Pro-inflammatory cytokines and gelatinases in climatic droplet keratopathy

Autores
Holopainen, Juha M.; Robciuc, Alexandra; Cafaro, Thamara Analia; Suarez, Maria Fernanda; Konttinen, Yrjö T.; Alkatan, Hind M.; Tabbara, Khalid F.; Tervahartiala, Taina; Sorsa, Timo; Urrets Zavalía, Julio Alberto; Serra, Horacio Marcelo
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
PURPOSE. Climatic droplet keratopathy (CDK) is a degenerative disease of the cornea with possible involvement from matrix metalloproteinases (MMPs). Therefore, the authors investigated histologic distribution, levels, and molecular forms of MMP- 2 and MMP-9, as well as tear fluid levels of MMPs and cytokines in CDK patients. They additionally examined UV-B-irradiation effect on production of gelatinases and cytokines by human corneal epithelial (HCE) cell culture model. METHODS. Tears were collected from 20 unrelated individuals (10 with CDK and 10 controls). CDK affected corneas were haematoxylin-eosin stained and the presence and distribution of MMP-2 and MMP-9 was examined using immunohistochemistry. Gelatinases and cytokine secretion was measured in tears and supernatants from UV-B-exposed HCEs by immunoblotting, gelatin zymography, and protein array, respectively. RESULTS. MMP-2 and MMP-9 values were significantly higher in tears collected from CDK patients than healthy controls and were accompanied by pro-inflammatory cytokine secretion. Immunohistochemistry showed that MMP-2 was expressed at the basement membrane zone in both control and affected corneas, but also marked the edges of the granular CDK deposits; MMP-9 expression was restrained to basal layers of the epithelium and was markedly induced in CDK corneas. In HCE cells, UV-B increased gelatinase secretion, with a striking effect on MMP-9, and was preceded by pro-inflammatory cytokine release. CONCLUSIONS. The authors demonstrate that the corneal epithelium could participate in CDK development as a source of cytokines and gelatinases. Additionally, in HCE cells, UV-B- modulated cytokine and subsequent MMP secretion. Local inhibition of cytokine secretion and gelatinases may prevent CDK progression. © 2012 The Association for Research in Vision and Ophthalmology, Inc.
Fil: Holopainen, Juha M.. University Of Helsinski; Finlandia
Fil: Robciuc, Alexandra. University Of Helsinski; Finlandia
Fil: Cafaro, Thamara Analia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Suarez, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Konttinen, Yrjö T.. University Of Helsinski; Finlandia
Fil: Alkatan, Hind M.. The Eye Center and The Eye Foundation for Research in Ophthalmology; Arabia Saudita
Fil: Tabbara, Khalid F.. The Eye Center and The Eye Foundation for Research in Ophthalmology; Arabia Saudita
Fil: Tervahartiala, Taina. University Of Helsinski; Finlandia
Fil: Sorsa, Timo. University Of Helsinski; Finlandia
Fil: Urrets Zavalía, Julio Alberto. Universidad Catolica de Córdoba. Facultad de Medicina. Clinica Universitaria Reina Fabiola. Dto de Oftalmologia; Argentina
Fil: Serra, Horacio Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Materia
CYTOKINES
GELATINASES
CDK
CORNEA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/55146
Acceder
id CONICETDig_a1217b63314b337f66f038e19bab39f9
oai_identifier_str oai:ri.conicet.gov.ar:11336/55146
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Pro-inflammatory cytokines and gelatinases in climatic droplet keratopathyHolopainen, Juha M.Robciuc, AlexandraCafaro, Thamara AnaliaSuarez, Maria FernandaKonttinen, Yrjö T.Alkatan, Hind M.Tabbara, Khalid F.Tervahartiala, TainaSorsa, TimoUrrets Zavalía, Julio AlbertoSerra, Horacio MarceloCYTOKINESGELATINASESCDKCORNEAhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3PURPOSE. Climatic droplet keratopathy (CDK) is a degenerative disease of the cornea with possible involvement from matrix metalloproteinases (MMPs). Therefore, the authors investigated histologic distribution, levels, and molecular forms of MMP- 2 and MMP-9, as well as tear fluid levels of MMPs and cytokines in CDK patients. They additionally examined UV-B-irradiation effect on production of gelatinases and cytokines by human corneal epithelial (HCE) cell culture model. METHODS. Tears were collected from 20 unrelated individuals (10 with CDK and 10 controls). CDK affected corneas were