Sublethal and lethal effects on Rhinella Arenarum (Anura, Bufonidae) tadpoles exerted by the pirimicarb-containing technical formulation insecticide Aficida®

Autores
Vera Candioti, Josefina; Natale, Guillermo Sebastian; Soloneski, Sonia Maria Elsa; Ronco, Alicia Estela; Larramendy, Marcelo Luis
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Acute toxicity, genotoxicity, and cytotoxicity of the pirimicarb-containing commercial-formulation carbamate insecticide Aficida® (50% pirimicarb) were evaluated on Rhinella arenarum (Anura, Bufonidae) tadpoles exposed under laboratory conditions. Lethal and sublethal effects were employed as bioassays for acute toxicity, whereas micronuclei (MNi) induction and alterations in the ratio erythrocytes:erythroblasts were employed as end-points for genotoxicity and cytotoxicity, respectively. Cr(VI) (23 mg L-1) and cyclophosphamide (40 mg L-1) were employed as positive controls for toxicity and geno-cytotoxicity assays, respectively. In Gosner stage 25 (STD25), the results revealed mean values of 402.0 and 223.6 mg Aficida® L-1 for LC-5024 h and LC-5096 h, respectively. When STD37-39 tadpoles were exposed, the LC-5024 h and LC-5096 h reached values of 239.4 and 181.7 mg Aficida® L-1, respectively. Sublethal effects revealed a mean EC-5096 h of 133.85 and 104.2 mg Aficida® in those STD25 and STD37-39 treated tadpoles, respectively. The results demonstrated that in 48-h-exposed tadpoles, a MNi increase was found only in those 80.0 mg L-1 Aficida®-treated individuals. When tadpoles were exposed to Aficida® for 96 h, only the 160 mg L-1-treated individuals showed a significant increase in MNi frequency. Concentrations ranging from 80.0 to 250.0 mg Aficida® L-1 resulted in cellular cytotoxicity, revealed by a decreased proportion of circulating erythrocytes and an enhancement of erythroblasts. Accordingly, this species could provide a suitable and useful experimental model for biomonitoring aquatic ecosystems. © 2009 Elsevier Ltd. All rights reserved.
Fil: Vera Candioti, Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Naturales y Museo. Laboratorio de Citogenetica; Argentina
Fil: Natale, Guillermo Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina
Fil: Soloneski, Sonia Maria Elsa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Naturales y Museo. Laboratorio de Citogenetica; Argentina
Fil: Ronco, Alicia Estela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina
Fil: Larramendy, Marcelo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Naturales y Museo. Laboratorio de Citogenetica; Argentina
Materia
Carbamates
Cytotoxicity
Erythrocyte:Erythroblast Ratio
Genotoxicity
Micronuclei
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/66364
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/66364
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Sublethal and lethal effects on Rhinella Arenarum (Anura, Bufonidae) tadpoles exerted by the pirimicarb-containing technical formulation insecticide Aficida®Vera Candioti, JosefinaNatale, Guillermo SebastianSoloneski, Sonia Maria ElsaRonco, Alicia EstelaLarramendy, Marcelo LuisCarbamatesCytotoxicityErythrocyte:Erythroblast RatioGenotoxicityMicronucleihttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Acute toxicity, genotoxicity, and cytotoxicity of the pirimicarb-containing commercial-formulation carbamate insecticide Aficida® (50% pirimicarb) were evaluated on Rhinella arenarum (Anura, Bufonidae) tadpoles exposed under laboratory conditions. Lethal and sublethal effects were employed as bioassays for acute toxicity, whereas micronuclei (MNi) induction and alterations in the ratio erythrocytes:erythroblasts were employed as end-points for genotoxicity and cytotoxicity, respectively. Cr(VI) (23 mg L-1) and cyclophosphamide (40 mg L-1) were employed as positive controls for toxicity and geno-cytotoxicity assays, respectively. In Gosner stage 25 (STD25), the results revealed mean values of 402.0 and 223.6 mg Aficida® L-1 for LC-5024 h and LC-5096 h, respectively. When STD37-39 tadpoles were exposed, the LC-5024 h and LC-5096 h reached values of 239.4 and 181.7 mg Aficida® L-1, respectively. Sublethal effects revealed a mean EC-5096 h of 133.85 and 104.2 mg Aficida® in those STD25 and STD37-39 treated tadpoles, respectively. The results demonstrated that in 48-h-exposed tadpoles, a MNi increase was found only in those 80.0 mg L-1 Aficida®-treated individuals. When tadpoles were exposed to Aficida® for 96 h, only the 160 mg L-1-treated individuals showed a significant increase in MNi frequency. Concentrations ranging from 80.0 to 250.0 mg Aficida® L-1 resulted in cellular cytotoxicity, revealed by a decreased proportion of circulating erythrocytes and an enhancement of erythroblasts. Accordingly, this species could provide a suitable and useful experimental model for biomonitoring aquatic ecosystems. © 2009 Elsevier Ltd. All rights reserved.Fil: Vera Candioti, Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Naturales y Museo. Laboratorio de Citogenetica; ArgentinaFil: