Bacterial biofilm evidence in nasal polyposis

Autores
Zernotti, Mario Emilio; Angel Villegas, Natalia; Roques Revol, M.; Baena Cagnani, C. E.; Arce Miranda, Julio Eduardo; Paredes, M. E.; Albesa, Inés; Paraje, María Gabriela
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The pathogeny of chronic rhinosinusitis with nasal polyposis (CRS/NP) has not been elucidated. Bacterial exotoxins have beenimplicated in many infl ammatory chronic diseases, such as chronic otitis, chronic tonsillitis, cholesteatomas, and more recently CRS/NP. Wepropose that the bacteria in CRS/NP are not only present in a planktonic state, but also occur in microbial communities as biofi lms.Objective: To determine and characterize the presence of biofi lms in CRS/NP.Methods: We performed a prospective study in 12 patients undergoing endoscopic sinus surgery for nasal polyposis. Ten patients withoutCRS/NP who underwent septoplasty were included as a control group. Tissue samples were obtained from the inferior turbinate mucosae.The bacteria were isolated and typifi ed and the material was examined in vitro using a spectrophotometer, and in vivo using opticalmicroscopy and confocal scanning laser microscopy.Results: Moderate to high in vitro biofi lm-forming capacity was detected in 9 out of 12 patients with CRS/NP (mean [SD] optical densityvalues of between 0.284 [0.017] and 3.337 [0.029]). The microorganisms isolated were Staphylococcus (5 patients), Streptococcus viridans,Pseudomonas aeruginosa, Enterococcus faecalis and Streptococcus viridans/Corynebacterium. Biofi lms were demonstrated in vivo in 2 patientsand no biofi lm structures were evident in any of the controls.Conclusion: This study demonstrates the presence of bacterial biofi lms in patients with CRS/NP. This chronic infl ammatory factor mightcontribute to nasal mucosa damage, increased infl ammatory cells in tissue, and the subsequent hyperplasic process
Fil: Zernotti, Mario Emilio. Sanatorio Allende. Servicio de Otorrinolaringología; Argentina
Fil: Angel Villegas, Natalia. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; Argentina
Fil: Roques Revol, M.. Sanatorio Allende. Servicio de Otorrinolaringología; Argentina
Fil: Baena Cagnani, C. E.. Universidad Católica de Córdoba; Argentina
Fil: Arce Miranda, Julio Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; Argentina
Fil: Paredes, M. E.. Sanatorio Allende. Departamento de Bacteriología; Argentina
Fil: Albesa, Inés. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; Argentina
Fil: Paraje, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; Argentina
Materia
Biofilms
Nasal Polylosis
Chronic Rhinosinusitis
Confocal Scanning Laser Microscopy
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/27305
Acceder
id CONICETDig_3c2edafc54d7aa89a84f2172c49fc6cc
oai_identifier_str oai:ri.conicet.gov.ar:11336/27305
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Bacterial biofilm evidence in nasal polyposisZernotti, Mario EmilioAngel Villegas, NataliaRoques Revol, M.Baena Cagnani, C. E.Arce Miranda, Julio EduardoParedes, M. E.Albesa, InésParaje, María GabrielaBiofilmsNasal PolylosisChronic RhinosinusitisConfocal Scanning Laser Microscopyhttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3The pathogeny of chronic rhinosinusitis with nasal polyposis (CRS/NP) has not been elucidated. Bacterial exotoxins have beenimplicated in many infl ammatory chronic diseases, such as chronic otitis, chronic tonsillitis, cholesteatomas, and more recently CRS/NP. Wepropose that the bacteria in CRS/NP are not only present in a planktonic state, but also occur in microbial communities as biofi lms.Objective: To determine and characterize the presence of biofi