Monokaryotic pleurotus sapidus strains with intraspecific variability of an alkene cleaving dyp-type peroxidase activity as a result of gene mutation and differential gene expressi...

Autores
Krahe, NinaKatharina; Berger, Ralf G.; Witt, Martin; Zorn, Holger; Omarini, Alejandra Beatriz; Ersoy, Franziska
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The basidiomycete Pleurotus sapidus produced a dye-decolorizing peroxidase (PsaPOX) with alkene cleavage activity, implying potential as a biocatalyst for the fragrance and flavor industry. To increase the activity, a daughter-generation of 101 basidiospore-derived monokaryons (MK) was used. After a pre-selection according to the growth rate, the activity analysis revealed a stable intraspecific variability of the strains regarding peroxidase and alkene cleavage activity of PsaPOX. Ten monokaryons reached activities up to 2.6-fold higher than the dikaryon, with MK16 showing the highest activity. Analysis of the PsaPOX gene identified three different enzyme variants. These were co-responsible for the observed differences in activities between strains as verified by heterologous expression in Komagataella phaffii. The mutation S371H in enzyme variant PsaPOX_high caused an activity increase alongside a higher protein stability, while the eleven mutations in variant PsaPOX_low resulted in an activity decrease, which was partially based on a shift of the pH optimum from 3.5 to 3.0. Transcriptional analysis revealed the increased expression of PsaPOX in MK16 as reason for the higher PsaPOX activity in comparison to other strains producing the same PsaPOX variant. Thus, different expression profiles, as well as enzyme variants, were identified as crucial factors for the intraspecific variability of the PsaPOX activity in the monokaryons.
Fil: Krahe, NinaKatharina. Leibniz Universitat Hannover; Alemania
Fil: Berger, Ralf G.. Leibniz Universitat Hannover; Alemania
Fil: Witt, Martin. Leibniz Universitat Hannover; Alemania
Fil: Zorn, Holger. Leibniz Universitat Hannover; Alemania
Fil: Omarini, Alejandra Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias de la Tierra y Ambientales de La Pampa. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Instituto de Ciencias de la Tierra y Ambientales de La Pampa; Argentina
Fil: Ersoy, Franziska. Leibniz Universitat Hannover; Alemania
Materia
ALKENE CLEAVAGE
BASIDIOMYCOTA
BIOCATALYSIS
DIKARYON
DYE-DECOLORIZING PEROXIDASE (DYP)
GENE EXPRESSION
GENE MUTATION
INTRASPECIFIC VARIABILITY
MONOKARYON
PLEUROTUS SAPIDUS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/183442
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/183442
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Monokaryotic pleurotus sapidus strains with intraspecific variability of an alkene cleaving dyp-type peroxidase activity as a result of gene mutation and differential gene expressionKrahe, NinaKatharinaBerger, Ralf G.Witt, MartinZorn, HolgerOmarini, Alejandra BeatrizErsoy, FranziskaALKENE CLEAVAGEBASIDIOMYCOTABIOCATALYSISDIKARYONDYE-DECOLORIZING PEROXIDASE (DYP)GENE EXPRESSIONGENE MUTATIONINTRASPECIFIC VARIABILITYMONOKARYONPLEUROTUS SAPIDUShttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2The basidiomycete Pleurotus sapidus produced a dye-decolorizing peroxidase (PsaPOX) with alkene cleavage activity, implying potential as a biocatalyst for the fragrance and flavor industry. To increase the activity, a daughter-generation of 101 basidiospore-derived monokaryons (MK) was used. After a pre-selection according to the growth rate, the activity analysis revealed a stable intraspecific variability of the strains regarding peroxidase and alkene cleavage activity of PsaPOX. Ten monokaryons reached activities up to 2.6-fold higher than the dikaryon, with MK16 showing the highest activity. Analysis of the PsaPOX gene identified three different enzyme variants. These were co-responsible for the observed differences in activities between strains as verified by heterologous expression in Komagataella phaffii. The mutation S371H in enzyme variant