Study of Oxidative Stress and Enotoxicity by Arsenic Contamination in Glycine max. L

Autores
Marsa, Silvana Mariel; Rossi, Franco Rubén; Valdez, Hugo Alberto; Gomez Casati, Diego Fabian; Zirulnik, Fanny
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Introduction: Volcanism is an important natural origin of Arsenic (As) contamination. Aims: Oxidative stress parameters, antioxidant defense system, the expression of transcripts involved in defense responses and the As genotoxicity were analyzed. Study Design: The plants were separated into two groups (Control and As-treated ones) until they were exposed to As (2.5 and 5 ppm) at day 10. Methodology: Biochemical, molecular and cytogenetic determinations were carried out. Results: Arsenic treated plants show growth abnormalities. The antioxidant defenses and oxidative parameters in roots and leaves showed different tissue-specific responses. Whereas leaf tissues display an active antioxidant defense, root cells are unable to produce defense molecules andshow oxidative stress. Moreover, the analysis of antioxidant enzymes such as ascorbate peroxidase and superoxide dismutase, showed higher activity in leaves than in roots, except for isocitrate dehydrogenase. The expression of GR and MAPK decreased significantly in all the treatments during the time curve. ICDH showed an increase in its expression with 24 hours of treatment and falls at 72 hours compared to their controls. Genotoxicity assays showed a decrease of mitotic index (MI) and the presence of altered nucleus, in samples exposed for 72 hours. Conclusion: Our results suggest that the physiological, biochemical, molecular and cytogenetic behaviorbehavior of the plants depends on the As concentration and the length of the exposure.
Fil: Marsa, Silvana Mariel. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Rossi, Franco Rubén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "dr. Raul Alfonsin" (sede Chascomus) | Universidad Nacional de San Martin. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas ; Argentina
Fil: Valdez, Hugo Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "dr. Raul Alfonsin" (sede Chascomus) | Universidad Nacional de San Martin. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas ; Argentina
Fil: Gomez Casati, Diego Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosinteticos y Bioquimicos. Universidad Nacional de Rosario. Facultad de Cs.bioquímicas y Farmaceuticas. Centro de Estudios Fotosinteticos y Bioquimicos; Argentina
Fil: Zirulnik, Fanny. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Materia
Arsenic
Soybean
Oxidative Stress
Antioxidant Defense
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/16091
Acceder
id CONICETDig_c962e134f39c481f44282508073f31aa
oai_identifier_str oai:ri.conicet.gov.ar:11336/16091
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Study of Oxidative Stress and Enotoxicity by Arsenic Contamination in Glycine max. LMarsa, Silvana MarielRossi, Franco RubénValdez, Hugo AlbertoGomez Casati, Diego FabianZirulnik, FannyArsenicSoybeanOxidative StressAntioxidant Defensehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Introduction: Volcanism is an important natural origin of Arsenic (As) contamination. Aims: Oxidative stress parameters, antioxidant defense system, the expression of transcripts involved in defense responses and the As genotoxicity were analyzed. Study Design: The plants were separated into two groups (Control and As-treated ones) until they were exposed to As (2.5 and 5 ppm) at day 10. Methodology: Biochemical, molecular and cytogenetic determinations were carried out. Results: Arsenic treated plants show growth abnormalities. The antioxidant defenses and oxidative parameters in roots and leaves showed different tissue-specific responses. Whereas leaf tissues display an active antioxidant defense, root cells are unable to produce defense molecules andshow oxidative stress. Moreover, the analysis of antioxidant enzymes such as ascorbate peroxidase and superoxide dismutase, showed higher activity in leaves than in roots, except for isocitrate dehydrogenase. The expression of GR and MAPK decreased significantly in all the treatments during the time curve. ICDH showed an increase in its expression with 24 hours of treatment and falls at 72 hours compared to their controls. Genotoxicity assays showed a decrease of mitotic index (MI) and the presence of altered nucleus, in samples exposed for 72 hours. Conclusion: Our results suggest that the physiological, biochemical, molecular and cytogenetic behaviorbehavior of the plants depends on the As concentration and the length of the exposure.Fil: Marsa, Silvana Mariel. