Dopamine and glucose sensors based on glassy carbon electrodes modified with melanic polymers

Autores
González, Rosa; Sánchez, Alberto; Chicharro, Manuel; Rubianes, María Dolores; Rivas, Gustavo Adolfo
Año de publicación
2004
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This work deals with the study of polymers electrogenerated from different catechols at glassy carbon electrodes and the analytical applications of the resulting modified electrodes for dopamine quantification and glucose biosensing. The electropolymerization was performed from a 3.0 × 10 -3 M catechol solution (catechol, dopamine, norepinephrine, epinephrine or L-dopa in a 0.050 M phosphate buffer pH 7.40) by applying 1.00 V for 60 min. The properties of the polymers are very dependent on the nature of the catechol, L-dopa being the best. Glassy carbon electrodes modified with melanic polymers electrogenerated from L-dopa and norepinephrine were found to be suitable for dopamine determinations in flow systems, although the behavior was highly dependent on the nature of the monomer. Detection limits of 5.0 nM dopamine and interferences of 9.0 and 2.6% for 5.0 × 10-4 M ascorbic acid and 5.0 × 10-5 M dopac, respectively, were obtained at the glassy carbon electrode modified with a melanin-type polymer generated from L-dopa (using 1.0 × 10-3 M AA in the measurement solution). The advantages of using a melanin-type polymer generated from dopamine to improve the selectivity of glucose biosensors based on carbon paste electrodes containing Pt and glucose oxidase (GOx) are also discussed. The resulting bioelectrodes combines the high sensitivity of metallized electrodes with the selectivity given by the polymeric layer. They exhibit excellent performance for glucose with a rapid response (around 10 seconds per sample), a wide linear range (up to 2.5 × 10-2 M glucose), low detection limits (143 μM) and a highly reproducible response (R.S.D of 4.9%). The bioelectrodes are highly stable and almost free from the interference of large excess of easily oxidizable compounds found in biological fluids, such as ascorbic acid (AA), uric acid (UA) and acetaminophen.
Fil: González, Rosa. Universidad Autónoma de Madrid; España
Fil: Sánchez, Alberto. Universidad Autónoma de Madrid; España
Fil: Chicharro, Manuel. Universidad Autónoma de Madrid; España
Fil: Rubianes, María Dolores. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Rivas, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Materia
Ascorbic Acid
Dopac
Dopamine
Flow Injection
Glassy Carbon
Glucose Biosensor
Melanin
Metallized Electrode
Platinum
Polymer
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/36890
Acceder
id CONICETDig_ae51e92ba3973672f8aca6ad8fc66d4e
oai_identifier_str oai:ri.conicet.gov.ar:11336/36890
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Dopamine and glucose sensors based on glassy carbon electrodes modified with melanic polymersGonzález, RosaSánchez, AlbertoChicharro, ManuelRubianes, María DoloresRivas, Gustavo AdolfoAscorbic AcidDopacDopamineFlow InjectionGlassy CarbonGlucose BiosensorMelaninMetallized ElectrodePlatinumPolymerhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1This work deals with the study of polymers electrogenerated from different catechols at glassy carbon electrodes and the analytical applications of the resulting modified electrodes for dopamine quantification and glucose biosensing. The electropolymerization was performed from a 3.0 × 10 -3 M catechol solution (catechol, dopamine, norepinephrine, epinephrine or L-dopa in a 0.050 M phosphate buffer pH 7.40) by applying 1.00 V for 60 min. The properties of the polymers are very dependent on the nature of the catechol, L-dopa being the best. Glassy carbon electrodes modified with melanic polymers electrogenerated from L-dopa and norepinephrine were found to be suitable for dopamine determinations in flow systems, although the behavior was highly dependent on the nature of the monomer. Detection limits of 5.0 nM dopamine and interferences of 9.0 and 2.6% for 5.0 × 10-4 M ascorbic acid and 5.0 × 10-5 M dopac, respectively, were obtained at the glassy carbon electrode modified with a melanin-type polymer generated from L-dopa (using 1.0 × 10-3 M AA in the measurement solution). The advantages of using a melanin-type polymer generated from dopamine to improve the selectivity of glucose biosensors based on carbon paste electrodes containing Pt and glucose oxidase (GOx) are also discussed. The resulting bioelectrodes combines the high sensitivity of metallized electrodes with the selectivity given by the polymeric layer. They exhibit excellent performance for glucose with a rapid response (around 10 seconds per sample), a wide linear range (up to 2.5 × 10-2 M glucose), low detection limits (143 μM) and a highly reproducible response (R.S.D of 4.9%). The bioelectrodes are highly stable and almost free from the interference of large excess of easily oxidizable compounds found in biological fluids, such as ascorbic acid (AA), uric acid (UA) and acetaminophen.Fil: González, Rosa. Universidad Autónoma de Madrid; EspañaFil: Sánchez, Alberto. Universidad Autónoma de Madrid; EspañaFil: Chicharro, Manuel. Universidad Autónoma de Madrid; EspañaFil: Rubianes, María Dolores. