On-chip intermediate potential measurements for the control of O electromigration in multi-channel networks in case of time-dependent potential changes.

Autores
Sydes, Daniel; Kler, Pablo Alejandro; Zipfl, P.; Lutz, D.; Bouwes, Hans; Huhn, Carolin
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
One of the greatest challenges in multi-channel networks for electromigrative separation techniques is the control of the leakage of sample constituents and band broadening at the channel intersections in microfluidic devices or capillary-chip interfaces, which can be achieved using fixed bias or pullback potentials. These may be implemented as the first separation dimension in a 2D setup, where the electric potential at the interface to the second dimension changes with time. Thus, a dynamic control via on-line potential measurement in combination with a feedback system is needed to control electromigration into the side channels. We here present for the first time a prototype for in-channel potential measurements using a low working current in combination with a Si3N4 passivated Ti/Pt electrode at the intersection of the channels in a microfluidic interface. Exemplarily we chose capillary electrophoresis and isotachophoresis as model applications with constant vs. dynamic potential. Parallel on-chip intersection potential measurements were successfully conducted without disturbing capillary zone electrophoretic and isotachophoretic analysis regarding separation and peak performance of amino acids chosen as model analytes. This was possible due to a Si3N4 passivation layer, but also due to an ad-hoc developed high impedance instrumentation, resulting in a very low measuring electric current. Simulations of the detected isotachophoretic cross-section potential allowed a deeper understanding of the potential development during the separation.
Fil: Sydes, Daniel. Iptc - Karls Eberhardt Universität Tübingen; Alemania
Fil: Kler, Pablo Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Centro de Investigaciones en Métodos Computacionales. Universidad Nacional del Litoral. Centro de Investigaciones en Métodos Computacionales; Argentina
Fil: Zipfl, P.. Fachhochschule Aalen; Alemania
Fil: Lutz, D.. Fachhochschule Aalen; Alemania
Fil: Bouwes, Hans. Ix-factory Gmbh; Alemania
Fil: Huhn, Carolin. Iptc - Karls Eberhardt Universität Tübingen; Alemania
Materia
Capacitively Coupled Contactless Conductivity Detection
Capillary Electrophoresis
Dynamic Electric Potential Control
Glass Microfluidic Interface
Isotachophoresis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/78626
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling On-chip intermediate potential measurements for the control of O electromigration in multi-channel networks in case of time-dependent potential changes.Sydes, DanielKler, Pablo AlejandroZipfl, P.Lutz, D.Bouwes, HansHuhn, CarolinCapacitively Coupled Contactless Conductivity DetectionCapillary ElectrophoresisDynamic Electric Potential ControlGlass Microfluidic InterfaceIsotachophoresishttps://purl.org/becyt/ford/2.2https://purl.org/becyt/ford/2One of the greatest challenges in multi-channel networks for electromigrative separation techniques is the control of the leakage of sample constituents and band broadening at the channel intersections in microfluidic devices or capillary-chip interfaces, which can be achieved using fixed bias or pullback potentials. These may be implemented as the first separation dimension in a 2D setup, where the electric potential at the interface to the second dimension changes with time. Thus, a dynamic control via on-line potential measurement in combination with a feedback system is needed to control electromigration into the side channels. We here present for the first time a prototype for in-channel potential measurements using a low working current in combination with a Si3N4 passivated Ti/Pt electrode at the intersection of the channels in a microfluidic interface. Exemplarily we chose capillary electrophoresis and isotachophoresis as model applications with constant vs. dynamic potential. Parallel on-chip intersection potential measurements were successfully conducted without disturbing capillary zone electrophoretic and isotachophoretic analysis regarding separation and peak performance of amino acids chosen as model analytes. This was possible due to a Si3N4 passivation layer, but also due to an ad-hoc developed high impedance instrumentation, resulting in a very low measuring electric current. Simulations of the detected isotachophoretic cross-section potential allowed a deeper understanding of the potential development during the separation.Fil: Sydes, Daniel. Iptc - Karls Eberhardt Universität Tübingen; AlemaniaFil: Kler, Pablo Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Centro de Investigaciones en Métodos Computacionales. Universidad Nacional del Litoral. Centro de Investigaciones en Métodos Computacionales; ArgentinaFil: Zipfl, P.. Fachhochschule Aalen; AlemaniaFil: Lutz, D.. Fachhochschule Aalen; AlemaniaFil: Bouwes, Hans. Ix-factory Gmbh; AlemaniaFil: Huhn, Carolin. Iptc - Karls Eberhardt Universität Tübingen; AlemaniaElsevier Science Sa2017-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/78626Sydes, Daniel; Kler, Pablo Alejandro; Zipfl, P.; Lutz, D.; Bouwes, Hans; et al.; On-chip intermediate potential measurements for the control of O electromigration in multi-channel networks in case of time-dependent potential changes.; Elsevier Science Sa; Sensors and Actuators B: Chemical; 240; 3-2017; 330-3370925-4005CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.snb.2016.08.152info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:00:36Zoai:ri.conicet.gov.ar:11336/78626instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:00:36.438CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv On-chip intermediate potential measurements for the control of O electromigration in multi-channel networks in case of time-dependent potential changes.
title On-chip intermediate potential measurements for the control of O electromigration in multi-channel networks in case of time-dependent potential changes.
spellingShingle On-chip intermediate potential measurements for the control of O electromigration in multi-channel networks in case of time-dependent potential changes.
