Potential of LC coupled to fluorescence detection in food metabolomics: Determination of phenolic compounds in virgin olive oil
- Autores
- Monasterio, Romina Paula; Olmo García, Lucía; Bajoub, Aadil; Fernández Gutiérrez, Alberto; Carrasco Pancorbo, Alegría
- Año de publicación
- 2016
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- A powerful chromatographic method coupled to a fluorescence detector was developed to determine the phenolic compounds present in virgin olive oil (VOO), with the aim to propose an appropriate alternative to liquid chromatography-mass spectrometry. An excitation wavelength of 285 nm was selected and four different emission wavelengths (316, 328, 350 and 450 nm) were simultaneously recorded, working therefore on "multi-emission" detection mode. With the use of commercially available standards and other standards obtained by semipreparative high performance liquid chromatography, it was possible to identify simple phenols, lignans, several complex phenols, and other phenolic compounds present in the matrix under study. A total of 26 phenolic compounds belonging to different chemical families were identified (23 of them were susceptible of being quantified). The proposed methodology provided detection and quantification limits within the ranges of 0.004-7.143 g/mL and 0.013-23.810 g/mL, respectively. As far as the repeatability is concerned, the relative standard deviation values were below 0.43% for retention time, and 9.05% for peak area. The developed methodology was applied for the determination of phenolic compounds in ten VOOs, both monovarietals and blends. Secoiridoids were the most abundant fraction in all the samples, followed by simple phenolic alcohols, lignans, flavonoids, and phenolic acids (being the abundance order of the latter chemical classes logically depending on the variety and origin of the VOOs).
Fil: Monasterio, Romina Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Olmo García, Lucía. Universidad de Granada. Facultad de Ciencias. Departamento de química analítica; España
Fil: Bajoub, Aadil. Universidad de Granada. Facultad de Ciencias. Departamento de química analítica; España
Fil: Fernández Gutiérrez, Alberto. Universidad de Granada. Facultad de Ciencias. Departamento de química analítica; España
Fil: Carrasco Pancorbo, Alegría. Universidad de Granada. Facultad de Ciencias. Departamento de química analítica; España - Materia
-
FLUORESCENCE DETECTION
OLIVE OIL
PHENOLIC COMPOUNDS
SECOIRIDOIDS
FOOD METABOLOMICS - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/23769
Ver los metadatos del registro completo
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oai:ri.conicet.gov.ar:11336/23769 |
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3498 |
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CONICET Digital (CONICET) |
spelling |
Potential of LC coupled to fluorescence detection in food metabolomics: Determination of phenolic compounds in virgin olive oilMonasterio, Romina PaulaOlmo García, LucíaBajoub, AadilFernández Gutiérrez, AlbertoCarrasco Pancorbo, AlegríaFLUORESCENCE DETECTIONOLIVE OILPHENOLIC COMPOUNDSSECOIRIDOIDSFOOD METABOLOMICShttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1A powerful chromatographic method coupled to a fluorescence detector was developed to determine the phenolic compounds present in virgin olive oil (VOO), with the aim to propose an appropriate alternative to liquid chromatography-mass spectrometry. An excitation wavelength of 285 nm was selected and four different emission wavelengths (316, 328, 350 and 450 nm) were simultaneously recorded, working therefore on "multi-emission" detection mode. With the use of commercially available standards and other standards obtained by semipreparative high performance liquid chromatography, it was possible to identify simple phenols, lignans, several complex phenols, and other phenolic compounds present in the matrix under study. A total of 26 phenolic compounds belonging to different chemical families were identified (23 of them were susceptible of being quantified). The proposed methodology provided detection and quantification limits within the ranges of 0.004-7.143 g/mL and 0.013-23.810 g/mL, respectively. As far as the repeatability is concerned, the relative standard deviation values were below 0.43% for retention time, and 9.05% for peak area. The developed