Quantification of neurotransmitters and metabolically related compounds at glassy carbon electrodes modified with bamboo-like carbon nanotubes dispersed in double stranded DNA

Autores
Gutierrez, Alejandro; Primo, Emiliano Nicolás; Eguílaz Rubio, Marcos; Parrado, Concepción; Rubianes, María Dolores; Rivas, Gustavo Adolfo
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This work reports the application of glassy carbon electrodes (GCE) modified with bamboo-like carbon nanotubes (bCNT) dispersed in calf-thymus double-stranded DNA (dsDNA) (GCE/bCNT-dsDNA) for the highly sensitive and selective quantification of neurotransmitters and metabolically related compounds by Adsorptive Stripping. Dopamine (Do) was quantified in the presence of its most important metabolite, 3,4-dihydroxyphenylacetic acid (dopac), while normetanephrine (NM) was determined in the presence of its precursor norepinephrine (NE). The accumulation of the analytes was performed at open circuit potential and the quantification was carried out, after medium exchange, in a 0.200 M acetate buffer solution pH 5.0 using Differential Pulse Voltammetry. The sensitivity for Do in the presence of 1.0 × 10− 5 M dopac was (7.1 ± 0.2) × 105 μAM− 1, (just 7.0% higher than the one obtained for Do alone), the detection limit was 68 nM, and the linear range 5.0 × 10− 7 M-7.0 × 10− 6 M. The sensitivities for NM in the absence and presence of NE were (2.8 ± 0.1) × 104 μAM− 1 and (2.6 ± 0.2) × 104 μAM− 1, with a linear range between 1.0 × 10− 6 and 2.0 × 10− 5 M, and a detection limit of 0.5 μM. The platform was successfully used for the quantification of Do and NM in enriched human urine samples.
Fil: Gutierrez, Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Primo, Emiliano Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Eguílaz Rubio, Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Parrado, Concepción. Universidad Complutense de Madrid; España
Fil: Rubianes, María Dolores. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Rivas, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Materia
Bamboo Carbon Nanotubes
Dopac
Dopamine
Dsdna
Electrochemical (Bio)Sensor
Norepinephrine
Normetanephrine
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/64189
Acceder
id CONICETDig_9b5687981583e2c6f962a89c86411982
oai_identifier_str oai:ri.conicet.gov.ar:11336/64189
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Quantification of neurotransmitters and metabolically related compounds at glassy carbon electrodes modified with bamboo-like carbon nanotubes dispersed in double stranded DNAGutierrez, AlejandroPrimo, Emiliano NicolásEguílaz Rubio, MarcosParrado, ConcepciónRubianes, María DoloresRivas, Gustavo AdolfoBamboo Carbon NanotubesDopacDopamineDsdnaElectrochemical (Bio)SensorNorepinephrineNormetanephrinehttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1https://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2This work reports the application of glassy carbon electrodes (GCE) modified with bamboo-like carbon nanotubes (bCNT) dispersed in calf-thymus double-stranded DNA (dsDNA) (GCE/bCNT-dsDNA) for the highly sensitive and selective quantification of neurotransmitters and metabolically related compounds by Adsorptive Stripping. Dopamine (Do) was quantified in the presence of its most important metabolite, 3,4-dihydroxyphenylacetic acid (dopac), while normetanephrine (NM) was determined in the presence of its precursor norepinephrine (NE). The accumulation of the analytes was performed at open circuit potential and the quantification was carried out, after medium exchange, in a 0.200 M acetate buffer solution pH 5.0 using Differential Pulse Voltammetry. The sensitivity for Do in the presence of 1.0 × 10− 5 M dopac was (7.1 ± 0.2) × 105 μAM− 1, (just 7.0% higher than the one obtained for Do alone), the detection limit was 68 nM, and the linear range 5.0 × 10− 7 M-7.0 × 10− 6 M. The sensitivities for NM in the absence and presence of NE were (2.8 ± 0.1) × 104 μAM− 1 and (2.6 ± 0.2) × 104 μAM− 1, with a linear range between 1.0 × 10− 6 and 2.0 × 10− 5 M, and a detection limit of 0.5 μM. The platform was successfully used for the quantification of Do and NM in enriched human urine samples.Fil: Gutierrez, Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Primo, Emiliano Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Eguílaz Rubio, Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Parrado, Concepción. Universidad Complutense de Madrid; EspañaFil: Rubianes, María Dolores. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Rivas, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaElsevier Science2017-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/64189Gutierrez, Alejandro; Primo, Emiliano Nicolás; Eguílaz Rubio, Marcos; Parrado, Concepción; Rubianes, María Dolores; et al.; Quantification of neurotransmitters and metabolically related compounds at glassy carbon electrodes modified with bamboo-like carbon nanotubes dispersed in double stranded DNA; Elsevier Science; Microchemical Journal; 130; 1-2017; 40-460026-265X1095-9149CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.microc.2016.08.001info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0026265X16302685info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:06:50Zoai:ri.conicet.gov.ar:11336/64189instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:06:50.852CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Quantification of neurotransmitters and metabolically related compounds at glassy carbon electrodes modified with bamboo-like carbon nanotubes dispersed in double stranded DNA
title Quantification of neurotransmitters and metabolically related compounds at glassy carbon electrodes modified with bamboo-like carbon nanotubes dispersed in double stranded DNA
spellingShingle Quantification of neurotransmitters and metabolically related compounds at glassy carbon electrodes modified with bamboo-like carbon nanotubes dispersed in double stranded DNA
Gutierrez, Alejandro
Bamboo Carbon Nanotubes
Dopac
Dopamine
Dsdna
Electrochemical (Bio)Sensor
Norepinephrine
Normetanephrine
title_short Quantification of neurotransmitters and metabolically related compounds at glassy carbon electrodes modified with bamboo-like carbon nanotubes dispersed in double stranded DNA
title_full Quantification of neurotransmitters and metabolically related compounds at glassy carbon electrodes modified with bamboo-like carbon nanotubes dispersed in double stranded DNA
title_fullStr Quantification of neurotransmitters and metabolically related compounds at glassy carbon electrodes modified with bamboo-like carbon nanotubes dispersed in double stranded DNA
title_full_unstemmed Quantification of neurotransmitters and metabolically related compounds at glassy carbon electrodes modified with bamboo-like carbon nanotubes dispersed in double stranded DNA
title_sort Quantification of neurotransmitters and metabolically related compounds at glassy carbon electrodes modified with bamboo-like carbon nanotubes dispersed in double stranded DNA
dc.creator.none.fl_str_mv Gutierrez, Alejandro
Primo, Emiliano Nicolás
Eguílaz Rubio, Marcos
Parrado, Concepción
Rubianes, María Dolores
Rivas, Gustavo Adolfo
author Gutierrez, Alejandro
author_facet Gutierrez, Alejandro
Primo, Emiliano Nicolás
Eguílaz Rubio, Marcos
Parrado, Concepción
Rubianes, María Dolores
Rivas, Gustavo Adolfo
author_role author
author2 Primo, Emiliano Nicolás
Eguílaz Rubio, Marcos
Parrado, Concepción
Rubianes, María Dolores
Rivas, Gustavo Adolfo
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Bamboo Carbon Nanotubes
Dopac
Dopamine
Dsdna
Electrochemical (Bio)Sensor
Norepinephrine
Normetanephrine
topic Bamboo Carbon Nanotubes
Dopac
Dopamine
Dsdna
Electrochemical (Bio)Sensor
Norepinephrine
Normetanephrine
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/2.10
