, the facultative apomictic Don Walter, and the sexual OTA-S. Moreover, we have studied the localized expression of a gene known as SQUAMOSA PROMOTER BINDING PROTEIN-LIKE7 (SPL7), that was found to be differentially expressed in contrasting genotypes of E. curvula. This gene had been previously linked with flower development and abiotic stresses in several species, thus, in situ hybridizations were carried out in the model plant Arabidopsis thaliana, as well as in sexual and apomictic E. curvula genotypes. Our microscopy analysis has led to the identification of specific morphological characteristics for each genotype, mainly depicting a larger ovule in the sexual genotype’s reproductive development after the meiosis stage. These results reveal potentially important features, which could be used for a simple identification of genotypes. Moreover, differential expression of the gene SPL7 was detected, specifically determining an overexpression of the gene in the sexual genotype. These results demonstrated that it could be an interesting candidate to understand the mechanisms behind apomictic development.
Fil: Pasten, Maria Cielo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
Fil: Carballo, José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Diaz, Alejandra Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Mizzotti, Chiara. Università degli Studi di Milano; Italia
Fil: Cucinotta, Mara. Università degli Studi di Milano; Italia
Fil: Colombo, Lucia. Università degli Studi di Milano; Italia
Fil: Echenique, Carmen Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
Fil: Mendes, Marta Adelina. Università degli Studi di Milano; Italia
Materia
Apomixis
Confocal laser microscopy
Eragrostis curvula
Ovule development
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/263265
Acceder
id CONICETDig_a3f681b465be1a48e792bdad48c93874
oai_identifier_str oai:ri.conicet.gov.ar:11336/263265
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling New insights into Eragrostis curvula’s sexual and apomictic reproductive developmentPasten, Maria CieloCarballo, JoséDiaz, Alejandra RaquelMizzotti, ChiaraCucinotta, MaraColombo, LuciaEchenique, Carmen VivianaMendes, Marta AdelinaApomixisConfocal laser microscopyEragrostis curvulaOvule developmenthttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Apomixis, defined as asexual propagation by seeds, is considered of great importance for agriculture as it allows the fixation of desired traits and its propagation through generations. Eragrostis curvula (Schrad.) Ness, is a perennial grass that comprises a polymorphic complex including sexual and diplosporous apomictic cytotypes, where all apomicts are polyploids. In this study we present the first detailed description of female and male gametophyte development in E. curvula through confocal laser microscopy, contrasting three genotypes: the fully apomictic Tanganyika, the facultative apomictic Don Walter, and the sexual OTA-S. Moreover, we have studied the localized expression of a gene known as SQUAMOSA PROMOTER BINDING PROTEIN-LIKE7 (SPL7), that was found to be differentially expressed in contrasting genotypes of E. curvula. This gene had been previously linked with flower development and abiotic stresses in several species, thus, in situ hybridizations were carried out in the model plant Arabidopsis thaliana, as well as in sexual and apomictic E. curvula genotypes. Our microscopy analysis has led to the identification of specific morphological characteristics for each genotype, mainly depicting a larger ovule in the sexual genotype’s reproductive development after the meiosis stage. These results reveal potentially important features, which could be used for a simple identification of genotypes. Moreover, differential expression of the gene SPL7 was detected, specifically determining an overexpression of the gene in the sexual genotype. These results demonstrated that it could be an interesting candidate to understand the mechanisms behind apomictic development.Fil: Pasten, Maria Cielo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaFil: Carballo, José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Diaz, Alejandra Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Mizzotti, Chiara. Università degli Studi di Milano; ItaliaFil: Cucinotta, Mara. Università degli Studi di Milano; ItaliaFil: Colombo, Lucia. Università degli Studi di Milano; ItaliaFil: Echenique, Carmen Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaFil: Mendes, Marta Adelina. Università degli Studi di Milano; ItaliaFrontiers Media2025-05-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/263265Pasten, Maria Cielo; Carballo, José; Diaz, Alejandra Raquel; Mizzotti, Chiara; Cucinotta, Mara; et al.; New insights into Eragrostis curvula’s sexual and apomictic reproductive development; Frontiers Media; Frontiers in Plant Science; 16; 1-5-2025; 1-151664-462XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fpls.2025.1530855/fullinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fpls.2025.1530855info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:54:03Zoai:ri.conicet.gov.ar:11336/263265instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:54:03.375CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv New insights into Eragrostis curvula’s sexual and apomictic reproductive development
title New insights into Eragrostis curvula’s sexual and apomictic reproductive development
spellingShingle New insights into Eragrostis curvula’s sexual and apomictic reproductive development
Pasten, Maria Cielo
Apomixis
Confocal laser microscopy
Eragrostis curvula
Ovule development
title_short New insights into Eragrostis curvula’s sexual and apomictic reproductive development
title_full New insights into Eragrostis curvula’s sexual and apomictic reproductive development
title_fullStr New insights into Eragrostis curvula’s sexual and apomictic reproductive development