lms in CRS/NP.Methods: We performed a prospective study in 12 patients undergoing endoscopic sinus surgery for nasal polyposis. Ten patients withoutCRS/NP who underwent septoplasty were included as a control group. Tissue samples were obtained from the inferior turbinate mucosae.The bacteria were isolated and typifi ed and the material was examined in vitro using a spectrophotometer, and in vivo using opticalmicroscopy and confocal scanning laser microscopy.Results: Moderate to high in vitro biofi lm-forming capacity was detected in 9 out of 12 patients with CRS/NP (mean [SD] optical densityvalues of between 0.284 [0.017] and 3.337 [0.029]). The microorganisms isolated were Staphylococcus (5 patients), Streptococcus viridans,Pseudomonas aeruginosa, Enterococcus faecalis and Streptococcus viridans/Corynebacterium. Biofi lms were demonstrated in vivo in 2 patientsand no biofi lm structures were evident in any of the controls.Conclusion: This study demonstrates the presence of bacterial biofi lms in patients with CRS/NP. This chronic infl ammatory factor mightcontribute to nasal mucosa damage, increased infl ammatory cells in tissue, and the subsequent hyperplasic processFil: Zernotti, Mario Emilio. Sanatorio Allende. Servicio de Otorrinolaringología; ArgentinaFil: Angel Villegas, Natalia. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; ArgentinaFil: Roques Revol, M.. Sanatorio Allende. Servicio de Otorrinolaringología; ArgentinaFil: Baena Cagnani, C. E.. Universidad Católica de Córdoba; ArgentinaFil: Arce Miranda, Julio Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; ArgentinaFil: Paredes, M. E.. Sanatorio Allende. Departamento de Bacteriología; ArgentinaFil: Albesa, Inés. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; ArgentinaFil: Paraje, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; ArgentinaSociedad Española de Alergología e Inmunología Clínica2009-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/27305Zernotti, Mario Emilio; Angel Villegas, Natalia; Roques Revol, M.; Baena Cagnani, C. E.; Arce Miranda, Julio Eduardo; et al.; Bacterial biofilm evidence in nasal polyposis; Sociedad Española de Alergología e Inmunología Clínica; Journal Of Investigational Allergology And Clinical Immunology; 20; 5; 12-2009; 380-3851018-90681698-0808CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.jiaci.org/summary/vol20-issue5-num623info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:24:41Zoai:ri.conicet.gov.ar:11336/27305instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:24:41.358CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Bacterial biofilm evidence in nasal polyposis
title Bacterial biofilm evidence in nasal polyposis
spellingShingle Bacterial biofilm evidence in nasal polyposis
Zernotti, Mario Emilio
Biofilms
Nasal Polylosis
Chronic Rhinosinusitis
Confocal Scanning Laser Microscopy
title_short Bacterial biofilm evidence in nasal polyposis
title_full Bacterial biofilm evidence in nasal polyposis
title_fullStr Bacterial biofilm evidence in nasal polyposis
title_full_unstemmed Bacterial biofilm evidence in nasal polyposis
title_sort Bacterial biofilm evidence in nasal polyposis
dc.creator.none.fl_str_mv Zernotti, Mario Emilio
Angel Villegas, Natalia
Roques Revol, M.
Baena Cagnani, C. E.
Arce Miranda, Julio Eduardo
Paredes, M. E.
Albesa, Inés
Paraje, María Gabriela
author Zernotti, Mario Emilio
author_facet Zernotti, Mario Emilio
Angel Villegas, Natalia
Roques Revol, M.
Baena Cagnani, C. E.
Arce Miranda, Julio Eduardo
Paredes, M. E.
Albesa, Inés
Paraje, María Gabriela
author_role author
author2 Angel Villegas, Natalia
Roques Revol, M.
Baena Cagnani, C. E.
Arce Miranda, Julio Eduardo
Paredes, M. E.
