Soil-mimicking microfluidic devices reveal restricted flagellar motility of Bradyrhizobium diazoefficiens under microconfinement

Autores
Pires Monteiro, Moniellen; Carrillo Mora, Juan Pablo; Gutiérrez, Nahuel; Montagna, Sofía; Lodeiro, Anibal; Cordero, María Luisa; Marconi, Veronica Iris
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bradyrhizobium diazoefficiens is a nitrogen-fixing symbiont of soybean, worldwide used as biofertilizer. This soil bacterium possesses two flagellar systems enabling it to swim in water-saturated soils. However, the motility in soil pores, which may be crucial for competitiveness for root nodulation, is difficult to predict. To address this gap, we fabricated microfluidic devices with networks of connected microchannels surrounding grains. In them, we directly visualise bacterial behaviour in transparent geometries mimicking minimalist soils-on-a-chip (SOCs). We measured the population velocities and changes of direction for two strains: the wild-type and a mutant with only a subpolar flagellum. A detailed statistical analysis revealed that both strains exhibited reduced speeds and increased changes of direction of 180°, in channels of decreasing cross sectional area, down to a few microns in width. Interestingly, while the wild-type strain displayed faster swimming in unconfined spaces, this advantage was negated in the SOCs with the narrowest microchannels. We employed the measured motility parameters to propose a realistic model and simulate B. diazoefficiens confined dynamics being able to reproduce their behaviour, which additionally can be extended enabling further predictions for long time and macro scales. This multidisciplinary work, combining design, microfabrication, microbiology and modelling, offers useful methods to study soil bacteria and may be readily extended to other beneficial/harmful soil species.
Fil: Pires Monteiro, Moniellen. Universidad de Chile.; Chile
Fil: Carrillo Mora, Juan Pablo. Universidad de Chile.; Chile
Fil: Gutiérrez, Nahuel. Universidad Nacional de Córdoba. Facultad de Matemática, Astronomía y Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Física Enrique Gaviola. Universidad Nacional de Córdoba. Instituto de Física Enrique Gaviola; Argentina
Fil: Montagna, Sofía. Universidad Nacional de Córdoba. Facultad de Matemática, Astronomía y Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Lodeiro, Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Cordero, María Luisa. Universidad de Chile.; Chile
Fil: Marconi, Veronica Iris. Universidad Nacional de Córdoba. Facultad de Matemática, Astronomía y Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Física Enrique Gaviola. Universidad Nacional de Córdoba. Instituto de Física Enrique Gaviola; Argentina
Materia
SOIL-BACTERIA
SOIL-ON-A-CHIP
MICROFLUIDICS
BIOFERTILIZER
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/265258
Acceder
id CONICETDig_9c0d1f7394552538e105cd38f9f26726
oai_identifier_str oai:ri.conicet.gov.ar:11336/265258
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Soil-mimicking microfluidic devices reveal restricted flagellar motility of Bradyrhizobium diazoefficiens under microconfinementPires Monteiro, MoniellenCarrillo Mora, Juan PabloGutiérrez, NahuelMontagna, SofíaLodeiro, AnibalCordero, María LuisaMarconi, Veronica IrisSOIL-BACTERIASOIL-ON-A-CHIPMICROFLUIDICSBIOFERTILIZERhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Bradyrhizobium diazoefficiens is a nitrogen-fixing symbiont of soybean, worldwide used as biofertilizer. This soil bacterium possesses two flagellar systems enabling it to swim in water-saturated soils. However, the motility in soil pores, which may be crucial for competitiveness for root nodulation, is difficult to predict. To address this gap, we fabricated microfluidic devices with networks of connected microchannels surrounding grains. In them, we directly visualise bacterial behaviour in transparent geometries mimicking minimalist soils-on-a-chip (SOCs). We measured the population velocities and changes of direction for two strains: the wild-type and a mutant with only a subpolar flagellum. A detailed statistical analysis revealed that both strains exhibited reduced speeds and increased changes of direction of 180°, in channels of decreasing cross sectional area, down to a few microns in width. Interestingly, while the wild-type strain displayed faster swimming in unconfined spaces, this advantage was negated in the SOCs with the narrowest microchannels. We employed the measured motility parameters to propose a realistic model and simulate B. diazoefficiens confined dynamics being able to reproduce their behaviour, which additionally can be extended enabling further predictions for long time and macro scales. This multidisciplinary work, combining design, microfabrication, microbiology and modelling, offers useful methods to study soil bacteria and may be readily extended to other beneficial/harmful soil species.Fil: Pires Monteiro, Moniellen. Universidad de Chile.; ChileFil: Carrillo