Dictyota dichotoma (phaeophyceae) methanolic extracts exhibit antitumoral effects on breast cancer cells and induce osteoblasts differentiation

Autores
Lezcano, Virginia Alicia; Mariani, Florencia; Fernández, Carolina; Parodi, Elisa Rosalia; Morelli, Susana Ana
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
There is a growing interest in macroalgae as natural products with antioxidant and anticancer activity. In this work, we studied the anti-tumoral effect of an algal extract (AE) derived from the marine alga Dictyota dichotoma on human breast cancer cells (MCF-7). We also evaluated the cytotoxic effects on non-tumorigenic cells and the effects on ostoblastogenesis in vitro. We found that the AE contains high levels of polyphenols and anti-oxidant activity measured by DPPH and Folin-Ciocalteu methods, respectively. Using trypan blue and MTS assays we demonstrated a significant inhibition of MCF-7 cell proliferation and viability. The changes in protein phosphorylation levels were examined through Western blot analysis, finding a decrease of phosphorylated AKT (Ser473) and its target molecule BAD (Ser136). In addition, AE inhibits cell migration determined through the wound healing assay and decreases cellular adhesion at all concentrations probed. Interestingly, AE does not affect the number and morphology of normal osteoblastic human cells, indicating its selectivity. Moreover, using colorimetric methods, we found that low doses of AE increase the production of osteoblastogenesis markers. These findings indicate that D. dichotoma is a valuable source of bioactive compounds for its regulatory effects on processes involved in metastasis and healthy effects in osteoblasts.
Fil: Lezcano, Virginia Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Mariani, Florencia. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Laboratorio de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; Argentina
Fil: Fernández, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Parodi, Elisa Rosalia. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Laboratorio de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; Argentina
Fil: Morelli, Susana Ana. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Laboratorio de Química Biológica; Argentina
Materia
CYTOTOXICITY
CELL MIGRATION
CELL ADHESION
ALGAL EXTRACT
OSTEOBLASTOGENESIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/217688
Acceder
id CONICETDig_9b0b71748fc324c3da2974ef7e12f589
oai_identifier_str oai:ri.conicet.gov.ar:11336/217688
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Dictyota dichotoma (phaeophyceae) methanolic extracts exhibit antitumoral effects on breast cancer cells and induce osteoblasts differentiationLezcano, Virginia AliciaMariani, FlorenciaFernández, CarolinaParodi, Elisa RosaliaMorelli, Susana AnaCYTOTOXICITYCELL MIGRATIONCELL ADHESIONALGAL EXTRACTOSTEOBLASTOGENESIShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1There is a growing interest in macroalgae as natural products with antioxidant and anticancer activity. In this work, we studied the anti-tumoral effect of an algal extract (AE) derived from the marine alga Dictyota dichotoma on human breast cancer cells (MCF-7). We also evaluated the cytotoxic effects on non-tumorigenic cells and the effects on ostoblastogenesis in vitro. We found that the AE contains high levels of polyphenols and anti-oxidant activity measured by DPPH and Folin-Ciocalteu methods, respectively. Using trypan blue and MTS assays we demonstrated a significant inhibition of MCF-7 cell proliferation and viability. The changes in protein phosphorylation levels were examined through Western blot analysis, finding a decrease of phosphorylated AKT (Ser473) and its target molecule BAD (Ser136). In addition, AE inhibits cell migration determined through the wound healing assay and decreases cellular adhesion at all concentrations probed. Interestingly, AE does not affect the number and morphology of normal osteoblastic human cells, indicating its selectivity. Moreover, using colorimetric methods, we found that low doses of AE increase the production of osteoblastogenesis markers. These findings indicate that D. dichotoma is a valuable source of bioactive compounds for its regulatory effects on processes involved in metastasis and healthy effects in osteoblasts.Fil: Lezcano, Virginia Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Mariani, Florencia. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Laboratorio de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; ArgentinaFil: Fernández, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Parodi, Elisa Rosalia. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Laboratorio de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; ArgentinaFil: Morelli, Susana Ana. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Laboratorio de Química Biológica; ArgentinaAnatolia Academy of sciences2023-02-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/217688Lezcano, Virginia Alicia; Mariani, Florencia; Fernández, Carolina; Parodi, Elisa Rosalia; Morelli, Susana Ana; Dictyota dichotoma (phaeophyceae) methanolic extracts exhibit antitumoral effects on breast cancer cells and induce osteoblasts differentiation; Anatolia Academy of sciences; Journal of Applied Biological Sciences; 17; 2; 9-2-2023; 221-2342146-0108CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.jabsonline.org/index.php/jabs/article/view/972info:eu-repo/semantics/altIdentifier/doi/10.5281/zenodo.8018830info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:07:06Zoai:ri.conicet.gov.ar:11336/217688instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:07:07.149CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Dictyota dichotoma (phaeophyceae) methanolic extracts exhibit antitumoral effects on breast cancer cells and induce osteoblasts differentiation
title Dictyota dichotoma (phaeophyceae) methanolic extracts exhibit antitumoral effects on breast cancer cells and induce osteoblasts differentiation
spellingShingle Dictyota dichotoma (phaeophyceae) methanolic extracts exhibit antitumoral effects on breast cancer cells and induce osteoblasts differentiation
Lezcano, Virginia Alicia