Albesa, Inés
Paraje, María Gabriela
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Biofilms
Nasal Polylosis
Chronic Rhinosinusitis
Confocal Scanning Laser Microscopy
topic Biofilms
Nasal Polylosis
Chronic Rhinosinusitis
Confocal Scanning Laser Microscopy
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The pathogeny of chronic rhinosinusitis with nasal polyposis (CRS/NP) has not been elucidated. Bacterial exotoxins have beenimplicated in many infl ammatory chronic diseases, such as chronic otitis, chronic tonsillitis, cholesteatomas, and more recently CRS/NP. Wepropose that the bacteria in CRS/NP are not only present in a planktonic state, but also occur in microbial communities as biofi lms.Objective: To determine and characterize the presence of biofi lms in CRS/NP.Methods: We performed a prospective study in 12 patients undergoing endoscopic sinus surgery for nasal polyposis. Ten patients withoutCRS/NP who underwent septoplasty were included as a control group. Tissue samples were obtained from the inferior turbinate mucosae.The bacteria were isolated and typifi ed and the material was examined in vitro using a spectrophotometer, and in vivo using opticalmicroscopy and confocal scanning laser microscopy.Results: Moderate to high in vitro biofi lm-forming capacity was detected in 9 out of 12 patients with CRS/NP (mean [SD] optical densityvalues of between 0.284 [0.017] and 3.337 [0.029]). The microorganisms isolated were Staphylococcus (5 patients), Streptococcus viridans,Pseudomonas aeruginosa, Enterococcus faecalis and Streptococcus viridans/Corynebacterium. Biofi lms were demonstrated in vivo in 2 patientsand no biofi lm structures were evident in any of the controls.Conclusion: This study demonstrates the presence of bacterial biofi lms in patients with CRS/NP. This chronic infl ammatory factor mightcontribute to nasal mucosa damage, increased infl ammatory cells in tissue, and the subsequent hyperplasic process
Fil: Zernotti, Mario Emilio. Sanatorio Allende. Servicio de Otorrinolaringología; Argentina
Fil: Angel Villegas, Natalia. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; Argentina
Fil: Roques Revol, M.. Sanatorio Allende. Servicio de Otorrinolaringología; Argentina
Fil: Baena Cagnani, C. E.. Universidad Católica de Córdoba; Argentina
Fil: Arce Miranda, Julio Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; Argentina
Fil: Paredes, M. E.. Sanatorio Allende. Departamento de Bacteriología; Argentina
Fil: Albesa, Inés. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; Argentina
Fil: Paraje, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Farmacia; Argentina
description The pathogeny of chronic rhinosinusitis with nasal polyposis (CRS/NP) has not been elucidated. Bacterial exotoxins have beenimplicated in many infl ammatory chronic diseases, such as chronic otitis, chronic tonsillitis, cholesteatomas, and more recently CRS/NP. Wepropose that the bacteria in CRS/NP are not only present in a planktonic state, but also occur in microbial communities as biofi lms.Objective: To determine and characterize the presence of biofi lms in CRS/NP.Methods: We performed a prospective study in 12 patients undergoing endoscopic sinus surgery for nasal polyposis. Ten patients withoutCRS/NP who underwent septoplasty were included as a control group. Tissue samples were obtained from the inferior turbinate mucosae.The bacteria were isolated and typifi ed and the material was examined in vitro using a spectrophotometer, and in vivo using opticalmicroscopy and confocal scanning laser microscopy.Results: Moderate to high in vitro biofi lm-forming capacity was detected in 9 out of 12 patients with CRS/NP (mean [SD] optical densityvalues of between 0.284 [0.017] and 3.337 [0.029]). The microorganisms isolated were Staphylococcus (5 patients), Streptococcus viridans,Pseudomonas aeruginosa, Enterococcus faecalis and Streptococcus viridans/Corynebacterium. Biofi lms were demonstrated in vivo in 2 patientsand no biofi lm structures were evident in any of the controls.Conclusion: This study demonstrates the presence of bacterial biofi lms in patients with CRS/NP. This chronic infl ammatory factor mightcontribute to nasal mucosa damage, increased infl ammatory cells in tissue, and the subsequent hyperplasic process
publishDate 2009
dc.date.none.fl_str_mv 2009-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/27305
Zernotti, Mario Emilio; Angel Villegas, Natalia; Roques Revol, M.; Baena Cagnani, C. E.; Arce Miranda, Julio Eduardo; et al.; Bacterial biofilm evidence in nasal polyposis; Sociedad Española de Alergología e Inmunología Clínica; Journal Of Investigational Allergology And Clinical Immunology; 20; 5; 12-2009; 380-385
1018-9068
1698-0808
CONICET Digital
CONICET
url http://hdl.handle.net/11336/27305
identifier_str_mv Zernotti, Mario Emilio; Angel Villegas, Natalia; Roques Revol, M.; Baena Cagnani, C. E.; Arce Miranda, Julio Eduardo; et al.; Bacterial biofilm evidence in nasal polyposis; Sociedad Española de Alergología e Inmunología Clínica; Journal Of Investigational Allergology And Clinical Immunology; 20; 5; 12-2009; 380-385
1018-9068
1698-0808
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.jiaci.org/summary/vol20-issue5-num623
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Sociedad Española de Alergología e Inmunología Clínica
publisher.none.fl_str_mv Sociedad Española de Alergología e Inmunología Clínica
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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