Biofilm producing lactic acid bacterial as an alternative to control food contaminations

Autores
Merino, Lina Ethel; Trejo, Fernando Miguel; de Antoni, Graciela Liliana; Golowczyc, Marina Alejandra
Año de publicación
2019
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Biofilms are complex structures of microorganisms that colonize various biotic or abiotic surfaces. These organized communities are formed by bacteria embedded in a highly hydrated extracellular matrix that is composed mainly of polysaccharides, proteins, DNA and other substances. Several bacterial surface structures are necessary for this surface adhesion and an environment that favors the formation of biofilm against planktonic growth is required. The presence of biofilms is common in food industry and represents a concern because bacteria can adhere to almost any type of surface,such as plastic, metal, glass, soil particles and wood. Lactic bacteria are generally recognized as safe (GRAS) and could be an alternative for the biocontrol of pathogenic microorganisms biofilms forming in the food production chain. Use of probiotic biofilms can be an alternative approach for reducing the formation of pathogenic biofilms in food industries.The objective of this work was to study the biofilm production of lactic acid bacteria in different substrates and in different surfaces such as plastic, glass and stainless steel, commonly used in food industry. Biofilm formation was measured by violet crystal and plaque counts of seven strains of lactobacilli: Lb. kefiri CIDCA 83113, CIDCA 8321, CIDCA 8344 and CIDCA 5818, Lb. plantarum CIDCA 83114 and CIDCA 8327 and Lb. delbrueckii subsp. lactis CIDCA 133. These have different characteristics such as presence of S-layer, exopolysaccharide or glucan production, autoaggregation and hydrophobicity.The percentage of autoaggregation by decreasing OD at 600 nm and the percentage of hydrophobicity by partition in hexadecane and xylene (MATH method) was measured. For measurement of biofilmformation, 1 ml of medium (MRS and BHI) was inoculated with lactobacilli in 24 wells culture plates.For viable counts, the adhered bacteria were resuspended in PBS and counted in MRS agar plates.Only Lb. kefiri CIDCA 8321 and CIDCA 8344 strains presented a percentage of autoaggregationgreater than 50%. Both strains also showed the highest hydrophobicity. None of the lactobacilli tested form biofilm in BHI medium. On the other hand, except for CIDCA 8321 strain that did not develop biofilm on any probed condition, the lactobacilli can form biofilm in MRS medium. The lowest biofilm production was observed in plastic, whereas in glass and stainless steel lactobacilli present high capacity of biofilm formation. It is remarkable that Lb. plantarum CIDCA 83114 is the one with the highest capacity for biofilm formation in the three surfaces studied, being stainless steel the most suitable surface for biofilm production. We observed that both hydrophobic and hydrophilic strains are capable of producing biofilm. We demonstrated that lactobacilli can form biofilm and this property depends on surface and growth media. This could be used as an alternative control of food pathogens.
Fil: Merino, Lina Ethel. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Fil: Trejo, Fernando Miguel. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Fil: de Antoni, Graciela Liliana. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Fil: Golowczyc, Marina Alejandra. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
XII Congreso Argentino de Microbiología General
San Miguel de Tucuman
Argentina
Asociación Civil de Microbiología General
Materia
BIOFILMS
BIOFILMS
LACTOBACILLI
LACTOBACILLI
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/168455
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/168455
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network_name_str CONICET Digital (CONICET)
spelling Biofilm producing lactic acid bacterial as an alternative to control food contaminationsMerino, Lina EthelTrejo, Fernando Miguelde Antoni, Graciela LilianaGolowczyc, Marina AlejandraBIOFILMSBIOFILMSLACTOBACILLILACTOBACILLIhttps://purl.org/becyt/ford/1.7https://purl.org/becyt/ford/1Biofilms are complex structures of microorganisms that colonize various biotic or abiotic surfaces. These organized communities are formed by bacteria embedded in a highly hydrated extracellular matrix that is composed mainly of polysaccharides, proteins, DNA and other substances. Several bacterial surface structures are necessary for this surface adhesion and an environment that favors the formation of biofilm against planktonic growth is required. The presence of biofilms is common in food industry and represents a concern because bacteria can adhere to almost any type of surface,such as plastic, metal, glass, soil particles and wood. Lactic bacteria are generally recognized as safe (GRAS) and could be an alternative for the biocontrol of pathogenic microorganisms biofilms forming in the food production chain. Use of probiotic biofilms can be an alternative approach for reducing the formation of pathogenic biofilms in food industries.The objective of this work was to study the biofilm production of lactic acid bacteria in different substrates and in different surfaces such as plastic, glass and stainless steel, commonly used in food industry. Biofilm formation was measured by violet crystal and plaque counts of seven strains of lactobacilli: Lb. kefiri CIDCA 83113, CIDCA 8321, CIDCA 8344 and CIDCA 5818, Lb. plantarum CIDCA 83114 and CIDCA 8327 and Lb. delbrueckii subsp. lactis CIDCA 133. These have different characteristics such as presence of S-layer, exopolysaccharide or glucan production, autoaggregation and hydrophobicity.The percentage of autoaggregation by decreasing OD at 600 nm and the percentage of hydrophobicity by partition in hexadecane and xylene (MATH method) was measured. For measurement of biofilmformation, 1 ml of medium (MRS and BHI) was inoculated with lactobacilli in 24 wells culture plates.For viable counts, the adhered bacteria were resuspended in PBS and counted in MRS agar plates.Only Lb. kefiri CIDCA 8321 and CIDCA 8344 strains presented a percentage of autoaggregationgreater than 50%. Both strains also showed the highest hydrophobicity. None of the lactobacilli tested form biofilm in BHI medium. On the other hand, except for CIDCA 8321 strain that did not develop biofilm on any probed condition, the lactobacilli can form biofilm in MRS medium. The lowest biofilm production was observed in plastic, whereas in glass and stainless steel lactobacilli present high capacity of biofilm formation. It is remarkable that Lb. plantarum CIDCA 83114 is the one with the highest capacity for biofilm formation in the three surfaces studied, being stainless steel the most suitable surface for biofilm production. We observed that both hydrophobic and hydrophilic strains are capable of producing biofilm. We demonstrated that lactobacilli can form biofilm and this property depends on surface and growth media. This could be used as an alternative control of food pathogens.Fil: Merino, Lina Ethel. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; ArgentinaFil: Trejo, Fernando Miguel. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; ArgentinaFil: de Antoni, Graciela Liliana. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; ArgentinaFil: Golowczyc, Marina Alejandra. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; ArgentinaXII Congreso Argentino de Microbiología GeneralSan Miguel de TucumanArgentinaAsociación Civil de Microbiología GeneralAsociación Civil de Microbiología General2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciatext/plaintext/richtextapplication/pdfhttp://hdl.handle.net/11336/168455Biofilm producing lactic acid bacterial as an alternative to control food contaminations; XII Congreso Argentino de Microbiología General; San Miguel de Tucuman; Argentina; 2017; 1-3CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.samige.org.ar/admin/news/files/108-Libro%20SAMIGE%202017.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:18:34Zoai:ri.conicet.gov.ar:11336/168455instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:18:34.298CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Biofilm producing lactic acid bacterial as an alternative to control food contaminations
title Biofilm producing lactic acid bacterial as an alternative to control food contaminations
spellingShingle Biofilm producing lactic acid bacterial as an alternative to control food contaminations
Merino, Lina Ethel
BIOFILMS
BIOFILMS
LACTOBACILLI
LACTOBACILLI
title_short Biofilm producing lactic acid bacterial as an alternative to control food contaminations
title_full Biofilm producing lactic acid bacterial as an alternative to control food contaminations
title_fullStr Biofilm producing lactic acid bacterial as an alternative to control food contaminations
title_full_unstemmed Biofilm producing lactic acid bacterial as an alternative to control food contaminations
title_sort Biofilm producing lactic acid bacterial as an alternative to control food contaminations
dc.creator.none.fl_str_mv Merino, Lina Ethel
Trejo, Fernando Miguel
de Antoni, Graciela Liliana
Golowczyc, Marina Alejandra
author Merino, Lina Ethel
author_facet Merino, Lina Ethel
Trejo, Fernando Miguel
de Antoni, Graciela Liliana
Golowczyc, Marina Alejandra
author_role author
author2 Trejo, Fernando Miguel
de Antoni, Graciela Liliana
Golowczyc, Marina Alejandra
author2_role author
author
author
dc.subject.none.fl_str_mv BIOFILMS
BIOFILMS
LACTOBACILLI
LACTOBACILLI
topic BIOFILMS
BIOFILMS
LACTOBACILLI
LACTOBACILLI
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.7
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Biofilms are complex structures of microorganisms that colonize various biotic or abiotic surfaces. These organized communities are formed by bacteria embedded in a highly hydrated extracellular matrix that is composed mainly of polysaccharides, proteins, DNA and other substances. Several bacterial surface structures are necessary for this surface adhesion and an environment that favors the formation of biofilm against planktonic growth is required. The presence of biofilms is common in food industry and represents a concern because bacteria can adhere to almost any type of surface,such as plastic, metal, glass, soil particles and wood. Lactic bacteria are generally recognized as safe (GRAS) and could be an alternative for the biocontrol of pathogenic microorganisms biofilms forming in the food production chain. Use of probiotic biofilms can be an alternative approach for reducing the formation of pathogenic biofilms in food industries.The objective of this work was to study the biofilm production of lactic acid bacteria in different substrates and in different surfaces such as plastic, glass and stainless steel, commonly used in food industry. Biofilm formation was measured by violet crystal and plaque counts of seven strains of lactobacilli: Lb. kefiri CIDCA 83113, CIDCA 8321, CIDCA 8344 and CIDCA 5818, Lb. plantarum CIDCA 83114 and CIDCA 8327 and Lb. delbrueckii subsp. lactis CIDCA 133. These have different characteristics such as presence of S-layer, exopolysaccharide or glucan production, autoaggregation and hydrophobicity.The percentage of autoaggregation by decreasing OD at 600 nm and the percentage of hydrophobicity by partition in hexadecane and xylene (MATH method) was measured. For measurement of biofilmformation, 1 ml of medium (MRS and BHI) was inoculated with lactobacilli in 24 wells culture plates.For viable counts, the adhered bacteria were resuspended in PBS and counted in MRS agar plates.Only Lb. kefiri CIDCA 8321 and CIDCA 8344 strains presented a percentage of autoaggregationgreater than 50%. Both strains also showed the highest hydrophobicity. None of the lactobacilli tested form biofilm in BHI medium. On the other hand, except for CIDCA 8321 strain that did not develop biofilm on any probed condition, the lactobacilli can form biofilm in MRS medium. The lowest biofilm production was observed in plastic, whereas in glass and stainless steel lactobacilli present high capacity of biofilm formation. It is remarkable that Lb. plantarum CIDCA 83114 is the one with the highest capacity for biofilm formation in the three surfaces studied, being stainless steel the most suitable surface for biofilm production. We observed that both hydrophobic and hydrophilic strains are capable of producing biofilm. We demonstrated that lactobacilli can form biofilm and this property depends on surface and growth media. This could be used as an alternative control of food pathogens.