title_full_unstemmed New insights into Eragrostis curvula’s sexual and apomictic reproductive development
title_sort New insights into Eragrostis curvula’s sexual and apomictic reproductive development
dc.creator.none.fl_str_mv Pasten, Maria Cielo
Carballo, José
Diaz, Alejandra Raquel
Mizzotti, Chiara
Cucinotta, Mara
Colombo, Lucia
Echenique, Carmen Viviana
Mendes, Marta Adelina
author Pasten, Maria Cielo
author_facet Pasten, Maria Cielo
Carballo, José
Diaz, Alejandra Raquel
Mizzotti, Chiara
Cucinotta, Mara
Colombo, Lucia
Echenique, Carmen Viviana
Mendes, Marta Adelina
author_role author
author2 Carballo, José
Diaz, Alejandra Raquel
Mizzotti, Chiara
Cucinotta, Mara
Colombo, Lucia
Echenique, Carmen Viviana
Mendes, Marta Adelina
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Apomixis
Confocal laser microscopy
Eragrostis curvula
Ovule development
topic Apomixis
Confocal laser microscopy
Eragrostis curvula
Ovule development
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Apomixis, defined as asexual propagation by seeds, is considered of great importance for agriculture as it allows the fixation of desired traits and its propagation through generations. Eragrostis curvula (Schrad.) Ness, is a perennial grass that comprises a polymorphic complex including sexual and diplosporous apomictic cytotypes, where all apomicts are polyploids. In this study we present the first detailed description of female and male gametophyte development in E. curvula through confocal laser microscopy, contrasting three genotypes: the fully apomictic Tanganyika, the facultative apomictic Don Walter, and the sexual OTA-S. Moreover, we have studied the localized expression of a gene known as SQUAMOSA PROMOTER BINDING PROTEIN-LIKE7 (SPL7), that was found to be differentially expressed in contrasting genotypes of E. curvula. This gene had been previously linked with flower development and abiotic stresses in several species, thus, in situ hybridizations were carried out in the model plant Arabidopsis thaliana, as well as in sexual and apomictic E. curvula genotypes. Our microscopy analysis has led to the identification of specific morphological characteristics for each genotype, mainly depicting a larger ovule in the sexual genotype’s reproductive development after the meiosis stage. These results reveal potentially important features, which could be used for a simple identification of genotypes. Moreover, differential expression of the gene SPL7 was detected, specifically determining an overexpression of the gene in the sexual genotype. These results demonstrated that it could be an interesting candidate to understand the mechanisms behind apomictic development.
Fil: Pasten, Maria Cielo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
Fil: Carballo, José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Diaz, Alejandra Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Mizzotti, Chiara. Università degli Studi di Milano; Italia
Fil: Cucinotta, Mara. Università degli Studi di Milano; Italia
Fil: Colombo, Lucia. Università degli Studi di Milano; Italia
Fil: Echenique, Carmen Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
Fil: Mendes, Marta Adelina. Università degli Studi di Milano; Italia
description Apomixis, defined as asexual propagation by seeds, is considered of great importance for agriculture as it allows the fixation of desired traits and its propagation through generations. Eragrostis curvula (Schrad.) Ness, is a perennial grass that comprises a polymorphic complex including sexual and diplosporous apomictic cytotypes, where all apomicts are polyploids. In this study we present the first detailed description of female and male gametophyte development in E. curvula through confocal laser microscopy, contrasting three genotypes: the fully apomictic Tanganyika, the facultative apomictic Don Walter, and the sexual OTA-S. Moreover, we have studied the localized expression of a gene known as SQUAMOSA PROMOTER BINDING PROTEIN-LIKE7 (SPL7), that was found to be differentially expressed in contrasting genotypes of E. curvula. This gene had been previously linked with flower development and abiotic stresses in several species, thus, in situ hybridizations were carried out in the model plant Arabidopsis thaliana, as well as in sexual and apomictic E. curvula genotypes. Our microscopy analysis has led to the identification of specific morphological characteristics for each genotype, mainly depicting a larger ovule in the sexual genotype’s reproductive development after the meiosis stage. These results reveal potentially important features, which could be used for a simple identification of genotypes. Moreover, differential expression of the gene SPL7 was detected, specifically determining an overexpression of the gene in the sexual genotype. These results demonstrated that it could be an interesting candidate to understand the mechanisms behind apomictic development.
publishDate 2025
dc.date.none.fl_str_mv 2025-05-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/263265
Pasten, Maria Cielo; Carballo, José; Diaz, Alejandra Raquel; Mizzotti, Chiara; Cucinotta, Mara; et al.; New insights into Eragrostis curvula’s sexual and apomictic reproductive development; Frontiers Media; Frontiers in Plant Science; 16; 1-5-2025; 1-15
1664-462X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/263265
identifier_str_mv Pasten, Maria Cielo; Carballo, José; Diaz, Alejandra Raquel; Mizzotti, Chiara; Cucinotta, Mara; et al.; New insights into Eragrostis curvula’s sexual and apomictic reproductive development; Frontiers Media; Frontiers in Plant Science; 16; 1-5-2025; 1-15
1664-462X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fpls.2025.1530855/full
info:eu-repo/semantics/altIdentifier/doi/10.3389/fpls.2025.1530855
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269261911818240
score 13.13397

Publicaciones similares