Chlorpyrifos biodegradation and 3,5,6-trichloro-2-pyridinol production by actinobacteria isolated from soil

Autores
Briceño, G.; Fuentes, María Soledad; Palma, G.; Jorquera, M. A.; Amoroso, Maria Julia del R.; Diez, M. C.
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Chlorpyrifos (CP) is a widely used agricultural insecticide that is hazardous to both the environment and human health. Therefore, it is an essential to develop approaches to remove this compound from contaminated soils, water and sediments. In this study, actinobacteria were isolated from an agricultural soil that had received continuous applications of CP. Four strains were selected as a result of their tolerance to 50 mg L -1 of CP in agar plate and they were identified as Streptomyces sp. based on 16S rDNA. According to relationship of CP degradation and microbial growth studies, two isolates were selected and were named Streptomyces sp. strain AC5 and Streptomyces sp. strain AC7. The strains were cultivated in liquid medium with CP at concentrations of 25 mg L -1 and 50 mg L -1 for 72 h. The results indicated that both strains were able to rapidly degrade CP with about 90% degradation after 24 h of incubation. A different pattern of CP degradation was observed when its main metabolite, 3,5,6-trichloro-2-pyridinol (TCP) was monitored. A maximum concentration of 0.46 mg L -1 of TCP was produced by Streptomyces sp. strain AC5 and its concentration decreased as a function of time. In contrast, TCP production by Streptomyces sp. AC7 increased over time from 1.31 mg L -1 to 4.32 mg L -1. CP degradation was associated to microbial growth of the strains, pH modification, glucose consumption and organic acids excretion in the liquid medium. This work constitutes one of the few reports of Streptomyces as CP-degraders. Given the high CP degradation observed here, the Streptomycetes strains show a good potential as CP-degrading actinobacteria.
Fil: Briceño, G.. Universidad de la Frontera; Chile
Fil: Fuentes, María Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Planta Piloto de Procesos Industriales Microbiologicos; Argentina
Fil: Palma, G.. Universidad de la Frontera; Chile
Fil: Jorquera, M. A.. Universidad de la Frontera; Chile
Fil: Amoroso, Maria Julia del R.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Planta Piloto de Procesos Industriales Microbiologicos; Argentina
Fil: Diez, M. C.. Universidad de la Frontera; Chile
Materia
Bioremediation
Chlorpyrifos
Pesticides
Streptomyces Sp.
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/37694
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Chlorpyrifos biodegradation and 3,5,6-trichloro-2-pyridinol production by actinobacteria isolated from soilBriceño, G.Fuentes, María SoledadPalma, G.Jorquera, M. A.Amoroso, Maria Julia del R.Diez, M. C.BioremediationChlorpyrifosPesticidesStreptomyces Sp.https://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2Chlorpyrifos (CP) is a widely used agricultural insecticide that is hazardous to both the environment and human health. Therefore, it is an essential to develop approaches to remove this compound from contaminated soils, water and sediments. In this study, actinobacteria were isolated from an agricultural soil that had received continuous applications of CP. Four strains were selected as a result of their tolerance to 50 mg L -1 of CP in agar plate and they were identified as Streptomyces sp. based on 16S rDNA. According to relationship of CP degradation and microbial growth studies, two isolates were selected and were named Streptomyces sp. strain AC5 and Streptomyces sp. strain AC7. The strains were cultivated in liquid medium with CP at concentrations