Allergenicity reduction of cow's milk proteins using latex peptidases

Autores
Oliveira, João P. B.; Candreva, Ángela María; Rizzo, Gaston Pascual; Ramos, Márcio V.; Oliveira, Jefferson S.; Oliveira, Hermógenes D.; Ary, Maria B.; Docena, Guillermo H.; Freitas, Cleverson D. T.
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The present study evaluated four laticifer fluids as a novel source of peptidases capable of hydrolyzing proteins in cow´s milk. The latex peptidases from Calotropis procera (CpLP), Cryptostegia grandiflora (CgLP), and Carica papaya (CapLP) were able to perform total hydrolysis of caseins after 30 min at pH 6.5, as confirmed by a significant reduction in the residual antigenicity. Casein hydrolysis by Plumeria rubra latex peptidases (PrLP) was negligible. Moreover, whey proteins were more resistant to proteolysis by latex peptidases; however, heat pretreatment of the whey proteins enhanced the degree of hydrolysis and reduced the residual antigenicity of the hydrolysates. The in vivo assays show that the cow´s milk proteins hydrolysed by CgLP and CapLP exhibited no immune reactions in mice allergic to cow´s milk, similar to a commercial partially hydrolysed formula. Thus, these peptidases are promising enzymes for the development of novel hypoallergenic formulas for children with a milk allergy.
Fil: Oliveira, João P. B.. Universidade Estadual do Ceará; Brasil
Fil: Candreva, Ángela María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Estudios Inmunológicos y Fisiopatológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Estudios Inmunológicos y Fisiopatológicos; Argentina
Fil: Rizzo, Gaston Pascual. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Estudios Inmunológicos y Fisiopatológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Estudios Inmunológicos y Fisiopatológicos; Argentina
Fil: Ramos, Márcio V.. Universidade Estadual do Ceará; Brasil
Fil: Oliveira, Jefferson S.. Universidade Estadual do Ceará; Brasil
Fil: Oliveira, Hermógenes D.. Universidade Federal Do Piaui.; Brasil
Fil: Ary, Maria B.. Universidade Estadual do Ceará; Brasil
Fil: Docena, Guillermo H.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Estudios Inmunológicos y Fisiopatológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Estudios Inmunológicos y Fisiopatológicos; Argentina
Fil: Freitas, Cleverson D. T.. Universidade Federal Do Ceara; Brasil
Materia
Milk Allergy
latex peptidases
novel hypoallergenic formulas
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/131672
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/131672
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Allergenicity reduction of cow's milk proteins using latex peptidasesOliveira, João P. B.Candreva, Ángela MaríaRizzo, Gaston PascualRamos, Márcio V.Oliveira, Jefferson S.Oliveira, Hermógenes D.Ary, Maria B.Docena, Guillermo H.Freitas, Cleverson D. T.Milk Allergylatex peptidasesnovel hypoallergenic formulashttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3The present study evaluated four laticifer fluids as a novel source of peptidases capable of hydrolyzing proteins in cow´s milk. The latex peptidases from Calotropis procera (CpLP), Cryptostegia grandiflora (CgLP), and Carica papaya (CapLP) were able to perform total hydrolysis of caseins after 30 min at pH 6.5, as confirmed by a significant reduction in the residual antigenicity. Casein hydrolysis by Plumeria rubra latex peptidases (PrLP) was negligible. Moreover, whey proteins were more resistant to proteolysis by latex peptidases; however, heat pretreatment of the whey proteins enhanced the degree of hydrolysis and reduced the residual antigenicity of the hydrolysates. The in vivo assays show that the cow´s milk proteins hydrolysed by CgLP and CapLP exhibited no immune reactions in mice allergic to cow´s milk, similar to a commercial partially hydrolysed formula. Thus, these peptidases are promising enzymes for the development of novel hypoallergenic formulas for children with a milk allergy.Fil: Oliveira, João P. B.. Universidade Estadual do Ceará; BrasilFil: Candreva, Ángela María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Estudios Inmunológicos y Fisiopatológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Estudios Inmunológicos y Fisiopatológicos; ArgentinaFil: Rizzo, Gaston Pascual. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Estudios Inmunológicos y Fisiopatológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Estudios Inmunológicos y Fisiopatológicos; ArgentinaFil: Ramos, Márcio V.. Universidade Estadual do Ceará; BrasilFil: Oliveira, Jefferson S.. Universidade Estadual do Ceará; BrasilFil: Oliveira, Hermógenes D.. Universidade Federal Do Piaui.; BrasilFil: Ary, Maria B.. Universidade Estadual do Ceará; BrasilFil: Docena, Guillermo H.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Estudios Inmunológicos y Fisiopatológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Estudios Inmunológicos y Fisiopatológicos; ArgentinaFil: Freitas, Cleverson D. T.. Universidade Federal Do Ceara; BrasilElsevier2019-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/131672Oliveira, João P. B.; Candreva, Ángela María; Rizzo, Gaston Pascual; Ramos, Márcio V.; Oliveira, Jefferson S.; et al.; Allergenicity reduction of cow's milk proteins using latex peptidases; Elsevier; Food Chemistry; 284; 6-2019; 245-2530308-8146CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0308814619301980info:eu-repo/semantics/altIdentifier/doi/10.1016/j.foodchem.2019.01.123info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:52:13Zoai:ri.conicet.gov.ar:11336/131672instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:52:14.104CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Allergenicity reduction of cow's milk proteins using latex peptidases
title Allergenicity reduction of cow's milk proteins using latex peptidases
spellingShingle Allergenicity reduction of cow's milk proteins using latex peptidases
Oliveira, João P. B.
