Ghrelin Gene Deletion Alters Pulsatile Growth Hormone Secretion in Adult Female Mice

Autores
Hassouna, Rim; Fernandez, Gimena; Lebrun, Nicolas; Fiquet, Oriane; Roelfsema, Ferdinand; Labarthe, Alexandra; Zizzari, Philippe; Tomasetto, Catherine; Epelbaum, Jacques; Viltart, Odile; Chauveau, Christophe; Perello, Mario; Tolle, Virginie
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Using preproghrelin-deficient mice (Ghrl-/-), we previously observed that preproghrelin modulates pulsatile growth hormone (GH) secretion in post-pubertal male mice. However, the role of ghrelin and its derived peptides in the regulation of growth parameters or feeding in females is unknown. We measured pulsatile GH secretion, growth, metabolic parameters and feeding behavior in adult Ghrl-/- and Ghrl+/+ male and female mice. We also assessed GH release from pituitary explants and hypothalamic growth hormonereleasing hormone (GHRH) expression and immunoreactivity. Body weight and body fat mass, linear growth, spontaneous food intake and food intake following a 48-h fast, GH pituitary contents and GH release from pituitary explants ex vivo, fasting glucose and glucose tolerance were not different among adult Ghrl-/- and Ghrl+/+ male or female mice. In vivo, pulsatile GH secretion was decreased, while approximate entropy, that quantified orderliness of secretion, was increased in adult Ghrl-/- females only, defining more irregular GH pattern. The number of neurons immunoreactive for GHRH visualized in the hypothalamic arcuate nucleus was increased in adult Ghrl-/- females, as compared to Ghrl+/+ females, whereas the expression of GHRH was not different amongst groups. Thus, these results point to sex-specific effects of preproghrelin gene deletion on pulsatile GH secretion, but not feeding, growth or metabolic parameters, in adult mice.
Fil: Hassouna, Rim. Université Paris Cité; Francia. Universite de Bordeaux; Francia. Inserm; Francia
Fil: Fernandez, Gimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; Argentina
Fil: Lebrun, Nicolas. Université Paris Cité; Francia
Fil: Fiquet, Oriane. Universite de Bordeaux; Francia. Université Paris Cité; Francia. Inserm; Francia
Fil: Roelfsema, Ferdinand. Leiden University Medical Center; Países Bajos
Fil: Labarthe, Alexandra. Université Paris Cité; Francia. Universite de Bordeaux; Francia. Inserm; Francia
Fil: Zizzari, Philippe. Universite de Bordeaux; Francia. Université Paris Cité; Francia. Inserm; Francia
Fil: Tomasetto, Catherine. Universite de Bordeaux; Francia. Université Paris Cité; Francia. Inserm; Francia
Fil: Epelbaum, Jacques. Universite de Bordeaux; Francia. Université Paris Cité; Francia. Inserm; Francia
Fil: Viltart, Odile. Universite de Bordeaux; Francia. Université Paris Cité; Francia. Inserm; Francia
Fil: Chauveau, Christophe. Université Du Littoral Côte D‘opale; Francia
Fil: Perello, Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; Argentina
Fil: Tolle, Virginie. Université Paris Cité; Francia
Materia
FOOD INTAKE
GHRELIN
GROWTH HORMONE
PULSATILITY
SEXUAL DIMORPHISM
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/181352
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/181352
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Ghrelin Gene Deletion Alters Pulsatile Growth Hormone Secretion in Adult Female MiceHassouna, RimFernandez, GimenaLebrun, NicolasFiquet, OrianeRoelfsema, FerdinandLabarthe, AlexandraZizzari, PhilippeTomasetto, CatherineEpelbaum, JacquesViltart, OdileChauveau, ChristophePerello, MarioTolle, VirginieFOOD INTAKEGHRELINGROWTH HORMONEPULSATILITYSEXUAL DIMORPHISMhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Using preproghrelin-deficient mice (Ghrl-/-), we previously observed that preproghrelin modulates pulsatile growth hormone (GH) secretion in post-pubertal male mice. However, the role of ghrelin and its derived peptides in the regulation of growth parameters or feeding in females is unknown. We measured pulsatile GH secretion, growth, metabolic parameters and feeding behavior in adult Ghrl-/- and Ghrl+/+ male and female mice. We also assessed GH release from pituitary explants and hypothalamic growth hormonereleasing hormone (GHRH) expression and immunoreactivity. Body weight and body fat mass, linear growth, spontaneous food intake and food intake following a 48-h fast, GH pituitary contents and GH release from pituitary explants ex vivo, fasting glucose and glucose tolerance were not different among adult Ghrl-/- and Ghrl+/+ male or female mice. In vivo, pulsatile GH secretion was decreased, while approximate entropy, that quantified orderliness of secretion, was increased in adult Ghrl-/- females only, defining more irregular GH pattern. The number of neurons immunoreactive for GHRH visualized in the hypothalamic arcuate nucleus was increased in adult Ghrl-/- females, as compared to Ghrl+/+ females, whereas the expression of GHRH was not different amongst groups. Thus, these results point to sex-specific effects of preproghrelin gene deletion on pulsatile GH secretion, but not feeding, growth or metabolic parameters, in adult mice.Fil: Hassouna, Rim. Université