Reorganization of the flagellum scaffolding induces a sperm standstill during fertilization

Autores
Jabloñski, Martina; Luque, Guillermina Maria; Gómez Elías, Matías Daniel; Sanchez Cardenas, Claudia; Xu, Xinran; de La Vega Beltran, Jose L; Corkidi, Gabriel; Linares, Alejandro; Abonza, Victor; Arenas Hernandez, Aquetzalli; Ramos Godinez, María D. P.; López Saavedra, Alejandro; Krapf, Dario; Krapf, Diego; Darszon, Alberto; Guerrero, Adán; Buffone, Mariano Gabriel
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Mammalian sperm delve into the female reproductive tract to fertilize the female gamete. The available information about how sperm regulate their motility during the final journey to the fertilization site is extremely limited. In this work, we investigated the structural and functional changes in the sperm flagellum after acrosomal exocytosis (AE) and during the interaction with the eggs. The evidence demonstrates that the double helix actin network surrounding the mitochondrial sheath of the midpiece undergoes structural changes prior to the motility cessation. This structural modification is accompanied by a decrease in diameter of the midpiece and is driven by intracellular calcium changes that occur concomitant with a reorganization of the actin helicoidal cortex. Midpiece contraction occurs in a subset of cells that undergo AE, and live-cell imaging during in vitro fertilization showed that the midpiece contraction is required for motility cessation after fusion is initiated. These findings provide the first evidence of the F-actin network´s role in regulating sperm motility, adapting its function to meet specific cellular requirements during fertilization, and highlighting the broader significance of understanding sperm motility.
Fil: Jabloñski, Martina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Luque, Guillermina Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Gómez Elías, Matías Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Sanchez Cardenas, Claudia. Universidad Nacional Autónoma de México. Instituto de Biotecnología; México
Fil: Xu, Xinran. State University of Colorado - Fort Collins; Estados Unidos
Fil: de La Vega Beltran, Jose L. Universidad Nacional Autónoma de México. Instituto de Biotecnología; México
Fil: Corkidi, Gabriel. Universidad Nacional Autónoma de México. Instituto de Biotecnología; México
Fil: Linares, Alejandro. Universidad Nacional Autónoma de México. Instituto de Biotecnología; México
Fil: Abonza, Victor. Universidad Nacional Autónoma de México. Instituto de Biotecnología; México
Fil: Arenas Hernandez, Aquetzalli. Instituto Nacional de Cancerología; México
Fil: Ramos Godinez, María D. P.. Instituto Nacional de Cancerología; México
Fil: López Saavedra, Alejandro. Instituto Nacional de Cancerología; México. Instituto Tecnológico de Monterrey; México
Fil: Krapf, Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Krapf, Diego. State University of Colorado - Fort Collins; Estados Unidos
Fil: Darszon, Alberto. Universidad Nacional Autónoma de México. Instituto de Biotecnología; México
Fil: Guerrero, Adán. Universidad Nacional Autónoma de México. Instituto de Biotecnología; México
Fil: Buffone, Mariano Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Materia
ACTIN
CALCIUM
DEVELOPMENTAL BIOLOGY
FUSION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/266858
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/266858
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Reorganization of the flagellum scaffolding induces a sperm standstill during fertilizationJabloñski, MartinaLuque, Guillermina MariaGómez Elías, Matías DanielSanchez Cardenas, ClaudiaXu, Xinrande La Vega Beltran, Jose LCorkidi, GabrielLinares, AlejandroAbonza, VictorArenas Hernandez, AquetzalliRamos Godinez, María D. P.López Saavedra, AlejandroKrapf, DarioKrapf, DiegoDarszon, AlbertoGuerrero, AdánBuffone, Mariano GabrielACTINCALCIUMDEVELOPMENTAL BIOLOGYFUSIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Mammalian sperm delve into the female reproductive tract to fertilize the female gamete. The available information about how sperm regulate their motility during the final journey to the fertilization site is extremely limited. In this work, we investigated the structural and functional changes in the sperm flagellum after acrosomal exocytosis (AE) and during the interaction with the eggs. The evidence demonstrates that the double helix actin network surrounding the mitochondrial sheath of the midpiece undergoes structural changes prior to the motility cessation. This structural modification is accompanied by a decrease in diameter of the midpiece and is driven by intracellular