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; ArgentinaFil: Rossi, Franco Rubén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "dr. Raul Alfonsin" (sede Chascomus) | Universidad Nacional de San Martin. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas ; ArgentinaFil: Valdez, Hugo Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "dr. Raul Alfonsin" (sede Chascomus) | Universidad Nacional de San Martin. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas ; ArgentinaFil: Gomez Casati, Diego Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosinteticos y Bioquimicos. Universidad Nacional de Rosario. Facultad de Cs.bioquímicas y Farmaceuticas. Centro de Estudios Fotosinteticos y Bioquimicos; ArgentinaFil: Zirulnik, Fanny. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; ArgentinaSciencedomain International2015-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/16091Marsa, Silvana Mariel; Rossi, Franco Rubén; Valdez, Hugo Alberto; Gomez Casati, Diego Fabian; Zirulnik, Fanny; Study of Oxidative Stress and Enotoxicity by Arsenic Contamination in Glycine max. L; Sciencedomain International; International Journal of Plant & Soil Science; 8; 1; 6-2015; 1-152320-7035enginfo:eu-repo/semantics/altIdentifier/doi/10.9734/IJPSS/2015/18979info:eu-repo/semantics/altIdentifier/url/http://sciencedomain.org/abstract/10034info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:06:24Zoai:ri.conicet.gov.ar:11336/16091instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:06:24.602CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Study of Oxidative Stress and Enotoxicity by Arsenic Contamination in Glycine max. L
title Study of Oxidative Stress and Enotoxicity by Arsenic Contamination in Glycine max. L
spellingShingle Study of Oxidative Stress and Enotoxicity by Arsenic Contamination in Glycine max. L
Marsa, Silvana Mariel
Arsenic
Soybean
Oxidative Stress
Antioxidant Defense
title_short Study of Oxidative Stress and Enotoxicity by Arsenic Contamination in Glycine max. L
title_full Study of Oxidative Stress and Enotoxicity by Arsenic Contamination in Glycine max. L
title_fullStr Study of Oxidative Stress and Enotoxicity by Arsenic Contamination in Glycine max. L
title_full_unstemmed Study of Oxidative Stress and Enotoxicity by Arsenic Contamination in Glycine max. L
title_sort Study of Oxidative Stress and Enotoxicity by Arsenic Contamination in Glycine max. L
dc.creator.none.fl_str_mv Marsa, Silvana Mariel
Rossi, Franco Rubén
Valdez, Hugo Alberto
Gomez Casati, Diego Fabian
Zirulnik, Fanny
author Marsa, Silvana Mariel
author_facet Marsa, Silvana Mariel
Rossi, Franco Rubén
Valdez, Hugo Alberto
Gomez Casati, Diego Fabian
Zirulnik, Fanny
author_role author
author2 Rossi, Franco Rubén
Valdez, Hugo Alberto
Gomez Casati, Diego Fabian
Zirulnik, Fanny
author2_role author
author
author
author
dc.subject.none.fl_str_mv Arsenic
Soybean
Oxidative Stress
Antioxidant Defense
topic Arsenic
Soybean
Oxidative Stress
Antioxidant Defense
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Introduction: Volcanism is an important natural origin of Arsenic (As) contamination. Aims: Oxidative stress parameters, antioxidant defense system, the expression of transcripts involved in defense responses and the As genotoxicity were analyzed. Study Design: The plants were separated into two groups (Control and As-treated ones) until they were exposed to As (2.5 and 5 ppm) at day 10. Methodology: Biochemical, molecular and cytogenetic determinations were carried out. Results: Arsenic treated plants show growth abnormalities. The antioxidant defenses and oxidative parameters in roots and leaves showed different tissue-specific responses. Whereas leaf tissues display an active antioxidant defense, root cells are unable to produce defense molecules andshow oxidative stress. Moreover, the analysis of antioxidant enzymes such as ascorbate peroxidase and superoxide dismutase, showed higher activity in leaves than in roots, except for isocitrate dehydrogenase. The expression of GR and MAPK decreased significantly in all the treatments during the time curve. ICDH showed an increase in its expression with 24 hours of treatment and falls at 72 hours compared to their controls. Genotoxicity assays showed a decrease of mitotic index (MI) and the presence of altered nucleus, in samples exposed for 72 hours. Conclusion: Our results suggest that the physiological, biochemical, molecular and cytogenetic behaviorbehavior of the plants depends on the As concentration and the length of the exposure.
