On-chip intermediate LED-IF-based detection for the control of electromigration in multichannel networks
- Autores
- Sydes, Daniel; Kler, Pablo Alejandro; Meyer, Hans; Zipfl, Peter; Lutz, Daniel; Huhn, Carolin
- Año de publicación
- 2016
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Monitoring analytes during the transfer step from the first to the second dimension in multidimensional electrophoretic separations is crucial to determine and control the optimal time point for sample transfer and thus to avoid band broadening or unwanted splitting of the sample band with consequent sample loss. A spatially resolved intermediate on-chip LED-induced fluorescence detection system was successfully implemented for a hybrid capillary-chip glass interface. The setup includes a high-power 455-nm LED prototype as an excitation light source and a linear light fiber array consisting of 23 light fibers with a diameter of 100 μm for spatially resolved fluorescence detection in combination with a push-broom imager for hyperspectral detection. Using a basic FITC solution, the linear working range was determined to be 0.125 to 25 μg/ml for a single light guide and the absolute detection limit was 0.04 fmol at a signal-to-noise ratio of 4. With the setup presented here, labeled β-lactoglobulin focused via capillary isoelectric focusing was detectable on-chip with a sufficient intensity to monitor the analyte band transfer in the glass-chip interface demonstrating its applicability for full or intermediate on-chip detection.
Fil: Sydes, Daniel. Eberhard Karls Universität Tübingen; Alemania
Fil: Kler, Pablo Alejandro. Eberhard Karls Universität Tübingen; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Centro de Investigaciones en Métodos Computacionales. Universidad Nacional del Litoral. Centro de Investigaciones en Métodos Computacionales; Argentina
Fil: Meyer, Hans. J&M Analytik AG; Alemania
Fil: Zipfl, Peter. University of Applied Sciences Aalen. Optoelectronics and Laser Technology Department; Alemania
Fil: Lutz, Daniel. CalvaSens GmbH; Alemania
Fil: Huhn, Carolin. Eberhard Karls Universität Tübingen; Alemania - Materia
-
Glass Microchip
Isoelectric Focusing
On-Chip Led-If Detection
Two-Dimensional Separations
Voltage Switching - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/38288
Ver los metadatos del registro completo
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On-chip intermediate LED-IF-based detection for the control of electromigration in multichannel networksSydes, DanielKler, Pablo AlejandroMeyer, HansZipfl, PeterLutz, DanielHuhn, CarolinGlass MicrochipIsoelectric FocusingOn-Chip Led-If DetectionTwo-Dimensional SeparationsVoltage Switchinghttps://purl.org/becyt/ford/2.2https://purl.org/becyt/ford/2Monitoring analytes during the transfer step from the first to the second dimension in multidimensional electrophoretic separations is crucial to determine and control the optimal time point for sample transfer and thus to avoid band broadening or unwanted splitting of the sample band with consequent sample loss. A spatially resolved intermediate on-chip LED-induced fluorescence detection system was successfully implemented for a hybrid capillary-chip glass interface. The setup includes a high-power 455-nm LED prototype as an excitation light source and a linear light fiber array consisting of 23 light fibers with a diameter of 100 μm for spatially resolved fluorescence detection in combination with a push-broom imager for hyperspectral detection. Using a basic FITC solution, the linear working range was determined to be 0.125 to 25 μg/ml for a single light guide and the absolute detection limit was 0.04 fmol at a signal-to-noise ratio of 4. With the setup presented here, labeled β-lactoglobulin focused via capillary isoelectric focusing was detectable on-chip with a sufficient intensity to monitor the analyte band transfer in the glass-chip interface demonstrating its applicability for full or intermediate on-chip detection.Fil: Sydes, Daniel. Eberhard Karls Universität Tübingen; AlemaniaFil: Kler, Pablo Alejandro. Eberhard Karls Universität Tübingen; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Centro de Investigaciones en Métodos Computacionales. Universidad Nacional del Litoral. Centro de Investigaciones en Métodos Computacionales; ArgentinaFil: Meyer, Hans. J&M Analytik AG; AlemaniaFil: Zipfl, Peter. University of Applied Sciences Aalen. Optoelectronics and Laser Technology Department; AlemaniaFil: Lutz, Daniel. CalvaSens GmbH; AlemaniaFil: Huhn, Carolin. Eberhard Karls Universität Tübingen; AlemaniaSpringer Heidelberg2016-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/38288Sydes, Daniel; Kler, Pablo Alejandro; Meyer, Hans; Zipfl, Peter; Lutz, Daniel; et al.; On-chip intermediate LED-IF-based detection for the control of electromigration in multichannel networks; Springer Heidelberg; Analytical and Bioanalytical Chemistry; 408; 30; 12-2016; 8713-87251618-26421618-2650CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007/s00216-016-0033-8info:eu-repo/semantics/altIdentifier/doi/10.1007/s00216-016-0033-8info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:20:53Zoai:ri.conicet.gov.ar:11336/38288instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:20:53.809CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
On-chip intermediate LED-IF-based detection for the control of electromigration in multichannel networks |
title |
On-chip intermediate LED-IF-based detection for the control of electromigration in multichannel networks |
spellingShingle |
On-chip intermediate LED-IF-based detection for the control of electromigration in multichannel networks Sydes, Daniel Glass Microchip Isoelectric Focusing On-Chip Led-If Detection Two-Dimensional Separations Voltage Switching |