Mora, Juan Pablo. Universidad de Chile.; ChileFil: Gutiérrez, Nahuel. Universidad Nacional de Córdoba. Facultad de Matemática, Astronomía y Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Física Enrique Gaviola. Universidad Nacional de Córdoba. Instituto de Física Enrique Gaviola; ArgentinaFil: Montagna, Sofía. Universidad Nacional de Córdoba. Facultad de Matemática, Astronomía y Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Lodeiro, Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Cordero, María Luisa. Universidad de Chile.; ChileFil: Marconi, Veronica Iris. Universidad Nacional de Córdoba. Facultad de Matemática, Astronomía y Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Física Enrique Gaviola. Universidad Nacional de Córdoba. Instituto de Física Enrique Gaviola; ArgentinaSpringer2024-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/265258Pires Monteiro, Moniellen; Carrillo Mora, Juan Pablo; Gutiérrez, Nahuel; Montagna, Sofía; Lodeiro, Anibal; et al.; Soil-mimicking microfluidic devices reveal restricted flagellar motility of Bradyrhizobium diazoefficiens under microconfinement; Springer; Communications Biology; 8; 662; 1-2024; 1-132692-8205CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.biorxiv.org/content/10.1101/2023.12.29.573673v2info:eu-repo/semantics/altIdentifier/doi/10.1101/2023.12.29.573673info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:27:45Zoai:ri.conicet.gov.ar:11336/265258instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:27:45.78CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Soil-mimicking microfluidic devices reveal restricted flagellar motility of Bradyrhizobium diazoefficiens under microconfinement
title Soil-mimicking microfluidic devices reveal restricted flagellar motility of Bradyrhizobium diazoefficiens under microconfinement
spellingShingle Soil-mimicking microfluidic devices reveal restricted flagellar motility of Bradyrhizobium diazoefficiens under microconfinement
Pires Monteiro, Moniellen
SOIL-BACTERIA
SOIL-ON-A-CHIP
MICROFLUIDICS
BIOFERTILIZER
title_short Soil-mimicking microfluidic devices reveal restricted flagellar motility of Bradyrhizobium diazoefficiens under microconfinement
title_full Soil-mimicking microfluidic devices reveal restricted flagellar motility of Bradyrhizobium diazoefficiens under microconfinement
title_fullStr Soil-mimicking microfluidic devices reveal restricted flagellar motility of Bradyrhizobium diazoefficiens under microconfinement
title_full_unstemmed Soil-mimicking microfluidic devices reveal restricted flagellar motility of Bradyrhizobium diazoefficiens under microconfinement
title_sort Soil-mimicking microfluidic devices reveal restricted flagellar motility of Bradyrhizobium diazoefficiens under microconfinement
dc.creator.none.fl_str_mv Pires Monteiro, Moniellen
Carrillo Mora, Juan Pablo
Gutiérrez, Nahuel
Montagna, Sofía
Lodeiro, Anibal
Cordero, María Luisa
Marconi, Veronica Iris
author Pires Monteiro, Moniellen
author_facet Pires Monteiro, Moniellen
Carrillo Mora, Juan Pablo
Gutiérrez, Nahuel
Montagna, Sofía
Lodeiro, Anibal
Cordero, María Luisa
Marconi, Veronica Iris
author_role author
author2 Carrillo Mora, Juan Pablo
Gutiérrez, Nahuel
Montagna, Sofía
Lodeiro, Anibal
Cordero, María Luisa
Marconi, Veronica Iris
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv SOIL-BACTERIA
SOIL-ON-A-CHIP
MICROFLUIDICS
BIOFERTILIZER
topic SOIL-BACTERIA
SOIL-ON-A-CHIP
MICROFLUIDICS
BIOFERTILIZER
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Bradyrhizobium diazoefficiens is a nitrogen-fixing symbiont of soybean, worldwide used as biofertilizer. This soil bacterium possesses two flagellar systems enabling it to swim in water-saturated soils. However, the motility in soil pores, which may be crucial for competitiveness for root nodulation, is difficult to predict. To address this gap, we fabricated microfluidic devices with networks of connected microchannels surrounding grains. In them, we directly visualise bacterial behaviour in transparent geometries mimicking minimalist soils-on-a-chip (SOCs). We measured the population velocities and changes of direction for two strains: the wild-type and a mutant with only a subpolar flagellum. A detailed statistical analysis revealed that both strains exhibited reduced speeds and increased changes of direction of 180°, in channels of decreasing cross sectional area, down to a few microns in width. Interestingly, while the wild-type strain displayed faster swimming in unconfined spaces, this advantage was negated in the SOCs with the narrowest microchannels. We employed the measured motility parameters to propose a realistic model and simulate B. diazoefficiens confined dynamics being able to reproduce their behaviour, which additionally can be extended enabling further predictions for long time and macro scales. This multidisciplinary work, combining design, microfabrication, microbiology and modelling, offers useful methods to study soil bacteria and may be readily extended to other beneficial/harmful soil species.
