Agrowastes as Feedstock for the Production of Endo-β-Xylanase from Cohnella sp. Strain AR92

Autores
Pisa, José Horacio; Manfredi, Adriana Paola; Perotti, Nora Ines; Romero, Héctor G.; Breccia, Javier Dario; Martinez, Maria Alejandra
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Members of Cohnella sp. isolated from a variety of environments have been shown to be glycoside hydrolase producers. Nevertheless, most evaluations of members of this genus are limited to their taxonomic description. The strain AR92, previously identified as belonging to the genus Cohnella, formed a well-supported cluster with C. thailandensis and C. formosensis (>80% bootstrap confidence). Its growth and xylanase production were approached by using a mineral-based medium containing alkali-pretreated sugarcane bagasse as the main carbon source, which was assayed as a convenient source to produce biocatalysts potentially fitting its degradation. By means of a two-step statistical approach, the production of endoxylanase was moderately improved (20%). However, a far more significant improvement was observed (145%), by increasing the inoculum size and lowering the fermentation temperature to 25°C, which is below the optimal growth temperature of the strain AR92 (37°C). The xylanolytic preparation produced by Cohnella sp. AR92 contained mild temperature-active endoxylanase (identified as redundant GH10 family) for the main activity which resulted in xylobiose and xylo-oligosaccharides as the main products from birchwood xylan.
Fil: Pisa, José Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Manfredi, Adriana Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Perotti, Nora Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Romero, Héctor G.. Universidad de la República. Facultad de Ciencias; Uruguay
Fil: Breccia, Javier Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias de la Tierra y Ambientales de La Pampa. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Instituto de Ciencias de la Tierra y Ambientales de La Pampa; Argentina
Fil: Martinez, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Materia
Cohnella
Hemicellulose Degradation
Paenibacillaceae
Sugarcane Bagasse
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/39560
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/39560
network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Agrowastes as Feedstock for the Production of Endo-β-Xylanase from Cohnella sp. Strain AR92Pisa, José HoracioManfredi, Adriana PaolaPerotti, Nora InesRomero, Héctor G.Breccia, Javier DarioMartinez, Maria AlejandraCohnellaHemicellulose DegradationPaenibacillaceaeSugarcane Bagassehttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2Members of Cohnella sp. isolated from a variety of environments have been shown to be glycoside hydrolase producers. Nevertheless, most evaluations of members of this genus are limited to their taxonomic description. The strain AR92, previously identified as belonging to the genus Cohnella, formed a well-supported cluster with C. thailandensis and C. formosensis (>80% bootstrap confidence). Its growth and xylanase production were approached by using a mineral-based medium containing alkali-pretreated sugarcane bagasse as the main carbon source, which was assayed as a convenient source to produce biocatalysts potentially fitting its degradation. By means of a two-step statistical approach, the production of endoxylanase was moderately improved (20%). However, a far more significant improvement was observed (145%), by increasing the inoculum size and lowering the fermentation temperature to 25°C, which is below the optimal growth temperature of the strain AR92 (37°C). The xylanolytic preparation produced by Cohnella sp. AR92 contained mild temperature-active endoxylanase (identified as redundant GH10 family) for the main activity which resulted in xylobiose and xylo-oligosaccharides as the main products from birchwood xylan.Fil: Pisa, José Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Manfredi, Adriana Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Perotti, Nora Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Romero, Héctor G.. Universidad de la República. Facultad de Ciencias; UruguayFil: Breccia, Javier Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias de la Tierra y Ambientales de La Pampa. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Instituto de Ciencias de la Tierra y Ambientales de La Pampa; ArgentinaFil: Martinez, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaKarger2017-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/39560Pisa, José Horacio; Manfredi, Adriana Paola; Perotti, Nora Ines; Romero, Héctor G.; Breccia, Javier Dario; et al.; Agrowastes as Feedstock for the Production of Endo-β-Xylanase from Cohnella sp. Strain AR92; Karger; Journal of Molecular Microbiology and Biotechnology; 27; 5; 11-2017; 277-2881464-1801CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.karger.com/Article/Abstract/480541info:eu-repo/semantics/altIdentifier/doi/10.1159/000480541info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:45:51Zoai:ri.conicet.gov.ar:11336/39560instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:45:51.702CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Agrowastes as Feedstock for the Production of Endo-β-Xylanase from Cohnella sp. Strain AR92
title Agrowastes as Feedstock for the Production of Endo-β-Xylanase from Cohnella sp. Strain AR92
spellingShingle Agrowastes as Feedstock for the Production of Endo-β-Xylanase from Cohnella sp. Strain AR92
Pisa, José Horacio
Cohnella
Hemicellulose Degradation
Paenibacillaceae
Sugarcane Bagasse
title_short Agrowastes as Feedstock for the Production of Endo-β-Xylanase from Cohnella sp. Strain AR92
title_full Agrowastes as Feedstock for the Production of Endo-β-Xylanase from Cohnella sp. Strain AR92
title_fullStr Agrowastes as Feedstock for the Production of Endo-β-Xylanase from Cohnella sp. Strain AR92
title_full_unstemmed Agrowastes as Feedstock for the Production of Endo-β-Xylanase from Cohnella sp. Strain AR92
title_sort Agrowastes as Feedstock for the Production of Endo-β-Xylanase from Cohnella sp. Strain AR92
dc.creator.none.fl_str_mv Pisa, José Horacio
Manfredi, Adriana Paola
Perotti, Nora Ines
Romero, Héctor G.
