Evaluation of cell culture in microfluidic chips for application in monoclonal antibody production

Autores
Peñaherrera Pazmiño, Ana Belén; Payés, Cristian; Sierra Rodero, Marina; Vega, M.; Rosero, G.; Lerner, Betiana; Helguera, Gustavo Fernando; Pérez, M. S.
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Microfluidic chips are useful devices for cell culture that allow cell growth under highly controlled conditions, as is required for production of therapeutic recombinant proteins. To understand the optimal conditions for growth of cells amenable of recombinant protein expression in these devices,we culturedHEK-293T cells under different microfluidic experimental conditions. The cells were cultured in polymethyl methacrylate (PMMA) and polydi-methylsiloxane (PDMS)microdevices, in the absence or presence of the cell adhesion agent poly-D-lysine. Different microchannel geometries and thicknesses, as well as the influence of the flow rate have also been tested, showing their great influence in cell adhesion and growth. Results show that the presence of poly-D-lysine improves the adhesion and viability of the cells in continuous or discontinuous flow. Moreover, the optimal adhesion of cells was observed in the corners of themicrochannels, as well as in wide channels possibly due to the decrease in the flow rate in these areas. These studies provide insight into the optimal architecture of microchannels for long-term culture of adherent cells in order to use microfluidics devices as bioreactors for monoclonal antibodies production.
Fil: Peñaherrera Pazmiño, Ana Belén. Universidad Tecnológica Nacional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Payés, Cristian. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Sierra Rodero, Marina. Universidad Tecnológica Nacional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Vega, M.. Universidad Tecnológica Nacional; Argentina
Fil: Rosero, G.. Universidad Tecnológica Nacional; Argentina. Universidad de Buenos Aires; Argentina
Fil: Lerner, Betiana. Universidad Tecnológica Nacional; Argentina. Universidad de Buenos Aires; Argentina
Fil: Helguera, Gustavo Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Pérez, M. S.. Universidad Tecnológica Nacional; Argentina. Universidad de Buenos Aires; Argentina
Materia
MICROFLUIDICS
MICROCHANNEL
LAB ON A CHIP
CELL CULTURE
HEK-293T CELLS
Nivel de accesibilidad
acceso embargado
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/23347
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/23347
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Evaluation of cell culture in microfluidic chips for application in monoclonal antibody productionPeñaherrera Pazmiño, Ana BelénPayés, CristianSierra Rodero, MarinaVega, M.Rosero, G.Lerner, BetianaHelguera, Gustavo FernandoPérez, M. S.MICROFLUIDICSMICROCHANNELLAB ON A CHIPCELL CULTUREHEK-293T CELLShttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2Microfluidic chips are useful devices for cell culture that allow cell growth under highly controlled conditions, as is required for production of therapeutic recombinant proteins. To understand the optimal conditions for growth of cells amenable of recombinant protein expression in these devices,we culturedHEK-293T cells under different microfluidic experimental conditions. The cells were cultured in polymethyl methacrylate (PMMA) and polydi-methylsiloxane (PDMS)microdevices, in the absence or presence of the cell adhesion agent poly-D-lysine. Different microchannel geometries and thicknesses, as well as the influence of the flow rate have also been tested, showing their great influence in cell adhesion and growth. Results show that the presence of poly-D-lysine improves the adhesion and viability of the cells in continuous or discontinuous flow. Moreover, the optimal adhesion of cells was observed in the corners of themicrochannels, as well as in wide channels possibly due to the decrease in the flow rate in these areas. These studies provide insight into the optimal architecture of microchannels for long-term culture of adherent cells in order to use microfluidics devices as bioreactors for monoclonal antibodies production.Fil: Peñaherrera Pazmiño, Ana Belén. Universidad Tecnológica Nacional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Payés, Cristian. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Sierra Rodero, Marina. Universidad Tecnológica Nacional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Vega, M.. Universidad Tecnológica Nacional; ArgentinaFil: Rosero, G.. Universidad Tecnológica Nacional; Argentina. Universidad de Buenos Aires; ArgentinaFil: Lerner, Betiana. Universidad Tecnológica Nacional; Argentina. Universidad de Buenos Aires; ArgentinaFil: Helguera, Gustavo Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Pérez, M. S.. Universidad Tecnológica Nacional; Argentina. Universidad de Buenos Aires; ArgentinaElsevier Science2016-03-31info:eu-repo/date/embargoEnd/2018-04-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/23347Peñaherrera Pazmiño, Ana Belén; Payés, Cristian; Sierra Rodero, Marina; Vega, M.; Rosero, G.; et al.; Evaluation of cell culture in microfluidic chips for application in monoclonal antibody production; Elsevier Science; Microelectronic Engineering; 158; 31-3-2016; 126-1290167-9317CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0167931716301861info:eu-repo/semantics/altIdentifier/doi/10.1016/j.mee.2016.03.059info:eu-repo/semantics/embargoedAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:57:53Zoai:ri.conicet.gov.ar:11336/23347instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:57:53.632CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Evaluation of cell culture in microfluidic chips for application in monoclonal antibody production
title Evaluation of cell culture in microfluidic chips for application in monoclonal antibody production
spellingShingle Evaluation of cell culture in microfluidic chips for application in monoclonal antibody production
Peñaherrera Pazmiño, Ana Belén
MICROFLUIDICS
MICROCHANNEL
LAB ON A CHIP
CELL CULTURE
HEK-293T CELLS
title_short Evaluation of cell culture in microfluidic chips for application in monoclonal antibody production
title_full Evaluation of cell culture in microfluidic chips for application in monoclonal antibody production
title_fullStr Evaluation of cell culture in microfluidic chips for application in monoclonal antibody production
title_full_unstemmed Evaluation of cell culture in microfluidic chips for application in monoclonal antibody production
title_sort Evaluation of cell culture in microfluidic chips for application in monoclonal antibody production
dc.creator.none.fl_str_mv Peñaherrera Pazmiño, Ana Belén
Payés, Cristian
Sierra Rodero, Marina
Vega, M.
