Bordetella bronchiseptica diguanylate cyclase BdcB inhibits the type three secretion system and impacts the immune response
- Autores
- Belhart, Keila; Sisti, Federico Bernardo; Gestal, Mónica C.; Fernandez, Julieta
- Año de publicación
- 2023
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Bordetella bronchiseptica is a gram-negative bacterium that causes respiratory diseases in differentanimals, including mice, making B. bronchiseptica the gold-standard model to investigate host?pathogen interaction at the molecular level. B. bronchiseptica utilizes many different mechanisms toprecisely regulate the expression of virulence factors. Cyclic di-GMP is a second messenger synthesizedby diguanylate cyclases and degraded by phosphodiesterases that regulates the expression ofmultiple virulence factors including biofilm formation. As in other bacteria, we have previously shownthat c-di-GMP regulates motility and biofilm formation in B. bronchiseptica. This work describes thediguanylate cyclase BdcB (Bordetella diguanylate cyclase B) as an active diguanylate cyclase thatpromotes biofilm formation and inhibits motility in B. bronchiseptica. The absence of BdcB increasedmacrophage cytotoxicity in vitro and induced a greater production of TNF-α, IL-6, and IL-10 bymacrophages. Our study reveals that BdcB regulates the expression of components of T3SS, animportant virulence factor of B. bronchiseptica. The BbΔbdcB mutant presented increased expressionof T3SS-mediated toxins such as bteA, responsible for cytotoxicity. Our in vivo results revealedthat albeit the absence of bdcB did not affect the ability of B. bronchiseptica to infect and colonizethe respiratory tract of mice, mice infected with BbΔbdcB presented a significantly higher proinflammatoryresponse than those infected with wild type B. bronchiseptica.
Fil: Belhart, Keila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; Argentina
Fil: Sisti, Federico Bernardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; Argentina
Fil: Gestal, Mónica C.. State University of Louisiana; Estados Unidos
Fil: Fernandez, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; Argentina - Materia
-
Bordetella
C-DI-GMP
Biofilm
Tyope three secretion system - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/230077
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Bordetella bronchiseptica diguanylate cyclase BdcB inhibits the type three secretion system and impacts the immune responseBelhart, KeilaSisti, Federico BernardoGestal, Mónica C.Fernandez, JulietaBordetellaC-DI-GMPBiofilmTyope three secretion systemhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Bordetella bronchiseptica is a gram-negative bacterium that causes respiratory diseases in differentanimals, including mice, making B. bronchiseptica the gold-standard model to investigate host?pathogen interaction at the molecular level. B. bronchiseptica utilizes many different mechanisms toprecisely regulate the expression of virulence factors. Cyclic di-GMP is a second messenger synthesizedby diguanylate cyclases and degraded by phosphodiesterases that regulates the expression ofmultiple virulence factors including biofilm formation. As in other bacteria, we have previously shownthat c-di-GMP regulates motility and biofilm formation in B. bronchiseptica. This work describes thediguanylate cyclase BdcB (Bordetella diguanylate cyclase B) as an active diguanylate cyclase thatpromotes biofilm formation and inhibits motility in B. bronchiseptica. The absence of BdcB increasedmacrophage cytotoxicity in vitro and induced a greater production of TNF-α, IL-6, and IL-10 bymacrophages. Our study reveals that BdcB regulates the expression of components of T3SS, animportant virulence factor of B. bronchiseptica. The BbΔbdcB mutant presented increased expressionof T3SS-mediated toxins such as bteA, responsible for cytotoxicity. Our in vivo results revealedthat albeit the absence of bdcB did not affect the ability of B. bronchiseptica to infect and colonizethe respiratory tract of mice, mice infected with BbΔbdcB presented a significantly higher proinflammatoryresponse than those infected with wild type B. bronchiseptica.Fil: Belhart, Keila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; ArgentinaFil: Sisti, Federico Bernardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; ArgentinaFil: Gestal, Mónica C.. State University of Louisiana; Estados UnidosFil: Fernandez, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; ArgentinaNature2023-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/230077Belhart, Keila; Sisti, Federico Bernardo; Gestal, Mónica C.; Fernandez, Julieta; Bordetella bronchiseptica diguanylate cyclase BdcB inhibits the type three secretion system and impacts the immune response; Nature; Scientific Reports; 13; 1; 5-2023; 1-152045-2322CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41598-023-34106-xinfo:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-023-34106-xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:40:26Zoai:ri.conicet.gov.ar:11336/230077instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:40:26.377CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Bordetella bronchiseptica diguanylate cyclase BdcB inhibits the type three secretion system and impacts the immune response |
title |
Bordetella bronchiseptica diguanylate cyclase BdcB inhibits the type three secretion system and impacts the immune response |
spellingShingle |
Bordetella bronchiseptica diguanylate cyclase BdcB inhibits the type three secretion system and impacts the immune response Belhart, Keila Bordetella C-DI-GMP Biofilm Tyope three secretion system |
title_short |
Bordetella bronchiseptica diguanylate cyclase BdcB inhibits the type three secretion system and impacts the immune response |
title_full |