title_short |
On-chip intermediate LED-IF-based detection for the control of electromigration in multichannel networks |
title_full |
On-chip intermediate LED-IF-based detection for the control of electromigration in multichannel networks |
title_fullStr |
On-chip intermediate LED-IF-based detection for the control of electromigration in multichannel networks |
title_full_unstemmed |
On-chip intermediate LED-IF-based detection for the control of electromigration in multichannel networks |
title_sort |
On-chip intermediate LED-IF-based detection for the control of electromigration in multichannel networks |
dc.creator.none.fl_str_mv |
Sydes, Daniel Kler, Pablo Alejandro Meyer, Hans Zipfl, Peter Lutz, Daniel Huhn, Carolin |
author |
Sydes, Daniel |
author_facet |
Sydes, Daniel Kler, Pablo Alejandro Meyer, Hans Zipfl, Peter Lutz, Daniel Huhn, Carolin |
author_role |
author |
author2 |
Kler, Pablo Alejandro Meyer, Hans Zipfl, Peter Lutz, Daniel Huhn, Carolin |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
Glass Microchip Isoelectric Focusing On-Chip Led-If Detection Two-Dimensional Separations Voltage Switching |
topic |
Glass Microchip Isoelectric Focusing On-Chip Led-If Detection Two-Dimensional Separations Voltage Switching |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/2.2 https://purl.org/becyt/ford/2 |
dc.description.none.fl_txt_mv |
Monitoring analytes during the transfer step from the first to the second dimension in multidimensional electrophoretic separations is crucial to determine and control the optimal time point for sample transfer and thus to avoid band broadening or unwanted splitting of the sample band with consequent sample loss. A spatially resolved intermediate on-chip LED-induced fluorescence detection system was successfully implemented for a hybrid capillary-chip glass interface. The setup includes a high-power 455-nm LED prototype as an excitation light source and a linear light fiber array consisting of 23 light fibers with a diameter of 100 μm for spatially resolved fluorescence detection in combination with a push-broom imager for hyperspectral detection. Using a basic FITC solution, the linear working range was determined to be 0.125 to 25 μg/ml for a single light guide and the absolute detection limit was 0.04 fmol at a signal-to-noise ratio of 4. With the setup presented here, labeled β-lactoglobulin focused via capillary isoelectric focusing was detectable on-chip with a sufficient intensity to monitor the analyte band transfer in the glass-chip interface demonstrating its applicability for full or intermediate on-chip detection. Fil: Sydes, Daniel. Eberhard Karls Universität Tübingen; Alemania Fil: Kler, Pablo Alejandro. Eberhard Karls Universität Tübingen; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Centro de Investigaciones en Métodos Computacionales. Universidad Nacional del Litoral. Centro de Investigaciones en Métodos Computacionales; Argentina Fil: Meyer, Hans. J&M Analytik AG; Alemania Fil: Zipfl, Peter. University of Applied Sciences Aalen. Optoelectronics and Laser Technology Department; Alemania Fil: Lutz, Daniel. CalvaSens GmbH; Alemania Fil: Huhn, Carolin. Eberhard Karls Universität Tübingen; Alemania |
description |
Monitoring analytes during the transfer step from the first to the second dimension in multidimensional electrophoretic separations is crucial to determine and control the optimal time point for sample transfer and thus to avoid band broadening or unwanted splitting of the sample band with consequent sample loss. A spatially resolved intermediate on-chip LED-induced fluorescence detection system was successfully implemented for a hybrid capillary-chip glass interface. The setup includes a high-power 455-nm LED prototype as an excitation light source and a linear light fiber array consisting of 23 light fibers with a diameter of 100 μm for spatially resolved fluorescence detection in combination with a push-broom imager for hyperspectral detection. Using a basic FITC solution, the linear working range was determined to be 0.125 to 25 μg/ml for a single light guide and the absolute detection limit was 0.04 fmol at a signal-to-noise ratio of 4. With the setup presented here, labeled β-lactoglobulin focused via capillary isoelectric focusing was detectable on-chip with a sufficient intensity to monitor the analyte band transfer in the glass-chip interface demonstrating its applicability for full or intermediate on-chip detection. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-12 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/38288 Sydes, Daniel; Kler, Pablo Alejandro; Meyer, Hans; Zipfl, Peter; Lutz, Daniel; et al.; On-chip intermediate LED-IF-based detection for the control of electromigration in multichannel networks; Springer Heidelberg; Analytical and Bioanalytical Chemistry; 408; 30; 12-2016; 8713-8725 1618-2642 1618-2650 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/38288 |
identifier_str_mv |
Sydes, Daniel; Kler, Pablo Alejandro; Meyer, Hans; Zipfl, Peter; Lutz, Daniel; et al.; On-chip intermediate LED-IF-based detection for the control of electromigration in multichannel networks; Springer Heidelberg; Analytical and Bioanalytical Chemistry; 408; 30; 12-2016; 8713-8725 1618-2642 1618-2650 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007/s00216-016-0033-8 info:eu-repo/semantics/altIdentifier/doi/10.1007/s00216-016-0033-8 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Springer Heidelberg |
publisher.none.fl_str_mv |
Springer Heidelberg |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1844614193607606272 |
score |
13.070432 |