Structural and Kinetic Visualization of the Protein Corona on Bioceramic Nanoparticle

Autores
Rial, Ramón; Tichnell, Brandon; Latimer, Brendan; Liu, Zhen; Messina, Paula Verónica; Ruso, Juan M.
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bioceramic nanoparticles exhibit excellent features that enable them to function as an ideal material for hard tissue engineering. However, to fully understand their behavior, it is of crucial importance to understand their behavior within the fluids of the human body. To achieve this goal, we have studied the interaction between hydroxyapatite nanorods (HA) and bovine serum albumin (BSA). First, we describe the surface morphology of the nanoparticle. Then, the main characteristics of the physiological interplay of BSA and the hydroxyapatite nanoparticle are presented by using a battery of techniques: ITC, zeta potential, UV–vis, fluorescence, and CD. Experimental data was analyzed by developing specific approaches to determining important parameters such as rates, affinities, and stochiometries of protein associated with the nanoparticles. ITC has been confirmed as a powerful technique for determining the affinity, binding, and thermodynamics of BSA–nanoparticle interactions. Careful quantitative assessment of the kinetic properties of the adsorption were revealed by UV–vis and fluorescence measurements. Finally, CD measurements highlight the important role of protein flexibility in these kinds of systems.
Fil: Rial, Ramón. Universidad de Santiago de Compostela; España
Fil: Tichnell, Brandon. Frostburg State University; Estados Unidos
Fil: Latimer, Brendan. Frostburg State University; Estados Unidos
Fil: Liu, Zhen. Frostburg State University; Estados Unidos
Fil: Messina, Paula Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Ruso, Juan M.. Universidad de Santiago de Compostela; España
Materia
BSA
HYDROXYAPATITE
NANOPARTICLES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/91347
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Structural and Kinetic Visualization of the Protein Corona on Bioceramic NanoparticleRial, RamónTichnell, BrandonLatimer, BrendanLiu, ZhenMessina, Paula VerónicaRuso, Juan M.BSAHYDROXYAPATITENANOPARTICLEShttps://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2Bioceramic nanoparticles exhibit excellent features that enable them to function as an ideal material for hard tissue engineering. However, to fully understand their behavior, it is of crucial importance to understand their behavior within the fluids of the human body. To achieve this goal, we have studied the interaction between hydroxyapatite nanorods (HA) and bovine serum albumin (BSA). First, we describe the surface morphology of the nanoparticle. Then, the main characteristics of the physiological interplay of BSA and the hydroxyapatite nanoparticle are presented by using a battery of techniques: ITC, zeta potential, UV–vis, fluorescence, and CD. Experimental data was analyzed by developing specific approaches to determining important parameters such as rates, affinities, and stochiometries of protein associated with the nanoparticles. ITC has been confirmed as a powerful technique for determining the affinity, binding, and thermodynamics of BSA–nanoparticle interactions. Careful quantitative assessment of the kinetic properties of the adsorption were revealed by UV–vis and fluorescence measurements. Finally, CD measurements highlight the important role of protein flexibility in these kinds of systems.Fil: Rial, Ramón. Universidad de Santiago de Compostela; EspañaFil: Tichnell, Brandon. Frostburg State University; Estados UnidosFil: Latimer, Brendan. Frostburg State University; Estados UnidosFil: Liu, Zhen. Frostburg State University; Estados UnidosFil: Messina, Paula Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; ArgentinaFil: Ruso, Juan M.. Universidad de Santiago de Compostela; EspañaAmerican Chemical Society2018-01-23info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/91347Rial, Ramón; Tichnell, Brandon; Latimer, Brendan; Liu, Zhen; Messina, Paula Verónica; et al.; Structural and Kinetic Visualization of the Protein Corona on Bioceramic Nanoparticle; American Chemical Society; Langmuir; 34; 7; 23-1-2018; 2471-24800743-7463CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/abs/10.1021/acs.langmuir.7b03573info:eu-repo/semantics/altIdentifier/doi/10.1021/acs.langmuir.7b03573info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:11:34Zoai:ri.conicet.gov.ar:11336/91347instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:11:34.618CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Structural and Kinetic Visualization of the Protein Corona on Bioceramic Nanoparticle
title Structural and Kinetic Visualization of the Protein Corona on Bioceramic Nanoparticle
spellingShingle Structural and Kinetic Visualization of the Protein Corona on Bioceramic Nanoparticle
Rial, Ramón
BSA
HYDROXYAPATITE
NANOPARTICLES
title_short Structural and Kinetic Visualization of the Protein Corona on Bioceramic Nanoparticle
title_full Structural and Kinetic Visualization of the Protein Corona on Bioceramic Nanoparticle
title_fullStr Structural and Kinetic Visualization of the Protein Corona on Bioceramic Nanoparticle
title_full_unstemmed Structural and Kinetic Visualization of the Protein Corona on Bioceramic Nanoparticle
title_sort Structural and Kinetic Visualization of the Protein Corona on Bioceramic Nanoparticle
dc.creator.none.fl_str_mv Rial, Ramón
Tichnell, Brandon
Latimer, Brendan
Liu, Zhen
Messina, Paula Verónica
Ruso, Juan M.
