Studying the interaction between peptides and polymeric nanoparticles used as pseudostationary phase in capillary electrochromatography

Autores
Grela, Denise Agata; Zannoni, Valeria; Vizioli, Nora Matilde
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In this work, the interaction between synthetic bioactive peptides and polymeric nanoparticles (NPs) used as pseudostationary phase in capillary electrochromatography was studied. NPs were prepared from methacrylic acid and ethylenglycol dimethacrylate with benzoyl peroxide by utilizing a precipitation polymerization technique. The reaction was monitored by infrared spectroscopy. Polymer characterization was performed by dynamic light scattering and transmission electron microscopy. Experimental running conditions were tested, including organic solvent proportion in the background electrolyte, capillary conditioning, applied voltage, sample introduction amount, and how NPs were incorporated into the system. A continuous full filling technique in which the NPs were suspended in the entire electrolyte volume as well as a conventional partial filling technique were used. Results obtained at pH 7.0 suggest that the NPs have a very strong interaction with more basic peptides. The interaction between analytes and NPs was found to be predominantly ionic.
Fil: Grela, Denise Agata. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica; Argentina
Fil: Zannoni, Valeria. Instituto Nacional de Tecnología Industrial; Argentina
Fil: Vizioli, Nora Matilde. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica; Argentina
Materia
Capillary Electrochromatography
Capillary Electrophoresis
Peptides
Pseudostationary Phases
Particles
Interaction
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/47292
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/47292
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Studying the interaction between peptides and polymeric nanoparticles used as pseudostationary phase in capillary electrochromatographyGrela, Denise AgataZannoni, ValeriaVizioli, Nora MatildeCapillary ElectrochromatographyCapillary ElectrophoresisPeptidesPseudostationary PhasesParticlesInteractionhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1In this work, the interaction between synthetic bioactive peptides and polymeric nanoparticles (NPs) used as pseudostationary phase in capillary electrochromatography was studied. NPs were prepared from methacrylic acid and ethylenglycol dimethacrylate with benzoyl peroxide by utilizing a precipitation polymerization technique. The reaction was monitored by infrared spectroscopy. Polymer characterization was performed by dynamic light scattering and transmission electron microscopy. Experimental running conditions were tested, including organic solvent proportion in the background electrolyte, capillary conditioning, applied voltage, sample introduction amount, and how NPs were incorporated into the system. A continuous full filling technique in which the NPs were suspended in the entire electrolyte volume as well as a conventional partial filling technique were used. Results obtained at pH 7.0 suggest that the NPs have a very strong interaction with more basic peptides. The interaction between analytes and NPs was found to be predominantly ionic.Fil: Grela, Denise Agata. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica; ArgentinaFil: Zannoni, Valeria. Instituto Nacional de Tecnología Industrial; ArgentinaFil: Vizioli, Nora Matilde. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica; ArgentinaElsevier Science2017-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/47292Grela, Denise Agata; Zannoni, Valeria; Vizioli, Nora Matilde; Studying the interaction between peptides and polymeric nanoparticles used as pseudostationary phase in capillary electrochromatography; Elsevier Science; Microchemical Journal; 130; 1-2017; 153-1560026-265XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.microc.2016.08.015info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0026265X16303332info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:47:06Zoai:ri.conicet.gov.ar:11336/47292instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:47:06.978CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Studying the interaction between peptides and polymeric nanoparticles used as pseudostationary phase in capillary electrochromatography
title Studying the interaction between peptides and polymeric nanoparticles used as pseudostationary phase in capillary electrochromatography
spellingShingle Studying the interaction between peptides and polymeric nanoparticles used as pseudostationary phase in capillary electrochromatography
Grela, Denise Agata
Capillary Electrochromatography
Capillary Electrophoresis
Peptides
