Identification of Diatraea spp. (Lepidoptera: Crambidae) based on cytochrome oxidase II

Autores
Barrera, Gloria Patricia; Villamizar, Laura Fernanda; Espinel, Carlos; Quintero, Edgar Mauricio; Belaich, Mariano Nicolas; Toloza, Deisy Liseth; Ghiringhelli, Pablo Daniel; Vargas, Germán
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Diatraea spp. (Lepidoptera: Crambidae) are a group of insects that are agriculture pests in many economically relevant crops such as sugarcane, sorghum, corn and rice. Recognized species for this genus respond differentially to natural enemies used in their biological control, emphasizing the importance of species in a regional approach. Currently, identification is based on the male genitalia. However, the availability of specimens collected from field and subjectivity based on the character recognition can seriously hamper species identification, and therefore result in inadequate pest management. To overcome this, individuals of Diatraea spp. preliminarily classified male genitalia and obtained from reared conditions and the field (both derived from natural populations occurring in Colombia) were analyzed using genitalic morphometry and molecular biology specifically using a fragment of the cytochrome oxidase subunit II (CO II) mitochondrial gene. Although morphometric analysis did not show any overriding results regarding genitalia morphology, the bioinformatics analyses of CO II sequences resulted in an adequate classification of the individuals within the recognized species. It also, revealed that the occurrence of clades associated with geographical distribution may be associated with cryptic species. The latter was also confirmed by a Single-Strand Conformation Polymorphism (SSCP) methodology evaluating the same fragment of CO II. This experimental approach allows properly recognizing each species and in consequence is proposed as an effective tool in Diatraea species identification.
Fil: Barrera, Gloria Patricia. Corporación Colombiana de Investigación Agropecuaria; Colombia
Fil: Villamizar, Laura Fernanda. Corporación Colombiana de Investigación Agropecuaria; Colombia. AgResearch Ltd. Lincoln Research Centre; Nueva Zelanda
Fil: Espinel, Carlos. Corporación Colombiana de Investigación Agropecuaria; Colombia
Fil: Quintero, Edgar Mauricio. Centro de Investigación de la Caña de Azúcar de Colombia; Colombia
Fil: Belaich, Mariano Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Ingeniería Genética y Biología Molecular y Celular. Área de Virosis Emergentes y Zoonótica; Argentina
Fil: Toloza, Deisy Liseth. Corporación Colombiana de Investigación Agropecuaria; Colombia
Fil: Ghiringhelli, Pablo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Ingeniería Genética y Biología Molecular y Celular. Área de Virosis Emergentes y Zoonótica; Argentina
Fil: Vargas, Germán. Centro de Investigación de la Caña de Azúcar de Colombia; Colombia
Materia
DIATRAEA
COII TAXONOMY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/41202
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/41202
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Identification of Diatraea spp. (Lepidoptera: Crambidae) based on cytochrome oxidase IIBarrera, Gloria PatriciaVillamizar, Laura FernandaEspinel, CarlosQuintero, Edgar MauricioBelaich, Mariano NicolasToloza, Deisy LisethGhiringhelli, Pablo DanielVargas, GermánDIATRAEACOII TAXONOMYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Diatraea spp. (Lepidoptera: Crambidae) are a group of insects that are agriculture pests in many economically relevant crops such as sugarcane, sorghum, corn and rice. Recognized species for this genus respond differentially to natural enemies used in their biological control, emphasizing the importance of species in a regional approach. Currently, identification is based on the male genitalia. However, the availability of specimens collected from field and subjectivity based on the character recognition can seriously hamper species identification, and therefore result in inadequate pest management. To overcome this, individuals of Diatraea spp. preliminarily classified male genitalia and obtained from reared conditions and the field (both derived from natural populations occurring in Colombia) were analyzed using genitalic morphometry and molecular biology specifically using a fragment of the cytochrome oxidase subunit II (CO II) mitochondrial gene. Although morphometric analysis did not show any overriding results regarding genitalia morphology, the bioinformatics analyses of CO II sequences resulted in an adequate classification of the individuals within the recognized species. It also, revealed that the occurrence of clades associated with geographical distribution may be associated with cryptic species. The latter was also confirmed by a Single-Strand Conformation Polymorphism (SSCP) methodology evaluating the same fragment of CO II. This experimental approach allows properly recognizing each species and in consequence is