Fluorescent immunosensor using AP-SNs and QDs for quantitation of IgG anti-Toxocara canis

Autores
Medawar Aguilar, Victoria; Messina, Germán Alejandro; Fernández Baldo, Martín Alejandro; Raba, Julio; Pereira, Sirley Vanesa
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The objective of this work was to develop a microfluidic immunosensor for the quantitative determination of IgG antibodies to Toxocara canis (IgG anti-T.canis), causal agent of toxocariasis. This disease is caused by accidental ingestion of infective eggs that hatch into the first portion of the intestine. Subsequently, the juvenile stages are distributed throughout the body, generating symptoms from mild to severe manifestations. The possibility of early diagnosis is of great importance, allowing proper management and treatment of patients suffering from toxocariasis. IgG anti-T.canis antibodies detection was carried out using a non-competitive immunoassay, in which excretory-secretory antigens from T. canis second-stage larvae (TES) were covalently immobilized on 3-aminopropyl-functionalized silica-nanoparticles (AP-SNs). Antibodies present in serum samples immunologically reacted with TES and then were quantified by using a second antibody labeled with cadmium selenide zinc sulfide quantum dots (CdSe-ZnS QDs). The concentration of IgG anti-T. canis antibodies present in the serum sample was measured by LIF detector, using excitation lambda at 491 nm and emission at 540 nm. The total assay time was 30 minutes, having made LIF detection in less than 1 minute. The detection limit calculated for the proposed methodology was 0.12 ng mL-1 and the coefficients of intra- and inter-assay variation were less than 6%. The results show the usefulness of the developed immunosensor for the fast determination of IgG antibodies anti T. canis.
Fil: Medawar Aguilar, Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Messina, Germán Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Fernández Baldo, Martín Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Pereira, Sirley Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Materia
Toxocara
Microfluidic Immunosensor
Laser Induced Fluorescence
Quantumdot
Silica Particle
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/64763
Acceder
id CONICETDig_9ccf08decfabdedd91fbd85cc228f15f
oai_identifier_str oai:ri.conicet.gov.ar:11336/64763
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Fluorescent immunosensor using AP-SNs and QDs for quantitation of IgG anti-Toxocara canisMedawar Aguilar, VictoriaMessina, Germán AlejandroFernández Baldo, Martín AlejandroRaba, JulioPereira, Sirley VanesaToxocaraMicrofluidic ImmunosensorLaser Induced FluorescenceQuantumdotSilica Particlehttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The objective of this work was to develop a microfluidic immunosensor for the quantitative determination of IgG antibodies to Toxocara canis (IgG anti-T.canis), causal agent of toxocariasis. This disease is caused by accidental ingestion of infective eggs that hatch into the first portion of the intestine. Subsequently, the juvenile stages are distributed throughout the body, generating symptoms from mild to severe manifestations. The possibility of early diagnosis is of great importance, allowing proper management and treatment of patients suffering from toxocariasis. IgG anti-T.canis antibodies detection was carried out using a non-competitive immunoassay, in which excretory-secretory antigens from T. canis second-stage larvae (TES) were covalently immobilized on 3-aminopropyl-functionalized silica-nanoparticles (AP-SNs). Antibodies present in serum samples immunologically reacted with TES and then were quantified by using a second antibody labeled with cadmium selenide zinc sulfide quantum dots (CdSe-ZnS QDs). The concentration of IgG anti-T. canis antibodies present in the serum sample was measured by LIF detector, using excitation lambda at 491 nm and emission at 540 nm. The total assay time was 30 minutes, having made LIF detection in less than 1 minute. The detection limit calculated for the proposed methodology was 0.12 ng mL-1 and the coefficients of intra- and inter-assay variation were less than 6%. The results show the usefulness of the developed immunosensor for the fast determination of IgG antibodies anti T. canis.Fil: Medawar Aguilar, Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Messina, Germán Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Fernández Baldo, Martín Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Pereira, Sirley Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaElsevier Science2017-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/64763Medawar Aguilar, Victoria; Messina, Germán Alejandro; Fernández Baldo, Martín Alejandro; Raba, Julio; Pereira, Sirley Vanesa; Fluorescent immunosensor using AP-SNs and QDs for quantitation of IgG anti-Toxocara canis; Elsevier Science; Microchemical Journal; 130; 1-2017; 436-4410026-265XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.microc.2016.10.027info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:43:32Zoai:ri.conicet.gov.ar:11336/64763instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:43:32.955CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Fluorescent immunosensor using AP-SNs and QDs for quantitation of IgG anti-Toxocara canis
title Fluorescent immunosensor using AP-SNs and QDs for quantitation of IgG anti-Toxocara canis
spellingShingle Fluorescent immunosensor using AP-SNs and QDs for quantitation of IgG anti-Toxocara canis
Medawar Aguilar, Victoria
Toxocara
Microfluidic Immunosensor
Laser Induced Fluorescence
