The actin cytoskeleton of the mouse sperm flagellum is organized in a helical structure

Autores
Gervasi, Maria Gracia; Xu, Xinran; Carbajal Gonzalez, Blanca; Buffone, Mariano Gabriel; Visconti, Pablo E.; Krapf, Diego
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Conception in mammals is determined by the fusion of a sperm cell with an oocyte during fertilization. Motility is one of the features of sperm that allows them to succeed in fertilization, and their flagellum is essential for this function. Longitudinally, the flagellum can be divided into the midpiece, the principal piece and the end piece. A precise cytoskeletal architecture of the sperm tail is key for the acquisition of fertilization competence. It has been proposed that the actin cytoskeleton plays essential roles in the regulation of sperm motility; however, the actin organization in sperm remains elusive. In the present work, we show that there are different types of actin structures in the sperm tail by using three-dimensional stochastic optical reconstruction microscopy (STORM). In the principal piece, actin is radially distributed between the axoneme and the plasma membrane. The actin-associated proteins spectrin and adducin are also found in these structures. Strikingly, polymerized actin in the midpiece forms a double-helix that accompanies mitochondria. Our findings illustrate a novel specialized structure of actin filaments in a mammalian cell.
Fil: Gervasi, Maria Gracia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina. University of Massachussets; Estados Unidos
Fil: Xu, Xinran. State University of Colorado - Fort Collins; Estados Unidos
Fil: Carbajal Gonzalez, Blanca. Mount Holyoke College; Estados Unidos
Fil: Buffone, Mariano Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Visconti, Pablo E.. University of Massachussets; Estados Unidos
Fil: Krapf, Diego. State University of Colorado - Fort Collins; Estados Unidos
Materia
SPERM
ACTIN
MITOCHONDRIA
FERTILIZATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/90775
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The actin cytoskeleton of the mouse sperm flagellum is organized in a helical structureGervasi, Maria GraciaXu, XinranCarbajal Gonzalez, BlancaBuffone, Mariano GabrielVisconti, Pablo E.Krapf, DiegoSPERMACTINMITOCHONDRIAFERTILIZATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Conception in mammals is determined by the fusion of a sperm cell with an oocyte during fertilization. Motility is one of the features of sperm that allows them to succeed in fertilization, and their flagellum is essential for this function. Longitudinally, the flagellum can be divided into the midpiece, the principal piece and the end piece. A precise cytoskeletal architecture of the sperm tail is key for the acquisition of fertilization competence. It has been proposed that the actin cytoskeleton plays essential roles in the regulation of sperm motility; however, the actin organization in sperm remains elusive. In the present work, we show that there are different types of actin structures in the sperm tail by using three-dimensional stochastic optical reconstruction microscopy (STORM). In the principal piece, actin is radially distributed between the axoneme and the plasma membrane. The actin-associated proteins spectrin and adducin are also found in these structures. Strikingly, polymerized actin in the midpiece forms a double-helix that accompanies mitochondria. Our findings illustrate a novel specialized structure of actin filaments in a mammalian cell.Fil: Gervasi, Maria Gracia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina. University of Massachussets; Estados UnidosFil: Xu, Xinran. State University of Colorado - Fort Collins; Estados UnidosFil: Carbajal Gonzalez, Blanca. Mount Holyoke College; Estados UnidosFil: Buffone, Mariano Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Visconti, Pablo E.. University of Massachussets; Estados UnidosFil: Krapf, Diego. State University of Colorado - Fort Collins; Estados UnidosCompany of Biologists2018-06-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfhttp://hdl.handle.net/11336/90775Gervasi, Maria Gracia; Xu, Xinran; Carbajal Gonzalez, Blanca; Buffone, Mariano Gabriel; Visconti, Pablo E.; et al.; The actin cytoskeleton of the mouse sperm flagellum is organized in a helical structure; Company of Biologists; Journal of Cell Science; 131; 11; 11-6-2018; 215897-2159920021-9533CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://jcs.biologists.org/lookup/doi/10.1242/jcs.215897info:eu-repo/semantics/altIdentifier/doi/10.1242/jcs.215897info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:15:51Zoai:ri.conicet.gov.ar:11336/90775instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:15:51.399CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The actin cytoskeleton of the mouse sperm flagellum is organized in a helical structure
title The actin cytoskeleton of the mouse sperm flagellum is organized in a helical structure
spellingShingle The actin cytoskeleton of the mouse sperm flagellum is organized in a helical structure
Gervasi, Maria Gracia
SPERM
ACTIN
MITOCHONDRIA
FERTILIZATION
title_short The actin cytoskeleton of the mouse sperm flagellum is organized in a helical structure
title_full The actin cytoskeleton of the mouse sperm flagellum is organized in a helical structure
title_fullStr The actin cytoskeleton of the mouse sperm flagellum is organized in a helical structure
title_full_unstemmed The actin cytoskeleton of the mouse sperm flagellum is organized in a helical structure
title_sort The actin cytoskeleton of the mouse sperm flagellum is organized in a helical structure
dc.creator.none.fl_str_mv Gervasi, Maria Gracia
Xu, Xinran
Carbajal Gonzalez, Blanca
Buffone, Mariano Gabriel
Visconti, Pablo E.
