Pro-inflammatory cytokines and gelatinases in climatic droplet keratopathy
- Autores
- Holopainen, Juha M.; Robciuc, Alexandra; Cafaro, Thamara Analia; Suarez, Maria Fernanda; Konttinen, Yrjö T.; Alkatan, Hind M.; Tabbara, Khalid F.; Tervahartiala, Taina; Sorsa, Timo; Urrets Zavalía, Julio Alberto; Serra, Horacio Marcelo
- Año de publicación
- 2012
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- PURPOSE. Climatic droplet keratopathy (CDK) is a degenerative disease of the cornea with possible involvement from matrix metalloproteinases (MMPs). Therefore, the authors investigated histologic distribution, levels, and molecular forms of MMP- 2 and MMP-9, as well as tear fluid levels of MMPs and cytokines in CDK patients. They additionally examined UV-B-irradiation effect on production of gelatinases and cytokines by human corneal epithelial (HCE) cell culture model. METHODS. Tears were collected from 20 unrelated individuals (10 with CDK and 10 controls). CDK affected corneas were haematoxylin-eosin stained and the presence and distribution of MMP-2 and MMP-9 was examined using immunohistochemistry. Gelatinases and cytokine secretion was measured in tears and supernatants from UV-B-exposed HCEs by immunoblotting, gelatin zymography, and protein array, respectively. RESULTS. MMP-2 and MMP-9 values were significantly higher in tears collected from CDK patients than healthy controls and were accompanied by pro-inflammatory cytokine secretion. Immunohistochemistry showed that MMP-2 was expressed at the basement membrane zone in both control and affected corneas, but also marked the edges of the granular CDK deposits; MMP-9 expression was restrained to basal layers of the epithelium and was markedly induced in CDK corneas. In HCE cells, UV-B increased gelatinase secretion, with a striking effect on MMP-9, and was preceded by pro-inflammatory cytokine release. CONCLUSIONS. The authors demonstrate that the corneal epithelium could participate in CDK development as a source of cytokines and gelatinases. Additionally, in HCE cells, UV-B- modulated cytokine and subsequent MMP secretion. Local inhibition of cytokine secretion and gelatinases may prevent CDK progression. © 2012 The Association for Research in Vision and Ophthalmology, Inc.
Fil: Holopainen, Juha M.. University Of Helsinski; Finlandia
Fil: Robciuc, Alexandra. University Of Helsinski; Finlandia
Fil: Cafaro, Thamara Analia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Suarez, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Konttinen, Yrjö T.. University Of Helsinski; Finlandia
Fil: Alkatan, Hind M.. The Eye Center and The Eye Foundation for Research in Ophthalmology; Arabia Saudita
Fil: Tabbara, Khalid F.. The Eye Center and The Eye Foundation for Research in Ophthalmology; Arabia Saudita
Fil: Tervahartiala, Taina. University Of Helsinski; Finlandia
Fil: Sorsa, Timo. University Of Helsinski; Finlandia
Fil: Urrets Zavalía, Julio Alberto. Universidad Catolica de Córdoba. Facultad de Medicina. Clinica Universitaria Reina Fabiola. Dto de Oftalmologia; Argentina
Fil: Serra, Horacio Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina - Materia
-
CYTOKINES
GELATINASES
CDK
CORNEA - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/55146
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Pro-inflammatory cytokines and gelatinases in climatic droplet keratopathyHolopainen, Juha M.Robciuc, AlexandraCafaro, Thamara AnaliaSuarez, Maria FernandaKonttinen, Yrjö T.Alkatan, Hind M.Tabbara, Khalid F.Tervahartiala, TainaSorsa, TimoUrrets Zavalía, Julio AlbertoSerra, Horacio MarceloCYTOKINESGELATINASESCDKCORNEAhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3PURPOSE. Climatic droplet keratopathy (CDK) is a degenerative disease of the cornea with possible involvement from matrix metalloproteinases (MMPs). Therefore, the authors investigated histologic distribution, levels, and molecular forms of MMP- 2 and MMP-9, as well as tear fluid levels of MMPs and cytokines in CDK patients. They additionally examined UV-B-irradiation effect on production of gelatinases and cytokines by human corneal epithelial (HCE) cell culture model. METHODS. Tears were collected from 20 unrelated individuals (10 with CDK and 10 controls). CDK affected corneas were haematoxylin-eosin stained and the presence and distribution of MMP-2 and MMP-9 was examined using immunohistochemistry. Gelatinases and cytokine secretion was measured in tears and supernatants from UV-B-exposed HCEs by immunoblotting, gelatin zymography, and protein array, respectively. RESULTS. MMP-2 and MMP-9 values were significantly higher in tears collected from CDK patients than healthy controls and were accompanied by pro-inflammatory cytokine secretion. Immunohistochemistry showed that MMP-2 was expressed at the basement membrane zone in both control and affected corneas, but also marked the edges of the granular CDK deposits; MMP-9 expression was restrained to basal layers of the epithelium and was