Quantumdot
Silica Particle
title_short Fluorescent immunosensor using AP-SNs and QDs for quantitation of IgG anti-Toxocara canis
title_full Fluorescent immunosensor using AP-SNs and QDs for quantitation of IgG anti-Toxocara canis
title_fullStr Fluorescent immunosensor using AP-SNs and QDs for quantitation of IgG anti-Toxocara canis
title_full_unstemmed Fluorescent immunosensor using AP-SNs and QDs for quantitation of IgG anti-Toxocara canis
title_sort Fluorescent immunosensor using AP-SNs and QDs for quantitation of IgG anti-Toxocara canis
dc.creator.none.fl_str_mv Medawar Aguilar, Victoria
Messina, Germán Alejandro
Fernández Baldo, Martín Alejandro
Raba, Julio
Pereira, Sirley Vanesa
author Medawar Aguilar, Victoria
author_facet Medawar Aguilar, Victoria
Messina, Germán Alejandro
Fernández Baldo, Martín Alejandro
Raba, Julio
Pereira, Sirley Vanesa
author_role author
author2 Messina, Germán Alejandro
Fernández Baldo, Martín Alejandro
Raba, Julio
Pereira, Sirley Vanesa
author2_role author
author
author
author
dc.subject.none.fl_str_mv Toxocara
Microfluidic Immunosensor
Laser Induced Fluorescence
Quantumdot
Silica Particle
topic Toxocara
Microfluidic Immunosensor
Laser Induced Fluorescence
Quantumdot
Silica Particle
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The objective of this work was to develop a microfluidic immunosensor for the quantitative determination of IgG antibodies to Toxocara canis (IgG anti-T.canis), causal agent of toxocariasis. This disease is caused by accidental ingestion of infective eggs that hatch into the first portion of the intestine. Subsequently, the juvenile stages are distributed throughout the body, generating symptoms from mild to severe manifestations. The possibility of early diagnosis is of great importance, allowing proper management and treatment of patients suffering from toxocariasis. IgG anti-T.canis antibodies detection was carried out using a non-competitive immunoassay, in which excretory-secretory antigens from T. canis second-stage larvae (TES) were covalently immobilized on 3-aminopropyl-functionalized silica-nanoparticles (AP-SNs). Antibodies present in serum samples immunologically reacted with TES and then were quantified by using a second antibody labeled with cadmium selenide zinc sulfide quantum dots (CdSe-ZnS QDs). The concentration of IgG anti-T. canis antibodies present in the serum sample was measured by LIF detector, using excitation lambda at 491 nm and emission at 540 nm. The total assay time was 30 minutes, having made LIF detection in less than 1 minute. The detection limit calculated for the proposed methodology was 0.12 ng mL-1 and the coefficients of intra- and inter-assay variation were less than 6%. The results show the usefulness of the developed immunosensor for the fast determination of IgG antibodies anti T. canis.
Fil: Medawar Aguilar, Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Messina, Germán Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Fernández Baldo, Martín Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Pereira, Sirley Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
description The objective of this work was to develop a microfluidic immunosensor for the quantitative determination of IgG antibodies to Toxocara canis (IgG anti-T.canis), causal agent of toxocariasis. This disease is caused by accidental ingestion of infective eggs that hatch into the first portion of the intestine. Subsequently, the juvenile stages are distributed throughout the body, generating symptoms from mild to severe manifestations. The possibility of early diagnosis is of great importance, allowing proper management and treatment of patients suffering from toxocariasis. IgG anti-T.canis antibodies detection was carried out using a non-competitive immunoassay, in which excretory-secretory antigens from T. canis second-stage larvae (TES) were covalently immobilized on 3-aminopropyl-functionalized silica-nanoparticles (AP-SNs). Antibodies present in serum samples immunologically reacted with TES and then were quantified by using a second antibody labeled with cadmium selenide zinc sulfide quantum dots (CdSe-ZnS QDs). The concentration of IgG anti-T. canis antibodies present in the serum sample was measured by LIF detector, using excitation lambda at 491 nm and emission at 540 nm. The total assay time was 30 minutes, having made LIF detection in less than 1 minute. The detection limit calculated for the proposed methodology was 0.12 ng mL-1 and the coefficients of intra- and inter-assay variation were less than 6%. The results show the usefulness of the developed immunosensor for the fast determination of IgG antibodies anti T. canis.
publishDate 2017
dc.date.none.fl_str_mv 2017-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/64763
Medawar Aguilar, Victoria; Messina, Germán Alejandro; Fernández Baldo, Martín Alejandro; Raba, Julio; Pereira, Sirley Vanesa; Fluorescent immunosensor using AP-SNs and QDs for quantitation of IgG anti-Toxocara canis; Elsevier Science; Microchemical Journal; 130; 1-2017; 436-441
0026-265X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/64763
identifier_str_mv Medawar Aguilar, Victoria; Messina, Germán Alejandro; Fernández Baldo, Martín Alejandro; Raba, Julio; Pereira, Sirley Vanesa; Fluorescent immunosensor using AP-SNs and QDs for quantitation of IgG anti-Toxocara canis; Elsevier Science; Microchemical Journal; 130; 1-2017; 436-441
0026-265X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.microc.2016.10.027
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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