haematoxylin-eosin stained and the presence and distribution of MMP-2 and MMP-9 was examined using immunohistochemistry. Gelatinases and cytokine secretion was measured in tears and supernatants from UV-B-exposed HCEs by immunoblotting, gelatin zymography, and protein array, respectively. RESULTS. MMP-2 and MMP-9 values were significantly higher in tears collected from CDK patients than healthy controls and were accompanied by pro-inflammatory cytokine secretion. Immunohistochemistry showed that MMP-2 was expressed at the basement membrane zone in both control and affected corneas, but also marked the edges of the granular CDK deposits; MMP-9 expression was restrained to basal layers of the epithelium and was markedly induced in CDK corneas. In HCE cells, UV-B increased gelatinase secretion, with a striking effect on MMP-9, and was preceded by pro-inflammatory cytokine release. CONCLUSIONS. The authors demonstrate that the corneal epithelium could participate in CDK development as a source of cytokines and gelatinases. Additionally, in HCE cells, UV-B- modulated cytokine and subsequent MMP secretion. Local inhibition of cytokine secretion and gelatinases may prevent CDK progression. © 2012 The Association for Research in Vision and Ophthalmology, Inc.Fil: Holopainen, Juha M.. University Of Helsinski; FinlandiaFil: Robciuc, Alexandra. University Of Helsinski; FinlandiaFil: Cafaro, Thamara Analia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Suarez, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Konttinen, Yrjö T.. University Of Helsinski; FinlandiaFil: Alkatan, Hind M.. The Eye Center and The Eye Foundation for Research in Ophthalmology; Arabia SauditaFil: Tabbara, Khalid F.. The Eye Center and The Eye Foundation for Research in Ophthalmology; Arabia SauditaFil: Tervahartiala, Taina. University Of Helsinski; FinlandiaFil: Sorsa, Timo. University Of Helsinski; FinlandiaFil: Urrets Zavalía, Julio Alberto. Universidad Catolica de Córdoba. Facultad de Medicina. Clinica Universitaria Reina Fabiola. Dto de Oftalmologia; ArgentinaFil: Serra, Horacio Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaAssociation for Research in Vision and Ophthalmology2012-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/55146Holopainen, Juha M.; Robciuc, Alexandra; Cafaro, Thamara Analia; Suarez, Maria Fernanda; Konttinen, Yrjö T. ; et al.; Pro-inflammatory cytokines and gelatinases in climatic droplet keratopathy; Association for Research in Vision and Ophthalmology; Investigative Ophthalmology & Visual Science; 53; 7; 6-2012; 3527-35350146-04041552-5783CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pubmed/22570354info:eu-repo/semantics/altIdentifier/doi/10.1167/iovs.12-9441info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:01:56Zoai:ri.conicet.gov.ar:11336/55146instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:01:57.052CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Pro-inflammatory cytokines and gelatinases in climatic droplet keratopathy
title Pro-inflammatory cytokines and gelatinases in climatic droplet keratopathy
spellingShingle Pro-inflammatory cytokines and gelatinases in climatic droplet keratopathy
Holopainen, Juha M.
CYTOKINES
GELATINASES
CDK
CORNEA
title_short Pro-inflammatory cytokines and gelatinases in climatic droplet keratopathy
title_full Pro-inflammatory cytokines and gelatinases in climatic droplet keratopathy
title_fullStr Pro-inflammatory cytokines and gelatinases in climatic droplet keratopathy
title_full_unstemmed Pro-inflammatory cytokines and gelatinases in climatic droplet keratopathy
title_sort Pro-inflammatory cytokines and gelatinases in climatic droplet keratopathy
dc.creator.none.fl_str_mv Holopainen, Juha M.
Robciuc, Alexandra
Cafaro, Thamara Analia
Suarez, Maria Fernanda
Konttinen, Yrjö T.
Alkatan, Hind M.
Tabbara, Khalid F.
Tervahartiala, Taina
Sorsa, Timo
Urrets Zavalía, Julio Alberto
Serra, Horacio Marcelo
author Holopainen, Juha M.
author_facet Holopainen, Juha M.
Robciuc, Alexandra
Cafaro, Thamara Analia
Suarez, Maria Fernanda
Konttinen, Yrjö T.
Alkatan, Hind M.
Tabbara, Khalid F.
Tervahartiala, Taina
Sorsa, Timo
Urrets Zavalía, Julio Alberto
Serra, Horacio Marcelo
author_role author
author2 Robciuc, Alexandra
Cafaro, Thamara Analia
Suarez, Maria Fernanda
Konttinen, Yrjö T.
Alkatan, Hind M.
Tabbara, Khalid F.
