Characterization of polymeric-mixed micelles applied as pseudo-stationary phase in MEKC

Autores
Flor, Sabrina Andrea; Tripodi, Valeria Paula; Chiappetta, Diego Andrés; Iglesias Rando, Matías; Lucangioli, Silvia Edith
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The characterization of a polymeric mixed-micelle system applied as pseudostationary phase in micellar electrokinetic chromatography (MEKC) is presented. This system consists of sodium dodecyl sulfate (SDS), cholic acid and a polymeric tensioactive, Tetronic 1107®. This combination of the three tensioactive agents has been previously applied in a MEKC system to resolve nine steroid hormones in urine samples. In a physicochemical study of a set of test molecules and nine steroid hormones, different parameters have been calculated not only for the polymeric mixed-micelles system but also for SDS and cholic acid as single tensioactive agents and in combination. The parameters presented were micellar phase residence times, tmic, micellar proportion, tprop,mic, CLogP50 and methylene selectivity. A comparison of each parameter in the different micellar systems allowed to demonstrate that the retention and selectivity of each micellar system applied as PSP is related to the hydrophobicity of the test molecules. An exception was found in the steroid groups, where only the polymeric mixed-micelle system showed different tprop,mic values specially for intermediate and high hydrophobic steroids. The order of capability for interaction with the analytes of the studied pseudostationary phases was demonstrated by the CLogP50 values and the comparison of methylene selectivity. Moreover, cloud point and the CMC values for Tetronic were presented to complete the characterization of the polymeric mixed-micelle system. In addition, dynamic light scattering analysis and transmission electron microscopy demonstrated that the combination of SDS, cholic acid and Tetronic constitutes a polymeric mixed-micelle system.
Fil: Flor, Sabrina Andrea. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Recursos Naturales y Ambiente. Cátedra de Química Analítica; Argentina;
Fil: Tripodi, Valeria Paula. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica; Argentina;
Fil: Chiappetta, Diego Andrés. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina;
Fil: Iglesias Rando, Matías. Universidad de Buenos Aires. Facultad de Farmacia y Bioquimica. Departamento de Tecnologia Farmaceutica; Argentina;
Fil: Lucangioli, Silvia Edith. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina;
Materia
Hydrophobicity
Mixed-Micelles
Polymers
Pseudostationary Phase
Retention
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/1821

id CONICETDig_a05049a169c97832614ac70823aaff2c
oai_identifier_str oai:ri.conicet.gov.ar:11336/1821
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Characterization of polymeric-mixed micelles applied as pseudo-stationary phase in MEKCFlor, Sabrina AndreaTripodi, Valeria PaulaChiappetta, Diego AndrésIglesias Rando, MatíasLucangioli, Silvia EdithHydrophobicityMixed-MicellesPolymersPseudostationary PhaseRetentionhttps://purl.org/becyt/ford/2.4https://purl.org/becyt/ford/2https://purl.org/becyt/ford/2.6https://purl.org/becyt/ford/2The characterization of a polymeric mixed-micelle system applied as pseudostationary phase in micellar electrokinetic chromatography (MEKC) is presented. This system consists of sodium dodecyl sulfate (SDS), cholic acid and a polymeric tensioactive, Tetronic 1107®. This combination of the three tensioactive agents has been previously applied in a MEKC system to resolve nine steroid hormones in urine samples. In a physicochemical study of a set of test molecules and nine steroid hormones, different parameters have been calculated not only for the polymeric mixed-micelles system but also for SDS and cholic acid as single tensioactive agents and in combination. The parameters presented were micellar phase residence times, tmic, micellar proportion, tprop,mic, CLogP50 and methylene selectivity. A comparison of each parameter in the different micellar systems allowed to demonstrate that the retention and selectivity of each micellar system applied as PSP is related to the hydrophobicity of the test molecules. An exception was found in the steroid groups, where only the polymeric mixed-micelle system showed different tprop,mic values specially for intermediate and high hydrophobic steroids. The order of capability for interaction with the analytes of the studied pseudostationary phases was demonstrated by the CLogP50 values and the comparison of methylene selectivity. Moreover, cloud point and the CMC values for Tetronic were presented to complete the characterization of the polymeric mixed-micelle system. In addition, dynamic light scattering analysis and transmission electron microscopy demonstrated that the combination of SDS, cholic acid and Tetronic constitutes a polymeric mixed-micelle system.Fil: Flor, Sabrina Andrea. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Recursos Naturales y Ambiente. Cátedra de Química Analítica; Argentina;Fil: Tripodi, Valeria Paula. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica; Argentina;Fil: Chiappetta, Diego Andrés. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina;Fil: Iglesias Rando, Matías. Universidad de Buenos Aires. Facultad de Farmacia y Bioquimica. Departamento de Tecnologia Farmaceutica; Argentina;Fil: Lucangioli, Silvia Edith. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina;Research Trends2013-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/1821Flor, Sabrina Andrea; Tripodi, Valeria Paula; Chiappetta, Diego Andrés; Iglesias Rando, Matías; Lucangioli, Silvia Edith; Characterization of polymeric-mixed micelles applied as pseudo-stationary phase in MEKC; Research Trends; Trends in Chromatography; 8; 8-2013; 43-540972-8635enginfo:eu-repo/semantics/altIdentifier/url/http://www.researchtrends.net/tia/abstract.asp?in=0&vn=8&tid=60&aid=4794&pub=2013&type=info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:14:37Zoai:ri.conicet.gov.ar:11336/1821instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:14:37.708CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Characterization of polymeric-mixed micelles applied as pseudo-stationary phase in MEKC
title Characterization of polymeric-mixed micelles applied as pseudo-stationary phase in MEKC
spellingShingle Characterization of polymeric-mixed micelles applied as pseudo-stationary phase in MEKC
Flor, Sabrina Andrea
Hydrophobicity
Mixed-Micelles
Polymers
Pseudostationary Phase
Retention
title_short Characterization of polymeric-mixed micelles applied as pseudo-stationary phase in MEKC
title_full Characterization of polymeric-mixed micelles applied as pseudo-stationary phase in MEKC
title_fullStr Characterization of polymeric-mixed micelles applied as pseudo-stationary phase in MEKC
title_full_unstemmed Characterization of polymeric-mixed micelles applied as pseudo-stationary phase in MEKC
title_sort Characterization of polymeric-mixed micelles applied as pseudo-stationary phase in MEKC
dc.creator.none.fl_str_mv Flor, Sabrina Andrea
Tripodi, Valeria Paula
Chiappetta, Diego Andrés
Iglesias Rando, Matías
Lucangioli, Silvia Edith
author Flor, Sabrina Andrea
author_facet Flor, Sabrina Andrea
Tripodi, Valeria Paula
Chiappetta, Diego Andrés
Iglesias Rando, Matías
Lucangioli, Silvia Edith
author_role author
author2 Tripodi, Valeria Paula
Chiappetta, Diego Andrés
Iglesias Rando, Matías
Lucangioli, Silvia Edith
author2_role author
author
author
author
dc.subject.none.fl_str_mv Hydrophobicity
Mixed-Micelles
Polymers
Pseudostationary Phase
Retention
topic Hydrophobicity
Mixed-Micelles
Polymers
Pseudostationary Phase
Retention
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.4
https://purl.org/becyt/ford/2
https://purl.org/becyt/ford/2.6
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The characterization of a polymeric mixed-micelle system applied as pseudostationary phase in micellar electrokinetic chromatography (MEKC) is presented. This system consists of sodium dodecyl sulfate (SDS), cholic acid and a polymeric tensioactive, Tetronic 1107®. This combination of the three tensioactive agents has been previously applied in a MEKC system to resolve nine steroid hormones in urine samples. In a physicochemical study of a set of test molecules and nine steroid hormones, different parameters have been calculated not only for the polymeric mixed-micelles system but also for SDS and cholic acid as single tensioactive agents and in combination. The parameters presented were micellar phase residence times, tmic, micellar proportion, tprop,mic, CLogP50 and methylene selectivity. A comparison of each parameter in the different micellar systems allowed to demonstrate that the retention and selectivity of each micellar system applied as PSP is related to the hydrophobicity of the test molecules. An exception was found in the steroid groups, where only the polymeric mixed-micelle system showed different tprop,mic values specially for intermediate and high hydrophobic steroids. The order of capability for interaction with the analytes of the studied pseudostationary phases was demonstrated by the CLogP50 values and the comparison of methylene selectivity. Moreover, cloud point and the CMC values for Tetronic were presented to complete the characterization of the polymeric mixed-micelle system. In addition, dynamic light scattering analysis and transmission electron microscopy demonstrated that the combination of SDS, cholic acid and Tetronic constitutes a polymeric mixed-micelle system.
