Bordetella bronchiseptica diguanylate cyclase BdcB inhibits the type three secretion system and impacts the immune response

Autores
Belhart, Keila; Sisti, Federico Bernardo; Gestal, Mónica C.; Fernandez, Julieta
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bordetella bronchiseptica is a gram-negative bacterium that causes respiratory diseases in differentanimals, including mice, making B. bronchiseptica the gold-standard model to investigate host?pathogen interaction at the molecular level. B. bronchiseptica utilizes many different mechanisms toprecisely regulate the expression of virulence factors. Cyclic di-GMP is a second messenger synthesizedby diguanylate cyclases and degraded by phosphodiesterases that regulates the expression ofmultiple virulence factors including biofilm formation. As in other bacteria, we have previously shownthat c-di-GMP regulates motility and biofilm formation in B. bronchiseptica. This work describes thediguanylate cyclase BdcB (Bordetella diguanylate cyclase B) as an active diguanylate cyclase thatpromotes biofilm formation and inhibits motility in B. bronchiseptica. The absence of BdcB increasedmacrophage cytotoxicity in vitro and induced a greater production of TNF-α, IL-6, and IL-10 bymacrophages. Our study reveals that BdcB regulates the expression of components of T3SS, animportant virulence factor of B. bronchiseptica. The BbΔbdcB mutant presented increased expressionof T3SS-mediated toxins such as bteA, responsible for cytotoxicity. Our in vivo results revealedthat albeit the absence of bdcB did not affect the ability of B. bronchiseptica to infect and colonizethe respiratory tract of mice, mice infected with BbΔbdcB presented a significantly higher proinflammatoryresponse than those infected with wild type B. bronchiseptica.
Fil: Belhart, Keila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; Argentina
Fil: Sisti, Federico Bernardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; Argentina
Fil: Gestal, Mónica C.. State University of Louisiana; Estados Unidos
Fil: Fernandez, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; Argentina
Materia
Bordetella
C-DI-GMP
Biofilm
Tyope three secretion system
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/230077
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/230077
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Bordetella bronchiseptica diguanylate cyclase BdcB inhibits the type three secretion system and impacts the immune responseBelhart, KeilaSisti, Federico BernardoGestal, Mónica C.Fernandez, JulietaBordetellaC-DI-GMPBiofilmTyope three secretion systemhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Bordetella bronchiseptica is a gram-negative bacterium that causes respiratory diseases in differentanimals, including mice, making B. bronchiseptica the gold-standard model to investigate host?pathogen interaction at the molecular level. B. bronchiseptica utilizes many different mechanisms toprecisely regulate the expression of virulence factors. Cyclic di-GMP is a second messenger synthesizedby diguanylate cyclases and degraded by phosphodiesterases that regulates the expression ofmultiple virulence factors including biofilm formation. As in other bacteria, we have previously shownthat c-di-GMP regulates motility and biofilm formation in B. bronchiseptica. This work describes thediguanylate cyclase BdcB (Bordetella diguanylate cyclase B) as an active diguanylate cyclase thatpromotes biofilm formation and inhibits motility in B. bronchiseptica. The absence of BdcB increasedmacrophage cytotoxicity in vitro and induced a greater production of TNF-α, IL-6, and IL-10 bymacrophages. Our study reveals that BdcB regulates the expression of components of T3SS, animportant virulence factor of B. bronchiseptica. The BbΔbdcB mutant presented increased expressionof T3SS-mediated toxins such as bteA, responsible for cytotoxicity. Our in vivo results revealedthat albeit the absence of bdcB did not affect the ability of B. bronchiseptica to infect and colonizethe respiratory tract of mice, mice infected with BbΔbdcB presented a significantly higher proinflammatoryresponse than those infected with wild type B. bronchiseptica.Fil: Belhart, Keila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; ArgentinaFil: Sisti, Federico Bernardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; ArgentinaFil: Gestal, Mónica C.. State University of Louisiana; Estados UnidosFil: Fernandez, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; ArgentinaNature2023-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/230077Belhart, Keila; Sisti, Federico Bernardo; Gestal, Mónica C.; Fernandez, Julieta; Bordetella bronchiseptica diguanylate cyclase BdcB inhibits the type three secretion system and impacts the immune response; Nature; Scientific Reports; 13; 1; 5-2023; 1-152045-2322CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41598-023-34106-xinfo:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-023-34106-xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:12:52Zoai:ri.conicet.gov.ar:11336/230077instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:12:52.595CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Bordetella bronchiseptica diguanylate cyclase BdcB inhibits the type three secretion system and impacts the immune response
title Bordetella bronchiseptica diguanylate cyclase BdcB inhibits the type three secretion system and impacts the immune response
spellingShingle Bordetella bronchiseptica diguanylate cyclase BdcB inhibits the type three secretion system and impacts the immune response
Belhart, Keila
Bordetella
C-DI-GMP
Biofilm
Tyope three secretion system
title_short Bordetella bronchiseptica diguanylate cyclase BdcB inhibits the type three secretion system and impacts the immune response
title_full Bordetella bronchiseptica diguanylate cyclase BdcB inhibits the type three secretion system and impacts the immune response
title_fullStr Bordetella bronchiseptica diguanylate cyclase BdcB inhibits the type three secretion system and impacts the immune response
title_full_unstemmed Bordetella bronchiseptica diguanylate cyclase BdcB inhibits the type three secretion system and impacts the immune response
title_sort Bordetella bronchiseptica diguanylate cyclase BdcB inhibits the type three secretion system and impacts the immune response
dc.creator.none.fl_str_mv Belhart, Keila
Sisti, Federico Bernardo
Gestal, Mónica C.
