Characterization of polymeric-mixed micelles applied as pseudo-stationary phase in MEKC
- Autores
- Flor, Sabrina Andrea; Tripodi, Valeria Paula; Chiappetta, Diego Andrés; Iglesias Rando, Matías; Lucangioli, Silvia Edith
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The characterization of a polymeric mixed-micelle system applied as pseudostationary phase in micellar electrokinetic chromatography (MEKC) is presented. This system consists of sodium dodecyl sulfate (SDS), cholic acid and a polymeric tensioactive, Tetronic 1107®. This combination of the three tensioactive agents has been previously applied in a MEKC system to resolve nine steroid hormones in urine samples. In a physicochemical study of a set of test molecules and nine steroid hormones, different parameters have been calculated not only for the polymeric mixed-micelles system but also for SDS and cholic acid as single tensioactive agents and in combination. The parameters presented were micellar phase residence times, tmic, micellar proportion, tprop,mic, CLogP50 and methylene selectivity. A comparison of each parameter in the different micellar systems allowed to demonstrate that the retention and selectivity of each micellar system applied as PSP is related to the hydrophobicity of the test molecules. An exception was found in the steroid groups, where only the polymeric mixed-micelle system showed different tprop,mic values specially for intermediate and high hydrophobic steroids. The order of capability for interaction with the analytes of the studied pseudostationary phases was demonstrated by the CLogP50 values and the comparison of methylene selectivity. Moreover, cloud point and the CMC values for Tetronic were presented to complete the characterization of the polymeric mixed-micelle system. In addition, dynamic light scattering analysis and transmission electron microscopy demonstrated that the combination of SDS, cholic acid and Tetronic constitutes a polymeric mixed-micelle system.
Fil: Flor, Sabrina Andrea. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Recursos Naturales y Ambiente. Cátedra de Química Analítica; Argentina;
Fil: Tripodi, Valeria Paula. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica; Argentina;
Fil: Chiappetta, Diego Andrés. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina;
Fil: Iglesias Rando, Matías. Universidad de Buenos Aires. Facultad de Farmacia y Bioquimica. Departamento de Tecnologia Farmaceutica; Argentina;
Fil: Lucangioli, Silvia Edith. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina; - Materia
-
Hydrophobicity
Mixed-Micelles
Polymers
Pseudostationary Phase
Retention - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/1821
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oai:ri.conicet.gov.ar:11336/1821 |
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repository_id_str |
3498 |
network_name_str |
CONICET Digital (CONICET) |
spelling |
Characterization of polymeric-mixed micelles applied as pseudo-stationary phase in MEKCFlor, Sabrina AndreaTripodi, Valeria PaulaChiappetta, Diego AndrésIglesias Rando, MatíasLucangioli, Silvia EdithHydrophobicityMixed-MicellesPolymersPseudostationary PhaseRetentionhttps://purl.org/becyt/ford/2.4https://purl.org/becyt/ford/2https://purl.org/becyt/ford/2.6https://purl.org/becyt/ford/2The characterization of a polymeric mixed-micelle system applied as pseudostationary phase in micellar electrokinetic chromatography (MEKC) is presented. This system consists of sodium dodecyl sulfate (SDS), cholic acid and a polymeric tensioactive, Tetronic 1107®. This combination of the three tensioactive agents has been previously applied in a MEKC system to resolve nine steroid hormones in urine samples. In a physicochemical study of a set of test molecules and nine steroid hormones, different parameters have been calculated not only for the polymeric mixed-micelles system but also for SDS and cholic acid as single tensioactive agents and in combination. The parameters presented were micellar phase residence times, tmic, micellar proportion, tprop,mic, CLogP50 and methylene selectivity. A comparison of each parameter in the different micellar systems allowed to demonstrate that the retention and selectivity of each micellar system applied as PSP is related to the hydrophobicity of the test molecules. An exception was found in the steroid groups, where