Unambiguous Determination of Benzo[a]pyrene and Dibenzo[a,l]pyrene in HPLC Fractions via Room-Temperature Fluorescence Excitation–Emission Matrices

Autores
Knecht, George T.; Nauth, Stephanie D.; Gomez Alvarado, Juan C.; Santana, Anthony M.; Goicoechea, Hector Casimiro; Campiglia, Andres D.
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
When high-performance liquid chromatography (HPLC) is used for the analysis of polycyclic aromatic hydrocarbons (PAHs) in complex samples, further examination of HPLC fractions is recommended to confirm PAH assignments solely based on retention times. Gas chromatography–mass spectrometry (GC-MS) has been particularly relevant in the unambiguous determination of PAHs with remarkably similar retention times. The combination of HPLC and GC requires lengthy analysis times to ensure proper assignments. This article presents an approach for the analysis of co-eluted PAHs with no need for further chromatographic separation. Benzo[a]pyrene (BaP) and dibenzo[a,l]pyrene (DBalP) were directly determined in a co-eluted HPLC fraction via room-temperature fluorescence excitation–emission matrices (RTF-EEMs). RTF-EEMs can be recorded in a matter of seconds with a spectrofluorometer equipped with a multichannel detection system. The spectral overlapping of BaP and DBalP was resolved using parallel factor analysis (PARAFAC). The analytical advantages of this approach were demonstrated with the trace analysis (ng/mL) of these two PAHs in pre-concentrated tobacco extracts.
Fil: Knecht, George T.. University of Florida; Estados Unidos
Fil: Nauth, Stephanie D.. University of Florida; Estados Unidos
Fil: Gomez Alvarado, Juan C.. University of Florida; Estados Unidos
Fil: Santana, Anthony M.. University of Florida; Estados Unidos
Fil: Goicoechea, Hector Casimiro. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Campiglia, Andres D.. University of Florida; Estados Unidos
Materia
dibenzo[a,l]pyrene
HPLC
room-temperature fluorescence
PARAFAC;
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/276456
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Unambiguous Determination of Benzo[a]pyrene and Dibenzo[a,l]pyrene in HPLC Fractions via Room-Temperature Fluorescence Excitation–Emission MatricesKnecht, George T.Nauth, Stephanie D.Gomez Alvarado, Juan C.Santana, Anthony M.Goicoechea, Hector CasimiroCampiglia, Andres D.dibenzo[a,l]pyreneHPLCroom-temperature fluorescencePARAFAC;https://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1When high-performance liquid chromatography (HPLC) is used for the analysis of polycyclic aromatic hydrocarbons (PAHs) in complex samples, further examination of HPLC fractions is recommended to confirm PAH assignments solely based on retention times. Gas chromatography–mass spectrometry (GC-MS) has been particularly relevant in the unambiguous determination of PAHs with remarkably similar retention times. The combination of HPLC and GC requires lengthy analysis times to ensure proper assignments. This article presents an approach for the analysis of co-eluted PAHs with no need for further chromatographic separation. Benzo[a]pyrene (BaP) and dibenzo[a,l]pyrene (DBalP) were directly determined in a co-eluted HPLC fraction via room-temperature fluorescence excitation–emission matrices (RTF-EEMs). RTF-EEMs can be recorded in a matter of seconds with a spectrofluorometer equipped with a multichannel detection system. The spectral overlapping of BaP and DBalP was resolved using parallel factor analysis (PARAFAC). The analytical advantages of this approach were demonstrated with the trace analysis (ng/mL) of these two PAHs in pre-concentrated tobacco extracts.Fil: Knecht, George T.. University of Florida; Estados UnidosFil: Nauth, Stephanie D.. University of Florida; Estados UnidosFil: Gomez Alvarado, Juan C.. University of Florida; Estados UnidosFil: Santana, Anthony M.. University of Florida; Estados UnidosFil: Goicoechea, Hector Casimiro. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Campiglia, Andres D.. University of Florida; Estados UnidosMolecular Diversity Preservation International2025-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/276456Knecht, George T.; Nauth, Stephanie D.; Gomez Alvarado, Juan C.; Santana, Anthony M.; Goicoechea, Hector Casimiro; et al.; Unambiguous Determination of Benzo[a]pyrene and Dibenzo[a,l]pyrene in HPLC Fractions via Room-Temperature Fluorescence Excitation–Emission Matrices; Molecular Diversity Preservation International; Molecules; 30; 7; 3-2025; 1-111420-3049CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1420-3049/30/7/1550info:eu-repo/semantics/altIdentifier/doi/10.3390/molecules30071550info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-12-17T15:00:52Zoai:ri.conicet.gov.ar:11336/276456instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-12-17 15:00:52.858CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Unambiguous Determination of Benzo[a]pyrene and Dibenzo[a,l]pyrene in HPLC Fractions via Room-Temperature Fluorescence Excitation–Emission Matrices
title Unambiguous Determination of Benzo[a]pyrene and Dibenzo[a,l]pyrene in HPLC Fractions via Room-Temperature Fluorescence Excitation–Emission Matrices
spellingShingle Unambiguous Determination of Benzo[a]pyrene and Dibenzo[a,l]pyrene in HPLC Fractions via Room-Temperature Fluorescence Excitation–Emission Matrices
Knecht, George T.
