A 3D analysis of the reproductive development of Eragrostis curvula (Schrad.) Ness

Autores
Pasten, Maria Cielo; Carballo, José; Diaz, Alejandra Raquel; Mizzotti, Chiara; Cucinotta, Mara; Colombo, Lucia; Echenique, Carmen Viviana; Mendes, Marta Adelina
Año de publicación
2023
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Apomixis, defined as asexual propagation by seeds, is a type of plant reproduction found in more than 400angiosperms. This polyphyletic trait has been studied in various species, and it is considered of greatimportance in the agricultural industry as it would allow the fixation of desired traits and its propagationthrough generations. The study of ovule development in an apomictic model is necessary to strengthen theknowledge of the mechanisms governing this reproductive mode, and would contribute to improve plantbreeding procedures. Weeping lovegrass (Eragrostis curvula (Schrad.) Ness) is a perennial grass that has beenextensively studied as a model species for diplosporous apomixis. This species, mostly used as forage insemi-arid regions of the world, comprises a polymorphic complex that includes sexual and apomicticcytotypes, where all apomicts are polyploids, ranging from tetraploids (4X) to octoploids (8X). The aim of thiswork was to provide a thorough description of the developmental stages taking place in the ovule of threetetraploid genotypes of weeping lovegrass: the full apomictic Tanganyika, the facultative apomictic DonWalter, and the sexual OTA, as well as evaluating pollen development, using confocal laser microscopy.Moreover, in order to further understand the mode of reproduction in this species, anin-situ hybridization (ISH)was performed using an SPL gene (Squamosa Promoter-binding-Like), found to be differentially expressedbetween two of the contrasting genotypes (OTA-S and Tanganyika). The present analysis of the femalegametophyte aided in increasing the knowledge of the reproductive development in E. curvula and allowed theidentification of differences between sexual and apomictic development.
Fil: Pasten, Maria Cielo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
Fil: Carballo, José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Diaz, Alejandra Raquel. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Mizzotti, Chiara. Università degli Studi di Milano; Italia
Fil: Cucinotta, Mara. Università degli Studi di Milano; Italia
Fil: Colombo, Lucia. Università degli Studi di Milano; Italia
Fil: Echenique, Carmen Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
Fil: Mendes, Marta Adelina. Università degli Studi di Milano; Italia
IV International Congress on Apomixis
Rosario
Argentina
Instituto de Investigaciones en Ciencias Agrarias de Rosario
Universidad Nacional de Rosario
Materia
Apomixis
Eragrostis curvula
Confocal laser microscopy
In situ hibridization
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/224360
Acceder
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spelling A 3D analysis of the reproductive development of Eragrostis curvula (Schrad.) NessPasten, Maria CieloCarballo, JoséDiaz, Alejandra RaquelMizzotti, ChiaraCucinotta, MaraColombo, LuciaEchenique, Carmen VivianaMendes, Marta AdelinaApomixisEragrostis curvulaConfocal laser microscopyIn situ hibridizationhttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Apomixis, defined as asexual propagation by seeds, is a type of plant reproduction found in more than 400angiosperms. This polyphyletic trait has been studied in various species, and it is considered of greatimportance in the agricultural industry as it would allow the fixation of desired traits and its propagationthrough generations. The study of ovule development in an apomictic model is necessary to strengthen theknowledge of the mechanisms governing this reproductive mode, and would contribute to improve plantbreeding procedures. Weeping lovegrass (Eragrostis curvula (Schrad.) Ness) is a perennial grass that has beenextensively studied as a model species for diplosporous apomixis. This species, mostly used as forage insemi-arid regions of the world, comprises a polymorphic complex that includes sexual and apomicticcytotypes, where all apomicts are polyploids, ranging from tetraploids (4X) to octoploids (8X). The aim of thiswork was to provide a thorough description of the developmental stages taking place in the ovule of threetetraploid genotypes of weeping lovegrass: the full apomictic Tanganyika, the facultative apomictic DonWalter, and the sexual OTA, as well as evaluating pollen development, using confocal laser microscopy.Moreover, in order to further understand the mode of reproduction in this species, anin-situ hybridization (ISH)was performed using an SPL gene (Squamosa Promoter-binding-Like), found to be differentially expressedbetween two of the contrasting genotypes (OTA-S and Tanganyika). The present analysis of the femalegametophyte aided in increasing the knowledge of the reproductive development in E. curvula and allowed theidentification of differences between sexual and apomictic development.Fil: Pasten, Maria Cielo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaFil: Carballo, José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Diaz, Alejandra Raquel. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Mizzotti, Chiara. Università degli Studi di Milano; ItaliaFil: Cucinotta, Mara. Università degli Studi di Milano; ItaliaFil: Colombo, Lucia. Università degli Studi di Milano; ItaliaFil: Echenique, Carmen Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaFil: Mendes, Marta Adelina. Università degli Studi di Milano; ItaliaIV International Congress on ApomixisRosarioArgentinaInstituto de Investigaciones en Ciencias Agrarias de RosarioUniversidad Nacional de RosarioIV International Congress on Apomixis2023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/224360A 3D analysis of the reproductive development of Eragrostis curvula (Schrad.) Ness; IV International Congress on Apomixis; Rosario; Argentina; 2023; 50-50CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://apomixis2023.com.ar/wp-content/uploads/APOMIXIS-2023-book-of-abstracts.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:07:23Zoai:ri.conicet.gov.ar:11336/224360instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:07:23.276CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A 3D analysis of the reproductive development of Eragrostis curvula (Schrad.) Ness
title A 3D analysis of the reproductive development of Eragrostis curvula (Schrad.) Ness