Sydes, Daniel
Capacitively Coupled Contactless Conductivity Detection
Capillary Electrophoresis
Dynamic Electric Potential Control
Glass Microfluidic Interface
Isotachophoresis
title_short On-chip intermediate potential measurements for the control of O electromigration in multi-channel networks in case of time-dependent potential changes.
title_full On-chip intermediate potential measurements for the control of O electromigration in multi-channel networks in case of time-dependent potential changes.
title_fullStr On-chip intermediate potential measurements for the control of O electromigration in multi-channel networks in case of time-dependent potential changes.
title_full_unstemmed On-chip intermediate potential measurements for the control of O electromigration in multi-channel networks in case of time-dependent potential changes.
title_sort On-chip intermediate potential measurements for the control of O electromigration in multi-channel networks in case of time-dependent potential changes.
dc.creator.none.fl_str_mv Sydes, Daniel
Kler, Pablo Alejandro
Zipfl, P.
Lutz, D.
Bouwes, Hans
Huhn, Carolin
author Sydes, Daniel
author_facet Sydes, Daniel
Kler, Pablo Alejandro
Zipfl, P.
Lutz, D.
Bouwes, Hans
Huhn, Carolin
author_role author
author2 Kler, Pablo Alejandro
Zipfl, P.
Lutz, D.
Bouwes, Hans
Huhn, Carolin
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Capacitively Coupled Contactless Conductivity Detection
Capillary Electrophoresis
Dynamic Electric Potential Control
Glass Microfluidic Interface
Isotachophoresis
topic Capacitively Coupled Contactless Conductivity Detection
Capillary Electrophoresis
Dynamic Electric Potential Control
Glass Microfluidic Interface
Isotachophoresis
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.2
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv One of the greatest challenges in multi-channel networks for electromigrative separation techniques is the control of the leakage of sample constituents and band broadening at the channel intersections in microfluidic devices or capillary-chip interfaces, which can be achieved using fixed bias or pullback potentials. These may be implemented as the first separation dimension in a 2D setup, where the electric potential at the interface to the second dimension changes with time. Thus, a dynamic control via on-line potential measurement in combination with a feedback system is needed to control electromigration into the side channels. We here present for the first time a prototype for in-channel potential measurements using a low working current in combination with a Si3N4 passivated Ti/Pt electrode at the intersection of the channels in a microfluidic interface. Exemplarily we chose capillary electrophoresis and isotachophoresis as model applications with constant vs. dynamic potential. Parallel on-chip intersection potential measurements were successfully conducted without disturbing capillary zone electrophoretic and isotachophoretic analysis regarding separation and peak performance of amino acids chosen as model analytes. This was possible due to a Si3N4 passivation layer, but also due to an ad-hoc developed high impedance instrumentation, resulting in a very low measuring electric current. Simulations of the detected isotachophoretic cross-section potential allowed a deeper understanding of the potential development during the separation.
Fil: Sydes, Daniel. Iptc - Karls Eberhardt Universität Tübingen; Alemania
Fil: Kler, Pablo Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Centro de Investigaciones en Métodos Computacionales. Universidad Nacional del Litoral. Centro de Investigaciones en Métodos Computacionales; Argentina
Fil: Zipfl, P.. Fachhochschule Aalen; Alemania
Fil: Lutz, D.. Fachhochschule Aalen; Alemania
Fil: Bouwes, Hans. Ix-factory Gmbh; Alemania
Fil: Huhn, Carolin. Iptc - Karls Eberhardt Universität Tübingen; Alemania
description One of the greatest challenges in multi-channel networks for electromigrative separation techniques is the control of the leakage of sample constituents and band broadening at the channel intersections in microfluidic devices or capillary-chip interfaces, which can be achieved using fixed bias or pullback potentials. These may be implemented as the first separation dimension in a 2D setup, where the electric potential at the interface to the second dimension changes with time. Thus, a dynamic control via on-line potential measurement in combination with a feedback system is needed to control electromigration into the side channels. We here present for the first time a prototype for in-channel potential measurements using a low working current in combination with a Si3N4 passivated Ti/Pt electrode at the intersection of the channels in a microfluidic interface. Exemplarily we chose capillary electrophoresis and isotachophoresis as model applications with constant vs. dynamic potential. Parallel on-chip intersection potential measurements were successfully conducted without disturbing capillary zone electrophoretic and isotachophoretic analysis regarding separation and peak performance of amino acids chosen as model analytes. This was possible due to a Si3N4 passivation layer, but also due to an ad-hoc developed high impedance instrumentation, resulting in a very low measuring electric current. Simulations of the detected isotachophoretic cross-section potential allowed a deeper understanding of the potential development during the separation.
publishDate 2017
dc.date.none.fl_str_mv 2017-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/78626
Sydes, Daniel; Kler, Pablo Alejandro; Zipfl, P.; Lutz, D.; Bouwes, Hans; et al.; On-chip intermediate potential measurements for the control of O electromigration in multi-channel networks in case of time-dependent potential changes.; Elsevier Science Sa; Sensors and Actuators B: Chemical; 240; 3-2017; 330-337
0925-4005
CONICET Digital
CONICET
url http://hdl.handle.net/11336/78626
identifier_str_mv Sydes, Daniel; Kler, Pablo Alejandro; Zipfl, P.; Lutz, D.; Bouwes, Hans; et al.; On-chip intermediate potential measurements for the control of O electromigration in multi-channel networks in case of time-dependent potential changes.; Elsevier Science Sa; Sensors and Actuators B: Chemical; 240; 3-2017; 330-337
0925-4005
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.snb.2016.08.152
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Sa
publisher.none.fl_str_mv Elsevier Science Sa
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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