Cervicovaginal secretions contribute to innate resistance to herpes simplex virus infection

Autores
John, Minnie; Keller, Marla J.; Fam, Ehsan H.; Cheshenko, Natalia; Hogarty, Kathleen; Kosowitz, Andrea; Wallenstein, Sylvan; Carlucci, Maria Josefina; Tuyama, Ana C.; Lu, Wuyyan; Klotman, Mary E.; Lehrer, Robert I.; Herold, Betsy C.
Año de publicación
2005
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Defining and preserving the innate antiviral activity found in cervicovaginal secretions is critical. Cervicovaginal lavage (CVL) samples were obtained from 20 healthy women and evaluated for anti-herpes simplex virus (HSV) activity. CVL samples reduced HSV-2 yields by 23-fold (median), and the anti-HSV activity of CVL samples correlated with the concentration of human neutrophil peptides (HNP)-1-3. Both CVL samples and HNP-1-3 interacted with virus and prevented entry after binding. Substantially less protective activity was observed in CVL samples obtained from 20 human immunodeficiency virus-infected subjects, but the addition of CVL samples from healthy subjects enhanced the antiviral activity. The significance of the innate activity was further demonstrated by showing that CVL samples prevented murine genital herpes. Fourteen of 15 mice were protected from genital herpes if they were challenged with HSV-2 pretreated with CVL samples from healthy subjects. In contrast, all 15 mice challenged with untreated HSV-2 died. These findings are evidence that cervicovaginal secretions contribute to innate resistance to HSV-2 and identify defensins as contributors to this activity.
Fil: John, Minnie. Mount Sinai School of Medicine; Estados Unidos
Fil: Keller, Marla J.. Mount Sinai School of Medicine; Estados Unidos
Fil: Fam, Ehsan H.. Mount Sinai School of Medicine; Estados Unidos
Fil: Cheshenko, Natalia. Mount Sinai School of Medicine; Estados Unidos
Fil: Hogarty, Kathleen. Mount Sinai School of Medicine; Estados Unidos
Fil: Kosowitz, Andrea. Mount Sinai School of Medicine; Estados Unidos
Fil: Wallenstein, Sylvan. Mount Sinai School of Medicine; Estados Unidos
Fil: Carlucci, Maria Josefina. Mount Sinai School of Medicine; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Tuyama, Ana C.. Mount Sinai School of Medicine; Estados Unidos
Fil: Lu, Wuyyan. University of Maryland; Estados Unidos
Fil: Klotman, Mary E.. Mount Sinai School of Medicine; Estados Unidos
Fil: Lehrer, Robert I.. University of California at Los Angeles; Estados Unidos
Fil: Herold, Betsy C.. Mount Sinai School of Medicine; Estados Unidos
Materia
HERPES SIMPLEX VIRUS
CERVICOVAGINAL SECRETIONS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/97785
Acceder
id CONICETDig_fdb5a47818f61117d9e8ef2b36ace862
oai_identifier_str oai:ri.conicet.gov.ar:11336/97785
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Cervicovaginal secretions contribute to innate resistance to herpes simplex virus infectionJohn, MinnieKeller, Marla J.Fam, Ehsan H.Cheshenko, NataliaHogarty, KathleenKosowitz, AndreaWallenstein, SylvanCarlucci, Maria JosefinaTuyama, Ana C.Lu, WuyyanKlotman, Mary E.Lehrer, Robert I.Herold, Betsy C.HERPES SIMPLEX VIRUSCERVICOVAGINAL SECRETIONShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Defining and preserving the innate antiviral activity found in cervicovaginal secretions is critical. Cervicovaginal lavage (CVL) samples were obtained from 20 healthy women and evaluated for anti-herpes simplex virus (HSV) activity. CVL samples reduced HSV-2 yields by 23-fold (median), and the anti-HSV activity of CVL samples correlated with the concentration of human neutrophil peptides (HNP)-1-3. Both CVL samples and HNP-1-3 interacted with virus and prevented entry after binding. Substantially less protective activity was observed in CVL samples obtained from 20 human immunodeficiency virus-infected