spellingShingle A 3D analysis of the reproductive development of Eragrostis curvula (Schrad.) Ness
Pasten, Maria Cielo
Apomixis
Eragrostis curvula
Confocal laser microscopy
In situ hibridization
title_short A 3D analysis of the reproductive development of Eragrostis curvula (Schrad.) Ness
title_full A 3D analysis of the reproductive development of Eragrostis curvula (Schrad.) Ness
title_fullStr A 3D analysis of the reproductive development of Eragrostis curvula (Schrad.) Ness
title_full_unstemmed A 3D analysis of the reproductive development of Eragrostis curvula (Schrad.) Ness
title_sort A 3D analysis of the reproductive development of Eragrostis curvula (Schrad.) Ness
dc.creator.none.fl_str_mv Pasten, Maria Cielo
Carballo, José
Diaz, Alejandra Raquel
Mizzotti, Chiara
Cucinotta, Mara
Colombo, Lucia
Echenique, Carmen Viviana
Mendes, Marta Adelina
author Pasten, Maria Cielo
author_facet Pasten, Maria Cielo
Carballo, José
Diaz, Alejandra Raquel
Mizzotti, Chiara
Cucinotta, Mara
Colombo, Lucia
Echenique, Carmen Viviana
Mendes, Marta Adelina
author_role author
author2 Carballo, José
Diaz, Alejandra Raquel
Mizzotti, Chiara
Cucinotta, Mara
Colombo, Lucia
Echenique, Carmen Viviana
Mendes, Marta Adelina
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Apomixis
Eragrostis curvula
Confocal laser microscopy
In situ hibridization
topic Apomixis
Eragrostis curvula
Confocal laser microscopy
In situ hibridization
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Apomixis, defined as asexual propagation by seeds, is a type of plant reproduction found in more than 400angiosperms. This polyphyletic trait has been studied in various species, and it is considered of greatimportance in the agricultural industry as it would allow the fixation of desired traits and its propagationthrough generations. The study of ovule development in an apomictic model is necessary to strengthen theknowledge of the mechanisms governing this reproductive mode, and would contribute to improve plantbreeding procedures. Weeping lovegrass (Eragrostis curvula (Schrad.) Ness) is a perennial grass that has beenextensively studied as a model species for diplosporous apomixis. This species, mostly used as forage insemi-arid regions of the world, comprises a polymorphic complex that includes sexual and apomicticcytotypes, where all apomicts are polyploids, ranging from tetraploids (4X) to octoploids (8X). The aim of thiswork was to provide a thorough description of the developmental stages taking place in the ovule of threetetraploid genotypes of weeping lovegrass: the full apomictic Tanganyika, the facultative apomictic DonWalter, and the sexual OTA, as well as evaluating pollen development, using confocal laser microscopy.Moreover, in order to further understand the mode of reproduction in this species, anin-situ hybridization (ISH)was performed using an SPL gene (Squamosa Promoter-binding-Like), found to be differentially expressedbetween two of the contrasting genotypes (OTA-S and Tanganyika). The present analysis of the femalegametophyte aided in increasing the knowledge of the reproductive development in E. curvula and allowed theidentification of differences between sexual and apomictic development.
Fil: Pasten, Maria Cielo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
Fil: Carballo, José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Diaz, Alejandra Raquel. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Mizzotti, Chiara. Università degli Studi di Milano; Italia
Fil: Cucinotta, Mara. Università degli Studi di Milano; Italia
Fil: Colombo, Lucia. Università degli Studi di Milano; Italia
Fil: Echenique, Carmen Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
Fil: Mendes, Marta Adelina. Università degli Studi di Milano; Italia
IV International Congress on Apomixis
Rosario
Argentina
Instituto de Investigaciones en Ciencias Agrarias de Rosario
Universidad Nacional de Rosario
description Apomixis, defined as asexual propagation by seeds, is a type of plant reproduction found in more than 400angiosperms. This polyphyletic trait has been studied in various species, and it is considered of greatimportance in the agricultural industry as it would allow the fixation of desired traits and its propagationthrough generations. The study of ovule development in an apomictic model is necessary to strengthen theknowledge of the mechanisms governing this reproductive mode, and would contribute to improve plantbreeding procedures. Weeping lovegrass (Eragrostis curvula (Schrad.) Ness) is a perennial grass that has beenextensively studied as a model species for diplosporous apomixis. This species, mostly used as forage insemi-arid regions of the world, comprises a polymorphic complex that includes sexual and apomicticcytotypes, where all apomicts are polyploids, ranging from tetraploids (4X) to octoploids (8X). The aim of thiswork was to provide a thorough description of the developmental stages taking place in the ovule of threetetraploid genotypes of weeping lovegrass: the full apomictic Tanganyika, the facultative apomictic DonWalter, and the sexual OTA, as well as evaluating pollen development, using confocal laser microscopy.Moreover, in order to further understand the mode of reproduction in this species, anin-situ hybridization (ISH)was performed using an SPL gene (Squamosa Promoter-binding-Like), found to be differentially expressedbetween two of the contrasting genotypes (OTA-S and Tanganyika). The present analysis of the femalegametophyte aided in increasing the knowledge of the reproductive development in E. curvula and allowed theidentification of differences between sexual and apomictic development.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/224360
A 3D analysis of the reproductive development of Eragrostis curvula (Schrad.) Ness; IV International Congress on Apomixis; Rosario; Argentina; 2023; 50-50
CONICET Digital
CONICET
url http://hdl.handle.net/11336/224360
identifier_str_mv A 3D analysis of the reproductive development of Eragrostis curvula (Schrad.) Ness; IV International Congress on Apomixis; Rosario; Argentina; 2023; 50-50
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://apomixis2023.com.ar/wp-content/uploads/APOMIXIS-2023-book-of-abstracts.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv IV International Congress on Apomixis
publisher.none.fl_str_mv IV International Congress on Apomixis
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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