Phospholipidomic Studies in Human Cornea From Climatic Droplet Keratopathy
- Autores
- Suarez, Maria Fernanda; Piqueras, Maria Carmen; Correa, Leandro Javier; Esposito, Evangelina; Barros Centeno, María Fernanda; Bhattacharya, Sanjoy K.; Urrets Zavalía, Julio Alberto; Serra, Horacio Marcelo
- Año de publicación
- 2017
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Climatic droplet keratopathy (CDK) is an acquired degenerative disease predominantly affecting males over 40 years old. It results in progressive corneal opacities usually affecting both eyes. CDK is multifactorial and its etiology remains unknown. Our recent findings are consistent with CDK pathology being driven by environmental factors with oxidative stress playing an important role (e.g.,, contributing to lipid peroxidation) rather than climate factors. The changes in corneal lipid composition affected by environmental factors remain understudied. The purpose of this study was to systematically investigate phospholipids profile (phosphatidylcholine [PC] and phosphatidylserine [PS]) in corneas from CDK patients using tandem mass spectrometry. Samples from CDK areas and from non-affected areas were obtained from patients diagnosed with CDK who underwent cataract surgery, were subjected to lipid extraction using a modified Bligh and Dyer method; protein concentrations were determined using the Bradford's method. Lipids were identified and subjected to ratiometric quantification using TSQ Quantum Access Max triple quadrupole mass spectrometer, using appropriate class specific lipid standards. All phospholipid classes showed lower total amounts in affected areas compared to control areas from CDK's corneas. Comparative profiles of two phospholipid classes (PC, PS) between CDK areas and control areas showed several common species between them. We also found a few unique lipids that were absent in CDK areas compared to controls and vice versa. Lower amount of phospholipids in CDK areas compared to control areas could be attributed to the lipid peroxidation in the affected corneal regions as a consequence of increased oxidative stress.
Fil: Suarez, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina
Fil: Piqueras, Maria Carmen. University of Miami; Estados Unidos
Fil: Correa, Leandro Javier. Universidad Católica de Córdoba. Facultad de Medicina. Clínica Universitaria Reina Fabiola; Argentina
Fil: Esposito, Evangelina. Universidad Católica de Córdoba. Facultad de Medicina. Clínica Universitaria Reina Fabiola; Argentina
Fil: Barros Centeno, María Fernanda. Universidad Católica de Córdoba. Facultad de Medicina. Clínica Universitaria Reina Fabiola; Argentina
Fil: Bhattacharya, Sanjoy K.. University of Miami; Estados Unidos
Fil: Urrets Zavalía, Julio Alberto. Universidad Católica de Córdoba. Facultad de Medicina. Clínica Universitaria Reina Fabiola; Argentina
Fil: Serra, Horacio Marcelo. Universidad Nacional de Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina - Materia
-
CDK
CORNEA
PHOSPHATIDYLCHOLINE
PHOSPHATIDYLSERINE
PHOSPHOLIPIDS
TANDEM MASS SPECTROMETRY - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/85766
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Phospholipidomic Studies in Human Cornea From Climatic Droplet KeratopathySuarez, Maria FernandaPiqueras, Maria CarmenCorrea, Leandro JavierEsposito, EvangelinaBarros Centeno, María FernandaBhattacharya, Sanjoy K.Urrets Zavalía, Julio AlbertoSerra, Horacio MarceloCDKCORNEAPHOSPHATIDYLCHOLINEPHOSPHATIDYLSERINEPHOSPHOLIPIDSTANDEM MASS SPECTROMETRYhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Climatic droplet keratopathy (CDK) is an acquired degenerative disease predominantly affecting males over 40 years old. It results in progressive corneal opacities usually affecting both eyes. CDK is multifactorial and its etiology remains unknown. Our recent findings are consistent with CDK pathology being driven by environmental factors with oxidative stress playing an important role (e.g.,, contributing to lipid peroxidation) rather than climate factors. The changes in corneal lipid composition affected by environmental factors remain understudied. The purpose of this study was to systematically investigate phospholipids profile (phosphatidylcholine [PC] and phosphatidylserine [PS]) in corneas from CDK patients using tandem mass spectrometry. Samples from CDK areas and from non-affected areas were obtained from patients diagnosed with CDK who underwent cataract surgery, were subjected to lipid extraction using a modified Bligh and Dyer method; protein concentrations were determined using the Bradford's method. Lipids were identified and subjected to ratiometric quantification using TSQ Quantum Access Max triple quadrupole mass spectrometer, using appropriate class specific lipid standards. All phospholipid classes showed lower total amounts in affected areas