Tervahartiala, Taina
Sorsa, Timo
Urrets Zavalía, Julio Alberto
Serra, Horacio Marcelo
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CYTOKINES
GELATINASES
CDK
CORNEA
topic CYTOKINES
GELATINASES
CDK
CORNEA
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv PURPOSE. Climatic droplet keratopathy (CDK) is a degenerative disease of the cornea with possible involvement from matrix metalloproteinases (MMPs). Therefore, the authors investigated histologic distribution, levels, and molecular forms of MMP- 2 and MMP-9, as well as tear fluid levels of MMPs and cytokines in CDK patients. They additionally examined UV-B-irradiation effect on production of gelatinases and cytokines by human corneal epithelial (HCE) cell culture model. METHODS. Tears were collected from 20 unrelated individuals (10 with CDK and 10 controls). CDK affected corneas were haematoxylin-eosin stained and the presence and distribution of MMP-2 and MMP-9 was examined using immunohistochemistry. Gelatinases and cytokine secretion was measured in tears and supernatants from UV-B-exposed HCEs by immunoblotting, gelatin zymography, and protein array, respectively. RESULTS. MMP-2 and MMP-9 values were significantly higher in tears collected from CDK patients than healthy controls and were accompanied by pro-inflammatory cytokine secretion. Immunohistochemistry showed that MMP-2 was expressed at the basement membrane zone in both control and affected corneas, but also marked the edges of the granular CDK deposits; MMP-9 expression was restrained to basal layers of the epithelium and was markedly induced in CDK corneas. In HCE cells, UV-B increased gelatinase secretion, with a striking effect on MMP-9, and was preceded by pro-inflammatory cytokine release. CONCLUSIONS. The authors demonstrate that the corneal epithelium could participate in CDK development as a source of cytokines and gelatinases. Additionally, in HCE cells, UV-B- modulated cytokine and subsequent MMP secretion. Local inhibition of cytokine secretion and gelatinases may prevent CDK progression. © 2012 The Association for Research in Vision and Ophthalmology, Inc.
Fil: Holopainen, Juha M.. University Of Helsinski; Finlandia
Fil: Robciuc, Alexandra. University Of Helsinski; Finlandia
Fil: Cafaro, Thamara Analia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Suarez, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Konttinen, Yrjö T.. University Of Helsinski; Finlandia
Fil: Alkatan, Hind M.. The Eye Center and The Eye Foundation for Research in Ophthalmology; Arabia Saudita
Fil: Tabbara, Khalid F.. The Eye Center and The Eye Foundation for Research in Ophthalmology; Arabia Saudita
Fil: Tervahartiala, Taina. University Of Helsinski; Finlandia
Fil: Sorsa, Timo. University Of Helsinski; Finlandia
Fil: Urrets Zavalía, Julio Alberto. Universidad Catolica de Córdoba. Facultad de Medicina. Clinica Universitaria Reina Fabiola. Dto de Oftalmologia; Argentina
Fil: Serra, Horacio Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
description PURPOSE. Climatic droplet keratopathy (CDK) is a degenerative disease of the cornea with possible involvement from matrix metalloproteinases (MMPs). Therefore, the authors investigated histologic distribution, levels, and molecular forms of MMP- 2 and MMP-9, as well as tear fluid levels of MMPs and cytokines in CDK patients. They additionally examined UV-B-irradiation effect on production of gelatinases and cytokines by human corneal epithelial (HCE) cell culture model. METHODS. Tears were collected from 20 unrelated individuals (10 with CDK and 10 controls). CDK affected corneas were haematoxylin-eosin stained and the presence and distribution of MMP-2 and MMP-9 was examined using immunohistochemistry. Gelatinases and cytokine secretion was measured in tears and supernatants from UV-B-exposed HCEs by immunoblotting, gelatin zymography, and protein array, respectively. RESULTS. MMP-2 and MMP-9 values were significantly higher in tears collected from CDK patients than healthy controls and were accompanied by pro-inflammatory cytokine secretion. Immunohistochemistry showed that MMP-2 was expressed at the basement membrane zone in both control and affected corneas, but also marked the edges of the granular CDK deposits; MMP-9 expression was restrained to basal layers of the epithelium and was markedly induced in CDK corneas. In HCE cells, UV-B increased gelatinase secretion, with a striking effect on MMP-9, and was preceded by pro-inflammatory cytokine release. CONCLUSIONS. The authors demonstrate that the corneal epithelium could participate in CDK development as a source of cytokines and gelatinases. Additionally, in HCE cells, UV-B- modulated cytokine and subsequent MMP secretion. Local inhibition of cytokine secretion and gelatinases may prevent CDK progression. © 2012 The Association for Research in Vision and Ophthalmology, Inc.
publishDate 2012
dc.date.none.fl_str_mv 2012-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/55146
Holopainen, Juha M.; Robciuc, Alexandra; Cafaro, Thamara Analia; Suarez, Maria Fernanda; Konttinen, Yrjö T. ; et al.; Pro-inflammatory cytokines and gelatinases in climatic droplet keratopathy; Association for Research in Vision and Ophthalmology; Investigative Ophthalmology & Visual Science; 53; 7; 6-2012; 3527-3535
0146-0404
1552-5783
CONICET Digital
CONICET
url http://hdl.handle.net/11336/55146
identifier_str_mv Holopainen, Juha M.; Robciuc, Alexandra; Cafaro, Thamara Analia; Suarez, Maria Fernanda; Konttinen, Yrjö T. ; et al.; Pro-inflammatory cytokines and gelatinases in climatic droplet keratopathy; Association for Research in Vision and Ophthalmology; Investigative Ophthalmology & Visual Science; 53; 7; 6-2012; 3527-3535
0146-0404
1552-5783
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pubmed/22570354
info:eu-repo/semantics/altIdentifier/doi/10.1167/iovs.12-9441
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Association for Research in Vision and Ophthalmology
publisher.none.fl_str_mv Association for Research in Vision and Ophthalmology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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