Krapf, Diego
author Gervasi, Maria Gracia
author_facet Gervasi, Maria Gracia
Xu, Xinran
Carbajal Gonzalez, Blanca
Buffone, Mariano Gabriel
Visconti, Pablo E.
Krapf, Diego
author_role author
author2 Xu, Xinran
Carbajal Gonzalez, Blanca
Buffone, Mariano Gabriel
Visconti, Pablo E.
Krapf, Diego
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv SPERM
ACTIN
MITOCHONDRIA
FERTILIZATION
topic SPERM
ACTIN
MITOCHONDRIA
FERTILIZATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Conception in mammals is determined by the fusion of a sperm cell with an oocyte during fertilization. Motility is one of the features of sperm that allows them to succeed in fertilization, and their flagellum is essential for this function. Longitudinally, the flagellum can be divided into the midpiece, the principal piece and the end piece. A precise cytoskeletal architecture of the sperm tail is key for the acquisition of fertilization competence. It has been proposed that the actin cytoskeleton plays essential roles in the regulation of sperm motility; however, the actin organization in sperm remains elusive. In the present work, we show that there are different types of actin structures in the sperm tail by using three-dimensional stochastic optical reconstruction microscopy (STORM). In the principal piece, actin is radially distributed between the axoneme and the plasma membrane. The actin-associated proteins spectrin and adducin are also found in these structures. Strikingly, polymerized actin in the midpiece forms a double-helix that accompanies mitochondria. Our findings illustrate a novel specialized structure of actin filaments in a mammalian cell.
Fil: Gervasi, Maria Gracia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina. University of Massachussets; Estados Unidos
Fil: Xu, Xinran. State University of Colorado - Fort Collins; Estados Unidos
Fil: Carbajal Gonzalez, Blanca. Mount Holyoke College; Estados Unidos
Fil: Buffone, Mariano Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Visconti, Pablo E.. University of Massachussets; Estados Unidos
Fil: Krapf, Diego. State University of Colorado - Fort Collins; Estados Unidos
description Conception in mammals is determined by the fusion of a sperm cell with an oocyte during fertilization. Motility is one of the features of sperm that allows them to succeed in fertilization, and their flagellum is essential for this function. Longitudinally, the flagellum can be divided into the midpiece, the principal piece and the end piece. A precise cytoskeletal architecture of the sperm tail is key for the acquisition of fertilization competence. It has been proposed that the actin cytoskeleton plays essential roles in the regulation of sperm motility; however, the actin organization in sperm remains elusive. In the present work, we show that there are different types of actin structures in the sperm tail by using three-dimensional stochastic optical reconstruction microscopy (STORM). In the principal piece, actin is radially distributed between the axoneme and the plasma membrane. The actin-associated proteins spectrin and adducin are also found in these structures. Strikingly, polymerized actin in the midpiece forms a double-helix that accompanies mitochondria. Our findings illustrate a novel specialized structure of actin filaments in a mammalian cell.
publishDate 2018
dc.date.none.fl_str_mv 2018-06-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/90775
Gervasi, Maria Gracia; Xu, Xinran; Carbajal Gonzalez, Blanca; Buffone, Mariano Gabriel; Visconti, Pablo E.; et al.; The actin cytoskeleton of the mouse sperm flagellum is organized in a helical structure; Company of Biologists; Journal of Cell Science; 131; 11; 11-6-2018; 215897-215992
0021-9533
CONICET Digital
CONICET
url http://hdl.handle.net/11336/90775
identifier_str_mv Gervasi, Maria Gracia; Xu, Xinran; Carbajal Gonzalez, Blanca; Buffone, Mariano Gabriel; Visconti, Pablo E.; et al.; The actin cytoskeleton of the mouse sperm flagellum is organized in a helical structure; Company of Biologists; Journal of Cell Science; 131; 11; 11-6-2018; 215897-215992
0021-9533
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://jcs.biologists.org/lookup/doi/10.1242/jcs.215897
info:eu-repo/semantics/altIdentifier/doi/10.1242/jcs.215897
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/vnd.openxmlformats-officedocument.wordprocessingml.document
application/pdf
dc.publisher.none.fl_str_mv Company of Biologists
publisher.none.fl_str_mv Company of Biologists
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846781598150688768
score 12.982451

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