Fil: Pires Monteiro, Moniellen. Universidad de Chile.; Chile
Fil: Carrillo Mora, Juan Pablo. Universidad de Chile.; Chile
Fil: Gutiérrez, Nahuel. Universidad Nacional de Córdoba. Facultad de Matemática, Astronomía y Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Física Enrique Gaviola. Universidad Nacional de Córdoba. Instituto de Física Enrique Gaviola; Argentina
Fil: Montagna, Sofía. Universidad Nacional de Córdoba. Facultad de Matemática, Astronomía y Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Lodeiro, Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Cordero, María Luisa. Universidad de Chile.; Chile
Fil: Marconi, Veronica Iris. Universidad Nacional de Córdoba. Facultad de Matemática, Astronomía y Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Física Enrique Gaviola. Universidad Nacional de Córdoba. Instituto de Física Enrique Gaviola; Argentina
description Bradyrhizobium diazoefficiens is a nitrogen-fixing symbiont of soybean, worldwide used as biofertilizer. This soil bacterium possesses two flagellar systems enabling it to swim in water-saturated soils. However, the motility in soil pores, which may be crucial for competitiveness for root nodulation, is difficult to predict. To address this gap, we fabricated microfluidic devices with networks of connected microchannels surrounding grains. In them, we directly visualise bacterial behaviour in transparent geometries mimicking minimalist soils-on-a-chip (SOCs). We measured the population velocities and changes of direction for two strains: the wild-type and a mutant with only a subpolar flagellum. A detailed statistical analysis revealed that both strains exhibited reduced speeds and increased changes of direction of 180°, in channels of decreasing cross sectional area, down to a few microns in width. Interestingly, while the wild-type strain displayed faster swimming in unconfined spaces, this advantage was negated in the SOCs with the narrowest microchannels. We employed the measured motility parameters to propose a realistic model and simulate B. diazoefficiens confined dynamics being able to reproduce their behaviour, which additionally can be extended enabling further predictions for long time and macro scales. This multidisciplinary work, combining design, microfabrication, microbiology and modelling, offers useful methods to study soil bacteria and may be readily extended to other beneficial/harmful soil species.
publishDate 2024
dc.date.none.fl_str_mv 2024-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/265258
Pires Monteiro, Moniellen; Carrillo Mora, Juan Pablo; Gutiérrez, Nahuel; Montagna, Sofía; Lodeiro, Anibal; et al.; Soil-mimicking microfluidic devices reveal restricted flagellar motility of Bradyrhizobium diazoefficiens under microconfinement; Springer; Communications Biology; 8; 662; 1-2024; 1-13
2692-8205
CONICET Digital
CONICET
url http://hdl.handle.net/11336/265258
identifier_str_mv Pires Monteiro, Moniellen; Carrillo Mora, Juan Pablo; Gutiérrez, Nahuel; Montagna, Sofía; Lodeiro, Anibal; et al.; Soil-mimicking microfluidic devices reveal restricted flagellar motility of Bradyrhizobium diazoefficiens under microconfinement; Springer; Communications Biology; 8; 662; 1-2024; 1-13
2692-8205
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.biorxiv.org/content/10.1101/2023.12.29.573673v2
info:eu-repo/semantics/altIdentifier/doi/10.1101/2023.12.29.573673
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846781849118965760
score 12.982451

Publicaciones similares