Phospholipidomic Studies in Human Cornea From Climatic Droplet Keratopathy

Autores
Suarez, Maria Fernanda; Piqueras, Maria Carmen; Correa, Leandro Javier; Esposito, Evangelina; Barros Centeno, María Fernanda; Bhattacharya, Sanjoy K.; Urrets Zavalía, Julio Alberto; Serra, Horacio Marcelo
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Climatic droplet keratopathy (CDK) is an acquired degenerative disease predominantly affecting males over 40 years old. It results in progressive corneal opacities usually affecting both eyes. CDK is multifactorial and its etiology remains unknown. Our recent findings are consistent with CDK pathology being driven by environmental factors with oxidative stress playing an important role (e.g.,, contributing to lipid peroxidation) rather than climate factors. The changes in corneal lipid composition affected by environmental factors remain understudied. The purpose of this study was to systematically investigate phospholipids profile (phosphatidylcholine [PC] and phosphatidylserine [PS]) in corneas from CDK patients using tandem mass spectrometry. Samples from CDK areas and from non-affected areas were obtained from patients diagnosed with CDK who underwent cataract surgery, were subjected to lipid extraction using a modified Bligh and Dyer method; protein concentrations were determined using the Bradford's method. Lipids were identified and subjected to ratiometric quantification using TSQ Quantum Access Max triple quadrupole mass spectrometer, using appropriate class specific lipid standards. All phospholipid classes showed lower total amounts in affected areas compared to control areas from CDK's corneas. Comparative profiles of two phospholipid classes (PC, PS) between CDK areas and control areas showed several common species between them. We also found a few unique lipids that were absent in CDK areas compared to controls and vice versa. Lower amount of phospholipids in CDK areas compared to control areas could be attributed to the lipid peroxidation in the affected corneal regions as a consequence of increased oxidative stress.
Fil: Suarez, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina
Fil: Piqueras, Maria Carmen. University of Miami; Estados Unidos
Fil: Correa, Leandro Javier. Universidad Católica de Córdoba. Facultad de Medicina. Clínica Universitaria Reina Fabiola; Argentina
Fil: Esposito, Evangelina. Universidad Católica de Córdoba. Facultad de Medicina. Clínica Universitaria Reina Fabiola; Argentina
Fil: Barros Centeno, María Fernanda. Universidad Católica de Córdoba. Facultad de Medicina. Clínica Universitaria Reina Fabiola; Argentina
Fil: Bhattacharya, Sanjoy K.. University of Miami; Estados Unidos
Fil: Urrets Zavalía, Julio Alberto. Universidad Católica de Córdoba. Facultad de Medicina. Clínica Universitaria Reina Fabiola; Argentina
Fil: Serra, Horacio Marcelo. Universidad Nacional de Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina
Materia
CDK
CORNEA
PHOSPHATIDYLCHOLINE
PHOSPHATIDYLSERINE
PHOSPHOLIPIDS
TANDEM MASS SPECTROMETRY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/85766
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/85766
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Phospholipidomic Studies in Human Cornea From Climatic Droplet KeratopathySuarez, Maria FernandaPiqueras, Maria CarmenCorrea, Leandro JavierEsposito, EvangelinaBarros Centeno, María FernandaBhattacharya, Sanjoy K.Urrets Zavalía, Julio AlbertoSerra, Horacio MarceloCDKCORNEAPHOSPHATIDYLCHOLINEPHOSPHATIDYLSERINEPHOSPHOLIPIDSTANDEM MASS SPECTROMETRYhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Climatic droplet keratopathy (CDK) is an acquired degenerative disease predominantly affecting males over 40 years old. It results in progressive corneal opacities usually affecting both eyes. CDK is multifactorial and its etiology remains unknown. Our recent findings are consistent with CDK pathology being driven by environmental factors with oxidative stress playing an important role (e.g.,, contributing to lipid peroxidation) rather than climate factors. The changes in corneal lipid composition affected by environmental factors remain understudied. The purpose of this study was to systematically investigate