Laser-induced fluorescence integrated in a microfluidic immunosensor for quantification of human serum IgG antibodies to Helicobacter pylori

Autores
Seia, Marco Abel; Pereira, Sirley Vanesa; Fontán, Carlos A.; de Vito, Irma Esther; Messina, Germán Alejandro; Raba, Julio
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A portable immunosensor coupled to laser-induced fluorescence (LIF) detection system has been successfully developed. It was applied to the quantitative determination of specific IgG antibodies against Helicobacter pylori present in human serum samples. This system is a relevant alternative tool for the diagnostic of peptic ulcer and gastritis diseases produced by H. pylori, which are recognized risk factors for the development of gastric mucosa associated lymphoid tissue lymphoma and gastric adenocarcinoma. More than 50% of the population has been affected by H. pylori, one of the most widespread infections around the world. Our proposed system combines the LIF detection, which offers great sensibility with the specificity of the immunological reactions and the microfluidic technology. The device has a central channel (CC) with packed H. pylori antigen immobilized on 3-aminopropyl-modified controlled pore glass (AP-CPG). Antibodies in serum samples reacted immunologically with the immobilized antigen and then, they were determined using alkaline phosphatase (AP) enzyme-labeled second antibodies specific to human IgG. The 4-methylumbelliferyl phosphate (4-MUP), employed as enzymatic substrate, was converted to soluble fluorescent methylumbelliferone by AP, and this fluorescent product was finally quantified by LIF detection system. The calculated detection limits for LIF detection and the ELISA procedure were 0.17 and 2.1 U mL -1, respectively, and the within- and between-assay coefficients of variation were below 5.1%.
Fil: Seia, Marco Abel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Pereira, Sirley Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Carlos A. Fontán. Universidad Nacional de San Luis; Argentina
Fil: de Vito, Irma Esther. Universidad Nacional de San Luis; Argentina
Fil: Messina, Germán Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Materia
ENZYME IMMUNOASSAYS
GASTRIC DISEASES
HELICOBACTER PYLORI
IMMUNOSENSOR
LIF DETECTION
MICROFLUIDIC SYSTEM
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/92851
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/92851
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Laser-induced fluorescence integrated in a microfluidic immunosensor for quantification of human serum IgG antibodies to Helicobacter pyloriSeia, Marco AbelPereira, Sirley VanesaFontán, Carlos A.de Vito, Irma EstherMessina, Germán AlejandroRaba, JulioENZYME IMMUNOASSAYSGASTRIC DISEASESHELICOBACTER PYLORIIMMUNOSENSORLIF DETECTIONMICROFLUIDIC SYSTEMhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1A portable immunosensor coupled to laser-induced fluorescence (LIF) detection system has been successfully developed. It was applied to the quantitative determination of specific IgG antibodies against Helicobacter pylori present in human serum samples. This system is a relevant alternative tool for the diagnostic of peptic ulcer and gastritis diseases produced by H. pylori, which are recognized risk factors for the development of gastric mucosa associated lymphoid tissue lymphoma and gastric adenocarcinoma. More than 50% of the population has been affected by H. pylori, one of the most widespread infections around the world. Our proposed system combines the LIF detection, which offers great sensibility with the specificity of the immunological reactions and the microfluidic technology. The device has a central channel (CC) with packed H. pylori antigen immobilized on 3-aminopropyl-modified controlled pore glass (AP-CPG). Antibodies in serum samples reacted immunologically with the immobilized antigen and then, they were determined using alkaline phosphatase (AP) enzyme-labeled second antibodies specific to human IgG. The 4-methylumbelliferyl phosphate (4-MUP), employed as enzymatic substrate, was converted to soluble fluorescent methylumbelliferone by AP, and this fluorescent product was finally quantified by LIF detection system. The calculated detection limits for LIF detection and the ELISA procedure were 0.17 and 2.1 U mL -1, respectively, and the within- and between-assay coefficients of variation were below 5.1%.Fil: Seia, Marco Abel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Pereira, Sirley Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Carlos A. Fontán. Universidad Nacional de San Luis; ArgentinaFil: de Vito, Irma Esther. Universidad Nacional de San Luis; ArgentinaFil: Messina, Germán Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaElsevier Science Sa2012-06-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/92851Seia, Marco Abel; Pereira, Sirley Vanesa; Fontán, Carlos A.; de Vito, Irma Esther; Messina, Germán Alejandro; et al.; Laser-induced fluorescence integrated in a microfluidic immunosensor for quantification of human serum IgG antibodies to Helicobacter pylori; Elsevier Science Sa; Sensors and Actuators B: Chemical; 168; 15-6-2012; 297-3020925-4005CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.snb.2012.04.026info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0925400512003759info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:10:02Zoai:ri.conicet.gov.ar:11336/92851instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:10:02.966CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Laser-induced fluorescence integrated in a microfluidic immunosensor for quantification of human serum IgG antibodies to Helicobacter pylori
title Laser-induced fluorescence integrated in a microfluidic immunosensor for quantification of human serum IgG antibodies to Helicobacter pylori
spellingShingle Laser-induced fluorescence integrated in a microfluidic immunosensor for quantification of human serum IgG antibodies to Helicobacter pylori
Seia, Marco Abel
ENZYME IMMUNOASSAYS
GASTRIC DISEASES
HELICOBACTER PYLORI
IMMUNOSENSOR
LIF DETECTION
MICROFLUIDIC SYSTEM
title_short Laser-induced fluorescence integrated in a microfluidic immunosensor for quantification of human serum IgG antibodies to Helicobacter pylori
title_full Laser-induced fluorescence integrated in a microfluidic immunosensor for quantification of human serum IgG antibodies to Helicobacter pylori
title_fullStr Laser-induced fluorescence integrated in a microfluidic immunosensor for quantification of human serum IgG antibodies to Helicobacter pylori
title_full_unstemmed Laser-induced fluorescence integrated in a microfluidic immunosensor for quantification of human serum IgG antibodies to Helicobacter pylori
title_sort Laser-induced fluorescence integrated in a microfluidic immunosensor for quantification of human serum IgG antibodies to Helicobacter pylori
dc.creator.none.fl_str_mv Seia, Marco Abel
Pereira, Sirley Vanesa
Fontán, Carlos A.
