Co-assembly of Azospirillum–Pseudomonas biofilms in the rhizosphere enhances lettuce root colonization, growth, and heat-stress resilience

Autores
Diaz, Pablo Rafael; de Gerónimo, Eduardo; Borrajo, María Paula; Labarthe, María Mercedes; Martino, María Verónica; Creus, Cecilia Mónica; Maroniche, Guillermo Andrés
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Understanding plant growth-promoting bacteria interaction is essential for developing of effective multi-strain inoculants. Here, we investigated how Azospirillum baldaniorum Sp245 and Pseudomonas fluorescens A506 interact when establishing biofilms under rhizospheric conditions and its impact on root colonization and plant growth. Mixed biofilms assembled in vitro on root exudates revealed competition between both strains, with Sp245 outcompeting A506. On lettuce roots, they formed spatially segregated biofilms according to their individual niche preferences: Sp245 exhibited dense biofilms on and along the main root, while A506 grew preferentially associated to root hairs. Both strains co-localized only in certain hotspots on the root surface and hairs bases. Yet when colonizing roots in substrate, their colonization was mutually enhanced, suggesting that cooperation prevails under these conditions. Co-inoculation of Sp245 and A506 promoted lettuce growth synergistically, increasing leaf area, fresh and dry biomass, and root dry weight. Moreover, co-inoculated plants showed enhanced survival and growth after heat stress. Our findings unveil a complex yet complementary interaction between Sp245 and A506 in the rhizosphere, where their spatial segregation does not preclude cooperation and synergistic plant-beneficial effects. Likewise, the results highlight the potential of simplified two-strain synthetic communities for enhancing crop productivity and resilience under climatic stress.
Fil: Diaz, Pablo Rafael. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina
Fil: de Gerónimo, Eduardo. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Innovación Para la Producción Agropecuaria y El Desarrollo Sostenible. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Buenos Aires Sur. Estacion Experimental Agropecuaria Balcarce. Instituto de Innovación Para la Producción Agropecuaria y El Desarrollo Sostenible.; Argentina
Fil: Borrajo, María Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Labarthe, María Mercedes. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina
Fil: Martino, María Verónica. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Creus, Cecilia Mónica. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Maroniche, Guillermo Andrés. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina
Materia
plant growth-promoting bacteria
biofilm
consortium
heat stress
exudates
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/281166
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/281166
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Co-assembly of Azospirillum–Pseudomonas biofilms in the rhizosphere enhances lettuce root colonization, growth, and heat-stress resilienceDiaz, Pablo Rafaelde Gerónimo, EduardoBorrajo, María PaulaLabarthe, María MercedesMartino, María VerónicaCreus, Cecilia MónicaMaroniche, Guillermo Andrésplant growth-promoting bacteriabiofilmconsortiumheat stressexudateshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Understanding plant growth-promoting bacteria interaction is essential for developing of effective multi-strain inoculants. Here, we investigated how Azospirillum baldaniorum Sp245 and Pseudomonas fluorescens A506 interact when establishing biofilms under rhizospheric conditions and its impact on root colonization and plant growth. Mixed biofilms assembled in vitro on root exudates revealed competition between both strains, with Sp245 outcompeting A506. On lettuce roots, they formed spatially segregated biofilms according to their individual niche preferences: Sp245 exhibited dense biofilms on and along the main root, while A506 grew preferentially associated to root hairs. Both strains co-localized only in certain hotspots on the root surface and hairs bases. Yet when colonizing roots in substrate, their colonization was mutually enhanced, suggesting that cooperation prevails under these conditions. Co-inoculation of Sp245 and A506 promoted lettuce growth synergistically, increasing leaf area, fresh and dry biomass, and root dry weight. Moreover, co-inoculated plants showed enhanced survival and growth after heat stress. Our findings unveil a complex yet complementary interaction between Sp245 and A506 in the rhizosphere, where their spatial segregation does not preclude cooperation and synergistic plant-beneficial effects. Likewise, the results highlight the potential of simplified two-strain synthetic communities for enhancing crop productivity and resilience under climatic stress.Fil: Diaz, Pablo Rafael. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; ArgentinaFil: de Gerónimo, Eduardo. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Innovación Para la Producción Agropecuaria y El Desarrollo Sostenible. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Buenos Aires Sur. Estacion Experimental Agropecuaria Balcarce. Instituto de Innovación Para la Producción Agropecuaria y El Desarrollo Sostenible.; ArgentinaFil: Borrajo, María Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; ArgentinaFil: Labarthe, María Mercedes. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; ArgentinaFil: Martino, María Verónica. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; ArgentinaFil: Creus, Cecilia Mónica. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; ArgentinaFil: Maroniche, Guillermo Andrés. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; ArgentinaWiley Blackwell Publishing, Inc2025-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/281166Diaz, Pablo Rafael; de Gerónimo, Eduardo; Borrajo, María Paula; Labarthe, María Mercedes; Martino, María Verónica; et al.; Co-assembly of Azospirillum–Pseudomonas biofilms in the rhizosphere enhances lettuce root colonization, growth, and heat-stress resilience; Wiley Blackwell Publishing, Inc; Fems Microbiology Ecology; 101; 12; 11-2025; 1-190168-64961574-6941CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/femsec/advance-article/doi/10.1093/femsec/fiaf113/8327624info:eu-repo/semantics/altIdentifier/doi/10.1093/femsec/fiaf113info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-04-15T10:44:46Zoai:ri.conicet.gov.ar:11336/281166instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-04-15 10:44:47.259CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Co-assembly of Azospirillum–Pseudomonas biofilms in the rhizosphere enhances lettuce root colonization, growth, and heat-stress resilience
title Co-assembly of Azospirillum–Pseudomonas biofilms in the rhizosphere enhances lettuce root colonization, growth, and heat-stress resilience
spellingShingle Co-assembly of Azospirillum–Pseudomonas biofilms in the rhizosphere enhances lettuce root colonization, growth, and heat-stress resilience
Diaz, Pablo Rafael
plant growth-promoting bacteria
biofilm
consortium
heat stress
exudates
title_short Co-assembly of Azospirillum–Pseudomonas biofilms in the rhizosphere enhances lettuce root colonization, growth, and heat-stress resilience
title_full Co-assembly of Azospirillum–Pseudomonas biofilms in the rhizosphere enhances lettuce root colonization, growth, and heat-stress resilience
title_fullStr Co-assembly of Azospirillum–Pseudomonas biofilms in the rhizosphere enhances lettuce root colonization, growth, and heat-stress resilience
title_full_unstemmed Co-assembly of Azospirillum–Pseudomonas biofilms in the rhizosphere enhances lettuce root colonization, growth, and heat-stress resilience
title_sort Co-assembly of Azospirillum–Pseudomonas biofilms in the rhizosphere enhances lettuce root colonization, growth, and heat-stress resilience
dc.creator.none.fl_str_mv Diaz, Pablo Rafael
de Gerónimo, Eduardo
Borrajo, María Paula
Labarthe, María Mercedes
Martino, María Verónica
Creus, Cecilia Mónica
Maroniche, Guillermo Andrés
author Diaz, Pablo Rafael
author_facet Diaz, Pablo Rafael
de Gerónimo, Eduardo
Borrajo, María Paula
Labarthe, María Mercedes
Martino, María Verónica
Creus, Cecilia Mónica
Maroniche, Guillermo Andrés
author_role author
author2 de Gerónimo, Eduardo
Borrajo, María Paula
Labarthe, María Mercedes
Martino, María Verónica
Creus, Cecilia Mónica
Maroniche, Guillermo Andrés
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv plant growth-promoting bacteria
biofilm
consortium
heat stress
exudates
topic plant growth-promoting bacteria
biofilm
consortium
heat stress
exudates
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Understanding plant growth-promoting bacteria interaction is essential for developing of effective multi-strain inoculants. Here, we investigated how Azospirillum baldaniorum Sp245 and Pseudomonas fluorescens A506 interact when establishing biofilms under rhizospheric conditions and its impact on root colonization and plant growth. Mixed biofilms assembled in vitro on root exudates revealed competition between both strains, with Sp245 outcompeting A506. On lettuce roots, they formed spatially segregated biofilms according to their individual niche preferences: Sp245 exhibited dense biofilms on and along the main root, while A506 grew preferentially associated to root hairs. Both strains co-localized only in certain hotspots on the root surface and hairs bases. Yet when colonizing roots in substrate, their colonization was mutually enhanced, suggesting that cooperation prevails under these conditions. Co-inoculation of Sp245 and A506 promoted lettuce growth synergistically, increasing leaf area, fresh and dry biomass, and root dry weight. Moreover, co-inoculated plants showed enhanced survival and growth after heat stress. Our findings unveil a complex yet complementary interaction between Sp245 and A506 in the rhizosphere, where their spatial segregation does not preclude cooperation and synergistic plant-beneficial effects. Likewise, the results highlight the potential of simplified two-strain synthetic communities for enhancing crop productivity and resilience under climatic stress.