proposed as an effective tool in Diatraea species identification.Fil: Barrera, Gloria Patricia. Corporación Colombiana de Investigación Agropecuaria; ColombiaFil: Villamizar, Laura Fernanda. Corporación Colombiana de Investigación Agropecuaria; Colombia. AgResearch Ltd. Lincoln Research Centre; Nueva ZelandaFil: Espinel, Carlos. Corporación Colombiana de Investigación Agropecuaria; ColombiaFil: Quintero, Edgar Mauricio. Centro de Investigación de la Caña de Azúcar de Colombia; ColombiaFil: Belaich, Mariano Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Ingeniería Genética y Biología Molecular y Celular. Área de Virosis Emergentes y Zoonótica; ArgentinaFil: Toloza, Deisy Liseth. Corporación Colombiana de Investigación Agropecuaria; ColombiaFil: Ghiringhelli, Pablo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Ingeniería Genética y Biología Molecular y Celular. Área de Virosis Emergentes y Zoonótica; ArgentinaFil: Vargas, Germán. Centro de Investigación de la Caña de Azúcar de Colombia; ColombiaPublic Library of Science2017-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/41202Barrera, Gloria Patricia; Villamizar, Laura Fernanda; Espinel, Carlos; Quintero, Edgar Mauricio; Belaich, Mariano Nicolas; et al.; Identification of Diatraea spp. (Lepidoptera: Crambidae) based on cytochrome oxidase II; Public Library of Science; Plos One; 12; 9; 9-20171932-6203CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article/related?id=10.1371/journal.pone.0184053info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0184053info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:21:39Zoai:ri.conicet.gov.ar:11336/41202instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:21:39.294CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Identification of Diatraea spp. (Lepidoptera: Crambidae) based on cytochrome oxidase II
title Identification of Diatraea spp. (Lepidoptera: Crambidae) based on cytochrome oxidase II
spellingShingle Identification of Diatraea spp. (Lepidoptera: Crambidae) based on cytochrome oxidase II
Barrera, Gloria Patricia
DIATRAEA
COII TAXONOMY
title_short Identification of Diatraea spp. (Lepidoptera: Crambidae) based on cytochrome oxidase II
title_full Identification of Diatraea spp. (Lepidoptera: Crambidae) based on cytochrome oxidase II
title_fullStr Identification of Diatraea spp. (Lepidoptera: Crambidae) based on cytochrome oxidase II
title_full_unstemmed Identification of Diatraea spp. (Lepidoptera: Crambidae) based on cytochrome oxidase II
title_sort Identification of Diatraea spp. (Lepidoptera: Crambidae) based on cytochrome oxidase II
dc.creator.none.fl_str_mv Barrera, Gloria Patricia
Villamizar, Laura Fernanda
Espinel, Carlos
Quintero, Edgar Mauricio
Belaich, Mariano Nicolas
Toloza, Deisy Liseth
Ghiringhelli, Pablo Daniel
Vargas, Germán
author Barrera, Gloria Patricia
author_facet Barrera, Gloria Patricia
Villamizar, Laura Fernanda
Espinel, Carlos
Quintero, Edgar Mauricio
Belaich, Mariano Nicolas
Toloza, Deisy Liseth
Ghiringhelli, Pablo Daniel
Vargas, Germán
author_role author
author2 Villamizar, Laura Fernanda
Espinel, Carlos
Quintero, Edgar Mauricio
Belaich, Mariano Nicolas
Toloza, Deisy Liseth
Ghiringhelli, Pablo Daniel
Vargas, Germán
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv DIATRAEA
COII TAXONOMY
topic DIATRAEA
COII TAXONOMY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Diatraea spp. (Lepidoptera: Crambidae) are a group of insects that are agriculture pests in many economically relevant crops such as sugarcane, sorghum, corn and rice. Recognized species for this genus respond differentially to natural enemies used in their biological control, emphasizing the importance of species in a regional approach. Currently, identification is based on the male genitalia. However, the availability of specimens collected from field and subjectivity based on the character recognition can seriously hamper species identification, and therefore result in inadequate pest management. To overcome this, individuals of Diatraea spp. preliminarily classified male genitalia and obtained from reared conditions and the field (both derived from natural populations occurring in Colombia) were analyzed using genitalic morphometry and molecular biology specifically using a fragment of the cytochrome oxidase subunit II (CO II) mitochondrial gene. Although morphometric analysis did not show any overriding results regarding genitalia morphology, the bioinformatics analyses of CO II sequences resulted in an adequate classification of the individuals within the recognized species. It also, revealed that the occurrence of clades associated with geographical distribution may be associated with cryptic species. The latter was also confirmed by a Single-Strand Conformation Polymorphism (SSCP) methodology evaluating the same fragment of CO II. This experimental approach allows properly recognizing each species and in consequence is proposed as an effective tool in Diatraea species identification.
