Comparative transcriptomic, proteomic, and amino acid content analyses of quinoa (Chenopodium quinoa) seed accessions from diverse geographic locations

Autores
Barberini, María Laura; Lee, Yun Sun; Guerrero, Leandro Demián; Obertello, Mariana; Mazzella, Maria Agustina; Bertero, Hector Daniel; Grotewold, Erich; Muschietti, Jorge Prometeo
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We analyzed the quinoa seed transcriptome of four different accessions by aligning the RNA-seq results with the second version of the QQ74 genome (QQ74-V2). After filtering and normalizing 29,355 transcripts, we observed that QQ74 was most distinct compared to NWA accessions. Subsequently, we performed a transcript differential analysis in the ten pairwise comparisons and identified a total of 3,014 differentially expressed genes (DEGs). A Gene Ontology (GO) enrichment analysis of these DEGs showed that the accession representing the NWA transition area was enriched in terms related to oxidative phosphorylation and heat response. Furthermore, using 2D-gel electrophoresis followed by mass spectrometry, we demonstrated that storage proteins, specifically 11S globulin, 13S globulin and vicilin-like antimicrobial peptides, were the most abundant proteins in quinoa seeds of the five accessions. In addition, we observed that some of these proteins were differentially expressed between accessions. In terms of total amino acid content, statistically significant differences between some of the accessions were found only for lysine and leucine.
Fil: Barberini, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Lee, Yun Sun. Michigan State University; Estados Unidos. Seoul National University; Corea del Sur
Fil: Guerrero, Leandro Demián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Obertello, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Mazzella, Maria Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Bertero, Hector Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina
Fil: Grotewold, Erich. Michigan State University; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Muschietti, Jorge Prometeo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Materia
quinoa
semillas
transcriptoma
proteoma
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/280910
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/280910
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Comparative transcriptomic, proteomic, and amino acid content analyses of quinoa (Chenopodium quinoa) seed accessions from diverse geographic locationsBarberini, María LauraLee, Yun SunGuerrero, Leandro DemiánObertello, MarianaMazzella, Maria AgustinaBertero, Hector DanielGrotewold, ErichMuschietti, Jorge Prometeoquinoasemillastranscriptomaproteomahttps://purl.org/becyt/ford/4.5https://purl.org/becyt/ford/4We analyzed the quinoa seed transcriptome of four different accessions by aligning the RNA-seq results with the second version of the QQ74 genome (QQ74-V2). After filtering and normalizing 29,355 transcripts, we observed that QQ74 was most distinct compared to NWA accessions. Subsequently, we performed a transcript differential analysis in the ten pairwise comparisons and identified a total of 3,014 differentially expressed genes (DEGs). A Gene Ontology (GO) enrichment analysis of these DEGs showed that the accession representing the NWA transition area was enriched in terms related to oxidative phosphorylation and heat response. Furthermore, using 2D-gel electrophoresis followed by mass spectrometry, we demonstrated that storage proteins, specifically 11S globulin, 13S globulin and vicilin-like antimicrobial peptides, were the most abundant proteins in quinoa seeds of the five accessions. In addition, we observed that some of these proteins were differentially expressed between accessions. In terms of total amino acid content, statistically significant differences between some of the accessions were found only for lysine and leucine.Fil: Barberini, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Lee, Yun Sun. Michigan State University; Estados Unidos. Seoul National University; Corea del SurFil: Guerrero, Leandro Demián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Obertello, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Mazzella, Maria Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Bertero, Hector Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; ArgentinaFil: Grotewold, Erich. Michigan State University; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Muschietti, Jorge Prometeo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaBioMed Central2025-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/280910Barberini, María Laura; Lee, Yun Sun; Guerrero, Leandro Demián; Obertello, Mariana; Mazzella, Maria Agustina; et al.; Comparative transcriptomic, proteomic, and amino acid content analyses of quinoa (Chenopodium quinoa) seed accessions from diverse geographic locations; BioMed Central; BMC Plant Biology; 25; 1; 7-2025; 1-151471-2229CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-025-06913-zinfo:eu-repo/semantics/altIdentifier/doi/10.1186/s12870-025-06913-zinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-02-11T12:01:19Zoai:ri.conicet.gov.ar:11336/280910instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-02-11 12:01:19.701CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Comparative transcriptomic, proteomic, and amino acid content analyses of quinoa (Chenopodium quinoa) seed accessions from diverse geographic locations
title Comparative transcriptomic, proteomic, and amino acid content analyses of quinoa (Chenopodium quinoa) seed accessions from diverse geographic locations
spellingShingle Comparative transcriptomic, proteomic, and amino acid content analyses of quinoa (Chenopodium quinoa) seed accessions from diverse geographic locations
