Evaluation of biofilm-forming capacity of Moraxella bovis, the primary causative agent of infectious bovine keratoconjunctivitis

Autores
Prieto, Claudia Inés; Serra, Diego Omar; Martina, Pablo F.; Jacobs, Melisa; Bosch, María Alejandra; Yantorno, Osvaldo Miguel
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The difficulties in preventing and treating infectious bovine keratoconjunctivitis (IBK) and the consequent impact on the cattle industry worldwide emphasize the need to better understand this infectious process along with the biology of Moraxella bovis, its primary causative agent. Although there is increasing evidence that bacterial biofilms participate in a variety of ocular infections by direct biofilm formation on the surfaces of the eye, IBK has not been considered as a biofilm-based disease so far, and even more, no information is currently available regarding the ability of M. bovis to adopt a biofilm lifestyle. In the present research, we demonstrated the capacity of M. bovis clinical isolates and reference strains to form biofilms on different abiotic surfaces and culture conditions, and provided qualitative and quantitative information on the biofilm growth and architecture of mature biofilms. In addition, our data indicated that the type IV pili play a critical role in the biofilm formation in vitro. Most significantly, we proved that through exposure to MgCl2 type IV pili are removed from the cell surface, not only preventing M. bovis biofilm formation but also disassembling preformed biofilms. These results could constitute a new approach in the understanding of M. bovis colonization process in cattle eye and/or nasal cavity, and may aid in the development of future antimicrobial strategies for the control of IBK. © 2013 Elsevier B.V.
Fil: Prieto, Claudia Inés. Universidad Nacional de La Plata; Argentina
Fil: Serra, Diego Omar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Martina, Pablo F.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Jacobs, Melisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Bosch, María Alejandra. Universidad Nacional de La Plata; Argentina
Fil: Yantorno, Osvaldo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Materia
ANTIBIOTIC RESISTANCE
BIOFILM
DISASSEMBLING AGENTS
INFECTIOUS BOVINE KERATOCONJUNCTIVITIS
MORAXELLA BOVIS
TYPE IV PILI
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/76765
Acceder
id CONICETDig_9f2f250243f4437be38641ec521a737d
oai_identifier_str oai:ri.conicet.gov.ar:11336/76765
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Evaluation of biofilm-forming capacity of Moraxella bovis, the primary causative agent of infectious bovine keratoconjunctivitisPrieto, Claudia InésSerra, Diego OmarMartina, Pablo F.Jacobs, MelisaBosch, María AlejandraYantorno, Osvaldo MiguelANTIBIOTIC RESISTANCEBIOFILMDISASSEMBLING AGENTSINFECTIOUS BOVINE KERATOCONJUNCTIVITISMORAXELLA BOVISTYPE IV PILIhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The difficulties in preventing and treating infectious bovine keratoconjunctivitis (IBK) and the consequent impact on the cattle industry worldwide emphasize the need to better understand this infectious process along with the biology of Moraxella bovis, its primary causative agent. Although there is increasing evidence that bacterial biofilms participate in a variety of ocular infections by direct biofilm formation on the surfaces of the eye, IBK has not been considered as a biofilm-based disease so far, and even more, no information is currently available regarding the ability of M. bovis to adopt a biofilm lifestyle. In the present research, we demonstrated the capacity of M. bovis clinical isolates and reference strains to form biofilms on different abiotic surfaces and culture conditions, and provided qualitative and quantitative information on the biofilm growth and architecture of mature biofilms. In addition, our data indicated that the type IV pili play a critical role in the biofilm formation in vitro. Most significantly, we proved that through exposure to MgCl2 type IV pili are removed from the cell surface, not only preventing M. bovis biofilm formation but also disassembling preformed biofilms. These results could constitute a new approach in the understanding of M. bovis colonization process in cattle eye and/or nasal cavity, and may aid in the development of future antimicrobial strategies for the control of IBK. © 2013 Elsevier B.V.Fil: Prieto, Claudia Inés. Universidad Nacional de La Plata; ArgentinaFil: Serra, Diego Omar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Martina, Pablo F.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Jacobs, Melisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Bosch, María Alejandra. Universidad Nacional de La Plata; ArgentinaFil: Yantorno, Osvaldo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaElsevier Science2013-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/76765Prieto, Claudia Inés; Serra, Diego Omar; Martina, Pablo F.; Jacobs, Melisa; Bosch, María Alejandra; et al.; Evaluation of biofilm-forming capacity of Moraxella bovis, the primary causative agent of infectious bovine keratoconjunctivitis; Elsevier Science; Veterinary Microbiology; 166; 3-4; 10-2013; 504-5150378-1135CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.vetmic.2013.06.003info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0378113513003143info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:10:31Zoai:ri.conicet.gov.ar:11336/76765instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:10:31.462CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Evaluation of biofilm-forming capacity of Moraxella bovis, the primary causative agent of infectious bovine keratoconjunctivitis
title Evaluation of biofilm-forming capacity of Moraxella bovis, the primary causative agent of infectious bovine keratoconjunctivitis
spellingShingle Evaluation of biofilm-forming capacity of Moraxella bovis, the primary causative agent of infectious bovine keratoconjunctivitis
Prieto, Claudia Inés
ANTIBIOTIC RESISTANCE
BIOFILM
DISASSEMBLING AGENTS
INFECTIOUS BOVINE KERATOCONJUNCTIVITIS
MORAXELLA BOVIS
TYPE IV PILI
title_short Evaluation of biofilm-forming capacity of Moraxella bovis, the primary causative agent of infectious bovine keratoconjunctivitis
title_full Evaluation of biofilm-forming capacity of Moraxella bovis, the primary causative agent of infectious bovine keratoconjunctivitis
title_fullStr Evaluation of biofilm-forming capacity of Moraxella bovis, the primary causative agent of infectious bovine keratoconjunctivitis
title_full_unstemmed Evaluation of biofilm-forming capacity of Moraxella bovis, the primary causative agent of infectious bovine keratoconjunctivitis
title_sort Evaluation of biofilm-forming capacity of Moraxella bovis, the primary causative agent of infectious bovine keratoconjunctivitis
dc.creator.none.fl_str_mv Prieto, Claudia Inés
Serra, Diego Omar
Martina, Pablo F.