Fil: Barrera, Gloria Patricia. Corporación Colombiana de Investigación Agropecuaria; Colombia
Fil: Villamizar, Laura Fernanda. Corporación Colombiana de Investigación Agropecuaria; Colombia. AgResearch Ltd. Lincoln Research Centre; Nueva Zelanda
Fil: Espinel, Carlos. Corporación Colombiana de Investigación Agropecuaria; Colombia
Fil: Quintero, Edgar Mauricio. Centro de Investigación de la Caña de Azúcar de Colombia; Colombia
Fil: Belaich, Mariano Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Ingeniería Genética y Biología Molecular y Celular. Área de Virosis Emergentes y Zoonótica; Argentina
Fil: Toloza, Deisy Liseth. Corporación Colombiana de Investigación Agropecuaria; Colombia
Fil: Ghiringhelli, Pablo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Ingeniería Genética y Biología Molecular y Celular. Área de Virosis Emergentes y Zoonótica; Argentina
Fil: Vargas, Germán. Centro de Investigación de la Caña de Azúcar de Colombia; Colombia
description Diatraea spp. (Lepidoptera: Crambidae) are a group of insects that are agriculture pests in many economically relevant crops such as sugarcane, sorghum, corn and rice. Recognized species for this genus respond differentially to natural enemies used in their biological control, emphasizing the importance of species in a regional approach. Currently, identification is based on the male genitalia. However, the availability of specimens collected from field and subjectivity based on the character recognition can seriously hamper species identification, and therefore result in inadequate pest management. To overcome this, individuals of Diatraea spp. preliminarily classified male genitalia and obtained from reared conditions and the field (both derived from natural populations occurring in Colombia) were analyzed using genitalic morphometry and molecular biology specifically using a fragment of the cytochrome oxidase subunit II (CO II) mitochondrial gene. Although morphometric analysis did not show any overriding results regarding genitalia morphology, the bioinformatics analyses of CO II sequences resulted in an adequate classification of the individuals within the recognized species. It also, revealed that the occurrence of clades associated with geographical distribution may be associated with cryptic species. The latter was also confirmed by a Single-Strand Conformation Polymorphism (SSCP) methodology evaluating the same fragment of CO II. This experimental approach allows properly recognizing each species and in consequence is proposed as an effective tool in Diatraea species identification.
publishDate 2017
dc.date.none.fl_str_mv 2017-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/41202
Barrera, Gloria Patricia; Villamizar, Laura Fernanda; Espinel, Carlos; Quintero, Edgar Mauricio; Belaich, Mariano Nicolas; et al.; Identification of Diatraea spp. (Lepidoptera: Crambidae) based on cytochrome oxidase II; Public Library of Science; Plos One; 12; 9; 9-2017
1932-6203
CONICET Digital
CONICET
url http://hdl.handle.net/11336/41202
identifier_str_mv Barrera, Gloria Patricia; Villamizar, Laura Fernanda; Espinel, Carlos; Quintero, Edgar Mauricio; Belaich, Mariano Nicolas; et al.; Identification of Diatraea spp. (Lepidoptera: Crambidae) based on cytochrome oxidase II; Public Library of Science; Plos One; 12; 9; 9-2017
1932-6203
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article/related?id=10.1371/journal.pone.0184053
info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0184053
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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