A small fraction of dermatan sulfate with significantly increased anticoagulant activity was selected by interaction with the first complement protein

Autores
Calabrese, Graciela Cristina; Alberto, Maria Fabiana; Tubio, Maria Rosario; Marani, Mariela Mirta; Fernández De Recondo, Marta E.; Lazzari, María Ángela; Recondo, Eduardo F.
Año de publicación
2004
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Dermatan sulfate (DS) is a member of the family of structurally complex, sulfated, linear heteropolysaccharides called glycosaminoglycans (GAGs). It has a similar structure to heparin and heparan sulfate (HS), but with acetylgalactosamine replacing glucosamine, and the uronic acid moiety, mainly iduronic, joined 1→3 to the hexosamine. We are studying the relationships between structure and activities of dermatan sulfate, in particular those associated with the thrombin inhibition mediated by heparin cofactor II (HCII). As we have demonstrated with heparin, a small fraction of dermatan sulfate was isolated by precipitation with the first component of the complement system, under very specific conditions of low ionic strength, and the presence of calcium ions. The sulfate content and the anticoagulant activity of the dermatan sulfate fraction isolated in the precipitate were three and four times greater respectively than the starting material. Our in vivo studies showed that this fraction has threefold higher thrombolytic activity than the DS. All these results suggest that this fraction could be used as a therapeutic agent for thrombi dissolution. © 2004 Elsevier Ltd. All rights reserved.
Fil: Calabrese, Graciela Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas; Argentina
Fil: Alberto, Maria Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Tubio, Maria Rosario. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas; Argentina
Fil: Marani, Mariela Mirta. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas; Argentina
Fil: Fernández De Recondo, Marta E.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas; Argentina
Fil: Lazzari, María Ángela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Recondo, Eduardo F.. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Materia
Antithrombin
At
C1
Complement System
Con A
Concanavalin A
Dermatan Sulfate
Ds
Free Dermatan Sulfate
Free Ds
Gag
Glycosaminoglycan
Heparin Cofactor Ii
Hs
Molecular Interactions
The First Protein Complex Of the Complement System
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/66438
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/66438
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A small fraction of dermatan sulfate with significantly increased anticoagulant activity was selected by interaction with the first complement proteinCalabrese, Graciela CristinaAlberto, Maria FabianaTubio, Maria RosarioMarani, Mariela MirtaFernández De Recondo, Marta E.Lazzari, María ÁngelaRecondo, Eduardo F.AntithrombinAtC1Complement SystemCon AConcanavalin ADermatan SulfateDsFree Dermatan SulfateFree DsGagGlycosaminoglycanHeparin Cofactor IiHsMolecular InteractionsThe First Protein Complex Of the Complement Systemhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Dermatan sulfate (DS) is a member of the family of structurally complex, sulfated, linear heteropolysaccharides called glycosaminoglycans (GAGs). It has a similar structure to heparin and heparan sulfate (HS), but with acetylgalactosamine replacing glucosamine, and the uronic acid moiety, mainly iduronic, joined 1→3 to the hexosamine. We are studying the relationships between structure and activities of dermatan sulfate, in particular those associated with the thrombin inhibition mediated by heparin cofactor II (HCII). As we have demonstrated with heparin, a small fraction of dermatan sulfate was isolated by precipitation with the first component of the complement system, under very specific conditions of low ionic strength, and the presence of calcium ions. The sulfate content and the anticoagulant activity of the dermatan sulfate fraction isolated in the precipitate were three and four times greater respectively than the starting material. Our in vivo studies showed that this fraction has threefold higher thrombolytic activity than the DS. All these results suggest that this fraction could be used as a therapeutic agent for thrombi dissolution. © 2004 Elsevier Ltd. All rights reserved.Fil: Calabrese, Graciela Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas; ArgentinaFil: Alberto, Maria Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Tubio, Maria Rosario. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas; ArgentinaFil: Marani, Mariela Mirta. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas; ArgentinaFil: Fernández De Recondo, Marta E.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas; ArgentinaFil: Lazzari, María Ángela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Recondo, Eduardo F.. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; ArgentinaPergamon-Elsevier Science Ltd2004-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/66438Calabrese, Graciela Cristina; Alberto, Maria Fabiana; Tubio, Maria Rosario; Marani, Mariela Mirta; Fernández De Recondo, Marta E.; et al.; A small fraction of dermatan sulfate with significantly increased anticoagulant activity was selected by interaction with the first complement protein; Pergamon-Elsevier Science Ltd; Thrombosis Research; 113; 3-4; 1-2004; 243-2500049-3848CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.thromres.2004.01.014info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0049384804000143info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:06:41Zoai:ri.conicet.gov.ar:11336/66438instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:06:41.656CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A small fraction of dermatan sulfate with significantly increased anticoagulant activity was selected by interaction with the first complement protein
title A small fraction of dermatan sulfate with significantly increased anticoagulant activity was selected by interaction with the first complement protein
spellingShingle A small fraction of dermatan sulfate with significantly increased anticoagulant activity was selected by interaction with the first complement protein
Calabrese, Graciela Cristina
Antithrombin
At
C1
Complement System
Con A
Concanavalin A
Dermatan Sulfate
Ds
Free Dermatan Sulfate
Free Ds
Gag
Glycosaminoglycan
Heparin Cofactor Ii
Hs
Molecular Interactions
The First Protein Complex Of the Complement System
title_short A small fraction of dermatan sulfate with significantly increased anticoagulant activity was selected by interaction with the first complement protein
title_full A small fraction of dermatan sulfate with significantly increased anticoagulant activity was selected by interaction with the first complement protein
title_fullStr A small fraction of dermatan sulfate with significantly increased anticoagulant activity was selected by interaction with the first complement protein
title_full_unstemmed A small fraction of dermatan sulfate with significantly increased anticoagulant activity was selected by interaction with the first complement protein
title_sort A small fraction of dermatan sulfate with significantly increased anticoagulant activity was selected by interaction with the first complement protein
dc.creator.none.fl_str_mv Calabrese, Graciela Cristina
Alberto, Maria Fabiana
Tubio, Maria Rosario
Marani, Mariela Mirta
Fernández De Recondo, Marta E.
