A reproductive calendar of apomictic and sexual genotypes of Eragrostis curvula (Schrad.) Ness

Autores
Bisp, Petrus; Selva, Juan Pablo; Carballo, José; Zappacosta, Diego Carlos; Gallardo, Jimena Alicia; Echenique, Carmen Viviana
Año de publicación
2022
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Weeping lovegrass (Eragrostis curvula) is a perennial forage grass nativeto southern Africa naturalized in semiarid regions of Argentina thatreproduces mainly by apomixis, showing full, facultative and sexualgenotypes. The type of apomixis present in E. curvula is diplospory,where the megaspore mother cell (MMC) undergoes mitosis instead ofmeiosis and there is no fertilization of the egg cell, only the polar nucleusis fertilized to form the endosperm. To study the genetic basis of thedifferent components of apomixis in this grass, it is important to be ableto recognize and isolate pistils at different developmental stages in orderto identify differentially expressed genes. The aim of this work was todevelop a detailed and precise reproductive calendar for its use intranscriptomic analysis. The methyl salicylate clarification method wasused in order to avoid the long process of paraffin embedding protocol,and the male and female developmental stages in anthers and pistilswere confirmed through Differential Interference Contrast microscopy.Five different growth parameters from pistils and anthers from differentgenotypes were registered during the mega and micro-sporogenesis andgametogenesis over more than 500 flowers. Pistil length was found easyto measure and effective to identify different developmental stages. Thisparameter was more reliable than anther length to infer the correct femaledevelopmental stage, providing an effective method to collect pistils atthe desired stage.
Fil: Bisp, Petrus. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Selva, Juan Pablo. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Carballo, José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Zappacosta, Diego Carlos. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Gallardo, Jimena Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
Fil: Echenique, Carmen Viviana. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
26th International Conference on Sexual Plant Reproduction
Praga
República Checa
Institute of Experimental Botany
Materia
ERAGROSTIS CURVULA
APOMIXIS
EMBRYO SAC
REPRODUCTIVE CALENDAR
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/221588
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A reproductive calendar of apomictic and sexual genotypes of Eragrostis curvula (Schrad.) NessBisp, PetrusSelva, Juan PabloCarballo, JoséZappacosta, Diego CarlosGallardo, Jimena AliciaEchenique, Carmen VivianaERAGROSTIS CURVULAAPOMIXISEMBRYO SACREPRODUCTIVE CALENDARhttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Weeping lovegrass (Eragrostis curvula) is a perennial forage grass nativeto southern Africa naturalized in semiarid regions of Argentina thatreproduces mainly by apomixis, showing full, facultative and sexualgenotypes. The type of apomixis present in E. curvula is diplospory,where the megaspore mother cell (MMC) undergoes mitosis instead ofmeiosis and there is no fertilization of the egg cell, only the polar nucleusis fertilized to form the endosperm. To study the genetic basis of thedifferent components of apomixis in this grass, it is important to be ableto recognize and isolate pistils at different developmental stages in orderto identify differentially expressed genes. The aim of this work was todevelop a detailed and precise reproductive calendar for its use intranscriptomic analysis. The methyl salicylate clarification method wasused in order to avoid the long process of paraffin embedding protocol,and the male and female developmental stages in anthers and pistilswere confirmed through Differential Interference Contrast microscopy.Five different growth parameters from pistils and anthers from differentgenotypes were registered during the mega and micro-sporogenesis andgametogenesis over more than 500 flowers. Pistil length was found easyto measure and effective to identify different developmental stages. Thisparameter was more reliable than anther length to infer the correct femaledevelopmental stage, providing an effective method to collect pistils atthe desired stage.Fil: Bisp, Petrus. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Selva, Juan Pablo. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Carballo, José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Zappacosta, Diego Carlos. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Gallardo, Jimena Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaFil: Echenique, Carmen Viviana. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina26th International Conference on Sexual Plant ReproductionPragaRepública ChecaInstitute of Experimental BotanyInstitute of Experimental Botany2022info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectConferenciaBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/221588A reproductive calendar of apomictic and sexual genotypes of Eragrostis curvula (Schrad.) Ness; 26th International Conference on Sexual Plant Reproduction; Praga; República Checa; 2022; 148-148CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.prague2020.eu/resources/Abstract-Book_ICSPR_18.06.2022.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:53:38Zoai:ri.conicet.gov.ar:11336/221588instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:53:39.141CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A reproductive calendar of apomictic and sexual genotypes of Eragrostis curvula (Schrad.) Ness
title A reproductive calendar of apomictic and sexual genotypes of Eragrostis curvula (Schrad.) Ness
spellingShingle A reproductive calendar of apomictic and sexual genotypes of Eragrostis curvula (Schrad.) Ness