Fil: Merino, Lina Ethel. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Fil: Trejo, Fernando Miguel. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Fil: de Antoni, Graciela Liliana. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
Fil: Golowczyc, Marina Alejandra. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Criotecnología de Alimentos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Criotecnología de Alimentos; Argentina
XII Congreso Argentino de Microbiología General
San Miguel de Tucuman
Argentina
Asociación Civil de Microbiología General
description Biofilms are complex structures of microorganisms that colonize various biotic or abiotic surfaces. These organized communities are formed by bacteria embedded in a highly hydrated extracellular matrix that is composed mainly of polysaccharides, proteins, DNA and other substances. Several bacterial surface structures are necessary for this surface adhesion and an environment that favors the formation of biofilm against planktonic growth is required. The presence of biofilms is common in food industry and represents a concern because bacteria can adhere to almost any type of surface,such as plastic, metal, glass, soil particles and wood. Lactic bacteria are generally recognized as safe (GRAS) and could be an alternative for the biocontrol of pathogenic microorganisms biofilms forming in the food production chain. Use of probiotic biofilms can be an alternative approach for reducing the formation of pathogenic biofilms in food industries.The objective of this work was to study the biofilm production of lactic acid bacteria in different substrates and in different surfaces such as plastic, glass and stainless steel, commonly used in food industry. Biofilm formation was measured by violet crystal and plaque counts of seven strains of lactobacilli: Lb. kefiri CIDCA 83113, CIDCA 8321, CIDCA 8344 and CIDCA 5818, Lb. plantarum CIDCA 83114 and CIDCA 8327 and Lb. delbrueckii subsp. lactis CIDCA 133. These have different characteristics such as presence of S-layer, exopolysaccharide or glucan production, autoaggregation and hydrophobicity.The percentage of autoaggregation by decreasing OD at 600 nm and the percentage of hydrophobicity by partition in hexadecane and xylene (MATH method) was measured. For measurement of biofilmformation, 1 ml of medium (MRS and BHI) was inoculated with lactobacilli in 24 wells culture plates.For viable counts, the adhered bacteria were resuspended in PBS and counted in MRS agar plates.Only Lb. kefiri CIDCA 8321 and CIDCA 8344 strains presented a percentage of autoaggregationgreater than 50%. Both strains also showed the highest hydrophobicity. None of the lactobacilli tested form biofilm in BHI medium. On the other hand, except for CIDCA 8321 strain that did not develop biofilm on any probed condition, the lactobacilli can form biofilm in MRS medium. The lowest biofilm production was observed in plastic, whereas in glass and stainless steel lactobacilli present high capacity of biofilm formation. It is remarkable that Lb. plantarum CIDCA 83114 is the one with the highest capacity for biofilm formation in the three surfaces studied, being stainless steel the most suitable surface for biofilm production. We observed that both hydrophobic and hydrophilic strains are capable of producing biofilm. We demonstrated that lactobacilli can form biofilm and this property depends on surface and growth media. This could be used as an alternative control of food pathogens.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/168455
Biofilm producing lactic acid bacterial as an alternative to control food contaminations; XII Congreso Argentino de Microbiología General; San Miguel de Tucuman; Argentina; 2017; 1-3
CONICET Digital
CONICET
url http://hdl.handle.net/11336/168455
identifier_str_mv Biofilm producing lactic acid bacterial as an alternative to control food contaminations; XII Congreso Argentino de Microbiología General; San Miguel de Tucuman; Argentina; 2017; 1-3
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.samige.org.ar/admin/news/files/108-Libro%20SAMIGE%202017.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv text/plain
text/richtext
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Asociación Civil de Microbiología General
publisher.none.fl_str_mv Asociación Civil de Microbiología General
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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