Lazzari, María Ángela
Recondo, Eduardo F.
author Calabrese, Graciela Cristina
author_facet Calabrese, Graciela Cristina
Alberto, Maria Fabiana
Tubio, Maria Rosario
Marani, Mariela Mirta
Fernández De Recondo, Marta E.
Lazzari, María Ángela
Recondo, Eduardo F.
author_role author
author2 Alberto, Maria Fabiana
Tubio, Maria Rosario
Marani, Mariela Mirta
Fernández De Recondo, Marta E.
Lazzari, María Ángela
Recondo, Eduardo F.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Antithrombin
At
C1
Complement System
Con A
Concanavalin A
Dermatan Sulfate
Ds
Free Dermatan Sulfate
Free Ds
Gag
Glycosaminoglycan
Heparin Cofactor Ii
Hs
Molecular Interactions
The First Protein Complex Of the Complement System
topic Antithrombin
At
C1
Complement System
Con A
Concanavalin A
Dermatan Sulfate
Ds
Free Dermatan Sulfate
Free Ds
Gag
Glycosaminoglycan
Heparin Cofactor Ii
Hs
Molecular Interactions
The First Protein Complex Of the Complement System
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Dermatan sulfate (DS) is a member of the family of structurally complex, sulfated, linear heteropolysaccharides called glycosaminoglycans (GAGs). It has a similar structure to heparin and heparan sulfate (HS), but with acetylgalactosamine replacing glucosamine, and the uronic acid moiety, mainly iduronic, joined 1→3 to the hexosamine. We are studying the relationships between structure and activities of dermatan sulfate, in particular those associated with the thrombin inhibition mediated by heparin cofactor II (HCII). As we have demonstrated with heparin, a small fraction of dermatan sulfate was isolated by precipitation with the first component of the complement system, under very specific conditions of low ionic strength, and the presence of calcium ions. The sulfate content and the anticoagulant activity of the dermatan sulfate fraction isolated in the precipitate were three and four times greater respectively than the starting material. Our in vivo studies showed that this fraction has threefold higher thrombolytic activity than the DS. All these results suggest that this fraction could be used as a therapeutic agent for thrombi dissolution. © 2004 Elsevier Ltd. All rights reserved.
Fil: Calabrese, Graciela Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas; Argentina
Fil: Alberto, Maria Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Tubio, Maria Rosario. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas; Argentina
Fil: Marani, Mariela Mirta. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas; Argentina
Fil: Fernández De Recondo, Marta E.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas; Argentina
Fil: Lazzari, María Ángela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Recondo, Eduardo F.. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
description Dermatan sulfate (DS) is a member of the family of structurally complex, sulfated, linear heteropolysaccharides called glycosaminoglycans (GAGs). It has a similar structure to heparin and heparan sulfate (HS), but with acetylgalactosamine replacing glucosamine, and the uronic acid moiety, mainly iduronic, joined 1→3 to the hexosamine. We are studying the relationships between structure and activities of dermatan sulfate, in particular those associated with the thrombin inhibition mediated by heparin cofactor II (HCII). As we have demonstrated with heparin, a small fraction of dermatan sulfate was isolated by precipitation with the first component of the complement system, under very specific conditions of low ionic strength, and the presence of calcium ions. The sulfate content and the anticoagulant activity of the dermatan sulfate fraction isolated in the precipitate were three and four times greater respectively than the starting material. Our in vivo studies showed that this fraction has threefold higher thrombolytic activity than the DS. All these results suggest that this fraction could be used as a therapeutic agent for thrombi dissolution. © 2004 Elsevier Ltd. All rights reserved.
publishDate 2004
dc.date.none.fl_str_mv 2004-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/66438
Calabrese, Graciela Cristina; Alberto, Maria Fabiana; Tubio, Maria Rosario; Marani, Mariela Mirta; Fernández De Recondo, Marta E.; et al.; A small fraction of dermatan sulfate with significantly increased anticoagulant activity was selected by interaction with the first complement protein; Pergamon-Elsevier Science Ltd; Thrombosis Research; 113; 3-4; 1-2004; 243-250
0049-3848
CONICET Digital
CONICET
url http://hdl.handle.net/11336/66438
identifier_str_mv Calabrese, Graciela Cristina; Alberto, Maria Fabiana; Tubio, Maria Rosario; Marani, Mariela Mirta; Fernández De Recondo, Marta E.; et al.; A small fraction of dermatan sulfate with significantly increased anticoagulant activity was selected by interaction with the first complement protein; Pergamon-Elsevier Science Ltd; Thrombosis Research; 113; 3-4; 1-2004; 243-250
0049-3848
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.thromres.2004.01.014
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0049384804000143
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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