Bisp, Petrus
ERAGROSTIS CURVULA
APOMIXIS
EMBRYO SAC
REPRODUCTIVE CALENDAR
title_short A reproductive calendar of apomictic and sexual genotypes of Eragrostis curvula (Schrad.) Ness
title_full A reproductive calendar of apomictic and sexual genotypes of Eragrostis curvula (Schrad.) Ness
title_fullStr A reproductive calendar of apomictic and sexual genotypes of Eragrostis curvula (Schrad.) Ness
title_full_unstemmed A reproductive calendar of apomictic and sexual genotypes of Eragrostis curvula (Schrad.) Ness
title_sort A reproductive calendar of apomictic and sexual genotypes of Eragrostis curvula (Schrad.) Ness
dc.creator.none.fl_str_mv Bisp, Petrus
Selva, Juan Pablo
Carballo, José
Zappacosta, Diego Carlos
Gallardo, Jimena Alicia
Echenique, Carmen Viviana
author Bisp, Petrus
author_facet Bisp, Petrus
Selva, Juan Pablo
Carballo, José
Zappacosta, Diego Carlos
Gallardo, Jimena Alicia
Echenique, Carmen Viviana
author_role author
author2 Selva, Juan Pablo
Carballo, José
Zappacosta, Diego Carlos
Gallardo, Jimena Alicia
Echenique, Carmen Viviana
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv ERAGROSTIS CURVULA
APOMIXIS
EMBRYO SAC
REPRODUCTIVE CALENDAR
topic ERAGROSTIS CURVULA
APOMIXIS
EMBRYO SAC
REPRODUCTIVE CALENDAR
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Weeping lovegrass (Eragrostis curvula) is a perennial forage grass nativeto southern Africa naturalized in semiarid regions of Argentina thatreproduces mainly by apomixis, showing full, facultative and sexualgenotypes. The type of apomixis present in E. curvula is diplospory,where the megaspore mother cell (MMC) undergoes mitosis instead ofmeiosis and there is no fertilization of the egg cell, only the polar nucleusis fertilized to form the endosperm. To study the genetic basis of thedifferent components of apomixis in this grass, it is important to be ableto recognize and isolate pistils at different developmental stages in orderto identify differentially expressed genes. The aim of this work was todevelop a detailed and precise reproductive calendar for its use intranscriptomic analysis. The methyl salicylate clarification method wasused in order to avoid the long process of paraffin embedding protocol,and the male and female developmental stages in anthers and pistilswere confirmed through Differential Interference Contrast microscopy.Five different growth parameters from pistils and anthers from differentgenotypes were registered during the mega and micro-sporogenesis andgametogenesis over more than 500 flowers. Pistil length was found easyto measure and effective to identify different developmental stages. Thisparameter was more reliable than anther length to infer the correct femaledevelopmental stage, providing an effective method to collect pistils atthe desired stage.
Fil: Bisp, Petrus. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Selva, Juan Pablo. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Carballo, José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Zappacosta, Diego Carlos. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Gallardo, Jimena Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
Fil: Echenique, Carmen Viviana. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
26th International Conference on Sexual Plant Reproduction
Praga
República Checa
Institute of Experimental Botany
description Weeping lovegrass (Eragrostis curvula) is a perennial forage grass nativeto southern Africa naturalized in semiarid regions of Argentina thatreproduces mainly by apomixis, showing full, facultative and sexualgenotypes. The type of apomixis present in E. curvula is diplospory,where the megaspore mother cell (MMC) undergoes mitosis instead ofmeiosis and there is no fertilization of the egg cell, only the polar nucleusis fertilized to form the endosperm. To study the genetic basis of thedifferent components of apomixis in this grass, it is important to be ableto recognize and isolate pistils at different developmental stages in orderto identify differentially expressed genes. The aim of this work was todevelop a detailed and precise reproductive calendar for its use intranscriptomic analysis. The methyl salicylate clarification method wasused in order to avoid the long process of paraffin embedding protocol,and the male and female developmental stages in anthers and pistilswere confirmed through Differential Interference Contrast microscopy.Five different growth parameters from pistils and anthers from differentgenotypes were registered during the mega and micro-sporogenesis andgametogenesis over more than 500 flowers. Pistil length was found easyto measure and effective to identify different developmental stages. Thisparameter was more reliable than anther length to infer the correct femaledevelopmental stage, providing an effective method to collect pistils atthe desired stage.
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Conferencia
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/221588
A reproductive calendar of apomictic and sexual genotypes of Eragrostis curvula (Schrad.) Ness; 26th International Conference on Sexual Plant Reproduction; Praga; República Checa; 2022; 148-148
CONICET Digital
CONICET
url http://hdl.handle.net/11336/221588
identifier_str_mv A reproductive calendar of apomictic and sexual genotypes of Eragrostis curvula (Schrad.) Ness; 26th International Conference on Sexual Plant Reproduction; Praga; República Checa; 2022; 148-148
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.prague2020.eu/resources/Abstract-Book_ICSPR_18.06.2022.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Institute of Experimental Botany
publisher.none.fl_str_mv Institute of Experimental Botany
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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