PsaPOX_high caused an activity increase alongside a higher protein stability, while the eleven mutations in variant PsaPOX_low resulted in an activity decrease, which was partially based on a shift of the pH optimum from 3.5 to 3.0. Transcriptional analysis revealed the increased expression of PsaPOX in MK16 as reason for the higher PsaPOX activity in comparison to other strains producing the same PsaPOX variant. Thus, different expression profiles, as well as enzyme variants, were identified as crucial factors for the intraspecific variability of the PsaPOX activity in the monokaryons.Fil: Krahe, NinaKatharina. Leibniz Universitat Hannover; AlemaniaFil: Berger, Ralf G.. Leibniz Universitat Hannover; AlemaniaFil: Witt, Martin. Leibniz Universitat Hannover; AlemaniaFil: Zorn, Holger. Leibniz Universitat Hannover; AlemaniaFil: Omarini, Alejandra Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias de la Tierra y Ambientales de La Pampa. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Instituto de Ciencias de la Tierra y Ambientales de La Pampa; ArgentinaFil: Ersoy, Franziska. Leibniz Universitat Hannover; AlemaniaMolecular Diversity Preservation International2021-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/183442Krahe, NinaKatharina; Berger, Ralf G.; Witt, Martin; Zorn, Holger; Omarini, Alejandra Beatriz; et al.; Monokaryotic pleurotus sapidus strains with intraspecific variability of an alkene cleaving dyp-type peroxidase activity as a result of gene mutation and differential gene expression; Molecular Diversity Preservation International; International Journal of Molecular Sciences; 22; 3; 1-2021; 1-241422-0067CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1422-0067/22/3/1363info:eu-repo/semantics/altIdentifier/doi/10.3390/ijms22031363info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:48:20Zoai:ri.conicet.gov.ar:11336/183442instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:48:20.787CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Monokaryotic pleurotus sapidus strains with intraspecific variability of an alkene cleaving dyp-type peroxidase activity as a result of gene mutation and differential gene expression
title Monokaryotic pleurotus sapidus strains with intraspecific variability of an alkene cleaving dyp-type peroxidase activity as a result of gene mutation and differential gene expression
spellingShingle Monokaryotic pleurotus sapidus strains with intraspecific variability of an alkene cleaving dyp-type peroxidase activity as a result of gene mutation and differential gene expression
Krahe, NinaKatharina
ALKENE CLEAVAGE
BASIDIOMYCOTA
BIOCATALYSIS
DIKARYON
DYE-DECOLORIZING PEROXIDASE (DYP)
GENE EXPRESSION
GENE MUTATION
INTRASPECIFIC VARIABILITY
MONOKARYON
PLEUROTUS SAPIDUS
title_short Monokaryotic pleurotus sapidus strains with intraspecific variability of an alkene cleaving dyp-type peroxidase activity as a result of gene mutation and differential gene expression
title_full Monokaryotic pleurotus sapidus strains with intraspecific variability of an alkene cleaving dyp-type peroxidase activity as a result of gene mutation and differential gene expression
title_fullStr Monokaryotic pleurotus sapidus strains with intraspecific variability of an alkene cleaving dyp-type peroxidase activity as a result of gene mutation and differential gene expression
title_full_unstemmed Monokaryotic pleurotus sapidus strains with intraspecific variability of an alkene cleaving dyp-type peroxidase activity as a result of gene mutation and differential gene expression
title_sort Monokaryotic pleurotus sapidus strains with intraspecific variability of an alkene cleaving dyp-type peroxidase activity as a result of gene mutation and differential gene expression
dc.creator.none.fl_str_mv Krahe, NinaKatharina
Berger, Ralf G.
Witt, Martin
Zorn, Holger
Omarini, Alejandra Beatriz
Ersoy, Franziska
author Krahe, NinaKatharina
author_facet Krahe, NinaKatharina
Berger, Ralf G.
Witt, Martin
Zorn, Holger
Omarini, Alejandra Beatriz
Ersoy, Franziska
author_role author
author2 Berger, Ralf G.
