Herpes simplex virus type 1 variants arising after selection with an antiviral carrageenan: Lack of correlation between drug susceptibility and syn phenotype

Autores
Carlucci, Maria Josefina; Scolaro, Luis Alberto; Damonte, Elsa Beatriz
Año de publicación
2002
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Natural carrageenans of diverse structural types isolated from the red seaweed Gigartina skottsbergii have been recently identified as potent and selective inhibitors of herpes simplex virus (HSV) types 1 and 2. The m/n carrageenan 1C3  was tested in vitro for its ability to select resistant variants. After serial passages of HSV-1 strain F in Vero cells in the presence of increasing concentrations of 1C3, there emerged viruses which were approximately 2-10 fold more resistant to 1C3 inhibition than parental virus and formed large plaques with an altered syncytial phenotype (1C3-syn). Plaque-purified syncytial variants isolated from passages 13 and 14 have shown variable levels of resistance to 1C3 as well as to the other antiviral carrageenans isolated from G. skottsbergii, and to other sulfated polysaccharides with known antiviral activity, such as heparin and dextran sulfate 8000, but all the clones were susceptible to acyclovir. The syn phenotype was not related to polysaccharide-resistance. All the 1C3-syn variants formed large syncytia in Vero and CV-1 cells, but did not induce fusion in other cell types. The growth efficiency in Vero cells as well as the virulence for mice by intracerebral or intraperitoneal inoculation of 1C3-syn variants showed no significative alterations in comparison to the parental virus. The syncytial properties were not affected by cyclosporin A or melittin, suggesting that an alteration on glycoprotein gB could be responsible of the syn phenotype induced by 1C3.
Fil: Carlucci, Maria Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina
Fil: Scolaro, Luis Alberto. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Damonte, Elsa Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina
Materia
herpes simplex virus
carrageenan
viral variant
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/102827
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/102827
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Herpes simplex virus type 1 variants arising after selection with an antiviral carrageenan: Lack of correlation between drug susceptibility and syn phenotypeCarlucci, Maria JosefinaScolaro, Luis AlbertoDamonte, Elsa Beatrizherpes simplex viruscarrageenanviral varianthttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Natural carrageenans of diverse structural types isolated from the red seaweed Gigartina skottsbergii have been recently identified as potent and selective inhibitors of herpes simplex virus (HSV) types 1 and 2. The m/n carrageenan 1C3  was tested in vitro for its ability to select resistant variants. After serial passages of HSV-1 strain F in Vero cells in the presence of increasing concentrations of 1C3, there emerged viruses which were approximately 2-10 fold more resistant to 1C3 inhibition than parental virus and formed large plaques with an altered syncytial phenotype (1C3-syn). Plaque-purified syncytial variants isolated from passages 13 and 14 have shown variable levels of resistance to 1C3 as well as to the other antiviral carrageenans isolated from G. skottsbergii, and to other sulfated polysaccharides with known antiviral activity, such as heparin and dextran sulfate 8000, but all the clones were susceptible to acyclovir. The syn phenotype was not related to polysaccharide-resistance. All the 1C3-syn variants formed large syncytia in Vero and CV-1 cells, but did not induce fusion in other cell types. The growth efficiency in Vero cells as well as the virulence for mice by intracerebral or intraperitoneal inoculation of 1C3-syn variants showed no significative alterations in comparison to the parental virus. The syncytial properties were not affected by cyclosporin A or melittin, suggesting that an alteration on glycoprotein gB could be responsible of the syn phenotype induced by 1C3.Fil: Carlucci, Maria Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; ArgentinaFil: Scolaro, Luis Alberto. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Damonte, Elsa Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; ArgentinaWiley-liss, Div John Wiley & Sons Inc2002-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/mswordapplication/mswordapplication/pdfhttp://hdl.handle.net/11336/102827Carlucci, Maria Josefina; Scolaro, Luis Alberto; Damonte, Elsa Beatriz; Herpes simplex virus type 1 variants arising after selection with an antiviral carrageenan: Lack of correlation between drug susceptibility and syn phenotype; Wiley-liss, Div John Wiley & Sons Inc; Journal of Medical Virology; 68; 1; 9-2002; 92-980146-6615CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/jmv.10174info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/jmv.10174info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:56:00Zoai:ri.conicet.gov.ar:11336/102827instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:56:00.496CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Herpes simplex virus type 1 variants arising after selection with an antiviral carrageenan: Lack of correlation between drug susceptibility and syn phenotype
title Herpes simplex virus type 1 variants arising after selection with an antiviral carrageenan: Lack of correlation between drug susceptibility and syn phenotype
spellingShingle Herpes simplex virus type 1 variants arising after selection with an antiviral carrageenan: Lack of correlation between drug susceptibility and syn phenotype
Carlucci, Maria Josefina
herpes simplex virus
carrageenan
viral variant
