Crosses between monokaryons of Pleurotus sapidus or Pleurotus florida show an improved biotransformation of (+)-valencene to (+)-nootkatone

Autores
Omarini, Alejandra Beatriz; Plagemann, Ina; Schimanski, Silke; Krings, Ulrich; Berger, Ralf G.
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Several hundred monokaryotic and new dikaryotic strains derived thereof were established from (+)-valencene tolerant Pleurotus species. When grouped according to their growth rate on agar plates and compared to the parental of Pleurotus sapidus 69, the slowly growing monokaryons converted (+)-valencene more efficiently to the grapefruit flavour compound (+)-nootkatone. The fast growing monokaryons and the slow × slow and the fast × fast dikaryotic crosses showed similar or inferior yields. Some slow × fast dikaryons, however, exceeded the biotransformation capability of the parental dikaryon significantly. The activity of the responsible enzyme, lipoxygenase, showed a weak correlation with the yields of (+)-nootkatone indicating that the determination of enzyme activity using the primary substrate linoleic acid may be misleading in predicting the biotransformation efficiency. This exploratory study indicated that a classical genetics approach resulted in altered and partly improved terpene transformation capability (plus 60%) and lipoxygenase activity of the strains.
Fil: Omarini, Alejandra Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Leibniz Universität Hannover. Hannover; Alemania
Fil: Plagemann, Ina. Leibniz Universität Hannover. Hannover; Alemania
Fil: Schimanski, Silke. Leibniz Universität Hannover. Hannover; Alemania
Fil: Krings, Ulrich. Leibniz Universität Hannover. Hannover; Alemania
Fil: Berger, Ralf G.. Leibniz Universität Hannover. Hannover; Alemania
Materia
Pleurotus
Basidiomycota
Monokaryon
(+)-Valencene
Biotransformation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/35925
Acceder
id CONICETDig_9e523c607171fec4207d93ca70ad36e5
oai_identifier_str oai:ri.conicet.gov.ar:11336/35925
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Crosses between monokaryons of Pleurotus sapidus or Pleurotus florida show an improved biotransformation of (+)-valencene to (+)-nootkatoneOmarini, Alejandra BeatrizPlagemann, InaSchimanski, SilkeKrings, UlrichBerger, Ralf G.PleurotusBasidiomycotaMonokaryon(+)-ValenceneBiotransformationhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Several hundred monokaryotic and new dikaryotic strains derived thereof were established from (+)-valencene tolerant Pleurotus species. When grouped according to their growth rate on agar plates and compared to the parental of Pleurotus sapidus 69, the slowly growing monokaryons converted (+)-valencene more efficiently to the grapefruit flavour compound (+)-nootkatone. The fast growing monokaryons and the slow × slow and the fast × fast dikaryotic crosses showed similar or inferior yields. Some slow × fast dikaryons, however, exceeded the biotransformation capability of the parental dikaryon significantly. The activity of the responsible enzyme, lipoxygenase, showed a weak correlation with the yields of (+)-nootkatone indicating that the determination of enzyme activity using the primary substrate linoleic acid may be misleading in predicting the biotransformation efficiency. This exploratory study indicated that a classical genetics approach resulted in altered and partly improved terpene transformation capability (plus 60%) and lipoxygenase activity of the strains.Fil: Omarini, Alejandra Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Leibniz Universität Hannover. Hannover; AlemaniaFil: Plagemann, Ina. Leibniz Universität Hannover. Hannover; AlemaniaFil: Schimanski, Silke. Leibniz Universität Hannover. Hannover; AlemaniaFil: Krings, Ulrich. Leibniz Universität Hannover. Hannover; AlemaniaFil: Berger, Ralf G.. Leibniz Universität Hannover. Hannover; AlemaniaElsevier2014-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/35925Omarini, Alejandra Beatriz; Plagemann, Ina ; Schimanski, Silke; Krings, Ulrich ; Berger, Ralf G.; Crosses between monokaryons of Pleurotus sapidus or Pleurotus florida show an improved biotransformation of (+)-valencene to (+)-nootkatone; Elsevier; Bioresource Technology; 171; 8-2014; 113-1190960-8524CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.biortech.2014.08.061info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0960852414011778info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:52:04Zoai:ri.conicet.gov.ar:11336/35925instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:52:04.901CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Crosses between monokaryons of Pleurotus sapidus or Pleurotus florida show an improved biotransformation of (+)-valencene to (+)-nootkatone
title Crosses between monokaryons of Pleurotus sapidus or Pleurotus florida show an improved biotransformation of (+)-valencene to (+)-nootkatone
spellingShingle Crosses between monokaryons of Pleurotus sapidus or Pleurotus florida show an improved biotransformation of (+)-valencene to (+)-nootkatone
Omarini, Alejandra Beatriz
Pleurotus
Basidiomycota
Monokaryon
(+)-Valencene
Biotransformation
title_short Crosses between monokaryons of Pleurotus sapidus or Pleurotus florida show an improved biotransformation of (+)-valencene to (+)-nootkatone
title_full Crosses between monokaryons of Pleurotus sapidus or Pleurotus florida show an improved biotransformation of (+)-valencene to (+)-nootkatone
title_fullStr Crosses between monokaryons of Pleurotus sapidus or Pleurotus florida show an improved biotransformation of (+)-valencene to (+)-nootkatone
title_full_unstemmed Crosses between monokaryons of Pleurotus sapidus or Pleurotus florida show an improved biotransformation of (+)-valencene to (+)-nootkatone
title_sort Crosses between monokaryons of Pleurotus sapidus or Pleurotus florida show an improved biotransformation of (+)-valencene to (+)-nootkatone
dc.creator.none.fl_str_mv Omarini, Alejandra Beatriz
Plagemann, Ina
Schimanski, Silke
Krings, Ulrich
Berger, Ralf G.