subjects, but the addition of CVL samples from healthy subjects enhanced the antiviral activity. The significance of the innate activity was further demonstrated by showing that CVL samples prevented murine genital herpes. Fourteen of 15 mice were protected from genital herpes if they were challenged with HSV-2 pretreated with CVL samples from healthy subjects. In contrast, all 15 mice challenged with untreated HSV-2 died. These findings are evidence that cervicovaginal secretions contribute to innate resistance to HSV-2 and identify defensins as contributors to this activity.Fil: John, Minnie. Mount Sinai School of Medicine; Estados UnidosFil: Keller, Marla J.. Mount Sinai School of Medicine; Estados UnidosFil: Fam, Ehsan H.. Mount Sinai School of Medicine; Estados UnidosFil: Cheshenko, Natalia. Mount Sinai School of Medicine; Estados UnidosFil: Hogarty, Kathleen. Mount Sinai School of Medicine; Estados UnidosFil: Kosowitz, Andrea. Mount Sinai School of Medicine; Estados UnidosFil: Wallenstein, Sylvan. Mount Sinai School of Medicine; Estados UnidosFil: Carlucci, Maria Josefina. Mount Sinai School of Medicine; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Tuyama, Ana C.. Mount Sinai School of Medicine; Estados UnidosFil: Lu, Wuyyan. University of Maryland; Estados UnidosFil: Klotman, Mary E.. Mount Sinai School of Medicine; Estados UnidosFil: Lehrer, Robert I.. University of California at Los Angeles; Estados UnidosFil: Herold, Betsy C.. Mount Sinai School of Medicine; Estados UnidosOxford University Press2005-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/97785John, Minnie; Keller, Marla J.; Fam, Ehsan H.; Cheshenko, Natalia; Hogarty, Kathleen; et al.; Cervicovaginal secretions contribute to innate resistance to herpes simplex virus infection; Oxford University Press; Journal Of Infectious Diseases; 192; 10; 11-2005; 1731-17400022-1899CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jid/article/192/10/1731/876451info:eu-repo/semantics/altIdentifier/doi/10.1086/497168info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:07:58Zoai:ri.conicet.gov.ar:11336/97785instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:07:59.162CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Cervicovaginal secretions contribute to innate resistance to herpes simplex virus infection
title Cervicovaginal secretions contribute to innate resistance to herpes simplex virus infection
spellingShingle Cervicovaginal secretions contribute to innate resistance to herpes simplex virus infection
John, Minnie
HERPES SIMPLEX VIRUS
CERVICOVAGINAL SECRETIONS
title_short Cervicovaginal secretions contribute to innate resistance to herpes simplex virus infection
title_full Cervicovaginal secretions contribute to innate resistance to herpes simplex virus infection
title_fullStr Cervicovaginal secretions contribute to innate resistance to herpes simplex virus infection
title_full_unstemmed Cervicovaginal secretions contribute to innate resistance to herpes simplex virus infection
title_sort Cervicovaginal secretions contribute to innate resistance to herpes simplex virus infection
dc.creator.none.fl_str_mv John, Minnie
Keller, Marla J.
Fam, Ehsan H.
Cheshenko, Natalia
Hogarty, Kathleen
Kosowitz, Andrea
Wallenstein, Sylvan
Carlucci, Maria Josefina
Tuyama, Ana C.
Lu, Wuyyan
Klotman, Mary E.
Lehrer, Robert I.
Herold, Betsy C.
author John, Minnie
author_facet John, Minnie
Keller, Marla J.
Fam, Ehsan H.
Cheshenko, Natalia
Hogarty, Kathleen
Kosowitz, Andrea
Wallenstein, Sylvan
Carlucci, Maria Josefina
Tuyama, Ana C.
Lu, Wuyyan
Klotman, Mary E.
Lehrer, Robert I.
Herold, Betsy C.
author_role author
author2 Keller, Marla J.
Fam, Ehsan H.
Cheshenko, Natalia
Hogarty, Kathleen
Kosowitz, Andrea
Wallenstein, Sylvan
Carlucci, Maria Josefina
Tuyama, Ana C.
Lu, Wuyyan
Klotman, Mary E.
Lehrer, Robert I.