Barberini, María Laura
quinoa
semillas
transcriptoma
proteoma
title_short Comparative transcriptomic, proteomic, and amino acid content analyses of quinoa (Chenopodium quinoa) seed accessions from diverse geographic locations
title_full Comparative transcriptomic, proteomic, and amino acid content analyses of quinoa (Chenopodium quinoa) seed accessions from diverse geographic locations
title_fullStr Comparative transcriptomic, proteomic, and amino acid content analyses of quinoa (Chenopodium quinoa) seed accessions from diverse geographic locations
title_full_unstemmed Comparative transcriptomic, proteomic, and amino acid content analyses of quinoa (Chenopodium quinoa) seed accessions from diverse geographic locations
title_sort Comparative transcriptomic, proteomic, and amino acid content analyses of quinoa (Chenopodium quinoa) seed accessions from diverse geographic locations
dc.creator.none.fl_str_mv Barberini, María Laura
Lee, Yun Sun
Guerrero, Leandro Demián
Obertello, Mariana
Mazzella, Maria Agustina
Bertero, Hector Daniel
Grotewold, Erich
Muschietti, Jorge Prometeo
author Barberini, María Laura
author_facet Barberini, María Laura
Lee, Yun Sun
Guerrero, Leandro Demián
Obertello, Mariana
Mazzella, Maria Agustina
Bertero, Hector Daniel
Grotewold, Erich
Muschietti, Jorge Prometeo
author_role author
author2 Lee, Yun Sun
Guerrero, Leandro Demián
Obertello, Mariana
Mazzella, Maria Agustina
Bertero, Hector Daniel
Grotewold, Erich
Muschietti, Jorge Prometeo
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv quinoa
semillas
transcriptoma
proteoma
topic quinoa
semillas
transcriptoma
proteoma
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.5
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv We analyzed the quinoa seed transcriptome of four different accessions by aligning the RNA-seq results with the second version of the QQ74 genome (QQ74-V2). After filtering and normalizing 29,355 transcripts, we observed that QQ74 was most distinct compared to NWA accessions. Subsequently, we performed a transcript differential analysis in the ten pairwise comparisons and identified a total of 3,014 differentially expressed genes (DEGs). A Gene Ontology (GO) enrichment analysis of these DEGs showed that the accession representing the NWA transition area was enriched in terms related to oxidative phosphorylation and heat response. Furthermore, using 2D-gel electrophoresis followed by mass spectrometry, we demonstrated that storage proteins, specifically 11S globulin, 13S globulin and vicilin-like antimicrobial peptides, were the most abundant proteins in quinoa seeds of the five accessions. In addition, we observed that some of these proteins were differentially expressed between accessions. In terms of total amino acid content, statistically significant differences between some of the accessions were found only for lysine and leucine.
Fil: Barberini, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Lee, Yun Sun. Michigan State University; Estados Unidos. Seoul National University; Corea del Sur
Fil: Guerrero, Leandro Demián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Obertello, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Mazzella, Maria Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
Fil: Bertero, Hector Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina
Fil: Grotewold, Erich. Michigan State University; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Muschietti, Jorge Prometeo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
description We analyzed the quinoa seed transcriptome of four different accessions by aligning the RNA-seq results with the second version of the QQ74 genome (QQ74-V2). After filtering and normalizing 29,355 transcripts, we observed that QQ74 was most distinct compared to NWA accessions. Subsequently, we performed a transcript differential analysis in the ten pairwise comparisons and identified a total of 3,014 differentially expressed genes (DEGs). A Gene Ontology (GO) enrichment analysis of these DEGs showed that the accession representing the NWA transition area was enriched in terms related to oxidative phosphorylation and heat response. Furthermore, using 2D-gel electrophoresis followed by mass spectrometry, we demonstrated that storage proteins, specifically 11S globulin, 13S globulin and vicilin-like antimicrobial peptides, were the most abundant proteins in quinoa seeds of the five accessions. In addition, we observed that some of these proteins were differentially expressed between accessions. In terms of total amino acid content, statistically significant differences between some of the accessions were found only for lysine and leucine.
publishDate 2025
dc.date.none.fl_str_mv 2025-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/280910
Barberini, María Laura; Lee, Yun Sun; Guerrero, Leandro Demián; Obertello, Mariana; Mazzella, Maria Agustina; et al.; Comparative transcriptomic, proteomic, and amino acid content analyses of quinoa (Chenopodium quinoa) seed accessions from diverse geographic locations; BioMed Central; BMC Plant Biology; 25; 1; 7-2025; 1-15
1471-2229
CONICET Digital
CONICET
url http://hdl.handle.net/11336/280910
identifier_str_mv Barberini, María Laura; Lee, Yun Sun; Guerrero, Leandro Demián; Obertello, Mariana; Mazzella, Maria Agustina; et al.; Comparative transcriptomic, proteomic, and amino acid content analyses of quinoa (Chenopodium quinoa) seed accessions from diverse geographic locations; BioMed Central; BMC Plant Biology; 25; 1; 7-2025; 1-15
1471-2229
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-025-06913-z
info:eu-repo/semantics/altIdentifier/doi/10.1186/s12870-025-06913-z
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.930639

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