Phosphonopropionic acid coating as platform for the efficient grafting of (bio)molecules to hydroxyapatite nanoparticles

Autores
Dittler, Maria Laura; Rodriguez, Hernan Bernardo; Cippollone, Mariano; Grillo, Claudia Alejandra; Gonzalez, Monica Cristina
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A new synthesis strategy intended to increase apatite available surface groups for the covalent functionalization with (bio)molecules was developed. To that purpose, hydroxyapatite nanoparticles (Ap) were synthesized and terminated with 3-phosphonopropionic acid (PPA) yielding Ap with surface bound propionic acid through P-C bonds (ApCOO– ). A thorough characterization of ApCOO– by IR spectroscopy, transmission electron microscopy, X-ray diffraction, X-ray photoelectron spectroscopy, thermal gravimetric analysis, and specific surface area determination (BET model), strongly support an efficient surface grafting of the organophosphonates and a hydroxyapatite core of similar morphology and phase composition to that of synthetic Ap. ApCOO– was able to adsorb bovine serum albumin (BSA) up to 0.20 mg m–2 and showed no cytotoxic effects towards Balb/C 3T3 cells. ApCOO– abundant carboxyl surface groups facilitated the particles grafting through stable amide bonds with basic fuchsin (Fu) and tyrosine (Ty) yielding well covered surfaces. Fu-bound hydroxyapatite powders showed ten times stronger fluorescence than free Fu in ethanol, thus expanding the potential uses of Ap as fluorescent sensors in the red region of the visible spectrum. On the other hand, Ty-bound hydroxyapatite powders showed negative surface charges and high stability of the coating in aqueous suspensions. In contrast, Ty-physically adsorbed hydroxyapatite powders rendered positively charged surfaces and an unstable coating.
Fil: Dittler, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Rodriguez, Hernan Bernardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Cippollone, Mariano. YPF - Tecnología; Argentina
Fil: Grillo, Claudia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Gonzalez, Monica Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Materia
Hydroxyapatite Nanoparticles
Propionic Acid-Terminated
Tyrosine-Grafted
Fuchsin-Grafted
Fluorescence
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/141703
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/141703
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Phosphonopropionic acid coating as platform for the efficient grafting of (bio)molecules to hydroxyapatite nanoparticlesDittler, Maria LauraRodriguez, Hernan BernardoCippollone, MarianoGrillo, Claudia AlejandraGonzalez, Monica CristinaHydroxyapatite NanoparticlesPropionic Acid-TerminatedTyrosine-GraftedFuchsin-GraftedFluorescencehttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1A new synthesis strategy intended to increase apatite available surface groups for the covalent functionalization with (bio)molecules was developed. To that purpose, hydroxyapatite nanoparticles (Ap) were synthesized and terminated with 3-phosphonopropionic acid (PPA) yielding Ap with surface bound propionic acid through P-C bonds (ApCOO– ). A thorough characterization of ApCOO– by IR spectroscopy, transmission electron microscopy, X-ray diffraction, X-ray photoelectron spectroscopy, thermal gravimetric analysis, and specific surface area determination (BET model), strongly support an efficient surface grafting of the organophosphonates and a hydroxyapatite core of similar morphology and phase composition to that of synthetic Ap. ApCOO– was able to adsorb bovine serum albumin (BSA) up to 0.20 mg m–2 and showed no cytotoxic effects towards Balb/C 3T3 cells. ApCOO– abundant carboxyl surface groups facilitated the particles grafting through stable amide bonds with basic fuchsin (Fu) and tyrosine (Ty) yielding well covered surfaces. Fu-bound hydroxyapatite powders showed ten times stronger fluorescence than free Fu in ethanol, thus expanding the potential uses of Ap as fluorescent sensors in the red region of the visible spectrum. On the other hand, Ty-bound hydroxyapatite powders showed negative surface charges and high stability of the coating in aqueous suspensions. In contrast, Ty-physically adsorbed hydroxyapatite powders rendered positively charged surfaces and an unstable coating.Fil: Dittler, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaFil: Rodriguez, Hernan Bernardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaFil: Cippollone, Mariano. YPF - Tecnología; ArgentinaFil: Grillo, Claudia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaFil: Gonzalez, Monica Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaAsociación AMG Transcend2020-08-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/141703Dittler, Maria Laura; Rodriguez, Hernan Bernardo; Cippollone, Mariano; Grillo, Claudia Alejandra; Gonzalez, Monica Cristina; Phosphonopropionic acid coating as platform for the efficient grafting of (bio)molecules to hydroxyapatite nanoparticles; Asociación AMG Transcend; Biomedical Engineering International; 2; 1; 4-8-2020; 30-482668-6007CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi//10.33263/BioMed21.030048info:eu-repo/semantics/altIdentifier/url/https://biomedicalengineering.international/phosphonopropionic-acid-coating-as-platform-for-the-efficient-grafting-of-biomolecules-to-hydroxyapatite-nanoparticles/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:21:57Zoai:ri.conicet.gov.ar:11336/141703instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:21:58.073CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Phosphonopropionic acid coating as platform for the efficient grafting of (bio)molecules to hydroxyapatite nanoparticles
title Phosphonopropionic acid coating as platform for the efficient grafting of (bio)molecules to hydroxyapatite nanoparticles
spellingShingle Phosphonopropionic acid coating as platform for the efficient grafting of (bio)molecules to hydroxyapatite nanoparticles
Dittler, Maria Laura
Hydroxyapatite Nanoparticles
Propionic Acid-Terminated
Tyrosine-Grafted
Fuchsin-Grafted
Fluorescence
title_short Phosphonopropionic acid coating as platform for the efficient grafting of (bio)molecules to hydroxyapatite nanoparticles
title_full Phosphonopropionic acid coating as platform for the efficient grafting of (bio)molecules to hydroxyapatite nanoparticles
title_fullStr Phosphonopropionic acid coating as platform for the efficient grafting of (bio)molecules to hydroxyapatite nanoparticles
title_full_unstemmed Phosphonopropionic acid coating as platform for the efficient grafting of (bio)molecules to hydroxyapatite nanoparticles
title_sort Phosphonopropionic acid coating as platform for the efficient grafting of (bio)molecules to hydroxyapatite nanoparticles
dc.creator.none.fl_str_mv Dittler, Maria Laura
Rodriguez, Hernan Bernardo
Cippollone, Mariano
Grillo, Claudia Alejandra
Gonzalez, Monica Cristina
author Dittler, Maria Laura
author_facet Dittler, Maria Laura
Rodriguez, Hernan Bernardo
Cippollone, Mariano
Grillo, Claudia Alejandra
Gonzalez, Monica Cristina
author_role author
author2 Rodriguez, Hernan Bernardo
Cippollone, Mariano
Grillo, Claudia Alejandra
Gonzalez, Monica Cristina
author2_role author
author
author
author
dc.subject.none.fl_str_mv Hydroxyapatite Nanoparticles
Propionic Acid-Terminated
Tyrosine-Grafted
Fuchsin-Grafted
Fluorescence
topic Hydroxyapatite Nanoparticles
Propionic Acid-Terminated
Tyrosine-Grafted
Fuchsin-Grafted
Fluorescence
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A new synthesis strategy intended to increase apatite available surface groups for the covalent functionalization with (bio)molecules was developed. To that purpose, hydroxyapatite nanoparticles (Ap) were synthesized and terminated with 3-phosphonopropionic acid (PPA) yielding Ap with surface bound propionic acid through P-C bonds (ApCOO– ). A thorough characterization of ApCOO– by IR spectroscopy, transmission electron microscopy, X-ray diffraction, X-ray photoelectron spectroscopy, thermal gravimetric analysis, and specific surface area determination (BET model), strongly support an efficient surface grafting of the organophosphonates and a hydroxyapatite core of similar morphology and phase composition to that of synthetic Ap. ApCOO– was able to adsorb bovine serum albumin (BSA) up to 0.20 mg m–2 and showed no cytotoxic effects towards Balb/C 3T3 cells. ApCOO– abundant carboxyl surface groups facilitated the particles grafting through stable amide bonds with basic fuchsin (Fu) and tyrosine (Ty) yielding well covered surfaces. Fu-bound hydroxyapatite powders showed ten times stronger fluorescence than free Fu in ethanol, thus expanding the potential uses of Ap as fluorescent sensors in the red region of the visible spectrum. On the other hand, Ty-bound hydroxyapatite powders showed negative surface charges and high stability of the coating in aqueous suspensions. In contrast, Ty-physically adsorbed hydroxyapatite powders rendered positively charged surfaces and an unstable coating.