Herold, Betsy C.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv HERPES SIMPLEX VIRUS
CERVICOVAGINAL SECRETIONS
topic HERPES SIMPLEX VIRUS
CERVICOVAGINAL SECRETIONS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Defining and preserving the innate antiviral activity found in cervicovaginal secretions is critical. Cervicovaginal lavage (CVL) samples were obtained from 20 healthy women and evaluated for anti-herpes simplex virus (HSV) activity. CVL samples reduced HSV-2 yields by 23-fold (median), and the anti-HSV activity of CVL samples correlated with the concentration of human neutrophil peptides (HNP)-1-3. Both CVL samples and HNP-1-3 interacted with virus and prevented entry after binding. Substantially less protective activity was observed in CVL samples obtained from 20 human immunodeficiency virus-infected subjects, but the addition of CVL samples from healthy subjects enhanced the antiviral activity. The significance of the innate activity was further demonstrated by showing that CVL samples prevented murine genital herpes. Fourteen of 15 mice were protected from genital herpes if they were challenged with HSV-2 pretreated with CVL samples from healthy subjects. In contrast, all 15 mice challenged with untreated HSV-2 died. These findings are evidence that cervicovaginal secretions contribute to innate resistance to HSV-2 and identify defensins as contributors to this activity.
Fil: John, Minnie. Mount Sinai School of Medicine; Estados Unidos
Fil: Keller, Marla J.. Mount Sinai School of Medicine; Estados Unidos
Fil: Fam, Ehsan H.. Mount Sinai School of Medicine; Estados Unidos
Fil: Cheshenko, Natalia. Mount Sinai School of Medicine; Estados Unidos
Fil: Hogarty, Kathleen. Mount Sinai School of Medicine; Estados Unidos
Fil: Kosowitz, Andrea. Mount Sinai School of Medicine; Estados Unidos
Fil: Wallenstein, Sylvan. Mount Sinai School of Medicine; Estados Unidos
Fil: Carlucci, Maria Josefina. Mount Sinai School of Medicine; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Tuyama, Ana C.. Mount Sinai School of Medicine; Estados Unidos
Fil: Lu, Wuyyan. University of Maryland; Estados Unidos
Fil: Klotman, Mary E.. Mount Sinai School of Medicine; Estados Unidos
Fil: Lehrer, Robert I.. University of California at Los Angeles; Estados Unidos
Fil: Herold, Betsy C.. Mount Sinai School of Medicine; Estados Unidos
description Defining and preserving the innate antiviral activity found in cervicovaginal secretions is critical. Cervicovaginal lavage (CVL) samples were obtained from 20 healthy women and evaluated for anti-herpes simplex virus (HSV) activity. CVL samples reduced HSV-2 yields by 23-fold (median), and the anti-HSV activity of CVL samples correlated with the concentration of human neutrophil peptides (HNP)-1-3. Both CVL samples and HNP-1-3 interacted with virus and prevented entry after binding. Substantially less protective activity was observed in CVL samples obtained from 20 human immunodeficiency virus-infected subjects, but the addition of CVL samples from healthy subjects enhanced the antiviral activity. The significance of the innate activity was further demonstrated by showing that CVL samples prevented murine genital herpes. Fourteen of 15 mice were protected from genital herpes if they were challenged with HSV-2 pretreated with CVL samples from healthy subjects. In contrast, all 15 mice challenged with untreated HSV-2 died. These findings are evidence that cervicovaginal secretions contribute to innate resistance to HSV-2 and identify defensins as contributors to this activity.
publishDate 2005
dc.date.none.fl_str_mv 2005-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/97785
John, Minnie; Keller, Marla J.; Fam, Ehsan H.; Cheshenko, Natalia; Hogarty, Kathleen; et al.; Cervicovaginal secretions contribute to innate resistance to herpes simplex virus infection; Oxford University Press; Journal Of Infectious Diseases; 192; 10; 11-2005; 1731-1740
0022-1899
CONICET Digital
CONICET
url http://hdl.handle.net/11336/97785
identifier_str_mv John, Minnie; Keller, Marla J.; Fam, Ehsan H.; Cheshenko, Natalia; Hogarty, Kathleen; et al.; Cervicovaginal secretions contribute to innate resistance to herpes simplex virus infection; Oxford University Press; Journal Of Infectious Diseases; 192; 10; 11-2005; 1731-1740
0022-1899
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jid/article/192/10/1731/876451
info:eu-repo/semantics/altIdentifier/doi/10.1086/497168
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842270026190553088
score 13.13397

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