Paris Cité; Francia. Universite de Bordeaux; Francia. Inserm; FranciaFil: Fernandez, Gimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; ArgentinaFil: Lebrun, Nicolas. Université Paris Cité; FranciaFil: Fiquet, Oriane. Universite de Bordeaux; Francia. Université Paris Cité; Francia. Inserm; FranciaFil: Roelfsema, Ferdinand. Leiden University Medical Center; Países BajosFil: Labarthe, Alexandra. Université Paris Cité; Francia. Universite de Bordeaux; Francia. Inserm; FranciaFil: Zizzari, Philippe. Universite de Bordeaux; Francia. Université Paris Cité; Francia. Inserm; FranciaFil: Tomasetto, Catherine. Universite de Bordeaux; Francia. Université Paris Cité; Francia. Inserm; FranciaFil: Epelbaum, Jacques. Universite de Bordeaux; Francia. Université Paris Cité; Francia. Inserm; FranciaFil: Viltart, Odile. Universite de Bordeaux; Francia. Université Paris Cité; Francia. Inserm; FranciaFil: Chauveau, Christophe. Université Du Littoral Côte D‘opale; FranciaFil: Perello, Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; ArgentinaFil: Tolle, Virginie. Université Paris Cité; FranciaFrontiers Media2021-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/181352Hassouna, Rim; Fernandez, Gimena; Lebrun, Nicolas; Fiquet, Oriane; Roelfsema, Ferdinand; et al.; Ghrelin Gene Deletion Alters Pulsatile Growth Hormone Secretion in Adult Female Mice; Frontiers Media; Frontiers in Endocrinology; 12; 754522; 10-2021; 1-111664-2392CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fendo.2021.754522/fullinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fendo.2021.754522info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:31:53Zoai:ri.conicet.gov.ar:11336/181352instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:31:53.731CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Ghrelin Gene Deletion Alters Pulsatile Growth Hormone Secretion in Adult Female Mice
title Ghrelin Gene Deletion Alters Pulsatile Growth Hormone Secretion in Adult Female Mice
spellingShingle Ghrelin Gene Deletion Alters Pulsatile Growth Hormone Secretion in Adult Female Mice
Hassouna, Rim
FOOD INTAKE
GHRELIN
GROWTH HORMONE
PULSATILITY
SEXUAL DIMORPHISM
title_short Ghrelin Gene Deletion Alters Pulsatile Growth Hormone Secretion in Adult Female Mice
title_full Ghrelin Gene Deletion Alters Pulsatile Growth Hormone Secretion in Adult Female Mice
title_fullStr Ghrelin Gene Deletion Alters Pulsatile Growth Hormone Secretion in Adult Female Mice
title_full_unstemmed Ghrelin Gene Deletion Alters Pulsatile Growth Hormone Secretion in Adult Female Mice
title_sort Ghrelin Gene Deletion Alters Pulsatile Growth Hormone Secretion in Adult Female Mice
dc.creator.none.fl_str_mv Hassouna, Rim
Fernandez, Gimena
Lebrun, Nicolas
Fiquet, Oriane
Roelfsema, Ferdinand
Labarthe, Alexandra
Zizzari, Philippe
Tomasetto, Catherine
Epelbaum, Jacques
Viltart, Odile
Chauveau, Christophe
Perello, Mario
Tolle, Virginie
author Hassouna, Rim
author_facet Hassouna, Rim
Fernandez, Gimena
Lebrun, Nicolas
Fiquet, Oriane
Roelfsema, Ferdinand
Labarthe, Alexandra
Zizzari, Philippe
Tomasetto, Catherine
Epelbaum, Jacques
Viltart, Odile
Chauveau, Christophe
Perello, Mario
Tolle, Virginie
author_role author
author2 Fernandez, Gimena
Lebrun, Nicolas
Fiquet, Oriane
Roelfsema, Ferdinand
Labarthe, Alexandra
Zizzari, Philippe
Tomasetto, Catherine
Epelbaum, Jacques
Viltart, Odile
Chauveau, Christophe
Perello, Mario
Tolle, Virginie
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv FOOD INTAKE
GHRELIN
GROWTH HORMONE
PULSATILITY
SEXUAL DIMORPHISM
topic FOOD INTAKE
GHRELIN
GROWTH HORMONE
PULSATILITY
SEXUAL DIMORPHISM
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Using preproghrelin-deficient mice (Ghrl-/-), we previously observed that preproghrelin modulates pulsatile growth hormone (GH) secretion in post-pubertal male mice. However, the role of ghrelin and its derived peptides in the regulation of growth parameters or feeding in females is unknown. We measured pulsatile GH secretion, growth, metabolic parameters and feeding behavior in adult Ghrl-/- and Ghrl+/+ male and female mice. We also assessed GH release from pituitary explants and hypothalamic growth hormonereleasing hormone (GHRH) expression and immunoreactivity. Body weight and body fat mass, linear growth, spontaneous food intake and food intake following a 48-h fast, GH pituitary contents and GH release from pituitary explants ex vivo, fasting glucose and glucose tolerance were not different among adult Ghrl-/- and Ghrl+/+ male or female mice. In vivo, pulsatile GH secretion was decreased, while approximate entropy, that quantified orderliness of secretion, was increased in adult Ghrl-/- females only, defining more irregular GH pattern. The number of neurons immunoreactive for GHRH visualized in the hypothalamic arcuate nucleus was increased in adult Ghrl-/- females, as compared to Ghrl+/+ females, whereas the expression of GHRH was not different amongst groups. Thus, these results point to sex-specific effects of preproghrelin gene deletion on pulsatile GH secretion, but not feeding, growth or metabolic parameters, in adult mice.