only the polymeric mixed-micelle system showed different tprop,mic values specially for intermediate and high hydrophobic steroids. The order of capability for interaction with the analytes of the studied pseudostationary phases was demonstrated by the CLogP50 values and the comparison of methylene selectivity. Moreover, cloud point and the CMC values for Tetronic were presented to complete the characterization of the polymeric mixed-micelle system. In addition, dynamic light scattering analysis and transmission electron microscopy demonstrated that the combination of SDS, cholic acid and Tetronic constitutes a polymeric mixed-micelle system.Fil: Flor, Sabrina Andrea. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Recursos Naturales y Ambiente. Cátedra de Química Analítica; Argentina;Fil: Tripodi, Valeria Paula. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica; Argentina;Fil: Chiappetta, Diego Andrés. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina;Fil: Iglesias Rando, Matías. Universidad de Buenos Aires. Facultad de Farmacia y Bioquimica. Departamento de Tecnologia Farmaceutica; Argentina;Fil: Lucangioli, Silvia Edith. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina;Research Trends2013-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/1821Flor, Sabrina Andrea; Tripodi, Valeria Paula; Chiappetta, Diego Andrés; Iglesias Rando, Matías; Lucangioli, Silvia Edith; Characterization of polymeric-mixed micelles applied as pseudo-stationary phase in MEKC; Research Trends; Trends in Chromatography; 8; 8-2013; 43-540972-8635enginfo:eu-repo/semantics/altIdentifier/url/http://www.researchtrends.net/tia/abstract.asp?in=0&vn=8&tid=60&aid=4794&pub=2013&type=info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:14:37Zoai:ri.conicet.gov.ar:11336/1821instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:14:37.708CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Characterization of polymeric-mixed micelles applied as pseudo-stationary phase in MEKC |
title |
Characterization of polymeric-mixed micelles applied as pseudo-stationary phase in MEKC |
spellingShingle |
Characterization of polymeric-mixed micelles applied as pseudo-stationary phase in MEKC Flor, Sabrina Andrea Hydrophobicity Mixed-Micelles Polymers Pseudostationary Phase Retention |
title_short |
Characterization of polymeric-mixed micelles applied as pseudo-stationary phase in MEKC |
title_full |
Characterization of polymeric-mixed micelles applied as pseudo-stationary phase in MEKC |
title_fullStr |
Characterization of polymeric-mixed micelles applied as pseudo-stationary phase in MEKC |
title_full_unstemmed |
Characterization of polymeric-mixed micelles applied as pseudo-stationary phase in MEKC |
title_sort |
Characterization of polymeric-mixed micelles applied as pseudo-stationary phase in MEKC |
dc.creator.none.fl_str_mv |
Flor, Sabrina Andrea Tripodi, Valeria Paula Chiappetta, Diego Andrés Iglesias Rando, Matías Lucangioli, Silvia Edith |
author |
Flor, Sabrina Andrea |
author_facet |
Flor, Sabrina Andrea Tripodi, Valeria Paula Chiappetta, Diego Andrés Iglesias Rando, Matías Lucangioli, Silvia Edith |
author_role |
author |
author2 |
Tripodi, Valeria Paula Chiappetta, Diego Andrés Iglesias Rando, Matías Lucangioli, Silvia Edith |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
Hydrophobicity Mixed-Micelles Polymers Pseudostationary Phase Retention |
topic |
Hydrophobicity Mixed-Micelles Polymers Pseudostationary Phase Retention |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/2.4 https://purl.org/becyt/ford/2 https://purl.org/becyt/ford/2.6 https://purl.org/becyt/ford/2 |
dc.description.none.fl_txt_mv |
The characterization of a polymeric mixed-micelle system applied as pseudostationary phase in micellar electrokinetic chromatography (MEKC) is presented. This system consists of sodium dodecyl sulfate (SDS), cholic acid and a polymeric tensioactive, Tetronic 1107®. This combination of the three tensioactive agents has been previously applied in a MEKC system to resolve nine steroid hormones in urine samples. In a physicochemical study of a set of test molecules and nine steroid hormones, different parameters have been calculated not only for the polymeric mixed-micelles system but also for SDS and cholic acid as single tensioactive agents and in combination. The parameters presented were micellar phase residence