On-chip intermediate LED-IF-based detection for the control of electromigration in multichannel networks

Autores
Sydes, Daniel; Kler, Pablo Alejandro; Meyer, Hans; Zipfl, Peter; Lutz, Daniel; Huhn, Carolin
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Monitoring analytes during the transfer step from the first to the second dimension in multidimensional electrophoretic separations is crucial to determine and control the optimal time point for sample transfer and thus to avoid band broadening or unwanted splitting of the sample band with consequent sample loss. A spatially resolved intermediate on-chip LED-induced fluorescence detection system was successfully implemented for a hybrid capillary-chip glass interface. The setup includes a high-power 455-nm LED prototype as an excitation light source and a linear light fiber array consisting of 23 light fibers with a diameter of 100 μm for spatially resolved fluorescence detection in combination with a push-broom imager for hyperspectral detection. Using a basic FITC solution, the linear working range was determined to be 0.125 to 25 μg/ml for a single light guide and the absolute detection limit was 0.04 fmol at a signal-to-noise ratio of 4. With the setup presented here, labeled β-lactoglobulin focused via capillary isoelectric focusing was detectable on-chip with a sufficient intensity to monitor the analyte band transfer in the glass-chip interface demonstrating its applicability for full or intermediate on-chip detection.
Fil: Sydes, Daniel. Eberhard Karls Universität Tübingen; Alemania
Fil: Kler, Pablo Alejandro. Eberhard Karls Universität Tübingen; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Centro de Investigaciones en Métodos Computacionales. Universidad Nacional del Litoral. Centro de Investigaciones en Métodos Computacionales; Argentina
Fil: Meyer, Hans. J&M Analytik AG; Alemania
Fil: Zipfl, Peter. University of Applied Sciences Aalen. Optoelectronics and Laser Technology Department; Alemania
Fil: Lutz, Daniel. CalvaSens GmbH; Alemania
Fil: Huhn, Carolin. Eberhard Karls Universität Tübingen; Alemania
Materia
Glass Microchip
Isoelectric Focusing
On-Chip Led-If Detection
Two-Dimensional Separations
Voltage Switching
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/38288

id CONICETDig_61ce3e2fa25df843e5dc643ff2f23cd6
oai_identifier_str oai:ri.conicet.gov.ar:11336/38288
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling On-chip intermediate LED-IF-based detection for the control of electromigration in multichannel networksSydes, DanielKler, Pablo AlejandroMeyer, HansZipfl, PeterLutz, DanielHuhn, CarolinGlass MicrochipIsoelectric FocusingOn-Chip Led-If DetectionTwo-Dimensional SeparationsVoltage Switchinghttps://purl.org/becyt/ford/2.2https://purl.org/becyt/ford/2Monitoring analytes during the transfer step from the first to the second dimension in multidimensional electrophoretic separations is crucial to determine and control the optimal time point for sample transfer and thus to avoid band broadening or unwanted splitting of the sample band with consequent sample loss. A spatially resolved intermediate on-chip LED-induced fluorescence detection system was successfully implemented for a hybrid capillary-chip glass interface. The setup includes a high-power 455-nm LED prototype as an excitation light source and a linear light fiber array consisting of 23 light fibers with a diameter of 100 μm for spatially resolved fluorescence detection in combination with a push-broom imager for hyperspectral detection. Using a basic FITC solution, the linear working range was determined to be 0.125 to 25 μg/ml for a single light guide and the absolute detection limit was 0.04 fmol at a signal-to-noise ratio of 4. With the setup presented here, labeled β-lactoglobulin focused via capillary isoelectric focusing was detectable on-chip with a sufficient intensity to monitor the analyte band transfer in the glass-chip interface demonstrating its applicability for full or intermediate on-chip detection.Fil: Sydes, Daniel. Eberhard Karls Universität Tübingen; AlemaniaFil: Kler, Pablo Alejandro. Eberhard Karls Universität Tübingen; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Centro de Investigaciones en Métodos Computacionales. Universidad Nacional del Litoral. Centro de Investigaciones en Métodos Computacionales; ArgentinaFil: Meyer, Hans. J&M Analytik AG; AlemaniaFil: Zipfl, Peter. University of Applied Sciences Aalen. Optoelectronics and Laser Technology Department; AlemaniaFil: Lutz, Daniel. CalvaSens GmbH; AlemaniaFil: Huhn, Carolin. Eberhard Karls Universität Tübingen; AlemaniaSpringer Heidelberg2016-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/38288Sydes, Daniel; Kler, Pablo Alejandro; Meyer, Hans; Zipfl, Peter; Lutz, Daniel; et al.; On-chip intermediate LED-IF-based detection for the control of electromigration in multichannel networks; Springer Heidelberg; Analytical and Bioanalytical Chemistry; 408; 30; 12-2016; 8713-87251618-26421618-2650CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007/s00216-016-0033-8info:eu-repo/semantics/altIdentifier/doi/10.1007/s00216-016-0033-8info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:20:53Zoai:ri.conicet.gov.ar:11336/38288instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:20:53.809CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv On-chip intermediate LED-IF-based detection for the control of electromigration in multichannel networks
title On-chip intermediate LED-IF-based detection for the control of electromigration in multichannel networks
spellingShingle On-chip intermediate LED-IF-based detection for the control of electromigration in multichannel networks