Witt, Martin
Zorn, Holger
Omarini, Alejandra Beatriz
Ersoy, Franziska
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv ALKENE CLEAVAGE
BASIDIOMYCOTA
BIOCATALYSIS
DIKARYON
DYE-DECOLORIZING PEROXIDASE (DYP)
GENE EXPRESSION
GENE MUTATION
INTRASPECIFIC VARIABILITY
MONOKARYON
PLEUROTUS SAPIDUS
topic ALKENE CLEAVAGE
BASIDIOMYCOTA
BIOCATALYSIS
DIKARYON
DYE-DECOLORIZING PEROXIDASE (DYP)
GENE EXPRESSION
GENE MUTATION
INTRASPECIFIC VARIABILITY
MONOKARYON
PLEUROTUS SAPIDUS
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The basidiomycete Pleurotus sapidus produced a dye-decolorizing peroxidase (PsaPOX) with alkene cleavage activity, implying potential as a biocatalyst for the fragrance and flavor industry. To increase the activity, a daughter-generation of 101 basidiospore-derived monokaryons (MK) was used. After a pre-selection according to the growth rate, the activity analysis revealed a stable intraspecific variability of the strains regarding peroxidase and alkene cleavage activity of PsaPOX. Ten monokaryons reached activities up to 2.6-fold higher than the dikaryon, with MK16 showing the highest activity. Analysis of the PsaPOX gene identified three different enzyme variants. These were co-responsible for the observed differences in activities between strains as verified by heterologous expression in Komagataella phaffii. The mutation S371H in enzyme variant PsaPOX_high caused an activity increase alongside a higher protein stability, while the eleven mutations in variant PsaPOX_low resulted in an activity decrease, which was partially based on a shift of the pH optimum from 3.5 to 3.0. Transcriptional analysis revealed the increased expression of PsaPOX in MK16 as reason for the higher PsaPOX activity in comparison to other strains producing the same PsaPOX variant. Thus, different expression profiles, as well as enzyme variants, were identified as crucial factors for the intraspecific variability of the PsaPOX activity in the monokaryons.
Fil: Krahe, NinaKatharina. Leibniz Universitat Hannover; Alemania
Fil: Berger, Ralf G.. Leibniz Universitat Hannover; Alemania
Fil: Witt, Martin. Leibniz Universitat Hannover; Alemania
Fil: Zorn, Holger. Leibniz Universitat Hannover; Alemania
Fil: Omarini, Alejandra Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias de la Tierra y Ambientales de La Pampa. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Instituto de Ciencias de la Tierra y Ambientales de La Pampa; Argentina
Fil: Ersoy, Franziska. Leibniz Universitat Hannover; Alemania
description The basidiomycete Pleurotus sapidus produced a dye-decolorizing peroxidase (PsaPOX) with alkene cleavage activity, implying potential as a biocatalyst for the fragrance and flavor industry. To increase the activity, a daughter-generation of 101 basidiospore-derived monokaryons (MK) was used. After a pre-selection according to the growth rate, the activity analysis revealed a stable intraspecific variability of the strains regarding peroxidase and alkene cleavage activity of PsaPOX. Ten monokaryons reached activities up to 2.6-fold higher than the dikaryon, with MK16 showing the highest activity. Analysis of the PsaPOX gene identified three different enzyme variants. These were co-responsible for the observed differences in activities between strains as verified by heterologous expression in Komagataella phaffii. The mutation S371H in enzyme variant PsaPOX_high caused an activity increase alongside a higher protein stability, while the eleven mutations in variant PsaPOX_low resulted in an activity decrease, which was partially based on a shift of the pH optimum from 3.5 to 3.0. Transcriptional analysis revealed the increased expression of PsaPOX in MK16 as reason for the higher PsaPOX activity in comparison to other strains producing the same PsaPOX variant. Thus, different expression profiles, as well as enzyme variants, were identified as crucial factors for the intraspecific variability of the PsaPOX activity in the monokaryons.
publishDate 2021
dc.date.none.fl_str_mv 2021-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/183442
Krahe, NinaKatharina; Berger, Ralf G.; Witt, Martin; Zorn, Holger; Omarini, Alejandra Beatriz; et al.; Monokaryotic pleurotus sapidus strains with intraspecific variability of an alkene cleaving dyp-type peroxidase activity as a result of gene mutation and differential gene expression; Molecular Diversity Preservation International; International Journal of Molecular Sciences; 22; 3; 1-2021; 1-24
1422-0067
CONICET Digital
CONICET
url http://hdl.handle.net/11336/183442
identifier_str_mv Krahe, NinaKatharina; Berger, Ralf G.; Witt, Martin; Zorn, Holger; Omarini, Alejandra Beatriz; et al.; Monokaryotic pleurotus sapidus strains with intraspecific variability of an alkene cleaving dyp-type peroxidase activity as a result of gene mutation and differential gene expression; Molecular Diversity Preservation International; International Journal of Molecular Sciences; 22; 3; 1-2021; 1-24
1422-0067
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1422-0067/22/3/1363
info:eu-repo/semantics/altIdentifier/doi/10.3390/ijms22031363
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Molecular Diversity Preservation International
publisher.none.fl_str_mv Molecular Diversity Preservation International
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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