Fernandez, Julieta
author Belhart, Keila
author_facet Belhart, Keila
Sisti, Federico Bernardo
Gestal, Mónica C.
Fernandez, Julieta
author_role author
author2 Sisti, Federico Bernardo
Gestal, Mónica C.
Fernandez, Julieta
author2_role author
author
author
dc.subject.none.fl_str_mv Bordetella
C-DI-GMP
Biofilm
Tyope three secretion system
topic Bordetella
C-DI-GMP
Biofilm
Tyope three secretion system
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Bordetella bronchiseptica is a gram-negative bacterium that causes respiratory diseases in differentanimals, including mice, making B. bronchiseptica the gold-standard model to investigate host?pathogen interaction at the molecular level. B. bronchiseptica utilizes many different mechanisms toprecisely regulate the expression of virulence factors. Cyclic di-GMP is a second messenger synthesizedby diguanylate cyclases and degraded by phosphodiesterases that regulates the expression ofmultiple virulence factors including biofilm formation. As in other bacteria, we have previously shownthat c-di-GMP regulates motility and biofilm formation in B. bronchiseptica. This work describes thediguanylate cyclase BdcB (Bordetella diguanylate cyclase B) as an active diguanylate cyclase thatpromotes biofilm formation and inhibits motility in B. bronchiseptica. The absence of BdcB increasedmacrophage cytotoxicity in vitro and induced a greater production of TNF-α, IL-6, and IL-10 bymacrophages. Our study reveals that BdcB regulates the expression of components of T3SS, animportant virulence factor of B. bronchiseptica. The BbΔbdcB mutant presented increased expressionof T3SS-mediated toxins such as bteA, responsible for cytotoxicity. Our in vivo results revealedthat albeit the absence of bdcB did not affect the ability of B. bronchiseptica to infect and colonizethe respiratory tract of mice, mice infected with BbΔbdcB presented a significantly higher proinflammatoryresponse than those infected with wild type B. bronchiseptica.
Fil: Belhart, Keila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; Argentina
Fil: Sisti, Federico Bernardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; Argentina
Fil: Gestal, Mónica C.. State University of Louisiana; Estados Unidos
Fil: Fernandez, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; Argentina
description Bordetella bronchiseptica is a gram-negative bacterium that causes respiratory diseases in differentanimals, including mice, making B. bronchiseptica the gold-standard model to investigate host?pathogen interaction at the molecular level. B. bronchiseptica utilizes many different mechanisms toprecisely regulate the expression of virulence factors. Cyclic di-GMP is a second messenger synthesizedby diguanylate cyclases and degraded by phosphodiesterases that regulates the expression ofmultiple virulence factors including biofilm formation. As in other bacteria, we have previously shownthat c-di-GMP regulates motility and biofilm formation in B. bronchiseptica. This work describes thediguanylate cyclase BdcB (Bordetella diguanylate cyclase B) as an active diguanylate cyclase thatpromotes biofilm formation and inhibits motility in B. bronchiseptica. The absence of BdcB increasedmacrophage cytotoxicity in vitro and induced a greater production of TNF-α, IL-6, and IL-10 bymacrophages. Our study reveals that BdcB regulates the expression of components of T3SS, animportant virulence factor of B. bronchiseptica. The BbΔbdcB mutant presented increased expressionof T3SS-mediated toxins such as bteA, responsible for cytotoxicity. Our in vivo results revealedthat albeit the absence of bdcB did not affect the ability of B. bronchiseptica to infect and colonizethe respiratory tract of mice, mice infected with BbΔbdcB presented a significantly higher proinflammatoryresponse than those infected with wild type B. bronchiseptica.
publishDate 2023
dc.date.none.fl_str_mv 2023-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/230077
Belhart, Keila; Sisti, Federico Bernardo; Gestal, Mónica C.; Fernandez, Julieta; Bordetella bronchiseptica diguanylate cyclase BdcB inhibits the type three secretion system and impacts the immune response; Nature; Scientific Reports; 13; 1; 5-2023; 1-15
2045-2322
CONICET Digital
CONICET
url http://hdl.handle.net/11336/230077
identifier_str_mv Belhart, Keila; Sisti, Federico Bernardo; Gestal, Mónica C.; Fernandez, Julieta; Bordetella bronchiseptica diguanylate cyclase BdcB inhibits the type three secretion system and impacts the immune response; Nature; Scientific Reports; 13; 1; 5-2023; 1-15
2045-2322
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-023-34106-x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Nature
publisher.none.fl_str_mv Nature
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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