CYTOTOXICITY
CELL MIGRATION
CELL ADHESION
ALGAL EXTRACT
OSTEOBLASTOGENESIS
title_short Dictyota dichotoma (phaeophyceae) methanolic extracts exhibit antitumoral effects on breast cancer cells and induce osteoblasts differentiation
title_full Dictyota dichotoma (phaeophyceae) methanolic extracts exhibit antitumoral effects on breast cancer cells and induce osteoblasts differentiation
title_fullStr Dictyota dichotoma (phaeophyceae) methanolic extracts exhibit antitumoral effects on breast cancer cells and induce osteoblasts differentiation
title_full_unstemmed Dictyota dichotoma (phaeophyceae) methanolic extracts exhibit antitumoral effects on breast cancer cells and induce osteoblasts differentiation
title_sort Dictyota dichotoma (phaeophyceae) methanolic extracts exhibit antitumoral effects on breast cancer cells and induce osteoblasts differentiation
dc.creator.none.fl_str_mv Lezcano, Virginia Alicia
Mariani, Florencia
Fernández, Carolina
Parodi, Elisa Rosalia
Morelli, Susana Ana
author Lezcano, Virginia Alicia
author_facet Lezcano, Virginia Alicia
Mariani, Florencia
Fernández, Carolina
Parodi, Elisa Rosalia
Morelli, Susana Ana
author_role author
author2 Mariani, Florencia
Fernández, Carolina
Parodi, Elisa Rosalia
Morelli, Susana Ana
author2_role author
author
author
author
dc.subject.none.fl_str_mv CYTOTOXICITY
CELL MIGRATION
CELL ADHESION
ALGAL EXTRACT
OSTEOBLASTOGENESIS
topic CYTOTOXICITY
CELL MIGRATION
CELL ADHESION
ALGAL EXTRACT
OSTEOBLASTOGENESIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv There is a growing interest in macroalgae as natural products with antioxidant and anticancer activity. In this work, we studied the anti-tumoral effect of an algal extract (AE) derived from the marine alga Dictyota dichotoma on human breast cancer cells (MCF-7). We also evaluated the cytotoxic effects on non-tumorigenic cells and the effects on ostoblastogenesis in vitro. We found that the AE contains high levels of polyphenols and anti-oxidant activity measured by DPPH and Folin-Ciocalteu methods, respectively. Using trypan blue and MTS assays we demonstrated a significant inhibition of MCF-7 cell proliferation and viability. The changes in protein phosphorylation levels were examined through Western blot analysis, finding a decrease of phosphorylated AKT (Ser473) and its target molecule BAD (Ser136). In addition, AE inhibits cell migration determined through the wound healing assay and decreases cellular adhesion at all concentrations probed. Interestingly, AE does not affect the number and morphology of normal osteoblastic human cells, indicating its selectivity. Moreover, using colorimetric methods, we found that low doses of AE increase the production of osteoblastogenesis markers. These findings indicate that D. dichotoma is a valuable source of bioactive compounds for its regulatory effects on processes involved in metastasis and healthy effects in osteoblasts.
Fil: Lezcano, Virginia Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Mariani, Florencia. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Laboratorio de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; Argentina
Fil: Fernández, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Parodi, Elisa Rosalia. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Laboratorio de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; Argentina
Fil: Morelli, Susana Ana. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Laboratorio de Química Biológica; Argentina
description There is a growing interest in macroalgae as natural products with antioxidant and anticancer activity. In this work, we studied the anti-tumoral effect of an algal extract (AE) derived from the marine alga Dictyota dichotoma on human breast cancer cells (MCF-7). We also evaluated the cytotoxic effects on non-tumorigenic cells and the effects on ostoblastogenesis in vitro. We found that the AE contains high levels of polyphenols and anti-oxidant activity measured by DPPH and Folin-Ciocalteu methods, respectively. Using trypan blue and MTS assays we demonstrated a significant inhibition of MCF-7 cell proliferation and viability. The changes in protein phosphorylation levels were examined through Western blot analysis, finding a decrease of phosphorylated AKT (Ser473) and its target molecule BAD (Ser136). In addition, AE inhibits cell migration determined through the wound healing assay and decreases cellular adhesion at all concentrations probed. Interestingly, AE does not affect the number and morphology of normal osteoblastic human cells, indicating its selectivity. Moreover, using colorimetric methods, we found that low doses of AE increase the production of osteoblastogenesis markers. These findings indicate that D. dichotoma is a valuable source of bioactive compounds for its regulatory effects on processes involved in metastasis and healthy effects in osteoblasts.
publishDate 2023
dc.date.none.fl_str_mv 2023-02-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/217688
Lezcano, Virginia Alicia; Mariani, Florencia; Fernández, Carolina; Parodi, Elisa Rosalia; Morelli, Susana Ana; Dictyota dichotoma (phaeophyceae) methanolic extracts exhibit antitumoral effects on breast cancer cells and induce osteoblasts differentiation; Anatolia Academy of sciences; Journal of Applied Biological Sciences; 17; 2; 9-2-2023; 221-234
2146-0108
CONICET Digital
CONICET
url http://hdl.handle.net/11336/217688
identifier_str_mv Lezcano, Virginia Alicia; Mariani, Florencia; Fernández, Carolina; Parodi, Elisa Rosalia; Morelli, Susana Ana; Dictyota dichotoma (phaeophyceae) methanolic extracts exhibit antitumoral effects on breast cancer cells and induce osteoblasts differentiation; Anatolia Academy of sciences; Journal of Applied Biological Sciences; 17; 2; 9-2-2023; 221-234
2146-0108
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.jabsonline.org/index.php/jabs/article/view/972
info:eu-repo/semantics/altIdentifier/doi/10.5281/zenodo.8018830
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Anatolia Academy of sciences
publisher.none.fl_str_mv Anatolia Academy of sciences
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846781381016813568
score 12.982451

Publicaciones similares