de Vito, Irma Esther
Messina, Germán Alejandro
Raba, Julio
author Seia, Marco Abel
author_facet Seia, Marco Abel
Pereira, Sirley Vanesa
Fontán, Carlos A.
de Vito, Irma Esther
Messina, Germán Alejandro
Raba, Julio
author_role author
author2 Pereira, Sirley Vanesa
Fontán, Carlos A.
de Vito, Irma Esther
Messina, Germán Alejandro
Raba, Julio
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv ENZYME IMMUNOASSAYS
GASTRIC DISEASES
HELICOBACTER PYLORI
IMMUNOSENSOR
LIF DETECTION
MICROFLUIDIC SYSTEM
topic ENZYME IMMUNOASSAYS
GASTRIC DISEASES
HELICOBACTER PYLORI
IMMUNOSENSOR
LIF DETECTION
MICROFLUIDIC SYSTEM
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A portable immunosensor coupled to laser-induced fluorescence (LIF) detection system has been successfully developed. It was applied to the quantitative determination of specific IgG antibodies against Helicobacter pylori present in human serum samples. This system is a relevant alternative tool for the diagnostic of peptic ulcer and gastritis diseases produced by H. pylori, which are recognized risk factors for the development of gastric mucosa associated lymphoid tissue lymphoma and gastric adenocarcinoma. More than 50% of the population has been affected by H. pylori, one of the most widespread infections around the world. Our proposed system combines the LIF detection, which offers great sensibility with the specificity of the immunological reactions and the microfluidic technology. The device has a central channel (CC) with packed H. pylori antigen immobilized on 3-aminopropyl-modified controlled pore glass (AP-CPG). Antibodies in serum samples reacted immunologically with the immobilized antigen and then, they were determined using alkaline phosphatase (AP) enzyme-labeled second antibodies specific to human IgG. The 4-methylumbelliferyl phosphate (4-MUP), employed as enzymatic substrate, was converted to soluble fluorescent methylumbelliferone by AP, and this fluorescent product was finally quantified by LIF detection system. The calculated detection limits for LIF detection and the ELISA procedure were 0.17 and 2.1 U mL -1, respectively, and the within- and between-assay coefficients of variation were below 5.1%.
Fil: Seia, Marco Abel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Pereira, Sirley Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Carlos A. Fontán. Universidad Nacional de San Luis; Argentina
Fil: de Vito, Irma Esther. Universidad Nacional de San Luis; Argentina
Fil: Messina, Germán Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
description A portable immunosensor coupled to laser-induced fluorescence (LIF) detection system has been successfully developed. It was applied to the quantitative determination of specific IgG antibodies against Helicobacter pylori present in human serum samples. This system is a relevant alternative tool for the diagnostic of peptic ulcer and gastritis diseases produced by H. pylori, which are recognized risk factors for the development of gastric mucosa associated lymphoid tissue lymphoma and gastric adenocarcinoma. More than 50% of the population has been affected by H. pylori, one of the most widespread infections around the world. Our proposed system combines the LIF detection, which offers great sensibility with the specificity of the immunological reactions and the microfluidic technology. The device has a central channel (CC) with packed H. pylori antigen immobilized on 3-aminopropyl-modified controlled pore glass (AP-CPG). Antibodies in serum samples reacted immunologically with the immobilized antigen and then, they were determined using alkaline phosphatase (AP) enzyme-labeled second antibodies specific to human IgG. The 4-methylumbelliferyl phosphate (4-MUP), employed as enzymatic substrate, was converted to soluble fluorescent methylumbelliferone by AP, and this fluorescent product was finally quantified by LIF detection system. The calculated detection limits for LIF detection and the ELISA procedure were 0.17 and 2.1 U mL -1, respectively, and the within- and between-assay coefficients of variation were below 5.1%.
publishDate 2012
dc.date.none.fl_str_mv 2012-06-15
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/92851
Seia, Marco Abel; Pereira, Sirley Vanesa; Fontán, Carlos A.; de Vito, Irma Esther; Messina, Germán Alejandro; et al.; Laser-induced fluorescence integrated in a microfluidic immunosensor for quantification of human serum IgG antibodies to Helicobacter pylori; Elsevier Science Sa; Sensors and Actuators B: Chemical; 168; 15-6-2012; 297-302
0925-4005
CONICET Digital
CONICET
url http://hdl.handle.net/11336/92851
identifier_str_mv Seia, Marco Abel; Pereira, Sirley Vanesa; Fontán, Carlos A.; de Vito, Irma Esther; Messina, Germán Alejandro; et al.; Laser-induced fluorescence integrated in a microfluidic immunosensor for quantification of human serum IgG antibodies to Helicobacter pylori; Elsevier Science Sa; Sensors and Actuators B: Chemical; 168; 15-6-2012; 297-302
0925-4005
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.snb.2012.04.026
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0925400512003759
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Sa
publisher.none.fl_str_mv Elsevier Science Sa
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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