dibenzo[a,l]pyrene
HPLC
room-temperature fluorescence
PARAFAC;
title_short Unambiguous Determination of Benzo[a]pyrene and Dibenzo[a,l]pyrene in HPLC Fractions via Room-Temperature Fluorescence Excitation–Emission Matrices
title_full Unambiguous Determination of Benzo[a]pyrene and Dibenzo[a,l]pyrene in HPLC Fractions via Room-Temperature Fluorescence Excitation–Emission Matrices
title_fullStr Unambiguous Determination of Benzo[a]pyrene and Dibenzo[a,l]pyrene in HPLC Fractions via Room-Temperature Fluorescence Excitation–Emission Matrices
title_full_unstemmed Unambiguous Determination of Benzo[a]pyrene and Dibenzo[a,l]pyrene in HPLC Fractions via Room-Temperature Fluorescence Excitation–Emission Matrices
title_sort Unambiguous Determination of Benzo[a]pyrene and Dibenzo[a,l]pyrene in HPLC Fractions via Room-Temperature Fluorescence Excitation–Emission Matrices
dc.creator.none.fl_str_mv Knecht, George T.
Nauth, Stephanie D.
Gomez Alvarado, Juan C.
Santana, Anthony M.
Goicoechea, Hector Casimiro
Campiglia, Andres D.
author Knecht, George T.
author_facet Knecht, George T.
Nauth, Stephanie D.
Gomez Alvarado, Juan C.
Santana, Anthony M.
Goicoechea, Hector Casimiro
Campiglia, Andres D.
author_role author
author2 Nauth, Stephanie D.
Gomez Alvarado, Juan C.
Santana, Anthony M.
Goicoechea, Hector Casimiro
Campiglia, Andres D.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv dibenzo[a,l]pyrene
HPLC
room-temperature fluorescence
PARAFAC;
topic dibenzo[a,l]pyrene
HPLC
room-temperature fluorescence
PARAFAC;
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv When high-performance liquid chromatography (HPLC) is used for the analysis of polycyclic aromatic hydrocarbons (PAHs) in complex samples, further examination of HPLC fractions is recommended to confirm PAH assignments solely based on retention times. Gas chromatography–mass spectrometry (GC-MS) has been particularly relevant in the unambiguous determination of PAHs with remarkably similar retention times. The combination of HPLC and GC requires lengthy analysis times to ensure proper assignments. This article presents an approach for the analysis of co-eluted PAHs with no need for further chromatographic separation. Benzo[a]pyrene (BaP) and dibenzo[a,l]pyrene (DBalP) were directly determined in a co-eluted HPLC fraction via room-temperature fluorescence excitation–emission matrices (RTF-EEMs). RTF-EEMs can be recorded in a matter of seconds with a spectrofluorometer equipped with a multichannel detection system. The spectral overlapping of BaP and DBalP was resolved using parallel factor analysis (PARAFAC). The analytical advantages of this approach were demonstrated with the trace analysis (ng/mL) of these two PAHs in pre-concentrated tobacco extracts.
Fil: Knecht, George T.. University of Florida; Estados Unidos
Fil: Nauth, Stephanie D.. University of Florida; Estados Unidos
Fil: Gomez Alvarado, Juan C.. University of Florida; Estados Unidos
Fil: Santana, Anthony M.. University of Florida; Estados Unidos
Fil: Goicoechea, Hector Casimiro. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Campiglia, Andres D.. University of Florida; Estados Unidos
description When high-performance liquid chromatography (HPLC) is used for the analysis of polycyclic aromatic hydrocarbons (PAHs) in complex samples, further examination of HPLC fractions is recommended to confirm PAH assignments solely based on retention times. Gas chromatography–mass spectrometry (GC-MS) has been particularly relevant in the unambiguous determination of PAHs with remarkably similar retention times. The combination of HPLC and GC requires lengthy analysis times to ensure proper assignments. This article presents an approach for the analysis of co-eluted PAHs with no need for further chromatographic separation. Benzo[a]pyrene (BaP) and dibenzo[a,l]pyrene (DBalP) were directly determined in a co-eluted HPLC fraction via room-temperature fluorescence excitation–emission matrices (RTF-EEMs). RTF-EEMs can be recorded in a matter of seconds with a spectrofluorometer equipped with a multichannel detection system. The spectral overlapping of BaP and DBalP was resolved using parallel factor analysis (PARAFAC). The analytical advantages of this approach were demonstrated with the trace analysis (ng/mL) of these two PAHs in pre-concentrated tobacco extracts.
publishDate 2025
dc.date.none.fl_str_mv 2025-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/276456
Knecht, George T.; Nauth, Stephanie D.; Gomez Alvarado, Juan C.; Santana, Anthony M.; Goicoechea, Hector Casimiro; et al.; Unambiguous Determination of Benzo[a]pyrene and Dibenzo[a,l]pyrene in HPLC Fractions via Room-Temperature Fluorescence Excitation–Emission Matrices; Molecular Diversity Preservation International; Molecules; 30; 7; 3-2025; 1-11
1420-3049
CONICET Digital
CONICET
url http://hdl.handle.net/11336/276456
identifier_str_mv Knecht, George T.; Nauth, Stephanie D.; Gomez Alvarado, Juan C.; Santana, Anthony M.; Goicoechea, Hector Casimiro; et al.; Unambiguous Determination of Benzo[a]pyrene and Dibenzo[a,l]pyrene in HPLC Fractions via Room-Temperature Fluorescence Excitation–Emission Matrices; Molecular Diversity Preservation International; Molecules; 30; 7; 3-2025; 1-11
1420-3049
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1420-3049/30/7/1550
info:eu-repo/semantics/altIdentifier/doi/10.3390/molecules30071550
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Molecular Diversity Preservation International
publisher.none.fl_str_mv Molecular Diversity Preservation International
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.176297

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