calcium changes that occur concomitant with a reorganization of the actin helicoidal cortex. Midpiece contraction occurs in a subset of cells that undergo AE, and live-cell imaging during in vitro fertilization showed that the midpiece contraction is required for motility cessation after fusion is initiated. These findings provide the first evidence of the F-actin network´s role in regulating sperm motility, adapting its function to meet specific cellular requirements during fertilization, and highlighting the broader significance of understanding sperm motility.Fil: Jabloñski, Martina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Luque, Guillermina Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Gómez Elías, Matías Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Sanchez Cardenas, Claudia. Universidad Nacional Autónoma de México. Instituto de Biotecnología; MéxicoFil: Xu, Xinran. State University of Colorado - Fort Collins; Estados UnidosFil: de La Vega Beltran, Jose L. Universidad Nacional Autónoma de México. Instituto de Biotecnología; MéxicoFil: Corkidi, Gabriel. Universidad Nacional Autónoma de México. Instituto de Biotecnología; MéxicoFil: Linares, Alejandro. Universidad Nacional Autónoma de México. Instituto de Biotecnología; MéxicoFil: Abonza, Victor. Universidad Nacional Autónoma de México. Instituto de Biotecnología; MéxicoFil: Arenas Hernandez, Aquetzalli. Instituto Nacional de Cancerología; MéxicoFil: Ramos Godinez, María D. P.. Instituto Nacional de Cancerología; MéxicoFil: López Saavedra, Alejandro. Instituto Nacional de Cancerología; México. Instituto Tecnológico de Monterrey; MéxicoFil: Krapf, Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Krapf, Diego. State University of Colorado - Fort Collins; Estados UnidosFil: Darszon, Alberto. Universidad Nacional Autónoma de México. Instituto de Biotecnología; MéxicoFil: Guerrero, Adán. Universidad Nacional Autónoma de México. Instituto de Biotecnología; MéxicoFil: Buffone, Mariano Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaeLife Sciences Publications2024-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/266858Jabloñski, Martina; Luque, Guillermina Maria; Gómez Elías, Matías Daniel; Sanchez Cardenas, Claudia; Xu, Xinran; et al.; Reorganization of the flagellum scaffolding induces a sperm standstill during fertilization; eLife Sciences Publications; eLife; 13; 11-2024; 1-312050-084XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://elifesciences.org/articles/93792info:eu-repo/semantics/altIdentifier/doi/10.7554/eLife.93792info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:24:33Zoai:ri.conicet.gov.ar:11336/266858instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:24:33.458CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Reorganization of the flagellum scaffolding induces a sperm standstill during fertilization
title Reorganization of the flagellum scaffolding induces a sperm standstill during fertilization
spellingShingle Reorganization of the flagellum scaffolding induces a sperm standstill during fertilization
Jabloñski, Martina
ACTIN
CALCIUM
DEVELOPMENTAL BIOLOGY
FUSION
title_short Reorganization of the flagellum scaffolding induces a sperm standstill during fertilization
title_full Reorganization of the flagellum scaffolding induces a sperm standstill during fertilization
title_fullStr Reorganization of the flagellum scaffolding induces a sperm standstill during fertilization
title_full_unstemmed Reorganization of the flagellum scaffolding induces a sperm standstill during fertilization
title_sort Reorganization of the flagellum scaffolding induces a sperm standstill during fertilization
dc.creator.none.fl_str_mv Jabloñski, Martina
Luque, Guillermina Maria
Gómez Elías, Matías Daniel
Sanchez Cardenas, Claudia
Xu, Xinran
de La Vega Beltran, Jose L
Corkidi, Gabriel
Linares, Alejandro
Abonza, Victor
Arenas Hernandez, Aquetzalli
Ramos Godinez, María D. P.
López Saavedra, Alejandro
Krapf, Dario
Krapf, Diego
Darszon, Alberto
Guerrero, Adán
Buffone, Mariano Gabriel
author Jabloñski, Martina
author_facet Jabloñski, Martina
Luque, Guillermina Maria
Gómez Elías, Matías Daniel
Sanchez Cardenas, Claudia
Xu, Xinran
de La Vega Beltran, Jose L
Corkidi, Gabriel
Linares, Alejandro
Abonza, Victor
Arenas Hernandez, Aquetzalli
Ramos Godinez, María D. P.
López Saavedra, Alejandro
Krapf, Dario
Krapf, Diego
Darszon, Alberto
Guerrero, Adán
Buffone, Mariano Gabriel
author_role author
author2 Luque, Guillermina Maria
Gómez Elías, Matías Daniel
Sanchez Cardenas, Claudia
Xu, Xinran
de La Vega Beltran, Jose L
Corkidi, Gabriel
Linares, Alejandro
Abonza, Victor
Arenas Hernandez, Aquetzalli
Ramos Godinez, María D. P.