compared to control areas from CDK's corneas. Comparative profiles of two phospholipid classes (PC, PS) between CDK areas and control areas showed several common species between them. We also found a few unique lipids that were absent in CDK areas compared to controls and vice versa. Lower amount of phospholipids in CDK areas compared to control areas could be attributed to the lipid peroxidation in the affected corneal regions as a consequence of increased oxidative stress.Fil: Suarez, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; ArgentinaFil: Piqueras, Maria Carmen. University of Miami; Estados UnidosFil: Correa, Leandro Javier. Universidad Católica de Córdoba. Facultad de Medicina. Clínica Universitaria Reina Fabiola; ArgentinaFil: Esposito, Evangelina. Universidad Católica de Córdoba. Facultad de Medicina. Clínica Universitaria Reina Fabiola; ArgentinaFil: Barros Centeno, María Fernanda. Universidad Católica de Córdoba. Facultad de Medicina. Clínica Universitaria Reina Fabiola; ArgentinaFil: Bhattacharya, Sanjoy K.. University of Miami; Estados UnidosFil: Urrets Zavalía, Julio Alberto. Universidad Católica de Córdoba. Facultad de Medicina. Clínica Universitaria Reina Fabiola; ArgentinaFil: Serra, Horacio Marcelo. Universidad Nacional de Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; ArgentinaWiley-liss, Div John Wiley & Sons Inc2017-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/85766Suarez, Maria Fernanda; Piqueras, Maria Carmen; Correa, Leandro Javier; Esposito, Evangelina; Barros Centeno, María Fernanda; et al.; Phospholipidomic Studies in Human Cornea From Climatic Droplet Keratopathy; Wiley-liss, Div John Wiley & Sons Inc; Journal of Cellular Biochemistry; 118; 11; 11-2017; 3920-39310730-2312CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/jcb.26045info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/jcb.26045info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5603377/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:06:59Zoai:ri.conicet.gov.ar:11336/85766instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:06:59.58CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Phospholipidomic Studies in Human Cornea From Climatic Droplet Keratopathy |
title |
Phospholipidomic Studies in Human Cornea From Climatic Droplet Keratopathy |
spellingShingle |
Phospholipidomic Studies in Human Cornea From Climatic Droplet Keratopathy Suarez, Maria Fernanda CDK CORNEA PHOSPHATIDYLCHOLINE PHOSPHATIDYLSERINE PHOSPHOLIPIDS TANDEM MASS SPECTROMETRY |
title_short |
Phospholipidomic Studies in Human Cornea From Climatic Droplet Keratopathy |
title_full |
Phospholipidomic Studies in Human Cornea From Climatic Droplet Keratopathy |
title_fullStr |
Phospholipidomic Studies in Human Cornea From Climatic Droplet Keratopathy |
title_full_unstemmed |
Phospholipidomic Studies in Human Cornea From Climatic Droplet Keratopathy |
title_sort |
Phospholipidomic Studies in Human Cornea From Climatic Droplet Keratopathy |
dc.creator.none.fl_str_mv |
Suarez, Maria Fernanda Piqueras, Maria Carmen Correa, Leandro Javier Esposito, Evangelina Barros Centeno, María Fernanda Bhattacharya, Sanjoy K. Urrets Zavalía, Julio Alberto Serra, Horacio Marcelo |
author |
Suarez, Maria Fernanda |
author_facet |
Suarez, Maria Fernanda Piqueras, Maria Carmen Correa, Leandro Javier Esposito, Evangelina Barros Centeno, María Fernanda Bhattacharya, Sanjoy K. Urrets Zavalía, Julio Alberto Serra, Horacio Marcelo |
author_role |
author |
author2 |
Piqueras, Maria Carmen Correa, Leandro Javier Esposito, Evangelina Barros Centeno, María Fernanda Bhattacharya, Sanjoy K. Urrets Zavalía, Julio Alberto Serra, Horacio Marcelo |
author2_role |
author author author author author author author |
dc.subject.none.fl_str_mv |
CDK CORNEA PHOSPHATIDYLCHOLINE PHOSPHATIDYLSERINE PHOSPHOLIPIDS TANDEM MASS SPECTROMETRY |
topic |
CDK CORNEA PHOSPHATIDYLCHOLINE PHOSPHATIDYLSERINE PHOSPHOLIPIDS TANDEM MASS SPECTROMETRY |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.3 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
Climatic droplet keratopathy (CDK) is an acquired degenerative disease predominantly affecting males over 40 years old. It results in progressive corneal opacities usually affecting both eyes. CDK is multifactorial and its etiology remains unknown. Our recent findings are consistent with CDK pathology being driven by environmental factors with oxidative stress playing an important role (e.g.,, contributing to lipid peroxidation) rather than climate factors. The changes in corneal lipid composition affected by environmental factors remain understudied. The purpose of this study was to systematically investigate phospholipids profile (phosphatidylcholine [PC] and phosphatidylserine [PS]) in corneas from CDK patients using tandem mass spectrometry. Samples from CDK areas and from non-affected areas were