Rosero, G.
Lerner, Betiana
Helguera, Gustavo Fernando
Pérez, M. S.
author Peñaherrera Pazmiño, Ana Belén
author_facet Peñaherrera Pazmiño, Ana Belén
Payés, Cristian
Sierra Rodero, Marina
Vega, M.
Rosero, G.
Lerner, Betiana
Helguera, Gustavo Fernando
Pérez, M. S.
author_role author
author2 Payés, Cristian
Sierra Rodero, Marina
Vega, M.
Rosero, G.
Lerner, Betiana
Helguera, Gustavo Fernando
Pérez, M. S.
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv MICROFLUIDICS
MICROCHANNEL
LAB ON A CHIP
CELL CULTURE
HEK-293T CELLS
topic MICROFLUIDICS
MICROCHANNEL
LAB ON A CHIP
CELL CULTURE
HEK-293T CELLS
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Microfluidic chips are useful devices for cell culture that allow cell growth under highly controlled conditions, as is required for production of therapeutic recombinant proteins. To understand the optimal conditions for growth of cells amenable of recombinant protein expression in these devices,we culturedHEK-293T cells under different microfluidic experimental conditions. The cells were cultured in polymethyl methacrylate (PMMA) and polydi-methylsiloxane (PDMS)microdevices, in the absence or presence of the cell adhesion agent poly-D-lysine. Different microchannel geometries and thicknesses, as well as the influence of the flow rate have also been tested, showing their great influence in cell adhesion and growth. Results show that the presence of poly-D-lysine improves the adhesion and viability of the cells in continuous or discontinuous flow. Moreover, the optimal adhesion of cells was observed in the corners of themicrochannels, as well as in wide channels possibly due to the decrease in the flow rate in these areas. These studies provide insight into the optimal architecture of microchannels for long-term culture of adherent cells in order to use microfluidics devices as bioreactors for monoclonal antibodies production.
Fil: Peñaherrera Pazmiño, Ana Belén. Universidad Tecnológica Nacional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Payés, Cristian. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Sierra Rodero, Marina. Universidad Tecnológica Nacional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Vega, M.. Universidad Tecnológica Nacional; Argentina
Fil: Rosero, G.. Universidad Tecnológica Nacional; Argentina. Universidad de Buenos Aires; Argentina
Fil: Lerner, Betiana. Universidad Tecnológica Nacional; Argentina. Universidad de Buenos Aires; Argentina
Fil: Helguera, Gustavo Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Pérez, M. S.. Universidad Tecnológica Nacional; Argentina. Universidad de Buenos Aires; Argentina
description Microfluidic chips are useful devices for cell culture that allow cell growth under highly controlled conditions, as is required for production of therapeutic recombinant proteins. To understand the optimal conditions for growth of cells amenable of recombinant protein expression in these devices,we culturedHEK-293T cells under different microfluidic experimental conditions. The cells were cultured in polymethyl methacrylate (PMMA) and polydi-methylsiloxane (PDMS)microdevices, in the absence or presence of the cell adhesion agent poly-D-lysine. Different microchannel geometries and thicknesses, as well as the influence of the flow rate have also been tested, showing their great influence in cell adhesion and growth. Results show that the presence of poly-D-lysine improves the adhesion and viability of the cells in continuous or discontinuous flow. Moreover, the optimal adhesion of cells was observed in the corners of themicrochannels, as well as in wide channels possibly due to the decrease in the flow rate in these areas. These studies provide insight into the optimal architecture of microchannels for long-term culture of adherent cells in order to use microfluidics devices as bioreactors for monoclonal antibodies production.
publishDate 2016
dc.date.none.fl_str_mv 2016-03-31
info:eu-repo/date/embargoEnd/2018-04-30
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/23347
Peñaherrera Pazmiño, Ana Belén; Payés, Cristian; Sierra Rodero, Marina; Vega, M.; Rosero, G.; et al.; Evaluation of cell culture in microfluidic chips for application in monoclonal antibody production; Elsevier Science; Microelectronic Engineering; 158; 31-3-2016; 126-129
0167-9317
CONICET Digital
CONICET
url http://hdl.handle.net/11336/23347
identifier_str_mv Peñaherrera Pazmiño, Ana Belén; Payés, Cristian; Sierra Rodero, Marina; Vega, M.; Rosero, G.; et al.; Evaluation of cell culture in microfluidic chips for application in monoclonal antibody production; Elsevier Science; Microelectronic Engineering; 158; 31-3-2016; 126-129
0167-9317
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0167931716301861
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.mee.2016.03.059
dc.rights.none.fl_str_mv info:eu-repo/semantics/embargoedAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv embargoedAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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