of 25 mg L -1 and 50 mg L -1 for 72 h. The results indicated that both strains were able to rapidly degrade CP with about 90% degradation after 24 h of incubation. A different pattern of CP degradation was observed when its main metabolite, 3,5,6-trichloro-2-pyridinol (TCP) was monitored. A maximum concentration of 0.46 mg L -1 of TCP was produced by Streptomyces sp. strain AC5 and its concentration decreased as a function of time. In contrast, TCP production by Streptomyces sp. AC7 increased over time from 1.31 mg L -1 to 4.32 mg L -1. CP degradation was associated to microbial growth of the strains, pH modification, glucose consumption and organic acids excretion in the liquid medium. This work constitutes one of the few reports of Streptomyces as CP-degraders. Given the high CP degradation observed here, the Streptomycetes strains show a good potential as CP-degrading actinobacteria.Fil: Briceño, G.. Universidad de la Frontera; ChileFil: Fuentes, María Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Planta Piloto de Procesos Industriales Microbiologicos; ArgentinaFil: Palma, G.. Universidad de la Frontera; ChileFil: Jorquera, M. A.. Universidad de la Frontera; ChileFil: Amoroso, Maria Julia del R.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Planta Piloto de Procesos Industriales Microbiologicos; ArgentinaFil: Diez, M. C.. Universidad de la Frontera; ChileElsevier2012-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/37694Briceño, G.; Fuentes, María Soledad; Palma, G.; Jorquera, M. A.; Amoroso, Maria Julia del R.; et al.; Chlorpyrifos biodegradation and 3,5,6-trichloro-2-pyridinol production by actinobacteria isolated from soil; Elsevier; International Biodeterioration and Biodegradation; 73; 9-2012; 1-70964-8305CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.ibiod.2012.06.002info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0964830512001357info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:46:16Zoai:ri.conicet.gov.ar:11336/37694instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:46:16.681CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Chlorpyrifos biodegradation and 3,5,6-trichloro-2-pyridinol production by actinobacteria isolated from soil
title Chlorpyrifos biodegradation and 3,5,6-trichloro-2-pyridinol production by actinobacteria isolated from soil
spellingShingle Chlorpyrifos biodegradation and 3,5,6-trichloro-2-pyridinol production by actinobacteria isolated from soil
Briceño, G.
Bioremediation
Chlorpyrifos
Pesticides
Streptomyces Sp.
title_short Chlorpyrifos biodegradation and 3,5,6-trichloro-2-pyridinol production by actinobacteria isolated from soil
title_full Chlorpyrifos biodegradation and 3,5,6-trichloro-2-pyridinol production by actinobacteria isolated from soil
title_fullStr Chlorpyrifos biodegradation and 3,5,6-trichloro-2-pyridinol production by actinobacteria isolated from soil
title_full_unstemmed Chlorpyrifos biodegradation and 3,5,6-trichloro-2-pyridinol production by actinobacteria isolated from soil
title_sort Chlorpyrifos biodegradation and 3,5,6-trichloro-2-pyridinol production by actinobacteria isolated from soil
dc.creator.none.fl_str_mv Briceño, G.
Fuentes, María Soledad
Palma, G.
Jorquera, M. A.
Amoroso, Maria Julia del R.
Diez, M. C.
author Briceño, G.
author_facet Briceño, G.
Fuentes, María Soledad
Palma, G.
Jorquera, M. A.
Amoroso, Maria Julia del R.
Diez, M. C.
author_role author
author2 Fuentes, María Soledad
Palma, G.
Jorquera, M. A.
Amoroso, Maria Julia del R.
Diez, M. C.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Bioremediation
Chlorpyrifos
Pesticides
Streptomyces Sp.
topic Bioremediation
Chlorpyrifos
Pesticides
Streptomyces Sp.