title_short Herpes simplex virus type 1 variants arising after selection with an antiviral carrageenan: Lack of correlation between drug susceptibility and syn phenotype
title_full Herpes simplex virus type 1 variants arising after selection with an antiviral carrageenan: Lack of correlation between drug susceptibility and syn phenotype
title_fullStr Herpes simplex virus type 1 variants arising after selection with an antiviral carrageenan: Lack of correlation between drug susceptibility and syn phenotype
title_full_unstemmed Herpes simplex virus type 1 variants arising after selection with an antiviral carrageenan: Lack of correlation between drug susceptibility and syn phenotype
title_sort Herpes simplex virus type 1 variants arising after selection with an antiviral carrageenan: Lack of correlation between drug susceptibility and syn phenotype
dc.creator.none.fl_str_mv Carlucci, Maria Josefina
Scolaro, Luis Alberto
Damonte, Elsa Beatriz
author Carlucci, Maria Josefina
author_facet Carlucci, Maria Josefina
Scolaro, Luis Alberto
Damonte, Elsa Beatriz
author_role author
author2 Scolaro, Luis Alberto
Damonte, Elsa Beatriz
author2_role author
author
dc.subject.none.fl_str_mv herpes simplex virus
carrageenan
viral variant
topic herpes simplex virus
carrageenan
viral variant
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Natural carrageenans of diverse structural types isolated from the red seaweed Gigartina skottsbergii have been recently identified as potent and selective inhibitors of herpes simplex virus (HSV) types 1 and 2. The m/n carrageenan 1C3  was tested in vitro for its ability to select resistant variants. After serial passages of HSV-1 strain F in Vero cells in the presence of increasing concentrations of 1C3, there emerged viruses which were approximately 2-10 fold more resistant to 1C3 inhibition than parental virus and formed large plaques with an altered syncytial phenotype (1C3-syn). Plaque-purified syncytial variants isolated from passages 13 and 14 have shown variable levels of resistance to 1C3 as well as to the other antiviral carrageenans isolated from G. skottsbergii, and to other sulfated polysaccharides with known antiviral activity, such as heparin and dextran sulfate 8000, but all the clones were susceptible to acyclovir. The syn phenotype was not related to polysaccharide-resistance. All the 1C3-syn variants formed large syncytia in Vero and CV-1 cells, but did not induce fusion in other cell types. The growth efficiency in Vero cells as well as the virulence for mice by intracerebral or intraperitoneal inoculation of 1C3-syn variants showed no significative alterations in comparison to the parental virus. The syncytial properties were not affected by cyclosporin A or melittin, suggesting that an alteration on glycoprotein gB could be responsible of the syn phenotype induced by 1C3.
Fil: Carlucci, Maria Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina
Fil: Scolaro, Luis Alberto. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Damonte, Elsa Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Virología; Argentina
description Natural carrageenans of diverse structural types isolated from the red seaweed Gigartina skottsbergii have been recently identified as potent and selective inhibitors of herpes simplex virus (HSV) types 1 and 2. The m/n carrageenan 1C3  was tested in vitro for its ability to select resistant variants. After serial passages of HSV-1 strain F in Vero cells in the presence of increasing concentrations of 1C3, there emerged viruses which were approximately 2-10 fold more resistant to 1C3 inhibition than parental virus and formed large plaques with an altered syncytial phenotype (1C3-syn). Plaque-purified syncytial variants isolated from passages 13 and 14 have shown variable levels of resistance to 1C3 as well as to the other antiviral carrageenans isolated from G. skottsbergii, and to other sulfated polysaccharides with known antiviral activity, such as heparin and dextran sulfate 8000, but all the clones were susceptible to acyclovir. The syn phenotype was not related to polysaccharide-resistance. All the 1C3-syn variants formed large syncytia in Vero and CV-1 cells, but did not induce fusion in other cell types. The growth efficiency in Vero cells as well as the virulence for mice by intracerebral or intraperitoneal inoculation of 1C3-syn variants showed no significative alterations in comparison to the parental virus. The syncytial properties were not affected by cyclosporin A or melittin, suggesting that an alteration on glycoprotein gB could be responsible of the syn phenotype induced by 1C3.
publishDate 2002
dc.date.none.fl_str_mv 2002-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/102827
Carlucci, Maria Josefina; Scolaro, Luis Alberto; Damonte, Elsa Beatriz; Herpes simplex virus type 1 variants arising after selection with an antiviral carrageenan: Lack of correlation between drug susceptibility and syn phenotype; Wiley-liss, Div John Wiley & Sons Inc; Journal of Medical Virology; 68; 1; 9-2002; 92-98
0146-6615
CONICET Digital
CONICET
url http://hdl.handle.net/11336/102827
identifier_str_mv Carlucci, Maria Josefina; Scolaro, Luis Alberto; Damonte, Elsa Beatriz; Herpes simplex virus type 1 variants arising after selection with an antiviral carrageenan: Lack of correlation between drug susceptibility and syn phenotype; Wiley-liss, Div John Wiley & Sons Inc; Journal of Medical Virology; 68; 1; 9-2002; 92-98
0146-6615
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/jmv.10174
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/jmv.10174
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/msword
application/msword
application/pdf
dc.publisher.none.fl_str_mv Wiley-liss, Div John Wiley & Sons Inc
publisher.none.fl_str_mv Wiley-liss, Div John Wiley & Sons Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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