Natale, Guillermo Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; ArgentinaFil: Soloneski, Sonia Maria Elsa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Naturales y Museo. Laboratorio de Citogenetica; ArgentinaFil: Ronco, Alicia Estela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; ArgentinaFil: Larramendy, Marcelo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Naturales y Museo. Laboratorio de Citogenetica; ArgentinaPergamon-Elsevier Science Ltd2010-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/66364Vera Candioti, Josefina; Natale, Guillermo Sebastian; Soloneski, Sonia Maria Elsa; Ronco, Alicia Estela; Larramendy, Marcelo Luis; Sublethal and lethal effects on Rhinella Arenarum (Anura, Bufonidae) tadpoles exerted by the pirimicarb-containing technical formulation insecticide Aficida®; Pergamon-Elsevier Science Ltd; Chemosphere; 78; 3; 1-2010; 249-2550045-6535CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0045653509012909info:eu-repo/semantics/altIdentifier/doi/10.1016/j.chemosphere.2009.10.064info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:51:06Zoai:ri.conicet.gov.ar:11336/66364instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:51:06.439CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Sublethal and lethal effects on Rhinella Arenarum (Anura, Bufonidae) tadpoles exerted by the pirimicarb-containing technical formulation insecticide Aficida®
title Sublethal and lethal effects on Rhinella Arenarum (Anura, Bufonidae) tadpoles exerted by the pirimicarb-containing technical formulation insecticide Aficida®
spellingShingle Sublethal and lethal effects on Rhinella Arenarum (Anura, Bufonidae) tadpoles exerted by the pirimicarb-containing technical formulation insecticide Aficida®
Vera Candioti, Josefina
Carbamates
Cytotoxicity
Erythrocyte:Erythroblast Ratio
Genotoxicity
Micronuclei
title_short Sublethal and lethal effects on Rhinella Arenarum (Anura, Bufonidae) tadpoles exerted by the pirimicarb-containing technical formulation insecticide Aficida®
title_full Sublethal and lethal effects on Rhinella Arenarum (Anura, Bufonidae) tadpoles exerted by the pirimicarb-containing technical formulation insecticide Aficida®
title_fullStr Sublethal and lethal effects on Rhinella Arenarum (Anura, Bufonidae) tadpoles exerted by the pirimicarb-containing technical formulation insecticide Aficida®
title_full_unstemmed Sublethal and lethal effects on Rhinella Arenarum (Anura, Bufonidae) tadpoles exerted by the pirimicarb-containing technical formulation insecticide Aficida®
title_sort Sublethal and lethal effects on Rhinella Arenarum (Anura, Bufonidae) tadpoles exerted by the pirimicarb-containing technical formulation insecticide Aficida®
dc.creator.none.fl_str_mv Vera Candioti, Josefina
Natale, Guillermo Sebastian
Soloneski, Sonia Maria Elsa
Ronco, Alicia Estela
Larramendy, Marcelo Luis
author Vera Candioti, Josefina
author_facet Vera Candioti, Josefina
Natale, Guillermo Sebastian
Soloneski, Sonia Maria Elsa
Ronco, Alicia Estela
Larramendy, Marcelo Luis
author_role author
author2 Natale, Guillermo Sebastian
Soloneski, Sonia Maria Elsa
Ronco, Alicia Estela
Larramendy, Marcelo Luis
author2_role author
author
author
author
dc.subject.none.fl_str_mv Carbamates
Cytotoxicity
Erythrocyte:Erythroblast Ratio
Genotoxicity
Micronuclei
topic Carbamates
Cytotoxicity
Erythrocyte:Erythroblast Ratio
Genotoxicity
Micronuclei
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Acute toxicity, genotoxicity, and cytotoxicity of the pirimicarb-containing commercial-formulation carbamate insecticide Aficida® (50% pirimicarb) were evaluated on Rhinella arenarum (Anura, Bufonidae) tadpoles exposed under laboratory conditions. Lethal and sublethal effects were employed as bioassays for acute toxicity, whereas micronuclei (MNi) induction and alterations in the ratio erythrocytes:erythroblasts were employed as end-points for genotoxicity and cytotoxicity, respectively. Cr(VI) (23 mg L-1) and cyclophosphamide (40 mg L-1) were employed as positive controls for toxicity and geno-cytotoxicity assays, respectively. In Gosner stage 25 (STD25), the results revealed mean values of 402.0 and 223.6 mg Aficida® L-1 for LC-5024 h and LC-5096 h, respectively. When STD37-39 tadpoles were exposed, the LC-5024 h and LC-5096 h reached values of 239.4 and 181.7 mg Aficida® L-1, respectively. Sublethal effects revealed a mean EC-5096 h of 133.85 and 104.2 mg Aficida® in those STD25 and STD37-39 treated tadpoles, respectively. The results demonstrated that in 48-h-exposed tadpoles, a MNi increase was found only in those 80.0 mg L-1 Aficida®-treated individuals. When tadpoles were exposed to Aficida® for 96 h, only the 160 mg L-1-treated individuals showed a significant increase in MNi frequency. Concentrations ranging from 80.0 to 250.0 mg Aficida® L-1 resulted in cellular cytotoxicity, revealed by a decreased proportion of circulating erythrocytes and an enhancement of erythroblasts. Accordingly, this species could provide a suitable and useful experimental model for biomonitoring aquatic ecosystems. © 2009 Elsevier Ltd. All rights reserved.
