LuMPIS - A modified luminescence-based mammalian interactome mapping pull-down assay for the investigation of protein-protein interactions encoded by GC-low ORFs

Autores
Vizoso Pinto, María Guadalupe; Villegas, Josefina Maria; Peter, Jan; Haase, Rudolf; Haas, Jürgen; Lotz, Amelie Sophia; Muntau, Ania Carolina; Baiker, Armin
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The GC content is highly variable among the genomes of different organisms. It has been shown that recombinant gene expression in mammalian cells is much more efficient when GC-rich coding sequences of a certain protein are used. In order to study protein-protein interactions in Varicella zoster virus, a GC-low herpesvirus, we have developed a novel luminescence-based maltose-binding protein pull-down interaction screening system (LuMPIS) that is able to overcome the impaired protein expression levels of GC-low ORFs in mammalian expression systems. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.
Fil: Vizoso Pinto, María Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Ludwig Maximilians Universitat; Alemania
Fil: Villegas, Josefina Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Ludwig Maximilians Universitat; Alemania
Fil: Peter, Jan. Ludwig Maximilians Universitat; Alemania
Fil: Haase, Rudolf. Ludwig Maximilians Universitat; Alemania
Fil: Haas, Jürgen. Ludwig Maximilians Universitat; Alemania. University of Edinburgh; Reino Unido
Fil: Lotz, Amelie Sophia. Ludwig Maximilians Universitat; Alemania
Fil: Muntau, Ania Carolina. Ludwig Maximilians Universitat; Alemania
Fil: Baiker, Armin. Ludwig Maximilians Universitat; Alemania
Materia
Peptide Sequence Tag
Protein Expression
Protein-Protein Interaction
Proteomics Methods
System Biology
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/65702
Acceder
id CONICETDig_9a949221da21f7c9c5ad622c1434eb38
oai_identifier_str oai:ri.conicet.gov.ar:11336/65702
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling LuMPIS - A modified luminescence-based mammalian interactome mapping pull-down assay for the investigation of protein-protein interactions encoded by GC-low ORFsVizoso Pinto, María GuadalupeVillegas, Josefina MariaPeter, JanHaase, RudolfHaas, JürgenLotz, Amelie SophiaMuntau, Ania CarolinaBaiker, ArminPeptide Sequence TagProtein ExpressionProtein-Protein InteractionProteomics MethodsSystem Biologyhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The GC content is highly variable among the genomes of different organisms. It has been shown that recombinant gene expression in mammalian cells is much more efficient when GC-rich coding sequences of a certain protein are used. In order to study protein-protein interactions in Varicella zoster virus, a GC-low herpesvirus, we have developed a novel luminescence-based maltose-binding protein pull-down interaction screening system (LuMPIS) that is able to overcome the impaired protein expression levels of GC-low ORFs in mammalian expression systems. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.Fil: Vizoso Pinto, María Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Ludwig Maximilians Universitat; AlemaniaFil: Villegas, Josefina Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Ludwig Maximilians Universitat; AlemaniaFil: Peter, Jan. Ludwig Maximilians Universitat; AlemaniaFil: Haase, Rudolf. Ludwig Maximilians Universitat; AlemaniaFil: Haas, Jürgen. Ludwig Maximilians Universitat; Alemania. University of Edinburgh; Reino UnidoFil: Lotz, Amelie Sophia. Ludwig Maximilians Universitat; AlemaniaFil: Muntau, Ania Carolina. Ludwig Maximilians Universitat; AlemaniaFil: Baiker, Armin. Ludwig Maximilians Universitat; AlemaniaWiley VCH Verlag2009-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/65702Vizoso Pinto, María Guadalupe; Villegas, Josefina Maria; Peter, Jan; Haase, Rudolf; Haas, Jürgen; et al.; LuMPIS - A modified luminescence-based mammalian interactome mapping pull-down assay for the investigation of protein-protein interactions encoded by GC-low ORFs; Wiley VCH Verlag; Proteomics (weinheim. Print); 9; 23; 12-2009; 5303-53081615-9853CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/pmic.200900298info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/pmic.200900298info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:26:31Zoai:ri.conicet.gov.ar:11336/65702instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:26:31.737CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv LuMPIS - A modified luminescence-based mammalian interactome mapping pull-down assay for the investigation of protein-protein interactions encoded by GC-low ORFs
title LuMPIS - A modified luminescence-based mammalian interactome mapping pull-down assay for the investigation of protein-protein interactions encoded by GC-low ORFs
spellingShingle LuMPIS - A modified luminescence-based mammalian interactome mapping pull-down assay for the investigation of protein-protein interactions encoded by GC-low ORFs