author Omarini, Alejandra Beatriz
author_facet Omarini, Alejandra Beatriz
Plagemann, Ina
Schimanski, Silke
Krings, Ulrich
Berger, Ralf G.
author_role author
author2 Plagemann, Ina
Schimanski, Silke
Krings, Ulrich
Berger, Ralf G.
author2_role author
author
author
author
dc.subject.none.fl_str_mv Pleurotus
Basidiomycota
Monokaryon
(+)-Valencene
Biotransformation
topic Pleurotus
Basidiomycota
Monokaryon
(+)-Valencene
Biotransformation
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Several hundred monokaryotic and new dikaryotic strains derived thereof were established from (+)-valencene tolerant Pleurotus species. When grouped according to their growth rate on agar plates and compared to the parental of Pleurotus sapidus 69, the slowly growing monokaryons converted (+)-valencene more efficiently to the grapefruit flavour compound (+)-nootkatone. The fast growing monokaryons and the slow × slow and the fast × fast dikaryotic crosses showed similar or inferior yields. Some slow × fast dikaryons, however, exceeded the biotransformation capability of the parental dikaryon significantly. The activity of the responsible enzyme, lipoxygenase, showed a weak correlation with the yields of (+)-nootkatone indicating that the determination of enzyme activity using the primary substrate linoleic acid may be misleading in predicting the biotransformation efficiency. This exploratory study indicated that a classical genetics approach resulted in altered and partly improved terpene transformation capability (plus 60%) and lipoxygenase activity of the strains.
Fil: Omarini, Alejandra Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Leibniz Universität Hannover. Hannover; Alemania
Fil: Plagemann, Ina. Leibniz Universität Hannover. Hannover; Alemania
Fil: Schimanski, Silke. Leibniz Universität Hannover. Hannover; Alemania
Fil: Krings, Ulrich. Leibniz Universität Hannover. Hannover; Alemania
Fil: Berger, Ralf G.. Leibniz Universität Hannover. Hannover; Alemania
description Several hundred monokaryotic and new dikaryotic strains derived thereof were established from (+)-valencene tolerant Pleurotus species. When grouped according to their growth rate on agar plates and compared to the parental of Pleurotus sapidus 69, the slowly growing monokaryons converted (+)-valencene more efficiently to the grapefruit flavour compound (+)-nootkatone. The fast growing monokaryons and the slow × slow and the fast × fast dikaryotic crosses showed similar or inferior yields. Some slow × fast dikaryons, however, exceeded the biotransformation capability of the parental dikaryon significantly. The activity of the responsible enzyme, lipoxygenase, showed a weak correlation with the yields of (+)-nootkatone indicating that the determination of enzyme activity using the primary substrate linoleic acid may be misleading in predicting the biotransformation efficiency. This exploratory study indicated that a classical genetics approach resulted in altered and partly improved terpene transformation capability (plus 60%) and lipoxygenase activity of the strains.
publishDate 2014
dc.date.none.fl_str_mv 2014-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/35925
Omarini, Alejandra Beatriz; Plagemann, Ina ; Schimanski, Silke; Krings, Ulrich ; Berger, Ralf G.; Crosses between monokaryons of Pleurotus sapidus or Pleurotus florida show an improved biotransformation of (+)-valencene to (+)-nootkatone; Elsevier; Bioresource Technology; 171; 8-2014; 113-119
0960-8524
CONICET Digital
CONICET
url http://hdl.handle.net/11336/35925
identifier_str_mv Omarini, Alejandra Beatriz; Plagemann, Ina ; Schimanski, Silke; Krings, Ulrich ; Berger, Ralf G.; Crosses between monokaryons of Pleurotus sapidus or Pleurotus florida show an improved biotransformation of (+)-valencene to (+)-nootkatone; Elsevier; Bioresource Technology; 171; 8-2014; 113-119
0960-8524
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.biortech.2014.08.061
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0960852414011778
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613599213912064
score 13.070432

Publicaciones similares