Breccia, Javier Dario
Martinez, Maria Alejandra
author Pisa, José Horacio
author_facet Pisa, José Horacio
Manfredi, Adriana Paola
Perotti, Nora Ines
Romero, Héctor G.
Breccia, Javier Dario
Martinez, Maria Alejandra
author_role author
author2 Manfredi, Adriana Paola
Perotti, Nora Ines
Romero, Héctor G.
Breccia, Javier Dario
Martinez, Maria Alejandra
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Cohnella
Hemicellulose Degradation
Paenibacillaceae
Sugarcane Bagasse
topic Cohnella
Hemicellulose Degradation
Paenibacillaceae
Sugarcane Bagasse
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Members of Cohnella sp. isolated from a variety of environments have been shown to be glycoside hydrolase producers. Nevertheless, most evaluations of members of this genus are limited to their taxonomic description. The strain AR92, previously identified as belonging to the genus Cohnella, formed a well-supported cluster with C. thailandensis and C. formosensis (>80% bootstrap confidence). Its growth and xylanase production were approached by using a mineral-based medium containing alkali-pretreated sugarcane bagasse as the main carbon source, which was assayed as a convenient source to produce biocatalysts potentially fitting its degradation. By means of a two-step statistical approach, the production of endoxylanase was moderately improved (20%). However, a far more significant improvement was observed (145%), by increasing the inoculum size and lowering the fermentation temperature to 25°C, which is below the optimal growth temperature of the strain AR92 (37°C). The xylanolytic preparation produced by Cohnella sp. AR92 contained mild temperature-active endoxylanase (identified as redundant GH10 family) for the main activity which resulted in xylobiose and xylo-oligosaccharides as the main products from birchwood xylan.
Fil: Pisa, José Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Manfredi, Adriana Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Perotti, Nora Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Romero, Héctor G.. Universidad de la República. Facultad de Ciencias; Uruguay
Fil: Breccia, Javier Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias de la Tierra y Ambientales de La Pampa. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Instituto de Ciencias de la Tierra y Ambientales de La Pampa; Argentina
Fil: Martinez, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
description Members of Cohnella sp. isolated from a variety of environments have been shown to be glycoside hydrolase producers. Nevertheless, most evaluations of members of this genus are limited to their taxonomic description. The strain AR92, previously identified as belonging to the genus Cohnella, formed a well-supported cluster with C. thailandensis and C. formosensis (>80% bootstrap confidence). Its growth and xylanase production were approached by using a mineral-based medium containing alkali-pretreated sugarcane bagasse as the main carbon source, which was assayed as a convenient source to produce biocatalysts potentially fitting its degradation. By means of a two-step statistical approach, the production of endoxylanase was moderately improved (20%). However, a far more significant improvement was observed (145%), by increasing the inoculum size and lowering the fermentation temperature to 25°C, which is below the optimal growth temperature of the strain AR92 (37°C). The xylanolytic preparation produced by Cohnella sp. AR92 contained mild temperature-active endoxylanase (identified as redundant GH10 family) for the main activity which resulted in xylobiose and xylo-oligosaccharides as the main products from birchwood xylan.
publishDate 2017
dc.date.none.fl_str_mv 2017-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/39560
Pisa, José Horacio; Manfredi, Adriana Paola; Perotti, Nora Ines; Romero, Héctor G.; Breccia, Javier Dario; et al.; Agrowastes as Feedstock for the Production of Endo-β-Xylanase from Cohnella sp. Strain AR92; Karger; Journal of Molecular Microbiology and Biotechnology; 27; 5; 11-2017; 277-288
1464-1801
CONICET Digital
CONICET
url http://hdl.handle.net/11336/39560
identifier_str_mv Pisa, José Horacio; Manfredi, Adriana Paola; Perotti, Nora Ines; Romero, Héctor G.; Breccia, Javier Dario; et al.; Agrowastes as Feedstock for the Production of Endo-β-Xylanase from Cohnella sp. Strain AR92; Karger; Journal of Molecular Microbiology and Biotechnology; 27; 5; 11-2017; 277-288
1464-1801
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.karger.com/Article/Abstract/480541
info:eu-repo/semantics/altIdentifier/doi/10.1159/000480541
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Karger
publisher.none.fl_str_mv Karger
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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