Vizoso Pinto, María Guadalupe
Peptide Sequence Tag
Protein Expression
Protein-Protein Interaction
Proteomics Methods
System Biology
title_short LuMPIS - A modified luminescence-based mammalian interactome mapping pull-down assay for the investigation of protein-protein interactions encoded by GC-low ORFs
title_full LuMPIS - A modified luminescence-based mammalian interactome mapping pull-down assay for the investigation of protein-protein interactions encoded by GC-low ORFs
title_fullStr LuMPIS - A modified luminescence-based mammalian interactome mapping pull-down assay for the investigation of protein-protein interactions encoded by GC-low ORFs
title_full_unstemmed LuMPIS - A modified luminescence-based mammalian interactome mapping pull-down assay for the investigation of protein-protein interactions encoded by GC-low ORFs
title_sort LuMPIS - A modified luminescence-based mammalian interactome mapping pull-down assay for the investigation of protein-protein interactions encoded by GC-low ORFs
dc.creator.none.fl_str_mv Vizoso Pinto, María Guadalupe
Villegas, Josefina Maria
Peter, Jan
Haase, Rudolf
Haas, Jürgen
Lotz, Amelie Sophia
Muntau, Ania Carolina
Baiker, Armin
author Vizoso Pinto, María Guadalupe
author_facet Vizoso Pinto, María Guadalupe
Villegas, Josefina Maria
Peter, Jan
Haase, Rudolf
Haas, Jürgen
Lotz, Amelie Sophia
Muntau, Ania Carolina
Baiker, Armin
author_role author
author2 Villegas, Josefina Maria
Peter, Jan
Haase, Rudolf
Haas, Jürgen
Lotz, Amelie Sophia
Muntau, Ania Carolina
Baiker, Armin
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Peptide Sequence Tag
Protein Expression
Protein-Protein Interaction
Proteomics Methods
System Biology
topic Peptide Sequence Tag
Protein Expression
Protein-Protein Interaction
Proteomics Methods
System Biology
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The GC content is highly variable among the genomes of different organisms. It has been shown that recombinant gene expression in mammalian cells is much more efficient when GC-rich coding sequences of a certain protein are used. In order to study protein-protein interactions in Varicella zoster virus, a GC-low herpesvirus, we have developed a novel luminescence-based maltose-binding protein pull-down interaction screening system (LuMPIS) that is able to overcome the impaired protein expression levels of GC-low ORFs in mammalian expression systems. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.
Fil: Vizoso Pinto, María Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Ludwig Maximilians Universitat; Alemania
Fil: Villegas, Josefina Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Ludwig Maximilians Universitat; Alemania
Fil: Peter, Jan. Ludwig Maximilians Universitat; Alemania
Fil: Haase, Rudolf. Ludwig Maximilians Universitat; Alemania
Fil: Haas, Jürgen. Ludwig Maximilians Universitat; Alemania. University of Edinburgh; Reino Unido
Fil: Lotz, Amelie Sophia. Ludwig Maximilians Universitat; Alemania
Fil: Muntau, Ania Carolina. Ludwig Maximilians Universitat; Alemania
Fil: Baiker, Armin. Ludwig Maximilians Universitat; Alemania
description The GC content is highly variable among the genomes of different organisms. It has been shown that recombinant gene expression in mammalian cells is much more efficient when GC-rich coding sequences of a certain protein are used. In order to study protein-protein interactions in Varicella zoster virus, a GC-low herpesvirus, we have developed a novel luminescence-based maltose-binding protein pull-down interaction screening system (LuMPIS) that is able to overcome the impaired protein expression levels of GC-low ORFs in mammalian expression systems. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.
publishDate 2009
dc.date.none.fl_str_mv 2009-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/65702
Vizoso Pinto, María Guadalupe; Villegas, Josefina Maria; Peter, Jan; Haase, Rudolf; Haas, Jürgen; et al.; LuMPIS - A modified luminescence-based mammalian interactome mapping pull-down assay for the investigation of protein-protein interactions encoded by GC-low ORFs; Wiley VCH Verlag; Proteomics (weinheim. Print); 9; 23; 12-2009; 5303-5308
1615-9853
CONICET Digital
CONICET
url http://hdl.handle.net/11336/65702
identifier_str_mv Vizoso Pinto, María Guadalupe; Villegas, Josefina Maria; Peter, Jan; Haase, Rudolf; Haas, Jürgen; et al.; LuMPIS - A modified luminescence-based mammalian interactome mapping pull-down assay for the investigation of protein-protein interactions encoded by GC-low ORFs; Wiley VCH Verlag; Proteomics (weinheim. Print); 9; 23; 12-2009; 5303-5308
1615-9853
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/pmic.200900298
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/pmic.200900298
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley VCH Verlag
publisher.none.fl_str_mv Wiley VCH Verlag
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614266754170880
score 13.070432

Publicaciones similares