An accurate UV/visible method to quantify proteins and nzymes:Impact of aggregation, buffer concentration and the nature of the standard

Autores
Lassalle, Verónica Leticia; Pirillo, Silvina; Rueda, Elsa Haydee; Ferreira, María Luján
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A simple, fast and low cost UV/visible based method to quantify proteins or enzymes is presented. This method avoids some drawbacks found using conventional techniques. Representative proteins and enzymes such as bovine serum albumin (BSA), insulin (Ins.), Rhizomucor meihei lipase (RML), Candida rugosa lipase (CRL) and horseradish peroxidase (HRP) were assayed as model. Experiments revealed that the aggregation of the enzyme/protein molecules in aqueous solution was the main cause of inaccurate results obtained with the simple UV/visible method. It was determined that aggregation of proteins/ enzymes in aqueous solutions follows a reversible mechanism that could be reverted by simple magnetic stirring treatment. The results achieved within this study warn about common error sources in protein quantification by UV/visible based methods and clearly shows the magnitude of the mistakes that could be achieved if aggregation and other factors are not considered.
Fil: Lassalle, Verónica Leticia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Pirillo, Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Rueda, Elsa Haydee. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Ferreira, María Luján. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina
Materia
Enzymes
Biocatalyst
Adsorption
Proteins
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/64696
Acceder
id CONICETDig_a0f90da849c6735d9da894354328a5b2
oai_identifier_str oai:ri.conicet.gov.ar:11336/64696
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling An accurate UV/visible method to quantify proteins and nzymes:Impact of aggregation, buffer concentration and the nature of the standardLassalle, Verónica LeticiaPirillo, SilvinaRueda, Elsa HaydeeFerreira, María LujánEnzymesBiocatalystAdsorptionProteinshttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2A simple, fast and low cost UV/visible based method to quantify proteins or enzymes is presented. This method avoids some drawbacks found using conventional techniques. Representative proteins and enzymes such as bovine serum albumin (BSA), insulin (Ins.), Rhizomucor meihei lipase (RML), Candida rugosa lipase (CRL) and horseradish peroxidase (HRP) were assayed as model. Experiments revealed that the aggregation of the enzyme/protein molecules in aqueous solution was the main cause of inaccurate results obtained with the simple UV/visible method. It was determined that aggregation of proteins/ enzymes in aqueous solutions follows a reversible mechanism that could be reverted by simple magnetic stirring treatment. The results achieved within this study warn about common error sources in protein quantification by UV/visible based methods and clearly shows the magnitude of the mistakes that could be achieved if aggregation and other factors are not considered.Fil: Lassalle, Verónica Leticia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; ArgentinaFil: Pirillo, Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; ArgentinaFil: Rueda, Elsa Haydee. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; ArgentinaFil: Ferreira, María Luján. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; ArgentinaResearch Trends2011-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/64696Lassalle, Verónica Leticia; Pirillo, Silvina; Rueda, Elsa Haydee; Ferreira, María Luján; An accurate UV/visible method to quantify proteins and nzymes:Impact of aggregation, buffer concentration and the nature of the standard; Research Trends; Current Topics in Analytical Chemistry; 8; 10-2011; 83-930972-4451CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.researchtrends.net/tia/abstract.asp?in=0&vn=8&tid=30&aid=3333&pub=2011&type=info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:35:23Zoai:ri.conicet.gov.ar:11336/64696instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:35:23.942CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv An accurate UV/visible method to quantify proteins and nzymes:Impact of aggregation, buffer concentration and the nature of the standard
title An accurate UV/visible method to quantify proteins and nzymes:Impact of aggregation, buffer concentration and the nature of the standard
spellingShingle An accurate UV/visible method to quantify proteins and nzymes:Impact of aggregation, buffer concentration and the nature of the standard
Lassalle, Verónica Leticia
Enzymes
Biocatalyst
Adsorption
Proteins
