Bordetella bronchiseptica diguanylate cyclase BdcB inhibits the type three secretion system and impacts the immune response
- Autores
- Belhart, Keila; Sisti, Federico; Gestal, Mónica C.; Fernández, Julieta
- Año de publicación
- 2023
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Bordetella bronchiseptica is a gram-negative bacterium that causes respiratory diseases in diferent animals, including mice, making B. bronchiseptica the gold-standard model to investigate host– pathogen interaction at the molecular level. B. bronchiseptica utilizes many diferent mechanisms to precisely regulate the expression of virulence factors. Cyclic di-GMP is a second messenger synthesized by diguanylate cyclases and degraded by phosphodiesterases that regulates the expression of multiple virulence factors including bioflm formation. As in other bacteria, we have previously shown that c-di-GMP regulates motility and bioflm formation in B. bronchiseptica. This work describes the diguanylate cyclase BdcB (Bordetella diguanylate cyclase B) as an active diguanylate cyclase that promotes bioflm formation and inhibits motility in B. bronchiseptica. The absence of BdcB increased macrophage cytotoxicity in vitro and induced a greater production of TNF-α, IL-6, and IL-10 by macrophages. Our study reveals that BdcB regulates the expression of components of T3SS, an important virulence factor of B. bronchiseptica. The Bb∆bdcB mutant presented increased expression of T3SS-mediated toxins such as bteA, responsible for cytotoxicity. Our in vivo results revealed that albeit the absence of bdcB did not afect the ability of B. bronchiseptica to infect and colonize the respiratory tract of mice, mice infected with Bb∆bdcB presented a signifcantly higher proinfammatory response than those infected with wild type B. bronchiseptica.
Instituto de Biotecnología y Biología Molecular - Materia
-
Biología
Inmunología
Microbiología
Biología molecular
patogénesis - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by/4.0/
- Repositorio
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/160042
Ver los metadatos del registro completo
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Bordetella bronchiseptica diguanylate cyclase BdcB inhibits the type three secretion system and impacts the immune responseBelhart, KeilaSisti, FedericoGestal, Mónica C.Fernández, JulietaBiologíaInmunologíaMicrobiologíaBiología molecularpatogénesisBordetella bronchiseptica is a gram-negative bacterium that causes respiratory diseases in diferent animals, including mice, making B. bronchiseptica the gold-standard model to investigate host– pathogen interaction at the molecular level. B. bronchiseptica utilizes many diferent mechanisms to precisely regulate the expression of virulence factors. Cyclic di-GMP is a second messenger synthesized by diguanylate cyclases and degraded by phosphodiesterases that regulates the expression of multiple virulence factors including bioflm formation. As in other bacteria, we have previously shown that c-di-GMP regulates motility and bioflm formation in B. bronchiseptica. This work describes the diguanylate cyclase BdcB (Bordetella diguanylate cyclase B) as an active diguanylate cyclase that promotes bioflm formation and inhibits motility in B. bronchiseptica. The absence of BdcB increased macrophage cytotoxicity in vitro and induced a greater production of TNF-α, IL-6, and IL-10 by macrophages. Our study reveals that BdcB regulates the expression of components of T3SS, an important virulence factor of B. bronchiseptica. The Bb∆bdcB mutant presented increased expression of T3SS-mediated toxins such as bteA, responsible for cytotoxicity. Our in vivo results revealed that albeit the absence of bdcB did not afect the ability of B. bronchiseptica to infect and colonize the respiratory tract of mice, mice infected with Bb∆bdcB presented a signifcantly higher proinfammatory response than those infected with wild type B. bronchiseptica.Instituto de Biotecnología y Biología Molecular2023-05-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/160042enginfo:eu-repo/semantics/altIdentifier/issn/2045-2322info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-023-34106-xinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:41:52Zoai:sedici.unlp.edu.ar:10915/160042Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:41:52.46SEDICI (UNLP) - Universidad Nacional de La Platafalse |
dc.title.none.fl_str_mv |
Bordetella bronchiseptica diguanylate cyclase BdcB inhibits the type three secretion system and impacts the immune response |
title |
Bordetella bronchiseptica diguanylate cyclase BdcB inhibits the type three secretion system and impacts the immune response |
spellingShingle |
Bordetella bronchiseptica diguanylate cyclase BdcB inhibits the type three secretion system and impacts the immune response Belhart, Keila Biología Inmunología Microbiología Biología molecular patogénesis |