obtained from patients diagnosed with CDK who underwent cataract surgery, were subjected to lipid extraction using a modified Bligh and Dyer method; protein concentrations were determined using the Bradford's method. Lipids were identified and subjected to ratiometric quantification using TSQ Quantum Access Max triple quadrupole mass spectrometer, using appropriate class specific lipid standards. All phospholipid classes showed lower total amounts in affected areas compared to control areas from CDK's corneas. Comparative profiles of two phospholipid classes (PC, PS) between CDK areas and control areas showed several common species between them. We also found a few unique lipids that were absent in CDK areas compared to controls and vice versa. Lower amount of phospholipids in CDK areas compared to control areas could be attributed to the lipid peroxidation in the affected corneal regions as a consequence of increased oxidative stress. Fil: Suarez, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina Fil: Piqueras, Maria Carmen. University of Miami; Estados Unidos Fil: Correa, Leandro Javier. Universidad Católica de Córdoba. Facultad de Medicina. Clínica Universitaria Reina Fabiola; Argentina Fil: Esposito, Evangelina. Universidad Católica de Córdoba. Facultad de Medicina. Clínica Universitaria Reina Fabiola; Argentina Fil: Barros Centeno, María Fernanda. Universidad Católica de Córdoba. Facultad de Medicina. Clínica Universitaria Reina Fabiola; Argentina Fil: Bhattacharya, Sanjoy K.. University of Miami; Estados Unidos Fil: Urrets Zavalía, Julio Alberto. Universidad Católica de Córdoba. Facultad de Medicina. Clínica Universitaria Reina Fabiola; Argentina Fil: Serra, Horacio Marcelo. Universidad Nacional de Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina |
description |
Climatic droplet keratopathy (CDK) is an acquired degenerative disease predominantly affecting males over 40 years old. It results in progressive corneal opacities usually affecting both eyes. CDK is multifactorial and its etiology remains unknown. Our recent findings are consistent with CDK pathology being driven by environmental factors with oxidative stress playing an important role (e.g.,, contributing to lipid peroxidation) rather than climate factors. The changes in corneal lipid composition affected by environmental factors remain understudied. The purpose of this study was to systematically investigate phospholipids profile (phosphatidylcholine [PC] and phosphatidylserine [PS]) in corneas from CDK patients using tandem mass spectrometry. Samples from CDK areas and from non-affected areas were obtained from patients diagnosed with CDK who underwent cataract surgery, were subjected to lipid extraction using a modified Bligh and Dyer method; protein concentrations were determined using the Bradford's method. Lipids were identified and subjected to ratiometric quantification using TSQ Quantum Access Max triple quadrupole mass spectrometer, using appropriate class specific lipid standards. All phospholipid classes showed lower total amounts in affected areas compared to control areas from CDK's corneas. Comparative profiles of two phospholipid classes (PC, PS) between CDK areas and control areas showed several common species between them. We also found a few unique lipids that were absent in CDK areas compared to controls and vice versa. Lower amount of phospholipids in CDK areas compared to control areas could be attributed to the lipid peroxidation in the affected corneal regions as a consequence of increased oxidative stress. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-11 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/85766 Suarez, Maria Fernanda; Piqueras, Maria Carmen; Correa, Leandro Javier; Esposito, Evangelina; Barros Centeno, María Fernanda; et al.; Phospholipidomic Studies in Human Cornea From Climatic Droplet Keratopathy; Wiley-liss, Div John Wiley & Sons Inc; Journal of Cellular Biochemistry; 118; 11; 11-2017; 3920-3931 0730-2312 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/85766 |
identifier_str_mv |
Suarez, Maria Fernanda; Piqueras, Maria Carmen; Correa, Leandro Javier; Esposito, Evangelina; Barros Centeno, María Fernanda; et al.; Phospholipidomic Studies in Human Cornea From Climatic Droplet Keratopathy; Wiley-liss, Div John Wiley & Sons Inc; Journal of Cellular Biochemistry; 118; 11; 11-2017; 3920-3931 0730-2312 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1002/jcb.26045 info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/jcb.26045 info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5603377/ |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Wiley-liss, Div John Wiley & Sons Inc |
publisher.none.fl_str_mv |
Wiley-liss, Div John Wiley & Sons Inc |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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