Fil: Flor, Sabrina Andrea. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Recursos Naturales y Ambiente. Cátedra de Química Analítica; Argentina;
Fil: Tripodi, Valeria Paula. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica; Argentina;
Fil: Chiappetta, Diego Andrés. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina;
Fil: Iglesias Rando, Matías. Universidad de Buenos Aires. Facultad de Farmacia y Bioquimica. Departamento de Tecnologia Farmaceutica; Argentina;
Fil: Lucangioli, Silvia Edith. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina;
description The characterization of a polymeric mixed-micelle system applied as pseudostationary phase in micellar electrokinetic chromatography (MEKC) is presented. This system consists of sodium dodecyl sulfate (SDS), cholic acid and a polymeric tensioactive, Tetronic 1107®. This combination of the three tensioactive agents has been previously applied in a MEKC system to resolve nine steroid hormones in urine samples. In a physicochemical study of a set of test molecules and nine steroid hormones, different parameters have been calculated not only for the polymeric mixed-micelles system but also for SDS and cholic acid as single tensioactive agents and in combination. The parameters presented were micellar phase residence times, tmic, micellar proportion, tprop,mic, CLogP50 and methylene selectivity. A comparison of each parameter in the different micellar systems allowed to demonstrate that the retention and selectivity of each micellar system applied as PSP is related to the hydrophobicity of the test molecules. An exception was found in the steroid groups, where only the polymeric mixed-micelle system showed different tprop,mic values specially for intermediate and high hydrophobic steroids. The order of capability for interaction with the analytes of the studied pseudostationary phases was demonstrated by the CLogP50 values and the comparison of methylene selectivity. Moreover, cloud point and the CMC values for Tetronic were presented to complete the characterization of the polymeric mixed-micelle system. In addition, dynamic light scattering analysis and transmission electron microscopy demonstrated that the combination of SDS, cholic acid and Tetronic constitutes a polymeric mixed-micelle system.
publishDate 2013
dc.date.none.fl_str_mv 2013-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/1821
Flor, Sabrina Andrea; Tripodi, Valeria Paula; Chiappetta, Diego Andrés; Iglesias Rando, Matías; Lucangioli, Silvia Edith; Characterization of polymeric-mixed micelles applied as pseudo-stationary phase in MEKC; Research Trends; Trends in Chromatography; 8; 8-2013; 43-54
0972-8635
url http://hdl.handle.net/11336/1821
identifier_str_mv Flor, Sabrina Andrea; Tripodi, Valeria Paula; Chiappetta, Diego Andrés; Iglesias Rando, Matías; Lucangioli, Silvia Edith; Characterization of polymeric-mixed micelles applied as pseudo-stationary phase in MEKC; Research Trends; Trends in Chromatography; 8; 8-2013; 43-54
0972-8635
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.researchtrends.net/tia/abstract.asp?in=0&vn=8&tid=60&aid=4794&pub=2013&type=
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Research Trends
publisher.none.fl_str_mv Research Trends
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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