Metabolic profiling approach to determine phenolic compounds of virgin olive oil by direct injection and liquid chromatography coupled to mass spectrometry

Autores
Olmo García, Lucía; Bajoub, Aadil; Monasterio, Romina Paula; Fernandez-gutierrez, Alberto; Carrasco-pancorbo, Alegria
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A LC–MS method involving direct injection of extra-virgin olive oil (EVOO) – after a simple dilution – for determining its phenolic compounds has been developed. Optimization of the most appropriate solvent for sample dilution, selection of the optimum oil/solvent ratio, and establishment of column cleaning strategy and maximum number of injections were some of the most relevant steps. Then, the analytical parameters of the method were evaluated, establishing LOD (from 3.3 to 31.6 µg/L) and LOQ, precision (RSD values for inter-day repeatability were found between 3.49 and 6.12%), and trueness (within the range 89.9–102.3% for 1.0 mg/L) and checking possible matrix effect (which was no significant). Three kinds of calibration were used: external standard, standard addition and calibration in a phenols-free matrix, which was subsequently applied to quantify the phenolic compounds in 16 EVOOs (from 6 cultivars). A total of 21 compounds were determined without the need of using any extraction protocol.
Fil: Olmo García, Lucía. Universidad de Granada; España
Fil: Bajoub, Aadil. Universidad de Granada; España
Fil: Monasterio, Romina Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Fernandez-gutierrez, Alberto. Universidad de Granada; España
Fil: Carrasco-pancorbo, Alegria. Universidad de Granada; España
Materia
Direct Injection
Extra-Virgin Olive Oil
Liquid Chromatography&Ndash;Mass Spectrometry
Matrix Effect
Phenolic Compounds
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/40005
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Metabolic profiling approach to determine phenolic compounds of virgin olive oil by direct injection and liquid chromatography coupled to mass spectrometryOlmo García, LucíaBajoub, AadilMonasterio, Romina PaulaFernandez-gutierrez, AlbertoCarrasco-pancorbo, AlegriaDirect InjectionExtra-Virgin Olive OilLiquid Chromatography&Ndash;Mass SpectrometryMatrix EffectPhenolic Compoundshttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1A LC–MS method involving direct injection of extra-virgin olive oil (EVOO) – after a simple dilution – for determining its phenolic compounds has been developed. Optimization of the most appropriate solvent for sample dilution, selection of the optimum oil/solvent ratio, and establishment of column cleaning strategy and maximum number of injections were some of the most relevant steps. Then, the analytical parameters of the method were evaluated, establishing LOD (from 3.3 to 31.6 µg/L) and LOQ, precision (RSD values for inter-day repeatability were found between 3.49 and 6.12%), and trueness (within the range 89.9–102.3% for 1.0 mg/L) and checking possible matrix effect (which was no significant). Three kinds of calibration were used: external standard, standard addition and calibration in a phenols-free matrix, which was subsequently applied to quantify the phenolic compounds in 16 EVOOs (from 6 cultivars). A total of 21 compounds were determined without the need of using any extraction protocol.Fil: Olmo García, Lucía. Universidad de Granada; EspañaFil: Bajoub, Aadil. Universidad de Granada; EspañaFil: Monasterio, Romina Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Fernandez-gutierrez, Alberto. Universidad de Granada; EspañaFil: Carrasco-pancorbo, Alegria. Universidad de Granada; EspañaElsevier2017-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/40005Olmo García, Lucía; Bajoub, Aadil; Monasterio, Romina Paula; Fernandez-gutierrez, Alberto; Carrasco-pancorbo, Alegria; Metabolic profiling approach to determine phenolic compounds of virgin olive oil by direct injection and liquid chromatography coupled to mass spectrometry; Elsevier; Food Chemistry; 231; 9-2017; 374-3850308-8146CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.foodchem.2017.03.139info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0308814617305344info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:50:59Zoai:ri.conicet.gov.ar:11336/40005instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:50:59.616CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Metabolic profiling approach to determine phenolic compounds of virgin olive oil by direct injection and liquid chromatography coupled to mass spectrometry
title Metabolic profiling approach to determine phenolic compounds of virgin olive oil by direct injection and liquid chromatography coupled to mass spectrometry
spellingShingle Metabolic profiling approach to determine phenolic compounds of virgin olive oil by direct injection and liquid chromatography coupled to mass spectrometry
Olmo García, Lucía
Direct Injection
Extra-Virgin Olive Oil
Liquid Chromatography&Ndash;Mass Spectrometry
Matrix Effect
Phenolic Compounds