markedly induced in CDK corneas. In HCE cells, UV-B increased gelatinase secretion, with a striking effect on MMP-9, and was preceded by pro-inflammatory cytokine release. CONCLUSIONS. The authors demonstrate that the corneal epithelium could participate in CDK development as a source of cytokines and gelatinases. Additionally, in HCE cells, UV-B- modulated cytokine and subsequent MMP secretion. Local inhibition of cytokine secretion and gelatinases may prevent CDK progression. © 2012 The Association for Research in Vision and Ophthalmology, Inc.Fil: Holopainen, Juha M.. University Of Helsinski; FinlandiaFil: Robciuc, Alexandra. University Of Helsinski; FinlandiaFil: Cafaro, Thamara Analia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Suarez, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Konttinen, Yrjö T.. University Of Helsinski; FinlandiaFil: Alkatan, Hind M.. The Eye Center and The Eye Foundation for Research in Ophthalmology; Arabia SauditaFil: Tabbara, Khalid F.. The Eye Center and The Eye Foundation for Research in Ophthalmology; Arabia SauditaFil: Tervahartiala, Taina. University Of Helsinski; FinlandiaFil: Sorsa, Timo. University Of Helsinski; FinlandiaFil: Urrets Zavalía, Julio Alberto. Universidad Catolica de Córdoba. Facultad de Medicina. Clinica Universitaria Reina Fabiola. Dto de Oftalmologia; ArgentinaFil: Serra, Horacio Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaAssociation for Research in Vision and Ophthalmology2012-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/55146Holopainen, Juha M.; Robciuc, Alexandra; Cafaro, Thamara Analia; Suarez, Maria Fernanda; Konttinen, Yrjö T. ; et al.; Pro-inflammatory cytokines and gelatinases in climatic droplet keratopathy; Association for Research in Vision and Ophthalmology; Investigative Ophthalmology & Visual Science; 53; 7; 6-2012; 3527-35350146-04041552-5783CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pubmed/22570354info:eu-repo/semantics/altIdentifier/doi/10.1167/iovs.12-9441info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:05:17Zoai:ri.conicet.gov.ar:11336/55146instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:05:17.272CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Pro-inflammatory cytokines and gelatinases in climatic droplet keratopathy |
title |
Pro-inflammatory cytokines and gelatinases in climatic droplet keratopathy |
spellingShingle |
Pro-inflammatory cytokines and gelatinases in climatic droplet keratopathy Holopainen, Juha M. CYTOKINES GELATINASES CDK CORNEA |
title_short |
Pro-inflammatory cytokines and gelatinases in climatic droplet keratopathy |
title_full |
Pro-inflammatory cytokines and gelatinases in climatic droplet keratopathy |
title_fullStr |
Pro-inflammatory cytokines and gelatinases in climatic droplet keratopathy |
title_full_unstemmed |
Pro-inflammatory cytokines and gelatinases in climatic droplet keratopathy |
title_sort |
Pro-inflammatory cytokines and gelatinases in climatic droplet keratopathy |
dc.creator.none.fl_str_mv |
Holopainen, Juha M. Robciuc, Alexandra Cafaro, Thamara Analia Suarez, Maria Fernanda Konttinen, Yrjö T. Alkatan, Hind M. Tabbara, Khalid F. Tervahartiala, Taina Sorsa, Timo Urrets Zavalía, Julio Alberto Serra, Horacio Marcelo |
author |
Holopainen, Juha M. |
author_facet |
Holopainen, Juha M. Robciuc, Alexandra Cafaro, Thamara Analia Suarez, Maria Fernanda Konttinen, Yrjö T. Alkatan, Hind M. Tabbara, Khalid F. Tervahartiala, Taina Sorsa, Timo Urrets Zavalía, Julio Alberto Serra, Horacio Marcelo |
author_role |
author |
author2 |
Robciuc, Alexandra Cafaro, Thamara Analia Suarez, Maria Fernanda Konttinen, Yrjö T. Alkatan, Hind M. Tabbara, Khalid F. Tervahartiala, Taina Sorsa, Timo Urrets Zavalía, Julio Alberto Serra, Horacio Marcelo |
author2_role |
author author author author author author author author author author |
dc.subject.none.fl_str_mv |
CYTOKINES GELATINASES CDK CORNEA |
topic |
CYTOKINES GELATINASES CDK CORNEA |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
PURPOSE. Climatic droplet keratopathy (CDK) is a degenerative disease of the cornea with possible involvement from matrix metalloproteinases (MMPs). Therefore, the authors investigated histologic distribution, levels, and molecular forms of MMP- 2 and MMP-9, as well as tear fluid levels of MMPs and cytokines in CDK patients. They additionally examined UV-B-irradiation effect on production of gelatinases and cytokines by human corneal epithelial (HCE) cell culture model. METHODS. Tears were collected from 20 unrelated individuals (10 with CDK and 10 controls). CDK affected corneas were haematoxylin-eosin stained and the presence and distribution of MMP-2 and MMP-9 was examined using immunohistochemistry. Gelatinases and cytokine secretion was measured in tears and supernatants from UV-B-exposed HCEs by immunoblotting, gelatin zymography, and protein array, respectively. RESULTS. MMP-2 and MMP-9 values were significantly higher in tears collected from CDK patients than