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Rivas, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaWiley VCH Verlag2004-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/36890González, Rosa; Sánchez, Alberto; Chicharro, Manuel; Rubianes, María Dolores; Rivas, Gustavo Adolfo; Dopamine and glucose sensors based on glassy carbon electrodes modified with melanic polymers; Wiley VCH Verlag; Electroanalysis; 16; 15; 8-2004; 1244-12531040-0397CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/elan.200302939info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/elan.200302939/abstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:48:06Zoai:ri.conicet.gov.ar:11336/36890instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:48:07.268CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Dopamine and glucose sensors based on glassy carbon electrodes modified with melanic polymers
title Dopamine and glucose sensors based on glassy carbon electrodes modified with melanic polymers
spellingShingle Dopamine and glucose sensors based on glassy carbon electrodes modified with melanic polymers
González, Rosa
Ascorbic Acid
Dopac
Dopamine
Flow Injection
Glassy Carbon
Glucose Biosensor
Melanin
Metallized Electrode
Platinum
Polymer
title_short Dopamine and glucose sensors based on glassy carbon electrodes modified with melanic polymers
title_full Dopamine and glucose sensors based on glassy carbon electrodes modified with melanic polymers
title_fullStr Dopamine and glucose sensors based on glassy carbon electrodes modified with melanic polymers
title_full_unstemmed Dopamine and glucose sensors based on glassy carbon electrodes modified with melanic polymers
title_sort Dopamine and glucose sensors based on glassy carbon electrodes modified with melanic polymers
dc.creator.none.fl_str_mv González, Rosa
Sánchez, Alberto
Chicharro, Manuel
Rubianes, María Dolores
Rivas, Gustavo Adolfo
author González, Rosa
author_facet González, Rosa
Sánchez, Alberto
Chicharro, Manuel
Rubianes, María Dolores
Rivas, Gustavo Adolfo
author_role author
author2 Sánchez, Alberto
Chicharro, Manuel
Rubianes, María Dolores
Rivas, Gustavo Adolfo
author2_role author
author
author
author
dc.subject.none.fl_str_mv Ascorbic Acid
Dopac
Dopamine
Flow Injection
Glassy Carbon
Glucose Biosensor
Melanin
Metallized Electrode
Platinum
Polymer
topic Ascorbic Acid
Dopac
Dopamine
Flow Injection
Glassy Carbon
Glucose Biosensor
Melanin
Metallized Electrode
Platinum
Polymer
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv This work deals with the study of polymers electrogenerated from different catechols at glassy carbon electrodes and the analytical applications of the resulting modified electrodes for dopamine quantification and glucose biosensing. The electropolymerization was performed from a 3.0 × 10 -3 M catechol solution (catechol, dopamine, norepinephrine, epinephrine or L-dopa in a 0.050 M phosphate buffer pH 7.40) by applying 1.00 V for 60 min. The properties of the polymers are very dependent on the nature of the catechol, L-dopa being the best. Glassy carbon electrodes modified with melanic polymers electrogenerated from L-dopa and norepinephrine were found to be suitable for dopamine determinations in flow systems, although the behavior was highly dependent on the nature of the monomer. Detection limits of 5.0 nM dopamine and interferences of 9.0 and 2.6% for 5.0 × 10-4 M ascorbic acid and 5.0 × 10-5 M dopac, respectively, were obtained at the glassy carbon electrode modified with a melanin-type polymer generated from L-dopa (using 1.0 × 10-3 M AA in the measurement solution). The advantages of using a melanin-type polymer generated from dopamine to improve the selectivity of glucose biosensors based on carbon paste electrodes containing Pt and glucose oxidase (GOx) are also discussed. The resulting bioelectrodes combines the high sensitivity of metallized electrodes with the selectivity given by the polymeric layer. They exhibit excellent performance for glucose with a rapid response (around 10 seconds per sample), a wide linear range (up to 2.5 × 10-2 M glucose), low detection limits (143 μM) and a highly reproducible response (R.S.D of 4.9%). The bioelectrodes are highly stable and almost free from the interference of large excess of easily oxidizable compounds found in biological fluids, such as ascorbic acid (AA), uric acid (UA) and acetaminophen.