title_short |
Bordetella bronchiseptica diguanylate cyclase BdcB inhibits the type three secretion system and impacts the immune response |
title_full |
Bordetella bronchiseptica diguanylate cyclase BdcB inhibits the type three secretion system and impacts the immune response |
title_fullStr |
Bordetella bronchiseptica diguanylate cyclase BdcB inhibits the type three secretion system and impacts the immune response |
title_full_unstemmed |
Bordetella bronchiseptica diguanylate cyclase BdcB inhibits the type three secretion system and impacts the immune response |
title_sort |
Bordetella bronchiseptica diguanylate cyclase BdcB inhibits the type three secretion system and impacts the immune response |
dc.creator.none.fl_str_mv |
Belhart, Keila Sisti, Federico Gestal, Mónica C. Fernández, Julieta |
author |
Belhart, Keila |
author_facet |
Belhart, Keila Sisti, Federico Gestal, Mónica C. Fernández, Julieta |
author_role |
author |
author2 |
Sisti, Federico Gestal, Mónica C. Fernández, Julieta |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
Biología Inmunología Microbiología Biología molecular patogénesis |
topic |
Biología Inmunología Microbiología Biología molecular patogénesis |
dc.description.none.fl_txt_mv |
Bordetella bronchiseptica is a gram-negative bacterium that causes respiratory diseases in diferent animals, including mice, making B. bronchiseptica the gold-standard model to investigate host– pathogen interaction at the molecular level. B. bronchiseptica utilizes many diferent mechanisms to precisely regulate the expression of virulence factors. Cyclic di-GMP is a second messenger synthesized by diguanylate cyclases and degraded by phosphodiesterases that regulates the expression of multiple virulence factors including bioflm formation. As in other bacteria, we have previously shown that c-di-GMP regulates motility and bioflm formation in B. bronchiseptica. This work describes the diguanylate cyclase BdcB (Bordetella diguanylate cyclase B) as an active diguanylate cyclase that promotes bioflm formation and inhibits motility in B. bronchiseptica. The absence of BdcB increased macrophage cytotoxicity in vitro and induced a greater production of TNF-α, IL-6, and IL-10 by macrophages. Our study reveals that BdcB regulates the expression of components of T3SS, an important virulence factor of B. bronchiseptica. The Bb∆bdcB mutant presented increased expression of T3SS-mediated toxins such as bteA, responsible for cytotoxicity. Our in vivo results revealed that albeit the absence of bdcB did not afect the ability of B. bronchiseptica to infect and colonize the respiratory tract of mice, mice infected with Bb∆bdcB presented a signifcantly higher proinfammatory response than those infected with wild type B. bronchiseptica. Instituto de Biotecnología y Biología Molecular |
description |
Bordetella bronchiseptica is a gram-negative bacterium that causes respiratory diseases in diferent animals, including mice, making B. bronchiseptica the gold-standard model to investigate host– pathogen interaction at the molecular level. B. bronchiseptica utilizes many diferent mechanisms to precisely regulate the expression of virulence factors. Cyclic di-GMP is a second messenger synthesized by diguanylate cyclases and degraded by phosphodiesterases that regulates the expression of multiple virulence factors including bioflm formation. As in other bacteria, we have previously shown that c-di-GMP regulates motility and bioflm formation in B. bronchiseptica. This work describes the diguanylate cyclase BdcB (Bordetella diguanylate cyclase B) as an active diguanylate cyclase that promotes bioflm formation and inhibits motility in B. bronchiseptica. The absence of BdcB increased macrophage cytotoxicity in vitro and induced a greater production of TNF-α, IL-6, and IL-10 by macrophages. Our study reveals that BdcB regulates the expression of components of T3SS, an important virulence factor of B. bronchiseptica. The Bb∆bdcB mutant presented increased expression of T3SS-mediated toxins such as bteA, responsible for cytotoxicity. Our in vivo results revealed that albeit the absence of bdcB did not afect the ability of B. bronchiseptica to infect and colonize the respiratory tract of mice, mice infected with Bb∆bdcB presented a signifcantly higher proinfammatory response than those infected with wild type B. bronchiseptica. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-05-02 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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http://sedici.unlp.edu.ar/handle/10915/160042 |
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eng |
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eng |
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