Fil: Vera Candioti, Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Naturales y Museo. Laboratorio de Citogenetica; Argentina
Fil: Natale, Guillermo Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina
Fil: Soloneski, Sonia Maria Elsa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Naturales y Museo. Laboratorio de Citogenetica; Argentina
Fil: Ronco, Alicia Estela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina
Fil: Larramendy, Marcelo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Naturales y Museo. Laboratorio de Citogenetica; Argentina
description Acute toxicity, genotoxicity, and cytotoxicity of the pirimicarb-containing commercial-formulation carbamate insecticide Aficida® (50% pirimicarb) were evaluated on Rhinella arenarum (Anura, Bufonidae) tadpoles exposed under laboratory conditions. Lethal and sublethal effects were employed as bioassays for acute toxicity, whereas micronuclei (MNi) induction and alterations in the ratio erythrocytes:erythroblasts were employed as end-points for genotoxicity and cytotoxicity, respectively. Cr(VI) (23 mg L-1) and cyclophosphamide (40 mg L-1) were employed as positive controls for toxicity and geno-cytotoxicity assays, respectively. In Gosner stage 25 (STD25), the results revealed mean values of 402.0 and 223.6 mg Aficida® L-1 for LC-5024 h and LC-5096 h, respectively. When STD37-39 tadpoles were exposed, the LC-5024 h and LC-5096 h reached values of 239.4 and 181.7 mg Aficida® L-1, respectively. Sublethal effects revealed a mean EC-5096 h of 133.85 and 104.2 mg Aficida® in those STD25 and STD37-39 treated tadpoles, respectively. The results demonstrated that in 48-h-exposed tadpoles, a MNi increase was found only in those 80.0 mg L-1 Aficida®-treated individuals. When tadpoles were exposed to Aficida® for 96 h, only the 160 mg L-1-treated individuals showed a significant increase in MNi frequency. Concentrations ranging from 80.0 to 250.0 mg Aficida® L-1 resulted in cellular cytotoxicity, revealed by a decreased proportion of circulating erythrocytes and an enhancement of erythroblasts. Accordingly, this species could provide a suitable and useful experimental model for biomonitoring aquatic ecosystems. © 2009 Elsevier Ltd. All rights reserved.
publishDate 2010
dc.date.none.fl_str_mv 2010-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/66364
Vera Candioti, Josefina; Natale, Guillermo Sebastian; Soloneski, Sonia Maria Elsa; Ronco, Alicia Estela; Larramendy, Marcelo Luis; Sublethal and lethal effects on Rhinella Arenarum (Anura, Bufonidae) tadpoles exerted by the pirimicarb-containing technical formulation insecticide Aficida®; Pergamon-Elsevier Science Ltd; Chemosphere; 78; 3; 1-2010; 249-255
0045-6535
CONICET Digital
CONICET
url http://hdl.handle.net/11336/66364
identifier_str_mv Vera Candioti, Josefina; Natale, Guillermo Sebastian; Soloneski, Sonia Maria Elsa; Ronco, Alicia Estela; Larramendy, Marcelo Luis; Sublethal and lethal effects on Rhinella Arenarum (Anura, Bufonidae) tadpoles exerted by the pirimicarb-containing technical formulation insecticide Aficida®; Pergamon-Elsevier Science Ltd; Chemosphere; 78; 3; 1-2010; 249-255
0045-6535
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0045653509012909
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.chemosphere.2009.10.064
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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