Fil: Hassouna, Rim. Université Paris Cité; Francia. Universite de Bordeaux; Francia. Inserm; Francia
Fil: Fernandez, Gimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; Argentina
Fil: Lebrun, Nicolas. Université Paris Cité; Francia
Fil: Fiquet, Oriane. Universite de Bordeaux; Francia. Université Paris Cité; Francia. Inserm; Francia
Fil: Roelfsema, Ferdinand. Leiden University Medical Center; Países Bajos
Fil: Labarthe, Alexandra. Université Paris Cité; Francia. Universite de Bordeaux; Francia. Inserm; Francia
Fil: Zizzari, Philippe. Universite de Bordeaux; Francia. Université Paris Cité; Francia. Inserm; Francia
Fil: Tomasetto, Catherine. Universite de Bordeaux; Francia. Université Paris Cité; Francia. Inserm; Francia
Fil: Epelbaum, Jacques. Universite de Bordeaux; Francia. Université Paris Cité; Francia. Inserm; Francia
Fil: Viltart, Odile. Universite de Bordeaux; Francia. Université Paris Cité; Francia. Inserm; Francia
Fil: Chauveau, Christophe. Université Du Littoral Côte D‘opale; Francia
Fil: Perello, Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; Argentina
Fil: Tolle, Virginie. Université Paris Cité; Francia
description Using preproghrelin-deficient mice (Ghrl-/-), we previously observed that preproghrelin modulates pulsatile growth hormone (GH) secretion in post-pubertal male mice. However, the role of ghrelin and its derived peptides in the regulation of growth parameters or feeding in females is unknown. We measured pulsatile GH secretion, growth, metabolic parameters and feeding behavior in adult Ghrl-/- and Ghrl+/+ male and female mice. We also assessed GH release from pituitary explants and hypothalamic growth hormonereleasing hormone (GHRH) expression and immunoreactivity. Body weight and body fat mass, linear growth, spontaneous food intake and food intake following a 48-h fast, GH pituitary contents and GH release from pituitary explants ex vivo, fasting glucose and glucose tolerance were not different among adult Ghrl-/- and Ghrl+/+ male or female mice. In vivo, pulsatile GH secretion was decreased, while approximate entropy, that quantified orderliness of secretion, was increased in adult Ghrl-/- females only, defining more irregular GH pattern. The number of neurons immunoreactive for GHRH visualized in the hypothalamic arcuate nucleus was increased in adult Ghrl-/- females, as compared to Ghrl+/+ females, whereas the expression of GHRH was not different amongst groups. Thus, these results point to sex-specific effects of preproghrelin gene deletion on pulsatile GH secretion, but not feeding, growth or metabolic parameters, in adult mice.
publishDate 2021
dc.date.none.fl_str_mv 2021-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/181352
Hassouna, Rim; Fernandez, Gimena; Lebrun, Nicolas; Fiquet, Oriane; Roelfsema, Ferdinand; et al.; Ghrelin Gene Deletion Alters Pulsatile Growth Hormone Secretion in Adult Female Mice; Frontiers Media; Frontiers in Endocrinology; 12; 754522; 10-2021; 1-11
1664-2392
CONICET Digital
CONICET
url http://hdl.handle.net/11336/181352
identifier_str_mv Hassouna, Rim; Fernandez, Gimena; Lebrun, Nicolas; Fiquet, Oriane; Roelfsema, Ferdinand; et al.; Ghrelin Gene Deletion Alters Pulsatile Growth Hormone Secretion in Adult Female Mice; Frontiers Media; Frontiers in Endocrinology; 12; 754522; 10-2021; 1-11
1664-2392
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fendo.2021.754522/full
info:eu-repo/semantics/altIdentifier/doi/10.3389/fendo.2021.754522
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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