Gamma oscillations in the pedunculopontine nucleus are regulated by F-actin: neuroepigenetic implications

Autores
Urbano Suarez, Francisco Jose; Bisagno, Veronica; Garcia Rill, Edgar
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The pedunculopontine nucleus (PPN) is part of the reticular activating system (RAS) in charge of arousal and rapid eye movement sleep. The presence of high-frequency membrane oscillations in the gamma-band range in the PPN has been extensively demonstrated both in vivo and in vitro. Our group previously described histone deacetylation (HDAC) inhibition in vitro induced protein changes in F-actin cytoskeleton and intracellular Ca2+ concentration regulation proteins in the PPN. Here, we present evidence that supports the presence of a fine balance between HDAC function and calcium calmodulin kinase II-F-actin interactions in the PPN. We modified F-actin polymerization in vitro by using jasplakinolide (1 μM, a promoter of F-actin stabilization), or latrunculin-B (1 μM, an inhibitor of actin polymerization). Our results showed that shifting the balance in either direction significantly reduced PPN gamma oscillation as well as voltage-dependent calcium currents.
Fil: Urbano Suarez, Francisco Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones Farmacológicas; Argentina
Fil: Bisagno, Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Garcia Rill, Edgar. University of Arkansas for Medical Sciences; Estados Unidos
Materia
AROUSAL
CALCIUM CHANNELS
CALCIUM CURRENTS
GAMMA OSCILLATIONS
HISTONE DEACETYLATION
NEUROEPIGENETICS
TRICHOSTATIN A
WAKING
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/139129
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/139129
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Gamma oscillations in the pedunculopontine nucleus are regulated by F-actin: neuroepigenetic implicationsUrbano Suarez, Francisco JoseBisagno, VeronicaGarcia Rill, EdgarAROUSALCALCIUM CHANNELSCALCIUM CURRENTSGAMMA OSCILLATIONSHISTONE DEACETYLATIONNEUROEPIGENETICSTRICHOSTATIN AWAKINGhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The pedunculopontine nucleus (PPN) is part of the reticular activating system (RAS) in charge of arousal and rapid eye movement sleep. The presence of high-frequency membrane oscillations in the gamma-band range in the PPN has been extensively demonstrated both in vivo and in vitro. Our group previously described histone deacetylation (HDAC) inhibition in vitro induced protein changes in F-actin cytoskeleton and intracellular Ca2+ concentration regulation proteins in the PPN. Here, we present evidence that supports the presence of a fine balance between HDAC function and calcium calmodulin kinase II-F-actin interactions in the PPN. We modified F-actin polymerization in vitro by using jasplakinolide (1 μM, a promoter of F-actin stabilization), or latrunculin-B (1 μM, an inhibitor of actin polymerization). Our results showed that shifting the balance in either direction significantly reduced PPN gamma oscillation as well as voltage-dependent calcium currents.Fil: Urbano Suarez, Francisco Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones Farmacológicas; ArgentinaFil: Bisagno, Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; ArgentinaFil: Garcia Rill, Edgar. University of Arkansas for Medical Sciences; Estados UnidosAmerican Physiological Society2020-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/139129Urbano Suarez, Francisco Jose; Bisagno, Veronica; Garcia Rill, Edgar; Gamma oscillations in the pedunculopontine nucleus are regulated by F-actin: neuroepigenetic implications; American Physiological Society; American Journal of Physiology-cell Physiology; 318; 2; 2-2020; C282-C2880363-6143CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.physiology.org/doi/10.1152/ajpcell.00374.2019info:eu-repo/semantics/altIdentifier/doi/10.1152/ajpcell.00374.2019info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:09:27Zoai:ri.conicet.gov.ar:11336/139129instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:09:27.583CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Gamma oscillations in the pedunculopontine nucleus are regulated by F-actin: neuroepigenetic implications
title Gamma oscillations in the pedunculopontine nucleus are regulated by F-actin: neuroepigenetic implications
spellingShingle Gamma oscillations in the pedunculopontine nucleus are regulated by F-actin: neuroepigenetic implications
Urbano Suarez, Francisco Jose
AROUSAL
CALCIUM CHANNELS
CALCIUM CURRENTS
GAMMA OSCILLATIONS
HISTONE DEACETYLATION
NEUROEPIGENETICS
TRICHOSTATIN A
WAKING