healthy controls and were accompanied by pro-inflammatory cytokine secretion. Immunohistochemistry showed that MMP-2 was expressed at the basement membrane zone in both control and affected corneas, but also marked the edges of the granular CDK deposits; MMP-9 expression was restrained to basal layers of the epithelium and was markedly induced in CDK corneas. In HCE cells, UV-B increased gelatinase secretion, with a striking effect on MMP-9, and was preceded by pro-inflammatory cytokine release. CONCLUSIONS. The authors demonstrate that the corneal epithelium could participate in CDK development as a source of cytokines and gelatinases. Additionally, in HCE cells, UV-B- modulated cytokine and subsequent MMP secretion. Local inhibition of cytokine secretion and gelatinases may prevent CDK progression. © 2012 The Association for Research in Vision and Ophthalmology, Inc. Fil: Holopainen, Juha M.. University Of Helsinski; Finlandia Fil: Robciuc, Alexandra. University Of Helsinski; Finlandia Fil: Cafaro, Thamara Analia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina Fil: Suarez, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina Fil: Konttinen, Yrjö T.. University Of Helsinski; Finlandia Fil: Alkatan, Hind M.. The Eye Center and The Eye Foundation for Research in Ophthalmology; Arabia Saudita Fil: Tabbara, Khalid F.. The Eye Center and The Eye Foundation for Research in Ophthalmology; Arabia Saudita Fil: Tervahartiala, Taina. University Of Helsinski; Finlandia Fil: Sorsa, Timo. University Of Helsinski; Finlandia Fil: Urrets Zavalía, Julio Alberto. Universidad Catolica de Córdoba. Facultad de Medicina. Clinica Universitaria Reina Fabiola. Dto de Oftalmologia; Argentina Fil: Serra, Horacio Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina |
description |
PURPOSE. Climatic droplet keratopathy (CDK) is a degenerative disease of the cornea with possible involvement from matrix metalloproteinases (MMPs). Therefore, the authors investigated histologic distribution, levels, and molecular forms of MMP- 2 and MMP-9, as well as tear fluid levels of MMPs and cytokines in CDK patients. They additionally examined UV-B-irradiation effect on production of gelatinases and cytokines by human corneal epithelial (HCE) cell culture model. METHODS. Tears were collected from 20 unrelated individuals (10 with CDK and 10 controls). CDK affected corneas were haematoxylin-eosin stained and the presence and distribution of MMP-2 and MMP-9 was examined using immunohistochemistry. Gelatinases and cytokine secretion was measured in tears and supernatants from UV-B-exposed HCEs by immunoblotting, gelatin zymography, and protein array, respectively. RESULTS. MMP-2 and MMP-9 values were significantly higher in tears collected from CDK patients than healthy controls and were accompanied by pro-inflammatory cytokine secretion. Immunohistochemistry showed that MMP-2 was expressed at the basement membrane zone in both control and affected corneas, but also marked the edges of the granular CDK deposits; MMP-9 expression was restrained to basal layers of the epithelium and was markedly induced in CDK corneas. In HCE cells, UV-B increased gelatinase secretion, with a striking effect on MMP-9, and was preceded by pro-inflammatory cytokine release. CONCLUSIONS. The authors demonstrate that the corneal epithelium could participate in CDK development as a source of cytokines and gelatinases. Additionally, in HCE cells, UV-B- modulated cytokine and subsequent MMP secretion. Local inhibition of cytokine secretion and gelatinases may prevent CDK progression. © 2012 The Association for Research in Vision and Ophthalmology, Inc. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-06 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/55146 Holopainen, Juha M.; Robciuc, Alexandra; Cafaro, Thamara Analia; Suarez, Maria Fernanda; Konttinen, Yrjö T. ; et al.; Pro-inflammatory cytokines and gelatinases in climatic droplet keratopathy; Association for Research in Vision and Ophthalmology; Investigative Ophthalmology & Visual Science; 53; 7; 6-2012; 3527-3535 0146-0404 1552-5783 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/55146 |
identifier_str_mv |
Holopainen, Juha M.; Robciuc, Alexandra; Cafaro, Thamara Analia; Suarez, Maria Fernanda; Konttinen, Yrjö T. ; et al.; Pro-inflammatory cytokines and gelatinases in climatic droplet keratopathy; Association for Research in Vision and Ophthalmology; Investigative Ophthalmology & Visual Science; 53; 7; 6-2012; 3527-3535 0146-0404 1552-5783 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pubmed/22570354 info:eu-repo/semantics/altIdentifier/doi/10.1167/iovs.12-9441 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Association for Research in Vision and Ophthalmology |
publisher.none.fl_str_mv |
Association for Research in Vision and Ophthalmology |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613887205310464 |
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13.070432 |