Fil: Marsa, Silvana Mariel. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Rossi, Franco Rubén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "dr. Raul Alfonsin" (sede Chascomus) | Universidad Nacional de San Martin. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas ; Argentina
Fil: Valdez, Hugo Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "dr. Raul Alfonsin" (sede Chascomus) | Universidad Nacional de San Martin. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas ; Argentina
Fil: Gomez Casati, Diego Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosinteticos y Bioquimicos. Universidad Nacional de Rosario. Facultad de Cs.bioquímicas y Farmaceuticas. Centro de Estudios Fotosinteticos y Bioquimicos; Argentina
Fil: Zirulnik, Fanny. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
description Introduction: Volcanism is an important natural origin of Arsenic (As) contamination. Aims: Oxidative stress parameters, antioxidant defense system, the expression of transcripts involved in defense responses and the As genotoxicity were analyzed. Study Design: The plants were separated into two groups (Control and As-treated ones) until they were exposed to As (2.5 and 5 ppm) at day 10. Methodology: Biochemical, molecular and cytogenetic determinations were carried out. Results: Arsenic treated plants show growth abnormalities. The antioxidant defenses and oxidative parameters in roots and leaves showed different tissue-specific responses. Whereas leaf tissues display an active antioxidant defense, root cells are unable to produce defense molecules andshow oxidative stress. Moreover, the analysis of antioxidant enzymes such as ascorbate peroxidase and superoxide dismutase, showed higher activity in leaves than in roots, except for isocitrate dehydrogenase. The expression of GR and MAPK decreased significantly in all the treatments during the time curve. ICDH showed an increase in its expression with 24 hours of treatment and falls at 72 hours compared to their controls. Genotoxicity assays showed a decrease of mitotic index (MI) and the presence of altered nucleus, in samples exposed for 72 hours. Conclusion: Our results suggest that the physiological, biochemical, molecular and cytogenetic behaviorbehavior of the plants depends on the As concentration and the length of the exposure.
publishDate 2015
dc.date.none.fl_str_mv 2015-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/16091
Marsa, Silvana Mariel; Rossi, Franco Rubén; Valdez, Hugo Alberto; Gomez Casati, Diego Fabian; Zirulnik, Fanny; Study of Oxidative Stress and Enotoxicity by Arsenic Contamination in Glycine max. L; Sciencedomain International; International Journal of Plant & Soil Science; 8; 1; 6-2015; 1-15
2320-7035
url http://hdl.handle.net/11336/16091
identifier_str_mv Marsa, Silvana Mariel; Rossi, Franco Rubén; Valdez, Hugo Alberto; Gomez Casati, Diego Fabian; Zirulnik, Fanny; Study of Oxidative Stress and Enotoxicity by Arsenic Contamination in Glycine max. L; Sciencedomain International; International Journal of Plant & Soil Science; 8; 1; 6-2015; 1-15
2320-7035
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.9734/IJPSS/2015/18979
info:eu-repo/semantics/altIdentifier/url/http://sciencedomain.org/abstract/10034
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Sciencedomain International
publisher.none.fl_str_mv Sciencedomain International
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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