phospholipids profile (phosphatidylcholine [PC] and phosphatidylserine [PS]) in corneas from CDK patients using tandem mass spectrometry. Samples from CDK areas and from non-affected areas were obtained from patients diagnosed with CDK who underwent cataract surgery, were subjected to lipid extraction using a modified Bligh and Dyer method; protein concentrations were determined using the Bradford's method. Lipids were identified and subjected to ratiometric quantification using TSQ Quantum Access Max triple quadrupole mass spectrometer, using appropriate class specific lipid standards. All phospholipid classes showed lower total amounts in affected areas compared to control areas from CDK's corneas. Comparative profiles of two phospholipid classes (PC, PS) between CDK areas and control areas showed several common species between them. We also found a few unique lipids that were absent in CDK areas compared to controls and vice versa. Lower amount of phospholipids in CDK areas compared to control areas could be attributed to the lipid peroxidation in the affected corneal regions as a consequence of increased oxidative stress.Fil: Suarez, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; ArgentinaFil: Piqueras, Maria Carmen. University of Miami; Estados UnidosFil: Correa, Leandro Javier. Universidad Católica de Córdoba. Facultad de Medicina. Clínica Universitaria Reina Fabiola; ArgentinaFil: Esposito, Evangelina. Universidad Católica de Córdoba. Facultad de Medicina. Clínica Universitaria Reina Fabiola; ArgentinaFil: Barros Centeno, María Fernanda. Universidad Católica de Córdoba. Facultad de Medicina. Clínica Universitaria Reina Fabiola; ArgentinaFil: Bhattacharya, Sanjoy K.. University of Miami; Estados UnidosFil: Urrets Zavalía, Julio Alberto. Universidad Católica de Córdoba. Facultad de Medicina. Clínica Universitaria Reina Fabiola; ArgentinaFil: Serra, Horacio Marcelo. Universidad Nacional de Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; ArgentinaWiley-liss, Div John Wiley & Sons Inc2017-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/85766Suarez, Maria Fernanda; Piqueras, Maria Carmen; Correa, Leandro Javier; Esposito, Evangelina; Barros Centeno, María Fernanda; et al.; Phospholipidomic Studies in Human Cornea From Climatic Droplet Keratopathy; Wiley-liss, Div John Wiley & Sons Inc; Journal of Cellular Biochemistry; 118; 11; 11-2017; 3920-39310730-2312CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/jcb.26045info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/jcb.26045info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5603377/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:03:54Zoai:ri.conicet.gov.ar:11336/85766instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:03:54.811CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Phospholipidomic Studies in Human Cornea From Climatic Droplet Keratopathy
title Phospholipidomic Studies in Human Cornea From Climatic Droplet Keratopathy
spellingShingle Phospholipidomic Studies in Human Cornea From Climatic Droplet Keratopathy
Suarez, Maria Fernanda
CDK
CORNEA
PHOSPHATIDYLCHOLINE
PHOSPHATIDYLSERINE
PHOSPHOLIPIDS
TANDEM MASS SPECTROMETRY
title_short Phospholipidomic Studies in Human Cornea From Climatic Droplet Keratopathy
title_full Phospholipidomic Studies in Human Cornea From Climatic Droplet Keratopathy
title_fullStr Phospholipidomic Studies in Human Cornea From Climatic Droplet Keratopathy
title_full_unstemmed Phospholipidomic Studies in Human Cornea From Climatic Droplet Keratopathy
title_sort Phospholipidomic Studies in Human Cornea From Climatic Droplet Keratopathy
dc.creator.none.fl_str_mv Suarez, Maria Fernanda
Piqueras, Maria Carmen
Correa, Leandro Javier
Esposito, Evangelina
Barros Centeno, María Fernanda
Bhattacharya, Sanjoy K.
Urrets Zavalía, Julio Alberto
Serra, Horacio Marcelo
author Suarez, Maria Fernanda
author_facet Suarez, Maria Fernanda
Piqueras, Maria Carmen
Correa, Leandro Javier
Esposito, Evangelina
Barros Centeno, María Fernanda
Bhattacharya, Sanjoy K.
Urrets Zavalía, Julio Alberto
Serra, Horacio Marcelo
author_role author
author2 Piqueras, Maria Carmen
Correa, Leandro Javier
Esposito, Evangelina
Barros Centeno, María Fernanda
Bhattacharya, Sanjoy K.