methodology was applied for the determination of phenolic compounds in ten VOOs, both monovarietals and blends. Secoiridoids were the most abundant fraction in all the samples, followed by simple phenolic alcohols, lignans, flavonoids, and phenolic acids (being the abundance order of the latter chemical classes logically depending on the variety and origin of the VOOs).Fil: Monasterio, Romina Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Olmo García, Lucía. Universidad de Granada. Facultad de Ciencias. Departamento de química analítica; EspañaFil: Bajoub, Aadil. Universidad de Granada. Facultad de Ciencias. Departamento de química analítica; EspañaFil: Fernández Gutiérrez, Alberto. Universidad de Granada. Facultad de Ciencias. Departamento de química analítica; EspañaFil: Carrasco Pancorbo, Alegría. Universidad de Granada. Facultad de Ciencias. Departamento de química analítica; EspañaMolecular Diversity Preservation International2016-09-24info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/23769Monasterio, Romina Paula; Olmo García, Lucía; Bajoub, Aadil; Fernández Gutiérrez, Alberto; Carrasco Pancorbo, Alegría; Potential of LC coupled to fluorescence detection in food metabolomics: Determination of phenolic compounds in virgin olive oil; Molecular Diversity Preservation International; International Journal of Molecular Sciences; 17; 10; 24-9-2016; 1-171422-0067CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.mdpi.com/1422-0067/17/10/1627info:eu-repo/semantics/altIdentifier/doi/10.3390/ijms17101627info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:05:18Zoai:ri.conicet.gov.ar:11336/23769instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:05:19.195CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Potential of LC coupled to fluorescence detection in food metabolomics: Determination of phenolic compounds in virgin olive oil |
title |
Potential of LC coupled to fluorescence detection in food metabolomics: Determination of phenolic compounds in virgin olive oil |
spellingShingle |
Potential of LC coupled to fluorescence detection in food metabolomics: Determination of phenolic compounds in virgin olive oil Monasterio, Romina Paula FLUORESCENCE DETECTION OLIVE OIL PHENOLIC COMPOUNDS SECOIRIDOIDS FOOD METABOLOMICS |
title_short |
Potential of LC coupled to fluorescence detection in food metabolomics: Determination of phenolic compounds in virgin olive oil |
title_full |
Potential of LC coupled to fluorescence detection in food metabolomics: Determination of phenolic compounds in virgin olive oil |
title_fullStr |
Potential of LC coupled to fluorescence detection in food metabolomics: Determination of phenolic compounds in virgin olive oil |
title_full_unstemmed |
Potential of LC coupled to fluorescence detection in food metabolomics: Determination of phenolic compounds in virgin olive oil |
title_sort |
Potential of LC coupled to fluorescence detection in food metabolomics: Determination of phenolic compounds in virgin olive oil |
dc.creator.none.fl_str_mv |
Monasterio, Romina Paula Olmo García, Lucía Bajoub, Aadil Fernández Gutiérrez, Alberto Carrasco Pancorbo, Alegría |
author |
Monasterio, Romina Paula |
author_facet |
Monasterio, Romina Paula Olmo García, Lucía Bajoub, Aadil Fernández Gutiérrez, Alberto Carrasco Pancorbo, Alegría |
author_role |
author |
author2 |
Olmo García, Lucía Bajoub, Aadil Fernández Gutiérrez, Alberto Carrasco Pancorbo, Alegría |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
FLUORESCENCE DETECTION OLIVE OIL PHENOLIC COMPOUNDS SECOIRIDOIDS FOOD METABOLOMICS |
topic |
FLUORESCENCE DETECTION OLIVE OIL PHENOLIC COMPOUNDS SECOIRIDOIDS FOOD METABOLOMICS |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.4 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