title_short An accurate UV/visible method to quantify proteins and nzymes:Impact of aggregation, buffer concentration and the nature of the standard
title_full An accurate UV/visible method to quantify proteins and nzymes:Impact of aggregation, buffer concentration and the nature of the standard
title_fullStr An accurate UV/visible method to quantify proteins and nzymes:Impact of aggregation, buffer concentration and the nature of the standard
title_full_unstemmed An accurate UV/visible method to quantify proteins and nzymes:Impact of aggregation, buffer concentration and the nature of the standard
title_sort An accurate UV/visible method to quantify proteins and nzymes:Impact of aggregation, buffer concentration and the nature of the standard
dc.creator.none.fl_str_mv Lassalle, Verónica Leticia
Pirillo, Silvina
Rueda, Elsa Haydee
Ferreira, María Luján
author Lassalle, Verónica Leticia
author_facet Lassalle, Verónica Leticia
Pirillo, Silvina
Rueda, Elsa Haydee
Ferreira, María Luján
author_role author
author2 Pirillo, Silvina
Rueda, Elsa Haydee
Ferreira, María Luján
author2_role author
author
author
dc.subject.none.fl_str_mv Enzymes
Biocatalyst
Adsorption
Proteins
topic Enzymes
Biocatalyst
Adsorption
Proteins
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv A simple, fast and low cost UV/visible based method to quantify proteins or enzymes is presented. This method avoids some drawbacks found using conventional techniques. Representative proteins and enzymes such as bovine serum albumin (BSA), insulin (Ins.), Rhizomucor meihei lipase (RML), Candida rugosa lipase (CRL) and horseradish peroxidase (HRP) were assayed as model. Experiments revealed that the aggregation of the enzyme/protein molecules in aqueous solution was the main cause of inaccurate results obtained with the simple UV/visible method. It was determined that aggregation of proteins/ enzymes in aqueous solutions follows a reversible mechanism that could be reverted by simple magnetic stirring treatment. The results achieved within this study warn about common error sources in protein quantification by UV/visible based methods and clearly shows the magnitude of the mistakes that could be achieved if aggregation and other factors are not considered.
Fil: Lassalle, Verónica Leticia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Pirillo, Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Rueda, Elsa Haydee. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Ferreira, María Luján. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Planta Piloto de Ingeniería Química. Universidad Nacional del Sur. Planta Piloto de Ingeniería Química; Argentina
description A simple, fast and low cost UV/visible based method to quantify proteins or enzymes is presented. This method avoids some drawbacks found using conventional techniques. Representative proteins and enzymes such as bovine serum albumin (BSA), insulin (Ins.), Rhizomucor meihei lipase (RML), Candida rugosa lipase (CRL) and horseradish peroxidase (HRP) were assayed as model. Experiments revealed that the aggregation of the enzyme/protein molecules in aqueous solution was the main cause of inaccurate results obtained with the simple UV/visible method. It was determined that aggregation of proteins/ enzymes in aqueous solutions follows a reversible mechanism that could be reverted by simple magnetic stirring treatment. The results achieved within this study warn about common error sources in protein quantification by UV/visible based methods and clearly shows the magnitude of the mistakes that could be achieved if aggregation and other factors are not considered.
publishDate 2011
dc.date.none.fl_str_mv 2011-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/64696
Lassalle, Verónica Leticia; Pirillo, Silvina; Rueda, Elsa Haydee; Ferreira, María Luján; An accurate UV/visible method to quantify proteins and nzymes:Impact of aggregation, buffer concentration and the nature of the standard; Research Trends; Current Topics in Analytical Chemistry; 8; 10-2011; 83-93
0972-4451
CONICET Digital
CONICET
url http://hdl.handle.net/11336/64696
identifier_str_mv Lassalle, Verónica Leticia; Pirillo, Silvina; Rueda, Elsa Haydee; Ferreira, María Luján; An accurate UV/visible method to quantify proteins and nzymes:Impact of aggregation, buffer concentration and the nature of the standard; Research Trends; Current Topics in Analytical Chemistry; 8; 10-2011; 83-93
0972-4451
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.researchtrends.net/tia/abstract.asp?in=0&vn=8&tid=30&aid=3333&pub=2011&type=
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Research Trends
publisher.none.fl_str_mv Research Trends
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613101806157824
score 13.070432

Publicaciones similares