Milk Allergy
latex peptidases
novel hypoallergenic formulas
title_short Allergenicity reduction of cow's milk proteins using latex peptidases
title_full Allergenicity reduction of cow's milk proteins using latex peptidases
title_fullStr Allergenicity reduction of cow's milk proteins using latex peptidases
title_full_unstemmed Allergenicity reduction of cow's milk proteins using latex peptidases
title_sort Allergenicity reduction of cow's milk proteins using latex peptidases
dc.creator.none.fl_str_mv Oliveira, João P. B.
Candreva, Ángela María
Rizzo, Gaston Pascual
Ramos, Márcio V.
Oliveira, Jefferson S.
Oliveira, Hermógenes D.
Ary, Maria B.
Docena, Guillermo H.
Freitas, Cleverson D. T.
author Oliveira, João P. B.
author_facet Oliveira, João P. B.
Candreva, Ángela María
Rizzo, Gaston Pascual
Ramos, Márcio V.
Oliveira, Jefferson S.
Oliveira, Hermógenes D.
Ary, Maria B.
Docena, Guillermo H.
Freitas, Cleverson D. T.
author_role author
author2 Candreva, Ángela María
Rizzo, Gaston Pascual
Ramos, Márcio V.
Oliveira, Jefferson S.
Oliveira, Hermógenes D.
Ary, Maria B.
Docena, Guillermo H.
Freitas, Cleverson D. T.
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Milk Allergy
latex peptidases
novel hypoallergenic formulas
topic Milk Allergy
latex peptidases
novel hypoallergenic formulas
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The present study evaluated four laticifer fluids as a novel source of peptidases capable of hydrolyzing proteins in cow´s milk. The latex peptidases from Calotropis procera (CpLP), Cryptostegia grandiflora (CgLP), and Carica papaya (CapLP) were able to perform total hydrolysis of caseins after 30 min at pH 6.5, as confirmed by a significant reduction in the residual antigenicity. Casein hydrolysis by Plumeria rubra latex peptidases (PrLP) was negligible. Moreover, whey proteins were more resistant to proteolysis by latex peptidases; however, heat pretreatment of the whey proteins enhanced the degree of hydrolysis and reduced the residual antigenicity of the hydrolysates. The in vivo assays show that the cow´s milk proteins hydrolysed by CgLP and CapLP exhibited no immune reactions in mice allergic to cow´s milk, similar to a commercial partially hydrolysed formula. Thus, these peptidases are promising enzymes for the development of novel hypoallergenic formulas for children with a milk allergy.
Fil: Oliveira, João P. B.. Universidade Estadual do Ceará; Brasil
Fil: Candreva, Ángela María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Estudios Inmunológicos y Fisiopatológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Estudios Inmunológicos y Fisiopatológicos; Argentina
Fil: Rizzo, Gaston Pascual. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Estudios Inmunológicos y Fisiopatológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Estudios Inmunológicos y Fisiopatológicos; Argentina
Fil: Ramos, Márcio V.. Universidade Estadual do Ceará; Brasil
Fil: Oliveira, Jefferson S.. Universidade Estadual do Ceará; Brasil
Fil: Oliveira, Hermógenes D.. Universidade Federal Do Piaui.; Brasil
Fil: Ary, Maria B.. Universidade Estadual do Ceará; Brasil
Fil: Docena, Guillermo H.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Estudios Inmunológicos y Fisiopatológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Estudios Inmunológicos y Fisiopatológicos; Argentina
Fil: Freitas, Cleverson D. T.. Universidade Federal Do Ceara; Brasil
description The present study evaluated four laticifer fluids as a novel source of peptidases capable of hydrolyzing proteins in cow´s milk. The latex peptidases from Calotropis procera (CpLP), Cryptostegia grandiflora (CgLP), and Carica papaya (CapLP) were able to perform total hydrolysis of caseins after 30 min at pH 6.5, as confirmed by a significant reduction in the residual antigenicity. Casein hydrolysis by Plumeria rubra latex peptidases (PrLP) was negligible. Moreover, whey proteins were more resistant to proteolysis by latex peptidases; however, heat pretreatment of the whey proteins enhanced the degree of hydrolysis and reduced the residual antigenicity of the hydrolysates. The in vivo assays show that the cow´s milk proteins hydrolysed by CgLP and CapLP exhibited no immune reactions in mice allergic to cow´s milk, similar to a commercial partially hydrolysed formula. Thus, these peptidases are promising enzymes for the development of novel hypoallergenic formulas for children with a milk allergy.
publishDate 2019
dc.date.none.fl_str_mv 2019-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/131672
Oliveira, João P. B.; Candreva, Ángela María; Rizzo, Gaston Pascual; Ramos, Márcio V.; Oliveira, Jefferson S.; et al.; Allergenicity reduction of cow's milk proteins using latex peptidases; Elsevier; Food Chemistry; 284; 6-2019; 245-253
0308-8146
CONICET Digital
CONICET
url http://hdl.handle.net/11336/131672
identifier_str_mv Oliveira, João P. B.; Candreva, Ángela María; Rizzo, Gaston Pascual; Ramos, Márcio V.; Oliveira, Jefferson S.; et al.; Allergenicity reduction of cow's milk proteins using latex peptidases; Elsevier; Food Chemistry; 284; 6-2019; 245-253
0308-8146
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0308814619301980
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.foodchem.2019.01.123
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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