A powerful chromatographic method coupled to a fluorescence detector was developed to determine the phenolic compounds present in virgin olive oil (VOO), with the aim to propose an appropriate alternative to liquid chromatography-mass spectrometry. An excitation wavelength of 285 nm was selected and four different emission wavelengths (316, 328, 350 and 450 nm) were simultaneously recorded, working therefore on "multi-emission" detection mode. With the use of commercially available standards and other standards obtained by semipreparative high performance liquid chromatography, it was possible to identify simple phenols, lignans, several complex phenols, and other phenolic compounds present in the matrix under study. A total of 26 phenolic compounds belonging to different chemical families were identified (23 of them were susceptible of being quantified). The proposed methodology provided detection and quantification limits within the ranges of 0.004-7.143 g/mL and 0.013-23.810 g/mL, respectively. As far as the repeatability is concerned, the relative standard deviation values were below 0.43% for retention time, and 9.05% for peak area. The developed methodology was applied for the determination of phenolic compounds in ten VOOs, both monovarietals and blends. Secoiridoids were the most abundant fraction in all the samples, followed by simple phenolic alcohols, lignans, flavonoids, and phenolic acids (being the abundance order of the latter chemical classes logically depending on the variety and origin of the VOOs). Fil: Monasterio, Romina Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina Fil: Olmo García, Lucía. Universidad de Granada. Facultad de Ciencias. Departamento de química analítica; España Fil: Bajoub, Aadil. Universidad de Granada. Facultad de Ciencias. Departamento de química analítica; España Fil: Fernández Gutiérrez, Alberto. Universidad de Granada. Facultad de Ciencias. Departamento de química analítica; España Fil: Carrasco Pancorbo, Alegría. Universidad de Granada. Facultad de Ciencias. Departamento de química analítica; España |
description |
A powerful chromatographic method coupled to a fluorescence detector was developed to determine the phenolic compounds present in virgin olive oil (VOO), with the aim to propose an appropriate alternative to liquid chromatography-mass spectrometry. An excitation wavelength of 285 nm was selected and four different emission wavelengths (316, 328, 350 and 450 nm) were simultaneously recorded, working therefore on "multi-emission" detection mode. With the use of commercially available standards and other standards obtained by semipreparative high performance liquid chromatography, it was possible to identify simple phenols, lignans, several complex phenols, and other phenolic compounds present in the matrix under study. A total of 26 phenolic compounds belonging to different chemical families were identified (23 of them were susceptible of being quantified). The proposed methodology provided detection and quantification limits within the ranges of 0.004-7.143 g/mL and 0.013-23.810 g/mL, respectively. As far as the repeatability is concerned, the relative standard deviation values were below 0.43% for retention time, and 9.05% for peak area. The developed methodology was applied for the determination of phenolic compounds in ten VOOs, both monovarietals and blends. Secoiridoids were the most abundant fraction in all the samples, followed by simple phenolic alcohols, lignans, flavonoids, and phenolic acids (being the abundance order of the latter chemical classes logically depending on the variety and origin of the VOOs). |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-09-24 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/23769 Monasterio, Romina Paula; Olmo García, Lucía; Bajoub, Aadil; Fernández Gutiérrez, Alberto; Carrasco Pancorbo, Alegría; Potential of LC coupled to fluorescence detection in food metabolomics: Determination of phenolic compounds in virgin olive oil; Molecular Diversity Preservation International; International Journal of Molecular Sciences; 17; 10; 24-9-2016; 1-17 1422-0067 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/23769 |
identifier_str_mv |
Monasterio, Romina Paula; Olmo García, Lucía; Bajoub, Aadil; Fernández Gutiérrez, Alberto; Carrasco Pancorbo, Alegría; Potential of LC coupled to fluorescence detection in food metabolomics: Determination of phenolic compounds in virgin olive oil; Molecular Diversity Preservation International; International Journal of Molecular Sciences; 17; 10; 24-9-2016; 1-17 1422-0067 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://www.mdpi.com/1422-0067/17/10/1627 info:eu-repo/semantics/altIdentifier/doi/10.3390/ijms17101627 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Molecular Diversity Preservation International |
publisher.none.fl_str_mv |
Molecular Diversity Preservation International |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613888038928384 |
score |
13.070432 |