title_short Gamma oscillations in the pedunculopontine nucleus are regulated by F-actin: neuroepigenetic implications
title_full Gamma oscillations in the pedunculopontine nucleus are regulated by F-actin: neuroepigenetic implications
title_fullStr Gamma oscillations in the pedunculopontine nucleus are regulated by F-actin: neuroepigenetic implications
title_full_unstemmed Gamma oscillations in the pedunculopontine nucleus are regulated by F-actin: neuroepigenetic implications
title_sort Gamma oscillations in the pedunculopontine nucleus are regulated by F-actin: neuroepigenetic implications
dc.creator.none.fl_str_mv Urbano Suarez, Francisco Jose
Bisagno, Veronica
Garcia Rill, Edgar
author Urbano Suarez, Francisco Jose
author_facet Urbano Suarez, Francisco Jose
Bisagno, Veronica
Garcia Rill, Edgar
author_role author
author2 Bisagno, Veronica
Garcia Rill, Edgar
author2_role author
author
dc.subject.none.fl_str_mv AROUSAL
CALCIUM CHANNELS
CALCIUM CURRENTS
GAMMA OSCILLATIONS
HISTONE DEACETYLATION
NEUROEPIGENETICS
TRICHOSTATIN A
WAKING
topic AROUSAL
CALCIUM CHANNELS
CALCIUM CURRENTS
GAMMA OSCILLATIONS
HISTONE DEACETYLATION
NEUROEPIGENETICS
TRICHOSTATIN A
WAKING
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The pedunculopontine nucleus (PPN) is part of the reticular activating system (RAS) in charge of arousal and rapid eye movement sleep. The presence of high-frequency membrane oscillations in the gamma-band range in the PPN has been extensively demonstrated both in vivo and in vitro. Our group previously described histone deacetylation (HDAC) inhibition in vitro induced protein changes in F-actin cytoskeleton and intracellular Ca2+ concentration regulation proteins in the PPN. Here, we present evidence that supports the presence of a fine balance between HDAC function and calcium calmodulin kinase II-F-actin interactions in the PPN. We modified F-actin polymerization in vitro by using jasplakinolide (1 μM, a promoter of F-actin stabilization), or latrunculin-B (1 μM, an inhibitor of actin polymerization). Our results showed that shifting the balance in either direction significantly reduced PPN gamma oscillation as well as voltage-dependent calcium currents.
Fil: Urbano Suarez, Francisco Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones Farmacológicas; Argentina
Fil: Bisagno, Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Garcia Rill, Edgar. University of Arkansas for Medical Sciences; Estados Unidos
description The pedunculopontine nucleus (PPN) is part of the reticular activating system (RAS) in charge of arousal and rapid eye movement sleep. The presence of high-frequency membrane oscillations in the gamma-band range in the PPN has been extensively demonstrated both in vivo and in vitro. Our group previously described histone deacetylation (HDAC) inhibition in vitro induced protein changes in F-actin cytoskeleton and intracellular Ca2+ concentration regulation proteins in the PPN. Here, we present evidence that supports the presence of a fine balance between HDAC function and calcium calmodulin kinase II-F-actin interactions in the PPN. We modified F-actin polymerization in vitro by using jasplakinolide (1 μM, a promoter of F-actin stabilization), or latrunculin-B (1 μM, an inhibitor of actin polymerization). Our results showed that shifting the balance in either direction significantly reduced PPN gamma oscillation as well as voltage-dependent calcium currents.
publishDate 2020
dc.date.none.fl_str_mv 2020-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/139129
Urbano Suarez, Francisco Jose; Bisagno, Veronica; Garcia Rill, Edgar; Gamma oscillations in the pedunculopontine nucleus are regulated by F-actin: neuroepigenetic implications; American Physiological Society; American Journal of Physiology-cell Physiology; 318; 2; 2-2020; C282-C288
0363-6143
CONICET Digital
CONICET
url http://hdl.handle.net/11336/139129
identifier_str_mv Urbano Suarez, Francisco Jose; Bisagno, Veronica; Garcia Rill, Edgar; Gamma oscillations in the pedunculopontine nucleus are regulated by F-actin: neuroepigenetic implications; American Physiological Society; American Journal of Physiology-cell Physiology; 318; 2; 2-2020; C282-C288
0363-6143
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.physiology.org/doi/10.1152/ajpcell.00374.2019
info:eu-repo/semantics/altIdentifier/doi/10.1152/ajpcell.00374.2019
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Physiological Society
publisher.none.fl_str_mv American Physiological Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846782472185970688
score 12.982451

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