Pseudostationary Phases
Particles
Interaction
title_short Studying the interaction between peptides and polymeric nanoparticles used as pseudostationary phase in capillary electrochromatography
title_full Studying the interaction between peptides and polymeric nanoparticles used as pseudostationary phase in capillary electrochromatography
title_fullStr Studying the interaction between peptides and polymeric nanoparticles used as pseudostationary phase in capillary electrochromatography
title_full_unstemmed Studying the interaction between peptides and polymeric nanoparticles used as pseudostationary phase in capillary electrochromatography
title_sort Studying the interaction between peptides and polymeric nanoparticles used as pseudostationary phase in capillary electrochromatography
dc.creator.none.fl_str_mv Grela, Denise Agata
Zannoni, Valeria
Vizioli, Nora Matilde
author Grela, Denise Agata
author_facet Grela, Denise Agata
Zannoni, Valeria
Vizioli, Nora Matilde
author_role author
author2 Zannoni, Valeria
Vizioli, Nora Matilde
author2_role author
author
dc.subject.none.fl_str_mv Capillary Electrochromatography
Capillary Electrophoresis
Peptides
Pseudostationary Phases
Particles
Interaction
topic Capillary Electrochromatography
Capillary Electrophoresis
Peptides
Pseudostationary Phases
Particles
Interaction
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In this work, the interaction between synthetic bioactive peptides and polymeric nanoparticles (NPs) used as pseudostationary phase in capillary electrochromatography was studied. NPs were prepared from methacrylic acid and ethylenglycol dimethacrylate with benzoyl peroxide by utilizing a precipitation polymerization technique. The reaction was monitored by infrared spectroscopy. Polymer characterization was performed by dynamic light scattering and transmission electron microscopy. Experimental running conditions were tested, including organic solvent proportion in the background electrolyte, capillary conditioning, applied voltage, sample introduction amount, and how NPs were incorporated into the system. A continuous full filling technique in which the NPs were suspended in the entire electrolyte volume as well as a conventional partial filling technique were used. Results obtained at pH 7.0 suggest that the NPs have a very strong interaction with more basic peptides. The interaction between analytes and NPs was found to be predominantly ionic.
Fil: Grela, Denise Agata. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica; Argentina
Fil: Zannoni, Valeria. Instituto Nacional de Tecnología Industrial; Argentina
Fil: Vizioli, Nora Matilde. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica; Argentina
description In this work, the interaction between synthetic bioactive peptides and polymeric nanoparticles (NPs) used as pseudostationary phase in capillary electrochromatography was studied. NPs were prepared from methacrylic acid and ethylenglycol dimethacrylate with benzoyl peroxide by utilizing a precipitation polymerization technique. The reaction was monitored by infrared spectroscopy. Polymer characterization was performed by dynamic light scattering and transmission electron microscopy. Experimental running conditions were tested, including organic solvent proportion in the background electrolyte, capillary conditioning, applied voltage, sample introduction amount, and how NPs were incorporated into the system. A continuous full filling technique in which the NPs were suspended in the entire electrolyte volume as well as a conventional partial filling technique were used. Results obtained at pH 7.0 suggest that the NPs have a very strong interaction with more basic peptides. The interaction between analytes and NPs was found to be predominantly ionic.
publishDate 2017
dc.date.none.fl_str_mv 2017-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/47292
Grela, Denise Agata; Zannoni, Valeria; Vizioli, Nora Matilde; Studying the interaction between peptides and polymeric nanoparticles used as pseudostationary phase in capillary electrochromatography; Elsevier Science; Microchemical Journal; 130; 1-2017; 153-156
0026-265X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/47292
identifier_str_mv Grela, Denise Agata; Zannoni, Valeria; Vizioli, Nora Matilde; Studying the interaction between peptides and polymeric nanoparticles used as pseudostationary phase in capillary electrochromatography; Elsevier Science; Microchemical Journal; 130; 1-2017; 153-156
0026-265X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.microc.2016.08.015
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0026265X16303332
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268837764923392
score 13.13397

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