Fil: González, Rosa. Universidad Autónoma de Madrid; España
Fil: Sánchez, Alberto. Universidad Autónoma de Madrid; España
Fil: Chicharro, Manuel. Universidad Autónoma de Madrid; España
Fil: Rubianes, María Dolores. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Rivas, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
description This work deals with the study of polymers electrogenerated from different catechols at glassy carbon electrodes and the analytical applications of the resulting modified electrodes for dopamine quantification and glucose biosensing. The electropolymerization was performed from a 3.0 × 10 -3 M catechol solution (catechol, dopamine, norepinephrine, epinephrine or L-dopa in a 0.050 M phosphate buffer pH 7.40) by applying 1.00 V for 60 min. The properties of the polymers are very dependent on the nature of the catechol, L-dopa being the best. Glassy carbon electrodes modified with melanic polymers electrogenerated from L-dopa and norepinephrine were found to be suitable for dopamine determinations in flow systems, although the behavior was highly dependent on the nature of the monomer. Detection limits of 5.0 nM dopamine and interferences of 9.0 and 2.6% for 5.0 × 10-4 M ascorbic acid and 5.0 × 10-5 M dopac, respectively, were obtained at the glassy carbon electrode modified with a melanin-type polymer generated from L-dopa (using 1.0 × 10-3 M AA in the measurement solution). The advantages of using a melanin-type polymer generated from dopamine to improve the selectivity of glucose biosensors based on carbon paste electrodes containing Pt and glucose oxidase (GOx) are also discussed. The resulting bioelectrodes combines the high sensitivity of metallized electrodes with the selectivity given by the polymeric layer. They exhibit excellent performance for glucose with a rapid response (around 10 seconds per sample), a wide linear range (up to 2.5 × 10-2 M glucose), low detection limits (143 μM) and a highly reproducible response (R.S.D of 4.9%). The bioelectrodes are highly stable and almost free from the interference of large excess of easily oxidizable compounds found in biological fluids, such as ascorbic acid (AA), uric acid (UA) and acetaminophen.
publishDate 2004
dc.date.none.fl_str_mv 2004-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/36890
González, Rosa; Sánchez, Alberto; Chicharro, Manuel; Rubianes, María Dolores; Rivas, Gustavo Adolfo; Dopamine and glucose sensors based on glassy carbon electrodes modified with melanic polymers; Wiley VCH Verlag; Electroanalysis; 16; 15; 8-2004; 1244-1253
1040-0397
CONICET Digital
CONICET
url http://hdl.handle.net/11336/36890
identifier_str_mv González, Rosa; Sánchez, Alberto; Chicharro, Manuel; Rubianes, María Dolores; Rivas, Gustavo Adolfo; Dopamine and glucose sensors based on glassy carbon electrodes modified with melanic polymers; Wiley VCH Verlag; Electroanalysis; 16; 15; 8-2004; 1244-1253
1040-0397
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/elan.200302939
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/elan.200302939/abstract
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley VCH Verlag
publisher.none.fl_str_mv Wiley VCH Verlag
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083000060608512
score 13.22299

Publicaciones similares