title_short Metabolic profiling approach to determine phenolic compounds of virgin olive oil by direct injection and liquid chromatography coupled to mass spectrometry
title_full Metabolic profiling approach to determine phenolic compounds of virgin olive oil by direct injection and liquid chromatography coupled to mass spectrometry
title_fullStr Metabolic profiling approach to determine phenolic compounds of virgin olive oil by direct injection and liquid chromatography coupled to mass spectrometry
title_full_unstemmed Metabolic profiling approach to determine phenolic compounds of virgin olive oil by direct injection and liquid chromatography coupled to mass spectrometry
title_sort Metabolic profiling approach to determine phenolic compounds of virgin olive oil by direct injection and liquid chromatography coupled to mass spectrometry
dc.creator.none.fl_str_mv Olmo García, Lucía
Bajoub, Aadil
Monasterio, Romina Paula
Fernandez-gutierrez, Alberto
Carrasco-pancorbo, Alegria
author Olmo García, Lucía
author_facet Olmo García, Lucía
Bajoub, Aadil
Monasterio, Romina Paula
Fernandez-gutierrez, Alberto
Carrasco-pancorbo, Alegria
author_role author
author2 Bajoub, Aadil
Monasterio, Romina Paula
Fernandez-gutierrez, Alberto
Carrasco-pancorbo, Alegria
author2_role author
author
author
author
dc.subject.none.fl_str_mv Direct Injection
Extra-Virgin Olive Oil
Liquid Chromatography&Ndash;Mass Spectrometry
Matrix Effect
Phenolic Compounds
topic Direct Injection
Extra-Virgin Olive Oil
Liquid Chromatography&Ndash;Mass Spectrometry
Matrix Effect
Phenolic Compounds
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A LC–MS method involving direct injection of extra-virgin olive oil (EVOO) – after a simple dilution – for determining its phenolic compounds has been developed. Optimization of the most appropriate solvent for sample dilution, selection of the optimum oil/solvent ratio, and establishment of column cleaning strategy and maximum number of injections were some of the most relevant steps. Then, the analytical parameters of the method were evaluated, establishing LOD (from 3.3 to 31.6 µg/L) and LOQ, precision (RSD values for inter-day repeatability were found between 3.49 and 6.12%), and trueness (within the range 89.9–102.3% for 1.0 mg/L) and checking possible matrix effect (which was no significant). Three kinds of calibration were used: external standard, standard addition and calibration in a phenols-free matrix, which was subsequently applied to quantify the phenolic compounds in 16 EVOOs (from 6 cultivars). A total of 21 compounds were determined without the need of using any extraction protocol.
Fil: Olmo García, Lucía. Universidad de Granada; España
Fil: Bajoub, Aadil. Universidad de Granada; España
Fil: Monasterio, Romina Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Fernandez-gutierrez, Alberto. Universidad de Granada; España
Fil: Carrasco-pancorbo, Alegria. Universidad de Granada; España
description A LC–MS method involving direct injection of extra-virgin olive oil (EVOO) – after a simple dilution – for determining its phenolic compounds has been developed. Optimization of the most appropriate solvent for sample dilution, selection of the optimum oil/solvent ratio, and establishment of column cleaning strategy and maximum number of injections were some of the most relevant steps. Then, the analytical parameters of the method were evaluated, establishing LOD (from 3.3 to 31.6 µg/L) and LOQ, precision (RSD values for inter-day repeatability were found between 3.49 and 6.12%), and trueness (within the range 89.9–102.3% for 1.0 mg/L) and checking possible matrix effect (which was no significant). Three kinds of calibration were used: external standard, standard addition and calibration in a phenols-free matrix, which was subsequently applied to quantify the phenolic compounds in 16 EVOOs (from 6 cultivars). A total of 21 compounds were determined without the need of using any extraction protocol.
publishDate 2017
dc.date.none.fl_str_mv 2017-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/40005
Olmo García, Lucía; Bajoub, Aadil; Monasterio, Romina Paula; Fernandez-gutierrez, Alberto; Carrasco-pancorbo, Alegria; Metabolic profiling approach to determine phenolic compounds of virgin olive oil by direct injection and liquid chromatography coupled to mass spectrometry; Elsevier; Food Chemistry; 231; 9-2017; 374-385
0308-8146
CONICET Digital
CONICET
url http://hdl.handle.net/11336/40005
identifier_str_mv Olmo García, Lucía; Bajoub, Aadil; Monasterio, Romina Paula; Fernandez-gutierrez, Alberto; Carrasco-pancorbo, Alegria; Metabolic profiling approach to determine phenolic compounds of virgin olive oil by direct injection and liquid chromatography coupled to mass spectrometry; Elsevier; Food Chemistry; 231; 9-2017; 374-385
0308-8146
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.foodchem.2017.03.139
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0308814617305344
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613569654554624
score 13.070432

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