López Saavedra, Alejandro
Krapf, Dario
Krapf, Diego
Darszon, Alberto
Guerrero, Adán
Buffone, Mariano Gabriel
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ACTIN
CALCIUM
DEVELOPMENTAL BIOLOGY
FUSION
topic ACTIN
CALCIUM
DEVELOPMENTAL BIOLOGY
FUSION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Mammalian sperm delve into the female reproductive tract to fertilize the female gamete. The available information about how sperm regulate their motility during the final journey to the fertilization site is extremely limited. In this work, we investigated the structural and functional changes in the sperm flagellum after acrosomal exocytosis (AE) and during the interaction with the eggs. The evidence demonstrates that the double helix actin network surrounding the mitochondrial sheath of the midpiece undergoes structural changes prior to the motility cessation. This structural modification is accompanied by a decrease in diameter of the midpiece and is driven by intracellular calcium changes that occur concomitant with a reorganization of the actin helicoidal cortex. Midpiece contraction occurs in a subset of cells that undergo AE, and live-cell imaging during in vitro fertilization showed that the midpiece contraction is required for motility cessation after fusion is initiated. These findings provide the first evidence of the F-actin network´s role in regulating sperm motility, adapting its function to meet specific cellular requirements during fertilization, and highlighting the broader significance of understanding sperm motility.
Fil: Jabloñski, Martina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Luque, Guillermina Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Gómez Elías, Matías Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Sanchez Cardenas, Claudia. Universidad Nacional Autónoma de México. Instituto de Biotecnología; México
Fil: Xu, Xinran. State University of Colorado - Fort Collins; Estados Unidos
Fil: de La Vega Beltran, Jose L. Universidad Nacional Autónoma de México. Instituto de Biotecnología; México
Fil: Corkidi, Gabriel. Universidad Nacional Autónoma de México. Instituto de Biotecnología; México
Fil: Linares, Alejandro. Universidad Nacional Autónoma de México. Instituto de Biotecnología; México
Fil: Abonza, Victor. Universidad Nacional Autónoma de México. Instituto de Biotecnología; México
Fil: Arenas Hernandez, Aquetzalli. Instituto Nacional de Cancerología; México
Fil: Ramos Godinez, María D. P.. Instituto Nacional de Cancerología; México
Fil: López Saavedra, Alejandro. Instituto Nacional de Cancerología; México. Instituto Tecnológico de Monterrey; México
Fil: Krapf, Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Krapf, Diego. State University of Colorado - Fort Collins; Estados Unidos
Fil: Darszon, Alberto. Universidad Nacional Autónoma de México. Instituto de Biotecnología; México
Fil: Guerrero, Adán. Universidad Nacional Autónoma de México. Instituto de Biotecnología; México
Fil: Buffone, Mariano Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
description Mammalian sperm delve into the female reproductive tract to fertilize the female gamete. The available information about how sperm regulate their motility during the final journey to the fertilization site is extremely limited. In this work, we investigated the structural and functional changes in the sperm flagellum after acrosomal exocytosis (AE) and during the interaction with the eggs. The evidence demonstrates that the double helix actin network surrounding the mitochondrial sheath of the midpiece undergoes structural changes prior to the motility cessation. This structural modification is accompanied by a decrease in diameter of the midpiece and is driven by intracellular calcium changes that occur concomitant with a reorganization of the actin helicoidal cortex. Midpiece contraction occurs in a subset of cells that undergo AE, and live-cell imaging during in vitro fertilization showed that the midpiece contraction is required for motility cessation after fusion is initiated. These findings provide the first evidence of the F-actin network´s role in regulating sperm motility, adapting its function to meet specific cellular requirements during fertilization, and highlighting the broader significance of understanding sperm motility.
publishDate 2024
dc.date.none.fl_str_mv 2024-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/266858
Jabloñski, Martina; Luque, Guillermina Maria; Gómez Elías, Matías Daniel; Sanchez Cardenas, Claudia; Xu, Xinran; et al.; Reorganization of the flagellum scaffolding induces a sperm standstill during fertilization; eLife Sciences Publications; eLife; 13; 11-2024; 1-31
2050-084X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/266858
identifier_str_mv Jabloñski, Martina; Luque, Guillermina Maria; Gómez Elías, Matías Daniel; Sanchez Cardenas, Claudia; Xu, Xinran; et al.; Reorganization of the flagellum scaffolding induces a sperm standstill during fertilization; eLife Sciences Publications; eLife; 13; 11-2024; 1-31
2050-084X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://elifesciences.org/articles/93792
info:eu-repo/semantics/altIdentifier/doi/10.7554/eLife.93792
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv eLife Sciences Publications
publisher.none.fl_str_mv eLife Sciences Publications
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.48226

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