Characterization of Listeria spp. isolated from ready-to-eat products in Argentina using SDS-PAGE and restriction endonuclease

Autores
Pellicer, Karina; Copes, Julio Alberto; Nosetto, Edgardo Omar; Echeverria, Maria Gabriela
Año de publicación
2004
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Listeria spp. are considered of interest in public health since their presence indicates the potencial existence of L. monocytogenes. Total cellular proteins and DNA from four strains of L. monocytogenes serotype 4, four strains of L. monocytogenes belonging to serotype 1, twelve strains of L. innocua, four strains of L. seeligeri and two strains of L. welshimeri isolated from ready-to-eat food were studied by SDS-PAGE and restriction endonuclease digestion. SDS-PAGE protein profiles obtained were species specific and could be evaluated by visual comparison. Enzyme for restriction endonuclease analysis was EcoRI, discriminating L. monocytogenes from other Listeria spp. These methodologies might be a helpful tool and a good alternative for epidemiological tracking of listeriosis in laboratories, where other methods are not available.
Fil: Pellicer, Karina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Copes, Julio Alberto. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Nosetto, Edgardo Omar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Echeverria, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Materia
CHARACTERIZATION
DNA
LISTERIA
PROTEINS
READY-TO-EAT FOODS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/84819
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Characterization of Listeria spp. isolated from ready-to-eat products in Argentina using SDS-PAGE and restriction endonucleasePellicer, KarinaCopes, Julio AlbertoNosetto, Edgardo OmarEcheverria, Maria GabrielaCHARACTERIZATIONDNALISTERIAPROTEINSREADY-TO-EAT FOODShttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Listeria spp. are considered of interest in public health since their presence indicates the potencial existence of L. monocytogenes. Total cellular proteins and DNA from four strains of L. monocytogenes serotype 4, four strains of L. monocytogenes belonging to serotype 1, twelve strains of L. innocua, four strains of L. seeligeri and two strains of L. welshimeri isolated from ready-to-eat food were studied by SDS-PAGE and restriction endonuclease digestion. SDS-PAGE protein profiles obtained were species specific and could be evaluated by visual comparison. Enzyme for restriction endonuclease analysis was EcoRI, discriminating L. monocytogenes from other Listeria spp. These methodologies might be a helpful tool and a good alternative for epidemiological tracking of listeriosis in laboratories, where other methods are not available.Fil: Pellicer, Karina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Copes, Julio Alberto. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Nosetto, Edgardo Omar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Echeverria, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaElsevier Science2004-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/84819Pellicer, Karina; Copes, Julio Alberto; Nosetto, Edgardo Omar; Echeverria, Maria Gabriela; Characterization of Listeria spp. isolated from ready-to-eat products in Argentina using SDS-PAGE and restriction endonuclease; Elsevier Science; Food Research International; 37; 10; 9-2004; 1013-10190963-9969CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.foodres.2004.06.011info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0963996904001498info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:02:09Zoai:ri.conicet.gov.ar:11336/84819instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:02:09.605CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Characterization of Listeria spp. isolated from ready-to-eat products in Argentina using SDS-PAGE and restriction endonuclease
title Characterization of Listeria spp. isolated from ready-to-eat products in Argentina using SDS-PAGE and restriction endonuclease
spellingShingle Characterization of Listeria spp. isolated from ready-to-eat products in Argentina using SDS-PAGE and restriction endonuclease
Pellicer, Karina
CHARACTERIZATION
DNA
LISTERIA
PROTEINS
READY-TO-EAT FOODS
title_short Characterization of Listeria spp. isolated from ready-to-eat products in Argentina using SDS-PAGE and restriction endonuclease
title_full Characterization of Listeria spp. isolated from ready-to-eat products in Argentina using SDS-PAGE and restriction endonuclease
title_fullStr Characterization of Listeria spp. isolated from ready-to-eat products in Argentina using SDS-PAGE and restriction endonuclease
title_full_unstemmed Characterization of Listeria spp. isolated from ready-to-eat products in Argentina using SDS-PAGE and restriction endonuclease
title_sort Characterization of Listeria spp. isolated from ready-to-eat products in Argentina using SDS-PAGE and restriction endonuclease
dc.creator.none.fl_str_mv Pellicer, Karina
Copes, Julio Alberto
Nosetto, Edgardo Omar
Echeverria, Maria Gabriela
author Pellicer, Karina
author_facet Pellicer, Karina
Copes, Julio Alberto
Nosetto, Edgardo Omar
Echeverria, Maria Gabriela
author_role author
author2 Copes, Julio Alberto
Nosetto, Edgardo Omar
Echeverria, Maria Gabriela
author2_role author
author
author
dc.subject.none.fl_str_mv CHARACTERIZATION
DNA
LISTERIA
PROTEINS
READY-TO-EAT FOODS
topic CHARACTERIZATION
DNA
LISTERIA
PROTEINS
READY-TO-EAT FOODS
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Listeria spp. are considered of interest in public health since their presence indicates the potencial existence of L. monocytogenes. Total cellular proteins and DNA from four strains of L. monocytogenes serotype 4, four strains of L. monocytogenes belonging to serotype 1, twelve strains of L. innocua, four strains of L. seeligeri and two strains of L. welshimeri isolated from ready-to-eat food were studied by SDS-PAGE and restriction endonuclease digestion. SDS-PAGE protein profiles obtained were species specific and could be evaluated by visual comparison. Enzyme for restriction endonuclease analysis was EcoRI, discriminating L. monocytogenes from other Listeria spp. These methodologies might be a helpful tool and a good alternative for epidemiological tracking of listeriosis in laboratories, where other methods are not available.
Fil: Pellicer, Karina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Copes, Julio Alberto. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Nosetto, Edgardo Omar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Echeverria, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
description Listeria spp. are considered of interest in public health since their presence indicates the potencial existence of L. monocytogenes. Total cellular proteins and DNA from four strains of L. monocytogenes serotype 4, four strains of L. monocytogenes belonging to serotype 1, twelve strains of L. innocua, four strains of L. seeligeri and two strains of L. welshimeri isolated from ready-to-eat food were studied by SDS-PAGE and restriction endonuclease digestion. SDS-PAGE protein profiles obtained were species specific and could be evaluated by visual comparison. Enzyme for restriction endonuclease analysis was EcoRI, discriminating L. monocytogenes from other Listeria spp. These methodologies might be a helpful tool and a good alternative for epidemiological tracking of listeriosis in laboratories, where other methods are not available.
publishDate 2004
dc.date.none.fl_str_mv 2004-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/84819
Pellicer, Karina; Copes, Julio Alberto; Nosetto, Edgardo Omar; Echeverria, Maria Gabriela; Characterization of Listeria spp. isolated from ready-to-eat products in Argentina using SDS-PAGE and restriction endonuclease; Elsevier Science; Food Research International; 37; 10; 9-2004; 1013-1019
0963-9969
CONICET Digital
CONICET
url http://hdl.handle.net/11336/84819
identifier_str_mv Pellicer, Karina; Copes, Julio Alberto; Nosetto, Edgardo Omar; Echeverria, Maria Gabriela; Characterization of Listeria spp. isolated from ready-to-eat products in Argentina using SDS-PAGE and restriction endonuclease; Elsevier Science; Food Research International; 37; 10; 9-2004; 1013-1019
0963-9969
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.foodres.2004.06.011
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0963996904001498
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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