Bordetella bronchiseptica diguanylate cyclase BdcB inhibits the type three secretion system and impacts the immune response |
title_fullStr |
Bordetella bronchiseptica diguanylate cyclase BdcB inhibits the type three secretion system and impacts the immune response |
title_full_unstemmed |
Bordetella bronchiseptica diguanylate cyclase BdcB inhibits the type three secretion system and impacts the immune response |
title_sort |
Bordetella bronchiseptica diguanylate cyclase BdcB inhibits the type three secretion system and impacts the immune response |
dc.creator.none.fl_str_mv |
Belhart, Keila Sisti, Federico Bernardo Gestal, Mónica C. Fernandez, Julieta |
author |
Belhart, Keila |
author_facet |
Belhart, Keila Sisti, Federico Bernardo Gestal, Mónica C. Fernandez, Julieta |
author_role |
author |
author2 |
Sisti, Federico Bernardo Gestal, Mónica C. Fernandez, Julieta |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
Bordetella C-DI-GMP Biofilm Tyope three secretion system |
topic |
Bordetella C-DI-GMP Biofilm Tyope three secretion system |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Bordetella bronchiseptica is a gram-negative bacterium that causes respiratory diseases in differentanimals, including mice, making B. bronchiseptica the gold-standard model to investigate host?pathogen interaction at the molecular level. B. bronchiseptica utilizes many different mechanisms toprecisely regulate the expression of virulence factors. Cyclic di-GMP is a second messenger synthesizedby diguanylate cyclases and degraded by phosphodiesterases that regulates the expression ofmultiple virulence factors including biofilm formation. As in other bacteria, we have previously shownthat c-di-GMP regulates motility and biofilm formation in B. bronchiseptica. This work describes thediguanylate cyclase BdcB (Bordetella diguanylate cyclase B) as an active diguanylate cyclase thatpromotes biofilm formation and inhibits motility in B. bronchiseptica. The absence of BdcB increasedmacrophage cytotoxicity in vitro and induced a greater production of TNF-α, IL-6, and IL-10 bymacrophages. Our study reveals that BdcB regulates the expression of components of T3SS, animportant virulence factor of B. bronchiseptica. The BbΔbdcB mutant presented increased expressionof T3SS-mediated toxins such as bteA, responsible for cytotoxicity. Our in vivo results revealedthat albeit the absence of bdcB did not affect the ability of B. bronchiseptica to infect and colonizethe respiratory tract of mice, mice infected with BbΔbdcB presented a significantly higher proinflammatoryresponse than those infected with wild type B. bronchiseptica. Fil: Belhart, Keila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; Argentina Fil: Sisti, Federico Bernardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; Argentina Fil: Gestal, Mónica C.. State University of Louisiana; Estados Unidos Fil: Fernandez, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas; Argentina |
description |
Bordetella bronchiseptica is a gram-negative bacterium that causes respiratory diseases in differentanimals, including mice, making B. bronchiseptica the gold-standard model to investigate host?pathogen interaction at the molecular level. B. bronchiseptica utilizes many different mechanisms toprecisely regulate the expression of virulence factors. Cyclic di-GMP is a second messenger synthesizedby diguanylate cyclases and degraded by phosphodiesterases that regulates the expression ofmultiple virulence factors including biofilm formation. As in other bacteria, we have previously shownthat c-di-GMP regulates motility and biofilm formation in B. bronchiseptica. This work describes thediguanylate cyclase BdcB (Bordetella diguanylate cyclase B) as an active diguanylate cyclase thatpromotes biofilm formation and inhibits motility in B. bronchiseptica. The absence of BdcB increasedmacrophage cytotoxicity in vitro and induced a greater production of TNF-α, IL-6, and IL-10 bymacrophages. Our study reveals that BdcB regulates the expression of components of T3SS, animportant virulence factor of B. bronchiseptica. The BbΔbdcB mutant presented increased expressionof T3SS-mediated toxins such as bteA, responsible for cytotoxicity. Our in vivo results revealedthat albeit the absence of bdcB did not affect the ability of B. bronchiseptica to infect and colonizethe respiratory tract of mice, mice infected with BbΔbdcB presented a significantly higher proinflammatoryresponse than those infected with wild type B. bronchiseptica. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-05 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/230077 Belhart, Keila; Sisti, Federico Bernardo; Gestal, Mónica C.; Fernandez, Julieta; Bordetella bronchiseptica diguanylate cyclase BdcB inhibits the type three secretion system and impacts the immune response; Nature; Scientific Reports; 13; 1; 5-2023; 1-15 2045-2322 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/230077 |
identifier_str_mv |
Belhart, Keila; Sisti, Federico Bernardo; Gestal, Mónica C.; Fernandez, Julieta; Bordetella bronchiseptica diguanylate cyclase BdcB inhibits the type three secretion system and impacts the immune response; Nature; Scientific Reports; 13; 1; 5-2023; 1-15 2045-2322 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41598-023-34106-x info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-023-34106-x |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/ |
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openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/2.5/ar/ |
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application/pdf application/pdf application/pdf application/pdf |
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Nature |
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Nature |
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reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.070432 |