Sydes, Daniel
Glass Microchip
Isoelectric Focusing
On-Chip Led-If Detection
Two-Dimensional Separations
Voltage Switching
title_short On-chip intermediate LED-IF-based detection for the control of electromigration in multichannel networks
title_full On-chip intermediate LED-IF-based detection for the control of electromigration in multichannel networks
title_fullStr On-chip intermediate LED-IF-based detection for the control of electromigration in multichannel networks
title_full_unstemmed On-chip intermediate LED-IF-based detection for the control of electromigration in multichannel networks
title_sort On-chip intermediate LED-IF-based detection for the control of electromigration in multichannel networks
dc.creator.none.fl_str_mv Sydes, Daniel
Kler, Pablo Alejandro
Meyer, Hans
Zipfl, Peter
Lutz, Daniel
Huhn, Carolin
author Sydes, Daniel
author_facet Sydes, Daniel
Kler, Pablo Alejandro
Meyer, Hans
Zipfl, Peter
Lutz, Daniel
Huhn, Carolin
author_role author
author2 Kler, Pablo Alejandro
Meyer, Hans
Zipfl, Peter
Lutz, Daniel
Huhn, Carolin
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Glass Microchip
Isoelectric Focusing
On-Chip Led-If Detection
Two-Dimensional Separations
Voltage Switching
topic Glass Microchip
Isoelectric Focusing
On-Chip Led-If Detection
Two-Dimensional Separations
Voltage Switching
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.2
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Monitoring analytes during the transfer step from the first to the second dimension in multidimensional electrophoretic separations is crucial to determine and control the optimal time point for sample transfer and thus to avoid band broadening or unwanted splitting of the sample band with consequent sample loss. A spatially resolved intermediate on-chip LED-induced fluorescence detection system was successfully implemented for a hybrid capillary-chip glass interface. The setup includes a high-power 455-nm LED prototype as an excitation light source and a linear light fiber array consisting of 23 light fibers with a diameter of 100 μm for spatially resolved fluorescence detection in combination with a push-broom imager for hyperspectral detection. Using a basic FITC solution, the linear working range was determined to be 0.125 to 25 μg/ml for a single light guide and the absolute detection limit was 0.04 fmol at a signal-to-noise ratio of 4. With the setup presented here, labeled β-lactoglobulin focused via capillary isoelectric focusing was detectable on-chip with a sufficient intensity to monitor the analyte band transfer in the glass-chip interface demonstrating its applicability for full or intermediate on-chip detection.
Fil: Sydes, Daniel. Eberhard Karls Universität Tübingen; Alemania
Fil: Kler, Pablo Alejandro. Eberhard Karls Universität Tübingen; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Centro de Investigaciones en Métodos Computacionales. Universidad Nacional del Litoral. Centro de Investigaciones en Métodos Computacionales; Argentina
Fil: Meyer, Hans. J&M Analytik AG; Alemania
Fil: Zipfl, Peter. University of Applied Sciences Aalen. Optoelectronics and Laser Technology Department; Alemania
Fil: Lutz, Daniel. CalvaSens GmbH; Alemania
Fil: Huhn, Carolin. Eberhard Karls Universität Tübingen; Alemania
description Monitoring analytes during the transfer step from the first to the second dimension in multidimensional electrophoretic separations is crucial to determine and control the optimal time point for sample transfer and thus to avoid band broadening or unwanted splitting of the sample band with consequent sample loss. A spatially resolved intermediate on-chip LED-induced fluorescence detection system was successfully implemented for a hybrid capillary-chip glass interface. The setup includes a high-power 455-nm LED prototype as an excitation light source and a linear light fiber array consisting of 23 light fibers with a diameter of 100 μm for spatially resolved fluorescence detection in combination with a push-broom imager for hyperspectral detection. Using a basic FITC solution, the linear working range was determined to be 0.125 to 25 μg/ml for a single light guide and the absolute detection limit was 0.04 fmol at a signal-to-noise ratio of 4. With the setup presented here, labeled β-lactoglobulin focused via capillary isoelectric focusing was detectable on-chip with a sufficient intensity to monitor the analyte band transfer in the glass-chip interface demonstrating its applicability for full or intermediate on-chip detection.
publishDate 2016
dc.date.none.fl_str_mv 2016-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/38288
Sydes, Daniel; Kler, Pablo Alejandro; Meyer, Hans; Zipfl, Peter; Lutz, Daniel; et al.; On-chip intermediate LED-IF-based detection for the control of electromigration in multichannel networks; Springer Heidelberg; Analytical and Bioanalytical Chemistry; 408; 30; 12-2016; 8713-8725
1618-2642
1618-2650
CONICET Digital
CONICET
url http://hdl.handle.net/11336/38288
identifier_str_mv Sydes, Daniel; Kler, Pablo Alejandro; Meyer, Hans; Zipfl, Peter; Lutz, Daniel; et al.; On-chip intermediate LED-IF-based detection for the control of electromigration in multichannel networks; Springer Heidelberg; Analytical and Bioanalytical Chemistry; 408; 30; 12-2016; 8713-8725
1618-2642
1618-2650
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007/s00216-016-0033-8
info:eu-repo/semantics/altIdentifier/doi/10.1007/s00216-016-0033-8
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer Heidelberg
publisher.none.fl_str_mv Springer Heidelberg
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614193607606272
score 13.070432