Fil: Dittler, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Rodriguez, Hernan Bernardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Cippollone, Mariano. YPF - Tecnología; Argentina
Fil: Grillo, Claudia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Gonzalez, Monica Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
description A new synthesis strategy intended to increase apatite available surface groups for the covalent functionalization with (bio)molecules was developed. To that purpose, hydroxyapatite nanoparticles (Ap) were synthesized and terminated with 3-phosphonopropionic acid (PPA) yielding Ap with surface bound propionic acid through P-C bonds (ApCOO– ). A thorough characterization of ApCOO– by IR spectroscopy, transmission electron microscopy, X-ray diffraction, X-ray photoelectron spectroscopy, thermal gravimetric analysis, and specific surface area determination (BET model), strongly support an efficient surface grafting of the organophosphonates and a hydroxyapatite core of similar morphology and phase composition to that of synthetic Ap. ApCOO– was able to adsorb bovine serum albumin (BSA) up to 0.20 mg m–2 and showed no cytotoxic effects towards Balb/C 3T3 cells. ApCOO– abundant carboxyl surface groups facilitated the particles grafting through stable amide bonds with basic fuchsin (Fu) and tyrosine (Ty) yielding well covered surfaces. Fu-bound hydroxyapatite powders showed ten times stronger fluorescence than free Fu in ethanol, thus expanding the potential uses of Ap as fluorescent sensors in the red region of the visible spectrum. On the other hand, Ty-bound hydroxyapatite powders showed negative surface charges and high stability of the coating in aqueous suspensions. In contrast, Ty-physically adsorbed hydroxyapatite powders rendered positively charged surfaces and an unstable coating.
publishDate 2020
dc.date.none.fl_str_mv 2020-08-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/141703
Dittler, Maria Laura; Rodriguez, Hernan Bernardo; Cippollone, Mariano; Grillo, Claudia Alejandra; Gonzalez, Monica Cristina; Phosphonopropionic acid coating as platform for the efficient grafting of (bio)molecules to hydroxyapatite nanoparticles; Asociación AMG Transcend; Biomedical Engineering International; 2; 1; 4-8-2020; 30-48
2668-6007
CONICET Digital
CONICET
url http://hdl.handle.net/11336/141703
identifier_str_mv Dittler, Maria Laura; Rodriguez, Hernan Bernardo; Cippollone, Mariano; Grillo, Claudia Alejandra; Gonzalez, Monica Cristina; Phosphonopropionic acid coating as platform for the efficient grafting of (bio)molecules to hydroxyapatite nanoparticles; Asociación AMG Transcend; Biomedical Engineering International; 2; 1; 4-8-2020; 30-48
2668-6007
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi//10.33263/BioMed21.030048
info:eu-repo/semantics/altIdentifier/url/https://biomedicalengineering.international/phosphonopropionic-acid-coating-as-platform-for-the-efficient-grafting-of-biomolecules-to-hydroxyapatite-nanoparticles/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Asociación AMG Transcend
publisher.none.fl_str_mv Asociación AMG Transcend
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842981208314609664
score 12.48226

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