Urrets Zavalía, Julio Alberto
Serra, Horacio Marcelo
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CDK
CORNEA
PHOSPHATIDYLCHOLINE
PHOSPHATIDYLSERINE
PHOSPHOLIPIDS
TANDEM MASS SPECTROMETRY
topic CDK
CORNEA
PHOSPHATIDYLCHOLINE
PHOSPHATIDYLSERINE
PHOSPHOLIPIDS
TANDEM MASS SPECTROMETRY
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Climatic droplet keratopathy (CDK) is an acquired degenerative disease predominantly affecting males over 40 years old. It results in progressive corneal opacities usually affecting both eyes. CDK is multifactorial and its etiology remains unknown. Our recent findings are consistent with CDK pathology being driven by environmental factors with oxidative stress playing an important role (e.g.,, contributing to lipid peroxidation) rather than climate factors. The changes in corneal lipid composition affected by environmental factors remain understudied. The purpose of this study was to systematically investigate phospholipids profile (phosphatidylcholine [PC] and phosphatidylserine [PS]) in corneas from CDK patients using tandem mass spectrometry. Samples from CDK areas and from non-affected areas were obtained from patients diagnosed with CDK who underwent cataract surgery, were subjected to lipid extraction using a modified Bligh and Dyer method; protein concentrations were determined using the Bradford's method. Lipids were identified and subjected to ratiometric quantification using TSQ Quantum Access Max triple quadrupole mass spectrometer, using appropriate class specific lipid standards. All phospholipid classes showed lower total amounts in affected areas compared to control areas from CDK's corneas. Comparative profiles of two phospholipid classes (PC, PS) between CDK areas and control areas showed several common species between them. We also found a few unique lipids that were absent in CDK areas compared to controls and vice versa. Lower amount of phospholipids in CDK areas compared to control areas could be attributed to the lipid peroxidation in the affected corneal regions as a consequence of increased oxidative stress.
Fil: Suarez, Maria Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina
Fil: Piqueras, Maria Carmen. University of Miami; Estados Unidos
Fil: Correa, Leandro Javier. Universidad Católica de Córdoba. Facultad de Medicina. Clínica Universitaria Reina Fabiola; Argentina
Fil: Esposito, Evangelina. Universidad Católica de Córdoba. Facultad de Medicina. Clínica Universitaria Reina Fabiola; Argentina
Fil: Barros Centeno, María Fernanda. Universidad Católica de Córdoba. Facultad de Medicina. Clínica Universitaria Reina Fabiola; Argentina
Fil: Bhattacharya, Sanjoy K.. University of Miami; Estados Unidos
Fil: Urrets Zavalía, Julio Alberto. Universidad Católica de Córdoba. Facultad de Medicina. Clínica Universitaria Reina Fabiola; Argentina
Fil: Serra, Horacio Marcelo. Universidad Nacional de Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica; Argentina
description Climatic droplet keratopathy (CDK) is an acquired degenerative disease predominantly affecting males over 40 years old. It results in progressive corneal opacities usually affecting both eyes. CDK is multifactorial and its etiology remains unknown. Our recent findings are consistent with CDK pathology being driven by environmental factors with oxidative stress playing an important role (e.g.,, contributing to lipid peroxidation) rather than climate factors. The changes in corneal lipid composition affected by environmental factors remain understudied. The purpose of this study was to systematically investigate phospholipids profile (phosphatidylcholine [PC] and phosphatidylserine [PS]) in corneas from CDK patients using tandem mass spectrometry. Samples from CDK areas and from non-affected areas were obtained from patients diagnosed with CDK who underwent cataract surgery, were subjected to lipid extraction using a modified Bligh and Dyer method; protein concentrations were determined using the Bradford's method. Lipids were identified and subjected to ratiometric quantification using TSQ Quantum Access Max triple quadrupole mass spectrometer, using appropriate class specific lipid standards. All phospholipid classes showed lower total amounts in affected areas compared to control areas from CDK's corneas. Comparative profiles of two phospholipid classes (PC, PS) between CDK areas and control areas showed several common species between them. We also found a few unique lipids that were absent in CDK areas compared to controls and vice versa. Lower amount of phospholipids in CDK areas compared to control areas could be attributed to the lipid peroxidation in the affected corneal regions as a consequence of increased oxidative stress.
publishDate 2017
dc.date.none.fl_str_mv 2017-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/85766
Suarez, Maria Fernanda; Piqueras, Maria Carmen; Correa, Leandro Javier; Esposito, Evangelina; Barros Centeno, María Fernanda; et al.; Phospholipidomic Studies in Human Cornea From Climatic Droplet Keratopathy; Wiley-liss, Div John Wiley & Sons Inc; Journal of Cellular Biochemistry; 118; 11; 11-2017; 3920-3931
0730-2312
CONICET Digital
CONICET
url http://hdl.handle.net/11336/85766
identifier_str_mv Suarez, Maria Fernanda; Piqueras, Maria Carmen; Correa, Leandro Javier; Esposito, Evangelina; Barros Centeno, María Fernanda; et al.; Phospholipidomic Studies in Human Cornea From Climatic Droplet Keratopathy; Wiley-liss, Div John Wiley & Sons Inc; Journal of Cellular Biochemistry; 118; 11; 11-2017; 3920-3931
0730-2312
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/jcb.26045
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/jcb.26045
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5603377/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley-liss, Div John Wiley & Sons Inc
publisher.none.fl_str_mv Wiley-liss, Div John Wiley & Sons Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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