Fil: Diaz, Pablo Rafael. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina
Fil: de Gerónimo, Eduardo. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Innovación Para la Producción Agropecuaria y El Desarrollo Sostenible. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Buenos Aires Sur. Estacion Experimental Agropecuaria Balcarce. Instituto de Innovación Para la Producción Agropecuaria y El Desarrollo Sostenible.; Argentina
Fil: Borrajo, María Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Labarthe, María Mercedes. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina
Fil: Martino, María Verónica. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Creus, Cecilia Mónica. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Maroniche, Guillermo Andrés. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina
description Understanding plant growth-promoting bacteria interaction is essential for developing of effective multi-strain inoculants. Here, we investigated how Azospirillum baldaniorum Sp245 and Pseudomonas fluorescens A506 interact when establishing biofilms under rhizospheric conditions and its impact on root colonization and plant growth. Mixed biofilms assembled in vitro on root exudates revealed competition between both strains, with Sp245 outcompeting A506. On lettuce roots, they formed spatially segregated biofilms according to their individual niche preferences: Sp245 exhibited dense biofilms on and along the main root, while A506 grew preferentially associated to root hairs. Both strains co-localized only in certain hotspots on the root surface and hairs bases. Yet when colonizing roots in substrate, their colonization was mutually enhanced, suggesting that cooperation prevails under these conditions. Co-inoculation of Sp245 and A506 promoted lettuce growth synergistically, increasing leaf area, fresh and dry biomass, and root dry weight. Moreover, co-inoculated plants showed enhanced survival and growth after heat stress. Our findings unveil a complex yet complementary interaction between Sp245 and A506 in the rhizosphere, where their spatial segregation does not preclude cooperation and synergistic plant-beneficial effects. Likewise, the results highlight the potential of simplified two-strain synthetic communities for enhancing crop productivity and resilience under climatic stress.
publishDate 2025
dc.date.none.fl_str_mv 2025-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/281166
Diaz, Pablo Rafael; de Gerónimo, Eduardo; Borrajo, María Paula; Labarthe, María Mercedes; Martino, María Verónica; et al.; Co-assembly of Azospirillum–Pseudomonas biofilms in the rhizosphere enhances lettuce root colonization, growth, and heat-stress resilience; Wiley Blackwell Publishing, Inc; Fems Microbiology Ecology; 101; 12; 11-2025; 1-19
0168-6496
1574-6941
CONICET Digital
CONICET
url http://hdl.handle.net/11336/281166
identifier_str_mv Diaz, Pablo Rafael; de Gerónimo, Eduardo; Borrajo, María Paula; Labarthe, María Mercedes; Martino, María Verónica; et al.; Co-assembly of Azospirillum–Pseudomonas biofilms in the rhizosphere enhances lettuce root colonization, growth, and heat-stress resilience; Wiley Blackwell Publishing, Inc; Fems Microbiology Ecology; 101; 12; 11-2025; 1-19
0168-6496
1574-6941
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/femsec/advance-article/doi/10.1093/femsec/fiaf113/8327624
info:eu-repo/semantics/altIdentifier/doi/10.1093/femsec/fiaf113
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.203462

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