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv This work reports the application of glassy carbon electrodes (GCE) modified with bamboo-like carbon nanotubes (bCNT) dispersed in calf-thymus double-stranded DNA (dsDNA) (GCE/bCNT-dsDNA) for the highly sensitive and selective quantification of neurotransmitters and metabolically related compounds by Adsorptive Stripping. Dopamine (Do) was quantified in the presence of its most important metabolite, 3,4-dihydroxyphenylacetic acid (dopac), while normetanephrine (NM) was determined in the presence of its precursor norepinephrine (NE). The accumulation of the analytes was performed at open circuit potential and the quantification was carried out, after medium exchange, in a 0.200 M acetate buffer solution pH 5.0 using Differential Pulse Voltammetry. The sensitivity for Do in the presence of 1.0 × 10− 5 M dopac was (7.1 ± 0.2) × 105 μAM− 1, (just 7.0% higher than the one obtained for Do alone), the detection limit was 68 nM, and the linear range 5.0 × 10− 7 M-7.0 × 10− 6 M. The sensitivities for NM in the absence and presence of NE were (2.8 ± 0.1) × 104 μAM− 1 and (2.6 ± 0.2) × 104 μAM− 1, with a linear range between 1.0 × 10− 6 and 2.0 × 10− 5 M, and a detection limit of 0.5 μM. The platform was successfully used for the quantification of Do and NM in enriched human urine samples.
Fil: Gutierrez, Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Primo, Emiliano Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Eguílaz Rubio, Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Parrado, Concepción. Universidad Complutense de Madrid; España
Fil: Rubianes, María Dolores. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Rivas, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
description This work reports the application of glassy carbon electrodes (GCE) modified with bamboo-like carbon nanotubes (bCNT) dispersed in calf-thymus double-stranded DNA (dsDNA) (GCE/bCNT-dsDNA) for the highly sensitive and selective quantification of neurotransmitters and metabolically related compounds by Adsorptive Stripping. Dopamine (Do) was quantified in the presence of its most important metabolite, 3,4-dihydroxyphenylacetic acid (dopac), while normetanephrine (NM) was determined in the presence of its precursor norepinephrine (NE). The accumulation of the analytes was performed at open circuit potential and the quantification was carried out, after medium exchange, in a 0.200 M acetate buffer solution pH 5.0 using Differential Pulse Voltammetry. The sensitivity for Do in the presence of 1.0 × 10− 5 M dopac was (7.1 ± 0.2) × 105 μAM− 1, (just 7.0% higher than the one obtained for Do alone), the detection limit was 68 nM, and the linear range 5.0 × 10− 7 M-7.0 × 10− 6 M. The sensitivities for NM in the absence and presence of NE were (2.8 ± 0.1) × 104 μAM− 1 and (2.6 ± 0.2) × 104 μAM− 1, with a linear range between 1.0 × 10− 6 and 2.0 × 10− 5 M, and a detection limit of 0.5 μM. The platform was successfully used for the quantification of Do and NM in enriched human urine samples.
publishDate 2017
dc.date.none.fl_str_mv 2017-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/64189
Gutierrez, Alejandro; Primo, Emiliano Nicolás; Eguílaz Rubio, Marcos; Parrado, Concepción; Rubianes, María Dolores; et al.; Quantification of neurotransmitters and metabolically related compounds at glassy carbon electrodes modified with bamboo-like carbon nanotubes dispersed in double stranded DNA; Elsevier Science; Microchemical Journal; 130; 1-2017; 40-46
0026-265X
1095-9149
CONICET Digital
CONICET
url http://hdl.handle.net/11336/64189
identifier_str_mv Gutierrez, Alejandro; Primo, Emiliano Nicolás; Eguílaz Rubio, Marcos; Parrado, Concepción; Rubianes, María Dolores; et al.; Quantification of neurotransmitters and metabolically related compounds at glassy carbon electrodes modified with bamboo-like carbon nanotubes dispersed in double stranded DNA; Elsevier Science; Microchemical Journal; 130; 1-2017; 40-46
0026-265X
1095-9149
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.microc.2016.08.001
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0026265X16302685
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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