author Rial, Ramón
author_facet Rial, Ramón
Tichnell, Brandon
Latimer, Brendan
Liu, Zhen
Messina, Paula Verónica
Ruso, Juan M.
author_role author
author2 Tichnell, Brandon
Latimer, Brendan
Liu, Zhen
Messina, Paula Verónica
Ruso, Juan M.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv BSA
HYDROXYAPATITE
NANOPARTICLES
topic BSA
HYDROXYAPATITE
NANOPARTICLES
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.10
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Bioceramic nanoparticles exhibit excellent features that enable them to function as an ideal material for hard tissue engineering. However, to fully understand their behavior, it is of crucial importance to understand their behavior within the fluids of the human body. To achieve this goal, we have studied the interaction between hydroxyapatite nanorods (HA) and bovine serum albumin (BSA). First, we describe the surface morphology of the nanoparticle. Then, the main characteristics of the physiological interplay of BSA and the hydroxyapatite nanoparticle are presented by using a battery of techniques: ITC, zeta potential, UV–vis, fluorescence, and CD. Experimental data was analyzed by developing specific approaches to determining important parameters such as rates, affinities, and stochiometries of protein associated with the nanoparticles. ITC has been confirmed as a powerful technique for determining the affinity, binding, and thermodynamics of BSA–nanoparticle interactions. Careful quantitative assessment of the kinetic properties of the adsorption were revealed by UV–vis and fluorescence measurements. Finally, CD measurements highlight the important role of protein flexibility in these kinds of systems.
Fil: Rial, Ramón. Universidad de Santiago de Compostela; España
Fil: Tichnell, Brandon. Frostburg State University; Estados Unidos
Fil: Latimer, Brendan. Frostburg State University; Estados Unidos
Fil: Liu, Zhen. Frostburg State University; Estados Unidos
Fil: Messina, Paula Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Ruso, Juan M.. Universidad de Santiago de Compostela; España
description Bioceramic nanoparticles exhibit excellent features that enable them to function as an ideal material for hard tissue engineering. However, to fully understand their behavior, it is of crucial importance to understand their behavior within the fluids of the human body. To achieve this goal, we have studied the interaction between hydroxyapatite nanorods (HA) and bovine serum albumin (BSA). First, we describe the surface morphology of the nanoparticle. Then, the main characteristics of the physiological interplay of BSA and the hydroxyapatite nanoparticle are presented by using a battery of techniques: ITC, zeta potential, UV–vis, fluorescence, and CD. Experimental data was analyzed by developing specific approaches to determining important parameters such as rates, affinities, and stochiometries of protein associated with the nanoparticles. ITC has been confirmed as a powerful technique for determining the affinity, binding, and thermodynamics of BSA–nanoparticle interactions. Careful quantitative assessment of the kinetic properties of the adsorption were revealed by UV–vis and fluorescence measurements. Finally, CD measurements highlight the important role of protein flexibility in these kinds of systems.
publishDate 2018
dc.date.none.fl_str_mv 2018-01-23
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/91347
Rial, Ramón; Tichnell, Brandon; Latimer, Brendan; Liu, Zhen; Messina, Paula Verónica; et al.; Structural and Kinetic Visualization of the Protein Corona on Bioceramic Nanoparticle; American Chemical Society; Langmuir; 34; 7; 23-1-2018; 2471-2480
0743-7463
CONICET Digital
CONICET
url http://hdl.handle.net/11336/91347
identifier_str_mv Rial, Ramón; Tichnell, Brandon; Latimer, Brendan; Liu, Zhen; Messina, Paula Verónica; et al.; Structural and Kinetic Visualization of the Protein Corona on Bioceramic Nanoparticle; American Chemical Society; Langmuir; 34; 7; 23-1-2018; 2471-2480
0743-7463
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/abs/10.1021/acs.langmuir.7b03573
info:eu-repo/semantics/altIdentifier/doi/10.1021/acs.langmuir.7b03573
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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