times, tmic, micellar proportion, tprop,mic, CLogP50 and methylene selectivity. A comparison of each parameter in the different micellar systems allowed to demonstrate that the retention and selectivity of each micellar system applied as PSP is related to the hydrophobicity of the test molecules. An exception was found in the steroid groups, where only the polymeric mixed-micelle system showed different tprop,mic values specially for intermediate and high hydrophobic steroids. The order of capability for interaction with the analytes of the studied pseudostationary phases was demonstrated by the CLogP50 values and the comparison of methylene selectivity. Moreover, cloud point and the CMC values for Tetronic were presented to complete the characterization of the polymeric mixed-micelle system. In addition, dynamic light scattering analysis and transmission electron microscopy demonstrated that the combination of SDS, cholic acid and Tetronic constitutes a polymeric mixed-micelle system. Fil: Flor, Sabrina Andrea. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Recursos Naturales y Ambiente. Cátedra de Química Analítica; Argentina; Fil: Tripodi, Valeria Paula. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica; Argentina; Fil: Chiappetta, Diego Andrés. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina; Fil: Iglesias Rando, Matías. Universidad de Buenos Aires. Facultad de Farmacia y Bioquimica. Departamento de Tecnologia Farmaceutica; Argentina; Fil: Lucangioli, Silvia Edith. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina; |
description |
The characterization of a polymeric mixed-micelle system applied as pseudostationary phase in micellar electrokinetic chromatography (MEKC) is presented. This system consists of sodium dodecyl sulfate (SDS), cholic acid and a polymeric tensioactive, Tetronic 1107®. This combination of the three tensioactive agents has been previously applied in a MEKC system to resolve nine steroid hormones in urine samples. In a physicochemical study of a set of test molecules and nine steroid hormones, different parameters have been calculated not only for the polymeric mixed-micelles system but also for SDS and cholic acid as single tensioactive agents and in combination. The parameters presented were micellar phase residence times, tmic, micellar proportion, tprop,mic, CLogP50 and methylene selectivity. A comparison of each parameter in the different micellar systems allowed to demonstrate that the retention and selectivity of each micellar system applied as PSP is related to the hydrophobicity of the test molecules. An exception was found in the steroid groups, where only the polymeric mixed-micelle system showed different tprop,mic values specially for intermediate and high hydrophobic steroids. The order of capability for interaction with the analytes of the studied pseudostationary phases was demonstrated by the CLogP50 values and the comparison of methylene selectivity. Moreover, cloud point and the CMC values for Tetronic were presented to complete the characterization of the polymeric mixed-micelle system. In addition, dynamic light scattering analysis and transmission electron microscopy demonstrated that the combination of SDS, cholic acid and Tetronic constitutes a polymeric mixed-micelle system. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-08 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/1821 Flor, Sabrina Andrea; Tripodi, Valeria Paula; Chiappetta, Diego Andrés; Iglesias Rando, Matías; Lucangioli, Silvia Edith; Characterization of polymeric-mixed micelles applied as pseudo-stationary phase in MEKC; Research Trends; Trends in Chromatography; 8; 8-2013; 43-54 0972-8635 |
url |
http://hdl.handle.net/11336/1821 |
identifier_str_mv |
Flor, Sabrina Andrea; Tripodi, Valeria Paula; Chiappetta, Diego Andrés; Iglesias Rando, Matías; Lucangioli, Silvia Edith; Characterization of polymeric-mixed micelles applied as pseudo-stationary phase in MEKC; Research Trends; Trends in Chromatography; 8; 8-2013; 43-54 0972-8635 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://www.researchtrends.net/tia/abstract.asp?in=0&vn=8&tid=60&aid=4794&pub=2013&type= |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Research Trends |
publisher.none.fl_str_mv |
Research Trends |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844614075405828096 |
score |
13.070432 |