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Chlorpyrifos (CP) is a widely used agricultural insecticide that is hazardous to both the environment and human health. Therefore, it is an essential to develop approaches to remove this compound from contaminated soils, water and sediments. In this study, actinobacteria were isolated from an agricultural soil that had received continuous applications of CP. Four strains were selected as a result of their tolerance to 50 mg L -1 of CP in agar plate and they were identified as Streptomyces sp. based on 16S rDNA. According to relationship of CP degradation and microbial growth studies, two isolates were selected and were named Streptomyces sp. strain AC5 and Streptomyces sp. strain AC7. The strains were cultivated in liquid medium with CP at concentrations of 25 mg L -1 and 50 mg L -1 for 72 h. The results indicated that both strains were able to rapidly degrade CP with about 90% degradation after 24 h of incubation. A different pattern of CP degradation was observed when its main metabolite, 3,5,6-trichloro-2-pyridinol (TCP) was monitored. A maximum concentration of 0.46 mg L -1 of TCP was produced by Streptomyces sp. strain AC5 and its concentration decreased as a function of time. In contrast, TCP production by Streptomyces sp. AC7 increased over time from 1.31 mg L -1 to 4.32 mg L -1. CP degradation was associated to microbial growth of the strains, pH modification, glucose consumption and organic acids excretion in the liquid medium. This work constitutes one of the few reports of Streptomyces as CP-degraders. Given the high CP degradation observed here, the Streptomycetes strains show a good potential as CP-degrading actinobacteria.
Fil: Briceño, G.. Universidad de la Frontera; Chile
Fil: Fuentes, María Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Planta Piloto de Procesos Industriales Microbiologicos; Argentina
Fil: Palma, G.. Universidad de la Frontera; Chile
Fil: Jorquera, M. A.. Universidad de la Frontera; Chile
Fil: Amoroso, Maria Julia del R.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Planta Piloto de Procesos Industriales Microbiologicos; Argentina
Fil: Diez, M. C.. Universidad de la Frontera; Chile
description Chlorpyrifos (CP) is a widely used agricultural insecticide that is hazardous to both the environment and human health. Therefore, it is an essential to develop approaches to remove this compound from contaminated soils, water and sediments. In this study, actinobacteria were isolated from an agricultural soil that had received continuous applications of CP. Four strains were selected as a result of their tolerance to 50 mg L -1 of CP in agar plate and they were identified as Streptomyces sp. based on 16S rDNA. According to relationship of CP degradation and microbial growth studies, two isolates were selected and were named Streptomyces sp. strain AC5 and Streptomyces sp. strain AC7. The strains were cultivated in liquid medium with CP at concentrations of 25 mg L -1 and 50 mg L -1 for 72 h. The results indicated that both strains were able to rapidly degrade CP with about 90% degradation after 24 h of incubation. A different pattern of CP degradation was observed when its main metabolite, 3,5,6-trichloro-2-pyridinol (TCP) was monitored. A maximum concentration of 0.46 mg L -1 of TCP was produced by Streptomyces sp. strain AC5 and its concentration decreased as a function of time. In contrast, TCP production by Streptomyces sp. AC7 increased over time from 1.31 mg L -1 to 4.32 mg L -1. CP degradation was associated to microbial growth of the strains, pH modification, glucose consumption and organic acids excretion in the liquid medium. This work constitutes one of the few reports of Streptomyces as CP-degraders. Given the high CP degradation observed here, the Streptomycetes strains show a good potential as CP-degrading actinobacteria.
publishDate 2012
dc.date.none.fl_str_mv 2012-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/37694
Briceño, G.; Fuentes, María Soledad; Palma, G.; Jorquera, M. A.; Amoroso, Maria Julia del R.; et al.; Chlorpyrifos biodegradation and 3,5,6-trichloro-2-pyridinol production by actinobacteria isolated from soil; Elsevier; International Biodeterioration and Biodegradation; 73; 9-2012; 1-7
0964-8305
CONICET Digital
CONICET
url http://hdl.handle.net/11336/37694
identifier_str_mv Briceño, G.; Fuentes, María Soledad; Palma, G.; Jorquera, M. A.; Amoroso, Maria Julia del R.; et al.; Chlorpyrifos biodegradation and 3,5,6-trichloro-2-pyridinol production by actinobacteria isolated from soil; Elsevier; International Biodeterioration and Biodegradation; 73; 9-2012; 1-7
0964-8305
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ibiod.2012.06.002
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0964830512001357
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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