Jacobs, Melisa
Bosch, María Alejandra
Yantorno, Osvaldo Miguel
author Prieto, Claudia Inés
author_facet Prieto, Claudia Inés
Serra, Diego Omar
Martina, Pablo F.
Jacobs, Melisa
Bosch, María Alejandra
Yantorno, Osvaldo Miguel
author_role author
author2 Serra, Diego Omar
Martina, Pablo F.
Jacobs, Melisa
Bosch, María Alejandra
Yantorno, Osvaldo Miguel
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv ANTIBIOTIC RESISTANCE
BIOFILM
DISASSEMBLING AGENTS
INFECTIOUS BOVINE KERATOCONJUNCTIVITIS
MORAXELLA BOVIS
TYPE IV PILI
topic ANTIBIOTIC RESISTANCE
BIOFILM
DISASSEMBLING AGENTS
INFECTIOUS BOVINE KERATOCONJUNCTIVITIS
MORAXELLA BOVIS
TYPE IV PILI
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The difficulties in preventing and treating infectious bovine keratoconjunctivitis (IBK) and the consequent impact on the cattle industry worldwide emphasize the need to better understand this infectious process along with the biology of Moraxella bovis, its primary causative agent. Although there is increasing evidence that bacterial biofilms participate in a variety of ocular infections by direct biofilm formation on the surfaces of the eye, IBK has not been considered as a biofilm-based disease so far, and even more, no information is currently available regarding the ability of M. bovis to adopt a biofilm lifestyle. In the present research, we demonstrated the capacity of M. bovis clinical isolates and reference strains to form biofilms on different abiotic surfaces and culture conditions, and provided qualitative and quantitative information on the biofilm growth and architecture of mature biofilms. In addition, our data indicated that the type IV pili play a critical role in the biofilm formation in vitro. Most significantly, we proved that through exposure to MgCl2 type IV pili are removed from the cell surface, not only preventing M. bovis biofilm formation but also disassembling preformed biofilms. These results could constitute a new approach in the understanding of M. bovis colonization process in cattle eye and/or nasal cavity, and may aid in the development of future antimicrobial strategies for the control of IBK. © 2013 Elsevier B.V.
Fil: Prieto, Claudia Inés. Universidad Nacional de La Plata; Argentina
Fil: Serra, Diego Omar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Martina, Pablo F.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Jacobs, Melisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
Fil: Bosch, María Alejandra. Universidad Nacional de La Plata; Argentina
Fil: Yantorno, Osvaldo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
description The difficulties in preventing and treating infectious bovine keratoconjunctivitis (IBK) and the consequent impact on the cattle industry worldwide emphasize the need to better understand this infectious process along with the biology of Moraxella bovis, its primary causative agent. Although there is increasing evidence that bacterial biofilms participate in a variety of ocular infections by direct biofilm formation on the surfaces of the eye, IBK has not been considered as a biofilm-based disease so far, and even more, no information is currently available regarding the ability of M. bovis to adopt a biofilm lifestyle. In the present research, we demonstrated the capacity of M. bovis clinical isolates and reference strains to form biofilms on different abiotic surfaces and culture conditions, and provided qualitative and quantitative information on the biofilm growth and architecture of mature biofilms. In addition, our data indicated that the type IV pili play a critical role in the biofilm formation in vitro. Most significantly, we proved that through exposure to MgCl2 type IV pili are removed from the cell surface, not only preventing M. bovis biofilm formation but also disassembling preformed biofilms. These results could constitute a new approach in the understanding of M. bovis colonization process in cattle eye and/or nasal cavity, and may aid in the development of future antimicrobial strategies for the control of IBK. © 2013 Elsevier B.V.
publishDate 2013
dc.date.none.fl_str_mv 2013-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/76765
Prieto, Claudia Inés; Serra, Diego Omar; Martina, Pablo F.; Jacobs, Melisa; Bosch, María Alejandra; et al.; Evaluation of biofilm-forming capacity of Moraxella bovis, the primary causative agent of infectious bovine keratoconjunctivitis; Elsevier Science; Veterinary Microbiology; 166; 3-4; 10-2013; 504-515
0378-1135
CONICET Digital
CONICET
url http://hdl.handle.net/11336/76765
identifier_str_mv Prieto, Claudia Inés; Serra, Diego Omar; Martina, Pablo F.; Jacobs, Melisa; Bosch, María Alejandra; et al.; Evaluation of biofilm-forming capacity of Moraxella bovis, the primary causative agent of infectious bovine keratoconjunctivitis; Elsevier Science; Veterinary Microbiology; 166; 3-4; 10-2013; 504-515
0378-1135
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.vetmic.2013.06.003
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0378113513003143
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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